BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0302a.1
(299 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [impo... 74 5e-14
BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At... 57 8e-12
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 62 2e-10
TC90516 61 5e-10
BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - ... 61 6e-10
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 53 2e-07
CB893203 44 6e-05
AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase... 43 1e-04
TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR re... 41 5e-04
TC82093 35 0.027
TC88830 homologue to GP|13878027|gb|AAK44091.1 unknown protein {... 29 1.9
BE942915 29 1.9
TC89491 similar to GP|1632831|emb|CAA89698.1 orf {Ricinus commun... 28 3.3
TC76993 homologue to PIR|T06463|T06463 phosphoribulokinase (EC 2... 28 4.3
CA990685 weakly similar to GP|22136772|gb putative carbonyl redu... 28 4.3
AW691821 similar to GP|10177570|db contains similarity to regula... 28 4.3
AW693179 weakly similar to GP|21594014|gb| unknown {Arabidopsis ... 28 5.6
BF641242 similar to GP|14595713|gb| putative impotin alpha 1b {O... 28 5.6
BI311602 similar to PIR|T48587|T48 peptide transporter-like prot... 27 7.3
TC91072 weakly similar to GP|22136510|gb|AAM91333.1 unknown prot... 27 9.5
>BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [imported] -
Arabidopsis thaliana, partial (7%)
Length = 687
Score = 74.3 bits (181), Expect = 5e-14
Identities = 53/190 (27%), Positives = 94/190 (48%), Gaps = 18/190 (9%)
Frame = +2
Query: 125 WVKDGDSNTKYFHNSVNWRRRTNAIRGLAVD-GEWVEDPKVVKSKMIEFFEAQFT-SNP- 181
W+KDGD NTK+FH+ + RR+ N I+ L + G W + + V+ +I +F FT SNP
Sbjct: 5 WLKDGDKNTKFFHSKASQRRKVNEIKKLKDETGNWCKGEENVERLLITYFNNLFTSSNPT 184
Query: 182 ---EVGVFLEGTPFRSISDEDNLALTQTFNLEEIRTAVWECEGDRSLGPD---------- 228
E ++G +S E + + F EE+ A+ + ++ GPD
Sbjct: 185 AIEETCEVVKG----KLSHEHIVWCEKEFTEEEVLEAINQMHPVKAPGPDGLPALFFQKY 352
Query: 229 --VLKVDILRVLKDFHRNGVWPKGGNSSFITLIPNISNLMSLNDYISISVIGCMYKIVSK 286
++ ++ +++ N + + N +FI LIP N + DY IS+ + KI++K
Sbjct: 353 WHIVGKEVQQMVLQVLNNSMETEELNKTFIVLIPKGKNPNTPKDYRPISLCNVVMKIITK 532
Query: 287 VLTTRLSKVM 296
V+ R+ + +
Sbjct: 533 VIANRVKQTL 562
>BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (16%)
Length = 794
Score = 57.0 bits (136), Expect(2) = 8e-12
Identities = 44/154 (28%), Positives = 71/154 (45%), Gaps = 16/154 (10%)
Frame = +3
Query: 119 QKSRDRWVKDGDSNTKYFHNSVNWRRRTNAIRGLAVDG--EWVEDPKVVKSKMIEFFEAQ 176
QKSR W++ GD NTK+FH RR N I L D EW + + ++ +
Sbjct: 141 QKSRLNWLRSGDRNTKFFHAVTKNRRAQNRILSLIDDDDKEWFVEEDL--GRLADSHFKL 314
Query: 177 FTSNPEVGVFLE--GTPFRSISDEDNLALTQTFNLEEIRTAVWECEGDRSLGPDVLKV-- 232
S+ +VG+ LE + +++E N L + EE+R AV++ + GPD + V
Sbjct: 315 LYSSEDVGITLEDWNSIPAIVTEEQNAQLMAQISREEVREAVFDINPHKCPGPDGMNVFF 494
Query: 233 ----------DILRVLKDFHRNGVWPKGGNSSFI 256
D+ + ++F R G +G N + I
Sbjct: 495 FQQFWDTMGDDLTSMAQEFLRTGKLEEGINKTNI 596
Score = 30.0 bits (66), Expect(2) = 8e-12
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +1
Query: 258 LIPNISNLMSLNDYISISVIGCMYKIVSKVLTTRLSKVM 296
L+P L ++ IS+ YKIVSKVL+ RL V+
Sbjct: 601 LVPKKLEAKRLVEFRPISLCNVAYKIVSKVLSKRLKSVL 717
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 62.4 bits (150), Expect = 2e-10
Identities = 30/64 (46%), Positives = 42/64 (64%)
Frame = +2
Query: 236 RVLKDFHRNGVWPKGGNSSFITLIPNISNLMSLNDYISISVIGCMYKIVSKVLTTRLSKV 295
R + +FHRN KG NS+FI LIP + N LND+ IS++G +YKI+ K+L RL V
Sbjct: 5 RFVSEFHRNRKLFKGINSTFIALIPKVDNPQRLNDFRPISLVGSLYKILGKLLANRLRVV 184
Query: 296 MGHI 299
+G +
Sbjct: 185 IGSV 196
>TC90516
Length = 983
Score = 61.2 bits (147), Expect = 5e-10
Identities = 56/214 (26%), Positives = 93/214 (43%), Gaps = 21/214 (9%)
Frame = -2
Query: 107 WKVAKFNDSLLFQKSRDRWVKDGDS---------NTKYFHNSVNWRRRTNAIRGLAVDGE 157
W + K LF +S+ R +G NT YF V R N+I V+
Sbjct: 829 WPICK-----LFCRSKTRRCSNGQEQSG*EREGHNTTYFLACVKNRGMRNSISAPRVE-R 668
Query: 158 WVEDPKVVKSKMIEFFEAQFTSNPEVGVFLEGTPFRSISDEDNLALTQTFNLEEIRTAVW 217
W++D K ++ FF F+ + F IS+ DN+ L+ F + ++ V
Sbjct: 667 WMDDVAKNKQAIVNFFTHHFSDP*TYRPTMGDIDFFHISNLDNVLLSAQFLVSKMELVVS 488
Query: 218 ECEGDRSLGPD------------VLKVDILRVLKDFHRNGVWPKGGNSSFITLIPNISNL 265
+G+ S PD +LK DI + + F+ K + F+TLI +
Sbjct: 487 SLDGNESPRPDGFNLNFFIRLRNMLKADIEIMFEQFYTPANLLKIFS*YFLTLISKVEYP 308
Query: 266 MSLNDYISISVIGCMYKIVSKVLTTRLSKVMGHI 299
+ L D+ +S +G + K+++KVL RL+ +M I
Sbjct: 307 ILLGDFSLMSFLGSL*KLMAKVLALRLAHIMEKI 206
>BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - Arabidopsis
thaliana, partial (17%)
Length = 677
Score = 60.8 bits (146), Expect = 6e-10
Identities = 39/130 (30%), Positives = 64/130 (49%), Gaps = 3/130 (2%)
Frame = +2
Query: 119 QKSRDRWVKDGDSNTKYFHNSVNWRRRTNAIRGL-AVDGEWVEDPKVVKSKMIEFFEAQF 177
QKSR+ W GD N K++H R N I GL DG W+ + + V+ +++FE F
Sbjct: 59 QKSRNMWHISGDLNKKFYHALTKQRHARNRIVGLYDYDGNWITEEQGVEKVAVDYFEDLF 238
Query: 178 TSNPEVGV--FLEGTPFRSISDEDNLALTQTFNLEEIRTAVWECEGDRSLGPDVLKVDIL 235
G FL+ SI+ + N L + EE+R A++ +++ GPD + +
Sbjct: 239 QRTTPTGFDGFLDEIT-SSITPQMNQRLLRLATEEEVRLALFIMHPEKAPGPDGMTTLLF 415
Query: 236 RVLKDFHRNG 245
L +++NG
Sbjct: 416 STLMAYNKNG 445
Score = 37.4 bits (85), Expect = 0.007
Identities = 21/64 (32%), Positives = 35/64 (53%)
Frame = +3
Query: 229 VLKVDILRVLKDFHRNGVWPKGGNSSFITLIPNISNLMSLNDYISISVIGCMYKIVSKVL 288
++K+D+L+++ F +G N++ I LIP + + IS+ YKI+SKVL
Sbjct: 432 IIKMDLLKMVNSFLASGKLDTRLNTTNICLIPKKKRPTRMTELRPISLCNVGYKIISKVL 611
Query: 289 TTRL 292
RL
Sbjct: 612 CQRL 623
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 52.8 bits (125), Expect = 2e-07
Identities = 28/109 (25%), Positives = 55/109 (49%), Gaps = 12/109 (11%)
Frame = +3
Query: 203 LTQTFNLEEIRTAVWECEGDRSLGPDVLKV------------DILRVLKDFHRNGVWPKG 250
L F E++ A++ + ++ G D V ++ + DF + G PK
Sbjct: 12 LCSEFTAVEVKNALFSMDSSKAPGIDGYNVHFFKCSWNIIGDSVIDAILDFFKTGFMPKI 191
Query: 251 GNSSFITLIPNISNLMSLNDYISISVIGCMYKIVSKVLTTRLSKVMGHI 299
N +++TL+P N+ S+ ++ I+ +YKI+SK+LT+R+ V+ +
Sbjct: 192 INCTYVTLLPKEVNVTSVKNFRPIACCSVIYKIISKILTSRMQGVLNSV 338
>CB893203
Length = 800
Score = 44.3 bits (103), Expect = 6e-05
Identities = 25/78 (32%), Positives = 34/78 (43%), Gaps = 1/78 (1%)
Frame = -3
Query: 19 CWLQNHAFGECVST-WSSFDIQGWGAYTFKEKLKRLKLKLNQWEKENVGDLDKICKDPVT 77
CW + V W+SF + WG Y KEK K ++ L W + +LD D
Sbjct: 663 CWADLPGYHLFVKDKWNSFQVSRWGGYVLKEK*KMVRNNLKDWHHNHTRNLDARINDIRE 484
Query: 78 RINALDIKEGDGNLSSQE 95
I LD K D L ++E
Sbjct: 483 SIAYLDSKGEDLTLDTEE 430
>AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (4%)
Length = 305
Score = 43.1 bits (100), Expect = 1e-04
Identities = 22/58 (37%), Positives = 31/58 (52%), Gaps = 14/58 (24%)
Frame = +1
Query: 207 FNLEEIRTAVWECEGDRSLGP--------------DVLKVDILRVLKDFHRNGVWPKG 250
F+ EE+R AVW+C+ S P +++KVD LRV+ +FH NG KG
Sbjct: 121 FSEEEVRKAVWDCDSSHS*NPGPDGVNFTFVKEYWELIKVDFLRVVMEFHTNGKLGKG 294
>TC81386 similar to GP|10140689|gb|AAG13524.1 putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 798
Score = 41.2 bits (95), Expect = 5e-04
Identities = 25/117 (21%), Positives = 46/117 (38%)
Frame = -1
Query: 33 WSSFDIQGWGAYTFKEKLKRLKLKLNQWEKENVGDLDKICKDPVTRINALDIKEGDGNLS 92
W++ + G + +KLK LK L W K G++ + ++ + +K S
Sbjct: 351 WAN-QVVGCPMFVLNQKLKNLKEVLKVWNKNTFGNVHSQVDNAYKELDDIQVKIDSIGYS 175
Query: 93 SQEQKQRDDFLVEYWKVAKFNDSLLFQKSRDRWVKDGDSNTKYFHNSVNWRRRTNAI 149
Q + + +KS+ W +GD NT +FH +R ++ I
Sbjct: 174 DVLMDQEKAAQLNLESALNIEEVFWHEKSKVNWHCEGDRNTAFFHRVAKIKRTSSLI 4
>TC82093
Length = 569
Score = 35.4 bits (80), Expect = 0.027
Identities = 22/49 (44%), Positives = 29/49 (58%), Gaps = 1/49 (2%)
Frame = -1
Query: 108 KVAKFNDSLLFQKSRDRWVKDGDSNTKYFHN-SVNWRRRTNAIRGLAVD 155
K+ N+ L + KS+ WVK G NT +FHN VN R +T+ GL VD
Sbjct: 479 KLLSQNEMLWY*KSQ*NWVKYGSRNTWFFHNEDVN*RNQTS---GLIVD 342
>TC88830 homologue to GP|13878027|gb|AAK44091.1 unknown protein {Arabidopsis
thaliana}, partial (36%)
Length = 721
Score = 29.3 bits (64), Expect = 1.9
Identities = 18/93 (19%), Positives = 39/93 (41%)
Frame = +3
Query: 206 TFNLEEIRTAVWECEGDRSLGPDVLKVDILRVLKDFHRNGVWPKGGNSSFITLIPNISNL 265
T E ++ +W E +++LG + +I + G+W + I I L
Sbjct: 294 TIRTEAVKGCIWLFEQEQALGFSSVSTNIRMTVIKLKSGGLWVHAPIAPTDECIQLIKEL 473
Query: 266 MSLNDYISISVIGCMYKIVSKVLTTRLSKVMGH 298
+ +YI + +KI+ + + ++S G+
Sbjct: 474 GAPVEYIVLPTFAYEHKIICRSVFKKISACSGY 572
>BE942915
Length = 328
Score = 29.3 bits (64), Expect = 1.9
Identities = 16/49 (32%), Positives = 25/49 (50%)
Frame = +1
Query: 23 NHAFGECVSTWSSFDIQGWGAYTFKEKLKRLKLKLNQWEKENVGDLDKI 71
N + + ++T S+FD W + +K LKL W EN+G+ KI
Sbjct: 115 NSSLSQFLNTASTFD---WVVGCYTSTMKITFLKLGLWRIENIGENGKI 252
>TC89491 similar to GP|1632831|emb|CAA89698.1 orf {Ricinus communis},
partial (97%)
Length = 727
Score = 28.5 bits (62), Expect = 3.3
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = +2
Query: 255 FITLIPNISNLMSLNDYISISVIGC 279
F T+ N++ +M LN YIS+S++GC
Sbjct: 563 FYTVEANLTAVM*LNLYISLSLVGC 637
>TC76993 homologue to PIR|T06463|T06463 phosphoribulokinase (EC 2.7.1.19) -
garden pea (fragment), complete
Length = 1577
Score = 28.1 bits (61), Expect = 4.3
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = +2
Query: 248 PKGGNSSFITLIPNISNLMSLNDYISISVIG 278
PKGGN TLI + + ++ L+DY S+ G
Sbjct: 398 PKGGNPDSNTLISDTTTVICLDDYHSLDRTG 490
>CA990685 weakly similar to GP|22136772|gb putative carbonyl reductase
{Arabidopsis thaliana}, partial (36%)
Length = 554
Score = 28.1 bits (61), Expect = 4.3
Identities = 12/31 (38%), Positives = 20/31 (63%)
Frame = +2
Query: 49 KLKRLKLKLNQWEKENVGDLDKICKDPVTRI 79
KLKR+K N+W +E GD+D + ++ V +
Sbjct: 260 KLKRIK---NEWTREVFGDVDNLTEEKVDEV 343
>AW691821 similar to GP|10177570|db contains similarity to regulatory protein
Nedd1~gene_id:K18J17.16 {Arabidopsis thaliana}, partial
(19%)
Length = 648
Score = 28.1 bits (61), Expect = 4.3
Identities = 16/50 (32%), Positives = 30/50 (60%), Gaps = 2/50 (4%)
Frame = -1
Query: 159 VEDPKVVKSKMIEFFEAQFTSNPEVGVFL--EGTPFRSISDEDNLALTQT 206
+ P + +K + F + T NPEVGV L +G+P +++E++ +L +T
Sbjct: 324 LSSPALATTKFV*FHLTELT*NPEVGVKLRVQGSP-PGLTEEESKSLPET 178
>AW693179 weakly similar to GP|21594014|gb| unknown {Arabidopsis thaliana},
partial (46%)
Length = 409
Score = 27.7 bits (60), Expect = 5.6
Identities = 12/31 (38%), Positives = 20/31 (63%)
Frame = +1
Query: 202 ALTQTFNLEEIRTAVWECEGDRSLGPDVLKV 232
A+ T++L E+ +VWE +L P+VL+V
Sbjct: 316 AIVATYSLFEMSASVWEISRGATLFPEVLQV 408
>BF641242 similar to GP|14595713|gb| putative impotin alpha 1b {Oryza
sativa}, partial (11%)
Length = 680
Score = 27.7 bits (60), Expect = 5.6
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +1
Query: 44 YTFKEKLKRLKLKLNQWEKENVG 66
YT + + LKLKLN W K +VG
Sbjct: 109 YTLELVILVLKLKLNSWRKSSVG 177
>BI311602 similar to PIR|T48587|T48 peptide transporter-like protein -
Arabidopsis thaliana, partial (29%)
Length = 555
Score = 27.3 bits (59), Expect = 7.3
Identities = 16/52 (30%), Positives = 24/52 (45%)
Frame = +1
Query: 225 LGPDVLKVDILRVLKDFHRNGVWPKGGNSSFITLIPNISNLMSLNDYISISV 276
LG +L V + + + RN +SF+T +PN+S L Y I V
Sbjct: 289 LGVSILSVAWAAIFEKYRRNYAIKNEFEASFLTPMPNLSAYWLLIQYCLIGV 444
>TC91072 weakly similar to GP|22136510|gb|AAM91333.1 unknown protein
{Arabidopsis thaliana}, partial (15%)
Length = 1021
Score = 26.9 bits (58), Expect = 9.5
Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 7/60 (11%)
Frame = +2
Query: 52 RLKLKLNQWEKENVGDLDKICKDPVTRIN--ALDIKEGDGNLS-----SQEQKQRDDFLV 104
+LK K + EKEN + DK+ V + + IK+ +G +S S E+ + DD +V
Sbjct: 824 KLKEKEKEKEKENENEKDKLHPSQVLEKDDQQIPIKDNNGKVSDVRQQSNEESEADDDMV 1003
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.140 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,903,975
Number of Sequences: 36976
Number of extensions: 138821
Number of successful extensions: 889
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 878
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 881
length of query: 299
length of database: 9,014,727
effective HSP length: 96
effective length of query: 203
effective length of database: 5,465,031
effective search space: 1109401293
effective search space used: 1109401293
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0302a.1