BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0299.12
(418 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78573 similar to GP|20197309|gb|AAC63626.2 unknown protein {Ar... 716 0.0
TC83851 similar to GP|15027849|gb|AAK76455.1 unknown protein {Ar... 206 1e-53
TC76600 similar to GP|20197309|gb|AAC63626.2 unknown protein {Ar... 180 8e-46
TC90788 similar to PIR|F86340|F86340 protein F2D10.34 [imported]... 42 6e-04
TC78547 similar to GP|12003386|gb|AAG43550.1 Avr9/Cf-9 rapidly e... 39 0.004
TC88172 similar to GP|12003386|gb|AAG43550.1 Avr9/Cf-9 rapidly e... 38 0.006
TC78520 similar to PIR|T47637|T47637 hypothetical protein T5N23.... 37 0.014
TC90096 similar to PIR|T47832|T47832 hypothetical protein T2O9.6... 37 0.018
TC91850 similar to GP|22597166|gb|AAN03470.1 RING-H2 finger prot... 37 0.018
TC84165 similar to GP|21539497|gb|AAM53301.1 putative protein {A... 36 0.024
TC88616 weakly similar to PIR|T48209|T48209 hypothetical protein... 36 0.024
AJ499045 similar to PIR|F86340|F86 protein F2D10.34 [imported] -... 35 0.041
BF646214 similar to GP|8843779|dbj| gene_id:MZN1.3~unknown prote... 35 0.041
TC93425 similar to GP|15450675|gb|AAK96609.1 At1g26800/T24P13_21... 35 0.054
TC87325 similar to GP|18176187|gb|AAL60000.1 unknown protein {Ar... 35 0.054
TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2... 35 0.070
TC85547 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2... 35 0.070
TC88854 similar to GP|7767667|gb|AAF69164.1| F27F5.26 {Arabidops... 34 0.092
TC78411 similar to PIR|T47692|T47692 hypothetical protein T22E16... 34 0.12
TC81271 weakly similar to GP|20197250|gb|AAC23649.2 Expressed pr... 34 0.12
>TC78573 similar to GP|20197309|gb|AAC63626.2 unknown protein {Arabidopsis
thaliana}, partial (31%)
Length = 2016
Score = 716 bits (1848), Expect = 0.0
Identities = 342/429 (79%), Positives = 372/429 (85%), Gaps = 11/429 (2%)
Frame = +2
Query: 1 MGLGNNEDEGNQEVDGGD--GGGGKGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDC 58
MGLGN E++ N + G K GSVSCSICLEVV DNGDRS+AKL CGHQFHLDC
Sbjct: 173 MGLGNEEEDDNNNHNHNKVVDEGNKSLGSVSCSICLEVVTDNGDRSFAKLLCGHQFHLDC 352
Query: 59 IGSAFNIKGAMQCPNCRKIEKGQWLYANGGRSYPEFNMDDWTYDEDLYDLSYSELQSFGV 118
IGSAFNIKGAMQCPNCRKIEKGQWLYANG RSYPEF+MDDWT+DEDLYDLSYSE+ SFGV
Sbjct: 353 IGSAFNIKGAMQCPNCRKIEKGQWLYANGSRSYPEFSMDDWTHDEDLYDLSYSEM-SFGV 529
Query: 119 HWCPFGNLARLPSPFDH---------DMLGQHAIFAEHTAVSSGSHHCPYIAYVGPIHPS 169
HWCPFGN+ARLPS F+ D++GQHA FAEHTAVSS SH CPY+AY GPIHPS
Sbjct: 530 HWCPFGNMARLPSSFEEGEFSSIAYPDIIGQHAAFAEHTAVSSASHPCPYVAYFGPIHPS 709
Query: 170 SSNSGGTVSEASNFNHWNGSSVPNDMPASFTFPAVDLHYHGWEHHSPPFSTASSRLVAAD 229
SSNSGG VSEA NFNHWNGSSVP++MPAS+TFPAVDLHYH WEHH+PPFSTASSRLV AD
Sbjct: 710 SSNSGGAVSEAPNFNHWNGSSVPSEMPASYTFPAVDLHYHSWEHHTPPFSTASSRLVGAD 889
Query: 230 QPSVSPGNQRPVRGGSDVPRSGSFMHPFLVGHSSAARAGSSVASSLIPPYPGSNARARDR 289
QPSVSP +QRPV+GGSDVPRSGSFMHPFLVGHSSAARAGSS+ SS+IPPYPGSNARARDR
Sbjct: 890 QPSVSPASQRPVKGGSDVPRSGSFMHPFLVGHSSAARAGSSMGSSMIPPYPGSNARARDR 1069
Query: 290 VQALQAYYQPQQPPNSTTMRTPIASGTRRPSIHGGSAPLAPVATSLDQSGGFFLIPPSSS 349
VQALQAYYQPQQ PNSTTMR P++S RR S H GS LAPVATS DQSGGFFLIP SSS
Sbjct: 1070VQALQAYYQPQQHPNSTTMRAPVSSVPRRASSHSGSTQLAPVATSPDQSGGFFLIPSSSS 1249
Query: 350 GRNFQEENHLPSRYHAWERDHLPSLSLNHVDRDSGWRAYHQTFSRPDPATRSSSFRSRHG 409
GRNFQEENHLPSR+HAWERDHLPSLSL+ DRDSGWR YHQ+ +R DP+TRS++FR RHG
Sbjct: 1250GRNFQEENHLPSRFHAWERDHLPSLSLSQGDRDSGWRPYHQSANRSDPSTRSNNFRLRHG 1429
Query: 410 SDRMPSQNR 418
SDRMPSQ+R
Sbjct: 1430SDRMPSQHR 1456
>TC83851 similar to GP|15027849|gb|AAK76455.1 unknown protein {Arabidopsis
thaliana}, partial (14%)
Length = 674
Score = 206 bits (525), Expect = 1e-53
Identities = 118/223 (52%), Positives = 136/223 (60%), Gaps = 3/223 (1%)
Frame = +1
Query: 158 PYIAYVGPIHPSSSNSGGTVSEASNFNHWNGSSVPNDMPASFTFPAVDLHYHGWEHHSPP 217
PY GP+ S SE SNFNHWN + DM S+T PAV LHYH W+HHS
Sbjct: 34 PYHDAPGPMFTEHS---AVSSEVSNFNHWNARPIHGDMSTSYTVPAVVLHYHSWDHHSSH 204
Query: 218 FSTASSRLVAADQPSVSPGNQRPVRGGSDVPRSGSFMHPFLVGHSSAARAGSSVASSLIP 277
FS+ SS L AADQPSVS NQRP RG S+ RSGS+MHP+ VGHSS ARAG+S ASS+IP
Sbjct: 205 FSSGSSHLGAADQPSVSQSNQRPTRG-SEATRSGSYMHPYPVGHSSVARAGNSAASSMIP 381
Query: 278 PYPGSNARARDRVQALQAYYQPQQPP---NSTTMRTPIASGTRRPSIHGGSAPLAPVATS 334
PYPGSNARAR+RV+ALQAYY QQP +ST +A P++ G
Sbjct: 382 PYPGSNARARNRVEALQAYYHQQQPSKFYHSTATCRFLA--LEDPAVTVGQLH*HRCPRR 555
Query: 335 LDQSGGFFLIPPSSSGRNFQEENHLPSRYHAWERDHLPSLSLN 377
+ F+ S FQEE LPS HAWERDHLPS LN
Sbjct: 556 QTKVVATFI----SQDAIFQEETRLPSHXHAWERDHLPSSXLN 672
Score = 29.3 bits (64), Expect = 2.9
Identities = 25/88 (28%), Positives = 39/88 (43%)
Frame = +1
Query: 149 AVSSGSHHCPYIAYVGPIHPSSSNSGGTVSEASNFNHWNGSSVPNDMPASFTFPAVDLHY 208
A SGS+ PY P+ SS G + +S + GS+ A A+ +Y
Sbjct: 289 ATRSGSYMHPY-----PVGHSSVARAGNSAASSMIPPYPGSNAR----ARNRVEALQAYY 441
Query: 209 HGWEHHSPPFSTASSRLVAADQPSVSPG 236
H + STA+ R +A + P+V+ G
Sbjct: 442 HQQQPSKFYHSTATCRFLALEDPAVTVG 525
>TC76600 similar to GP|20197309|gb|AAC63626.2 unknown protein {Arabidopsis
thaliana}, partial (17%)
Length = 505
Score = 180 bits (457), Expect = 8e-46
Identities = 84/108 (77%), Positives = 92/108 (84%), Gaps = 2/108 (1%)
Frame = +3
Query: 14 VDGGDGGGG--KGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQC 71
+D GDG K SV CSICLE+V+DNGDRS+AKL CGHQFHLDCIGSAFN KGAMQC
Sbjct: 183 IDDGDGDTRHRKSSDSVPCSICLEIVSDNGDRSFAKLQCGHQFHLDCIGSAFNAKGAMQC 362
Query: 72 PNCRKIEKGQWLYANGGRSYPEFNMDDWTYDEDLYDLSYSELQSFGVH 119
PNCRKIEKGQWLYANG RSYPEFNM+DWT D+D+YDLSYSE+ S GVH
Sbjct: 363 PNCRKIEKGQWLYANGSRSYPEFNMEDWTRDDDVYDLSYSEM-SLGVH 503
>TC90788 similar to PIR|F86340|F86340 protein F2D10.34 [imported] -
Arabidopsis thaliana, partial (52%)
Length = 745
Score = 41.6 bits (96), Expect = 6e-04
Identities = 27/79 (34%), Positives = 36/79 (45%), Gaps = 6/79 (7%)
Frame = +2
Query: 18 DGGGGKGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKI 77
D GK + C+ICL A GD CGH FH+ CI + + CP+CR+I
Sbjct: 299 DSNPGKWLATTECAICLSDFAA-GDEIRVLPQCGHGFHVACIDTWLGSHSS--CPSCRQI 469
Query: 78 ------EKGQWLYANGGRS 90
+K ANGG +
Sbjct: 470 LAVTRCQKCGRFPANGGEA 526
>TC78547 similar to GP|12003386|gb|AAG43550.1 Avr9/Cf-9 rapidly elicited
protein 132 {Nicotiana tabacum}, partial (41%)
Length = 1648
Score = 38.9 bits (89), Expect = 0.004
Identities = 54/213 (25%), Positives = 87/213 (40%), Gaps = 10/213 (4%)
Frame = +2
Query: 28 VSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKIEKGQWLYANG 87
+ C++CL V + G+++ C H FHLDCI F + CP CR + + N
Sbjct: 620 LECAVCLCDVVE-GEKTRLLPKCNHGFHLDCIDMWF--QSHSTCPLCRNLVSVESCKPNS 790
Query: 88 GRSY-PEFNMDDWTYDEDLYDLSYSELQSFGVHWCPFGNLARLPSPFDHDMLGQHAIFAE 146
S E N+ + E+L ++ E +F + +GN R S +
Sbjct: 791 TTSNGEEMNVLVSSEGENLSHVNGLESSNFPTNVLVWGNSQRQVSS------------SF 934
Query: 147 HTAVSSGSHHCPYIAYVGPIHPSSSNSGGTVSEASNFNHWNGSS-------VPNDMPASF 199
++ GS H + +SS+S S+ N NG+ +P+D +S
Sbjct: 935 GVSLEEGSSHQQPCS-------TSSSSSTLGSDNGNSGRCNGNGMLVIDIPLPSDFSSSS 1093
Query: 200 TFPAVDLHYHGWEHHSPPFSTASS--RLVAADQ 230
P+ D Y E SP S S RL++ D+
Sbjct: 1094LSPS-DNRYVEDEMKSPMASRLRSFKRLLSRDK 1189
>TC88172 similar to GP|12003386|gb|AAG43550.1 Avr9/Cf-9 rapidly elicited
protein 132 {Nicotiana tabacum}, partial (26%)
Length = 1445
Score = 38.1 bits (87), Expect = 0.006
Identities = 26/88 (29%), Positives = 35/88 (39%), Gaps = 17/88 (19%)
Frame = +3
Query: 5 NNEDEGNQEVDGGDGGG-----------------GKGFGSVSCSICLEVVADNGDRSWAK 47
NN + G +GGG GF + CS+CL V + G++
Sbjct: 369 NNSQSRRRRRSGQEGGGLDPSVLNSLPISIFNSKEDGF-DLECSVCLSEVVE-GEKVRVL 542
Query: 48 LHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
C H FH+DCI F+ CP CR
Sbjct: 543 PKCNHMFHIDCIDMWFHSHST--CPLCR 620
>TC78520 similar to PIR|T47637|T47637 hypothetical protein T5N23.140 -
Arabidopsis thaliana, partial (71%)
Length = 2748
Score = 37.0 bits (84), Expect = 0.014
Identities = 18/48 (37%), Positives = 24/48 (49%), Gaps = 1/48 (2%)
Frame = +1
Query: 29 SCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIK-GAMQCPNCR 75
+C+ICL + ++ C H FH CI S N+K G CP CR
Sbjct: 358 TCAICLTKMKQGSGQAIFTAECSHSFHFHCIAS--NVKHGNQVCPVCR 495
>TC90096 similar to PIR|T47832|T47832 hypothetical protein T2O9.60 -
Arabidopsis thaliana, partial (13%)
Length = 676
Score = 36.6 bits (83), Expect = 0.018
Identities = 19/50 (38%), Positives = 26/50 (52%)
Frame = +1
Query: 26 GSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
G V C++C E V+ D +L C H +H DCI I+ + CP CR
Sbjct: 364 GVVICAVCKETVSV--DEEAKQLPCDHLYHADCITPWLRIRSS--CPLCR 501
>TC91850 similar to GP|22597166|gb|AAN03470.1 RING-H2 finger protein
{Glycine max}, partial (48%)
Length = 1054
Score = 36.6 bits (83), Expect = 0.018
Identities = 20/47 (42%), Positives = 25/47 (52%)
Frame = +3
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRK 76
C ICLE D GD+ L CGH+FH C+ I+ CP CR+
Sbjct: 609 CGICLESFMD-GDKL-IHLQCGHKFHSACLDPW--IRSCGDCPYCRR 737
>TC84165 similar to GP|21539497|gb|AAM53301.1 putative protein {Arabidopsis
thaliana}, partial (16%)
Length = 792
Score = 36.2 bits (82), Expect = 0.024
Identities = 18/48 (37%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Frame = +3
Query: 29 SCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIK-GAMQCPNCR 75
+C+ICL + + C H FH CI S N+K G CP CR
Sbjct: 57 TCAICLNTMKPGNGHAIFTAECSHSFHFHCITS--NVKHGNQICPVCR 194
>TC88616 weakly similar to PIR|T48209|T48209 hypothetical protein T20L15.150
- Arabidopsis thaliana, partial (27%)
Length = 1148
Score = 36.2 bits (82), Expect = 0.024
Identities = 20/52 (38%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +2
Query: 28 VSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR-KIE 78
V C++CL VV D + +C H FH+ CI + CPNCR K+E
Sbjct: 497 VECAVCLSVVEDE-EMMRLLPNCKHSFHVGCIDKW--LASHSTCPNCRTKVE 643
>AJ499045 similar to PIR|F86340|F86 protein F2D10.34 [imported] - Arabidopsis
thaliana, partial (62%)
Length = 692
Score = 35.4 bits (80), Expect = 0.041
Identities = 18/48 (37%), Positives = 24/48 (49%)
Frame = +2
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKI 77
C+ICL GD CGH FH+ CI ++ CP+CR+I
Sbjct: 371 CAICLSEFTA-GDEIRVLPQCGHGFHVSCID--LWLRSHSSCPSCRQI 505
>BF646214 similar to GP|8843779|dbj| gene_id:MZN1.3~unknown protein
{Arabidopsis thaliana}, partial (27%)
Length = 638
Score = 35.4 bits (80), Expect = 0.041
Identities = 26/80 (32%), Positives = 34/80 (42%)
Frame = +2
Query: 28 VSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKIEKGQWLYANG 87
+ C ICL D G+ CGH FHL+CI + CP CR ++ N
Sbjct: 374 LECVICLSGFED-GEMGRCLPKCGHGFHLECID--MWLSSHSNCPICR---ASIVVFEND 535
Query: 88 GRSYPEFNMDDWTYDEDLYD 107
S E N DD + E + D
Sbjct: 536 SSSVVENNGDDSSIVEIVVD 595
>TC93425 similar to GP|15450675|gb|AAK96609.1 At1g26800/T24P13_21
{Arabidopsis thaliana}, partial (28%)
Length = 596
Score = 35.0 bits (79), Expect = 0.054
Identities = 22/63 (34%), Positives = 29/63 (45%)
Frame = +2
Query: 13 EVDGGDGGGGKGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCP 72
E++ GDGG C +CLE G ++ C H+FH CI I G+ CP
Sbjct: 359 EIEEGDGG--------ECVVCLEEFEVGGVVK--EMPCKHRFHGKCIEKWLGIHGS--CP 502
Query: 73 NCR 75
CR
Sbjct: 503 VCR 511
>TC87325 similar to GP|18176187|gb|AAL60000.1 unknown protein {Arabidopsis
thaliana}, partial (50%)
Length = 1568
Score = 35.0 bits (79), Expect = 0.054
Identities = 19/49 (38%), Positives = 25/49 (50%), Gaps = 2/49 (4%)
Frame = +3
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFN--IKGAMQCPNCRK 76
C+IC E +A KL+C H FHL C+ S + + CP CRK
Sbjct: 336 CAICREPMAKA-----KKLNCNHLFHLACLRSWLDQGLTEMYTCPTCRK 467
>TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
[imported] - Arabidopsis thaliana, partial (69%)
Length = 1554
Score = 34.7 bits (78), Expect = 0.070
Identities = 19/46 (41%), Positives = 24/46 (51%)
Frame = +3
Query: 29 SCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNC 74
SCSICLE D+ + C H+FHL CI + + QCP C
Sbjct: 195 SCSICLEEFIDSDPSTVTS--CKHEFHLQCILEW--CQRSSQCPMC 320
>TC85547 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
[imported] - Arabidopsis thaliana, partial (42%)
Length = 677
Score = 34.7 bits (78), Expect = 0.070
Identities = 19/46 (41%), Positives = 24/46 (51%)
Frame = +2
Query: 29 SCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNC 74
SCSICLE D+ + C H+FHL CI + + QCP C
Sbjct: 194 SCSICLEEFIDSDPSTVTS--CKHEFHLQCILEW--CQRSSQCPMC 319
>TC88854 similar to GP|7767667|gb|AAF69164.1| F27F5.26 {Arabidopsis
thaliana}, partial (22%)
Length = 1043
Score = 34.3 bits (77), Expect = 0.092
Identities = 20/56 (35%), Positives = 25/56 (43%), Gaps = 4/56 (7%)
Frame = +2
Query: 24 GFGSVS----CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
G GS S C +C E + D L CGH +H+DCI K CP C+
Sbjct: 557 GLGSQSETEPCCVCQEEFKNEDDIG--SLDCGHDYHIDCIKQWLTHKNI--CPICK 712
>TC78411 similar to PIR|T47692|T47692 hypothetical protein T22E16.190 -
Arabidopsis thaliana, partial (81%)
Length = 1378
Score = 33.9 bits (76), Expect = 0.12
Identities = 19/48 (39%), Positives = 24/48 (49%)
Frame = +2
Query: 28 VSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
++CS+CLE V N L C HQFH CI +G CP C+
Sbjct: 872 LTCSVCLEQV--NVGDVLRSLPCLHQFHASCIDPWLRQQGT--CPVCK 1003
>TC81271 weakly similar to GP|20197250|gb|AAC23649.2 Expressed protein
{Arabidopsis thaliana}, partial (30%)
Length = 870
Score = 33.9 bits (76), Expect = 0.12
Identities = 19/47 (40%), Positives = 21/47 (44%)
Frame = +1
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRK 76
C +CL V AD G+ C H FH CI N CP CRK
Sbjct: 472 CPVCLSVFAD-GEEVRQLSVCKHSFHTSCIDLWLNDHN--NCPICRK 603
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.133 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,132,311
Number of Sequences: 36976
Number of extensions: 281729
Number of successful extensions: 2034
Number of sequences better than 10.0: 119
Number of HSP's better than 10.0 without gapping: 1949
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2014
length of query: 418
length of database: 9,014,727
effective HSP length: 99
effective length of query: 319
effective length of database: 5,354,103
effective search space: 1707958857
effective search space used: 1707958857
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0299.12