BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0290.12
(143 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89314 similar to GP|21617911|gb|AAM66961.1 unknown {Arabidopsi... 206 3e-54
TC85272 similar to SP|Q02060|PSBS_SPIOL Photosystem II 22 kDa pr... 39 7e-04
TC79576 similar to GP|14423380|gb|AAK62372.1 Unknown protein {Ar... 37 0.004
TC76978 homologue to GP|14487954|gb|AAK63815.1 early light induc... 36 0.006
TC76979 homologue to GP|14487954|gb|AAK63815.1 early light induc... 36 0.006
TC76980 similar to GP|14487954|gb|AAK63815.1 early light inducib... 35 0.013
TC90438 weakly similar to GP|21592719|gb|AAM64668.1 unknown {Ara... 33 0.039
TC90281 similar to SP|P54225|HEMZ_SYNY3 Ferrochelatase (EC 4.99.... 32 0.066
TC86769 similar to GP|14190393|gb|AAK55677.1 At1g34000/F12G12_21... 32 0.066
TC79896 similar to GP|15795154|dbj|BAB03142. gene_id:MQC3.16~unk... 30 0.25
AW774435 homologue to GP|20146255|dbj hypothetical protein {Oryz... 30 0.33
AW774436 homologue to GP|20146255|dbj hypothetical protein {Oryz... 30 0.33
TC88385 similar to GP|22136512|gb|AAM91334.1 unknown protein {Ar... 28 0.96
BQ144045 similar to PIR|T51947|T51 probable transcription factor... 28 1.6
BG450637 similar to SP|P14895|ELI5_ High molecular mass early li... 27 2.1
BQ750994 similar to GP|15408680|db putative multi resistance pro... 27 3.6
TC77155 homologue to GP|15028015|gb|AAK76538.1 unknown protein {... 27 3.6
TC87694 similar to PIR|T48233|T48233 one helix protein (OHP) - A... 27 3.6
TC76410 similar to GP|4689382|gb|AAD27878.1| chlorophyll a/b bin... 27 3.6
TC76507 homologue to GP|506629|gb|AAA50172.1|| photosystem II ty... 26 4.7
>TC89314 similar to GP|21617911|gb|AAM66961.1 unknown {Arabidopsis
thaliana}, partial (52%)
Length = 755
Score = 206 bits (524), Expect = 3e-54
Identities = 109/145 (75%), Positives = 123/145 (84%), Gaps = 2/145 (1%)
Frame = +2
Query: 1 MALA-TQTSASLSISFRDACVFPAAKYSAT-ARFPLPNLVPSRASFATGTPLLIRRSHGR 58
MALA Q SASL +S RDA VF A AT A+FP P LVP+RASFATG+PL+IR +H R
Sbjct: 167 MALAQAQPSASLPVSLRDARVFTARNNPATSAQFPKPTLVPARASFATGSPLMIRSNHKR 346
Query: 59 KLACRAMPVSIRSEQSTKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLP 118
KLAC+AMPVSIR EQ+TKDGNGLDVWLGR+AM+GFA ITVE+ +GKGLLENFG+ SPLP
Sbjct: 347 KLACKAMPVSIRCEQNTKDGNGLDVWLGRVAMVGFAVAITVEITSGKGLLENFGLTSPLP 526
Query: 119 TVALAVTALVGVLTAVFIFQSASKN 143
TVALAVT LVGVLTAVFIFQSAS+N
Sbjct: 527 TVALAVTGLVGVLTAVFIFQSASEN 601
>TC85272 similar to SP|Q02060|PSBS_SPIOL Photosystem II 22 kDa protein
chloroplast precursor (CP22). [Spinach] {Spinacia
oleracea}, partial (76%)
Length = 970
Score = 38.9 bits (89), Expect = 7e-04
Identities = 17/37 (45%), Positives = 26/37 (69%)
Frame = +3
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLP 118
++++GR+AMIGFAA I E TGKG+L + + +P
Sbjct: 360 ELFVGRVAMIGFAASILGEALTGKGILAQLNLETGIP 470
Score = 35.4 bits (80), Expect = 0.008
Identities = 18/59 (30%), Positives = 31/59 (52%)
Frame = +3
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFIFQSA 140
++++GRLA +GF + E+ TGKG L + + +P + L V +F F +A
Sbjct: 666 ELFVGRLAQLGFVFSLIGEIITGKGALAQLNIETGVPITEIEPLVLFNV---IFFFIAA 833
>TC79576 similar to GP|14423380|gb|AAK62372.1 Unknown protein {Arabidopsis
thaliana}, partial (75%)
Length = 795
Score = 36.6 bits (83), Expect = 0.004
Identities = 21/70 (30%), Positives = 37/70 (52%)
Frame = +1
Query: 73 QSTKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLT 132
+S+K ++ GRLAM+ FAA +TVE+ TG + +A + A+ L
Sbjct: 328 ESSKKSQDFEIISGRLAMMVFAATVTVELVTGNSVFRKMDIAGITEAGGICFGAV--TLA 501
Query: 133 AVFIFQSASK 142
A+F + S+++
Sbjct: 502 AMFAWFSSAR 531
>TC76978 homologue to GP|14487954|gb|AAK63815.1 early light inducible
protein {Medicago sativa}, complete
Length = 953
Score = 35.8 bits (81), Expect = 0.006
Identities = 21/56 (37%), Positives = 31/56 (54%)
Frame = +2
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFIFQSAS 141
GRLAMIGF A + VE+A G+GL + +P L + L+ + + + FQ S
Sbjct: 437 GRLAMIGFVAAMGVEIAKGQGLFDQIS-GGGIPWF-LGTSVLLSLASLIPFFQGVS 598
Score = 26.9 bits (58), Expect = 2.8
Identities = 13/42 (30%), Positives = 21/42 (49%)
Frame = +2
Query: 67 VSIRSEQSTKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLL 108
VS+ S+ + ++W GR+AM+G A E G L+
Sbjct: 593 VSVESKSKGVMSSDAELWNGRIAMLGLIALAFTEYVKGTSLV 718
>TC76979 homologue to GP|14487954|gb|AAK63815.1 early light inducible
protein {Medicago sativa}, partial (93%)
Length = 787
Score = 35.8 bits (81), Expect = 0.006
Identities = 21/56 (37%), Positives = 31/56 (54%)
Frame = +1
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFIFQSAS 141
GRLAMIGF A + VE+A G+GL + +P L + L+ + + + FQ S
Sbjct: 427 GRLAMIGFVAAMGVEIAKGQGLFDQIS-GGGIPWF-LGTSVLLSLASLIPFFQGVS 588
Score = 26.9 bits (58), Expect = 2.8
Identities = 13/42 (30%), Positives = 21/42 (49%)
Frame = +1
Query: 67 VSIRSEQSTKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLL 108
VS+ S+ + ++W GR+AM+G A E G L+
Sbjct: 583 VSVESKSKGVMSSDAELWNGRIAMLGLIALAFTEYVKGTSLV 708
>TC76980 similar to GP|14487954|gb|AAK63815.1 early light inducible protein
{Medicago sativa}, partial (87%)
Length = 833
Score = 34.7 bits (78), Expect = 0.013
Identities = 25/61 (40%), Positives = 35/61 (56%), Gaps = 4/61 (6%)
Frame = +2
Query: 86 GRLAMIGFAAGITVEVATGKGLLENF-GVASP---LPTVALAVTALVGVLTAVFIFQSAS 141
GRLAMIGF + + VE+A G+GL + G P +V L V +LV + V + +S S
Sbjct: 371 GRLAMIGFVSAMGVELAKGQGLFDQISGGGIPWFLYTSVLLTVASLVPLFEGVSV-ESKS 547
Query: 142 K 142
K
Sbjct: 548 K 550
Score = 27.7 bits (60), Expect = 1.6
Identities = 13/42 (30%), Positives = 21/42 (49%)
Frame = +2
Query: 67 VSIRSEQSTKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLL 108
VS+ S+ + ++W GR+AM+G A E G L+
Sbjct: 527 VSVESKSKGIMSSNAELWNGRIAMLGLIALAVTEYVKGTALV 652
>TC90438 weakly similar to GP|21592719|gb|AAM64668.1 unknown {Arabidopsis
thaliana}, partial (70%)
Length = 689
Score = 33.1 bits (74), Expect = 0.039
Identities = 16/59 (27%), Positives = 33/59 (55%)
Frame = +1
Query: 50 LLIRRSHGRKLACRAMPVSIRSEQSTKDGNGLDVWLGRLAMIGFAAGITVEVATGKGLL 108
+++ ++LA + M + I++++ K ++ G AMIG AG+ +E TGK ++
Sbjct: 244 IMVDPVEAKRLAAKQMEI-IKAKEKLKKRRQIEAINGAWAMIGLTAGLVIEGQTGKNII 417
>TC90281 similar to SP|P54225|HEMZ_SYNY3 Ferrochelatase (EC 4.99.1.1)
(Protoheme ferro-lyase) (Heme synthetase). [strain PCC
6803], partial (4%)
Length = 1079
Score = 32.3 bits (72), Expect = 0.066
Identities = 17/48 (35%), Positives = 25/48 (51%)
Frame = +2
Query: 87 RLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAV 134
RLAMIG G ++ TGK L+ + A+PLP + V + L +
Sbjct: 395 RLAMIGVTTGYLTKLITGKNTLDQYS-AAPLPVILTFVLFIAASLIPI 535
>TC86769 similar to GP|14190393|gb|AAK55677.1 At1g34000/F12G12_210
{Arabidopsis thaliana}, partial (48%)
Length = 860
Score = 32.3 bits (72), Expect = 0.066
Identities = 18/35 (51%), Positives = 20/35 (56%), Gaps = 7/35 (20%)
Frame = +1
Query: 86 GRLAMIGFAAGITVEVATG-------KGLLENFGV 113
GR AM GFA G+ E ATG K LL NFG+
Sbjct: 562 GRWAMFGFAVGLLTEFATGSDFVDQVKILLSNFGI 666
>TC79896 similar to GP|15795154|dbj|BAB03142. gene_id:MQC3.16~unknown
protein {Arabidopsis thaliana}, partial (58%)
Length = 739
Score = 30.4 bits (67), Expect = 0.25
Identities = 12/23 (52%), Positives = 19/23 (82%)
Frame = +1
Query: 86 GRLAMIGFAAGITVEVATGKGLL 108
GR+A++G A + VE+ATGKG++
Sbjct: 409 GRIALLGLTALLLVELATGKGVI 477
>AW774435 homologue to GP|20146255|dbj hypothetical protein {Oryza sativa
(japonica cultivar-group)}, partial (20%)
Length = 594
Score = 30.0 bits (66), Expect = 0.33
Identities = 17/34 (50%), Positives = 19/34 (55%), Gaps = 7/34 (20%)
Frame = -1
Query: 87 RLAMIGFAAGITVEVATG-------KGLLENFGV 113
R AM GFA G+ E ATG K LL NFG+
Sbjct: 237 RWAMFGFAVGLLTEFATGSDFVDQVKILLSNFGI 136
>AW774436 homologue to GP|20146255|dbj hypothetical protein {Oryza sativa
(japonica cultivar-group)}, partial (20%)
Length = 671
Score = 30.0 bits (66), Expect = 0.33
Identities = 17/34 (50%), Positives = 19/34 (55%), Gaps = 7/34 (20%)
Frame = -1
Query: 87 RLAMIGFAAGITVEVATG-------KGLLENFGV 113
R AM GFA G+ E ATG K LL NFG+
Sbjct: 239 RWAMFGFAVGLLTEFATGSDFVDQVKILLSNFGI 138
>TC88385 similar to GP|22136512|gb|AAM91334.1 unknown protein {Arabidopsis
thaliana}, partial (71%)
Length = 1001
Score = 28.5 bits (62), Expect = 0.96
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = +2
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFG 112
G+ A+IGF + E+ TGKGLL+ G
Sbjct: 749 GKAAVIGFLLLLDFELLTGKGLLKGTG 829
>BQ144045 similar to PIR|T51947|T51 probable transcription factor HUA2
[imported] - Arabidopsis thaliana, partial (1%)
Length = 1172
Score = 27.7 bits (60), Expect = 1.6
Identities = 22/68 (32%), Positives = 30/68 (43%), Gaps = 8/68 (11%)
Frame = -2
Query: 9 ASLSISFRDACVFP-------AAKYSATAR-FPLPNLVPSRASFATGTPLLIRRSHGRKL 60
ASLS+ CV AA Y ++R P P L+P RA + +P +R S R
Sbjct: 904 ASLSLDDERRCVISIFRGEAAAACYLTSSRNIPAPGLIPLRALSSRISPFCMRISRQRAY 725
Query: 61 ACRAMPVS 68
P+S
Sbjct: 724 TTLVRPLS 701
>BG450637 similar to SP|P14895|ELI5_ High molecular mass early
light-inducible protein HV58 chloroplast precursor
(ELIP). [Barley], partial (10%)
Length = 678
Score = 27.3 bits (59), Expect = 2.1
Identities = 18/43 (41%), Positives = 24/43 (54%)
Frame = +2
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALV 128
GRLAM+GF G E TG LL+ A +P + LA+ L+
Sbjct: 482 GRLAMLGFLLGTIGEFRTGTPLLQQ---AQAIP-IQLAILFLL 598
>BQ750994 similar to GP|15408680|db putative multi resistance protein ABC
transporter {Oryza sativa (japonica cultivar-group)},
partial (1%)
Length = 577
Score = 26.6 bits (57), Expect = 3.6
Identities = 21/83 (25%), Positives = 34/83 (40%), Gaps = 4/83 (4%)
Frame = -3
Query: 19 CVF-PAAKYSATARFPLPNLVPS---RASFATGTPLLIRRSHGRKLACRAMPVSIRSEQS 74
C F P+++ +A P P L+ R +T I GR+++CR P + S S
Sbjct: 488 CTFSPSSRSRTSASLPTPRLMRPQTLRRR*STNPVSAIGSEVGRRISCRTRPATSPSRLS 309
Query: 75 TKDGNGLDVWLGRLAMIGFAAGI 97
+ G LA +A +
Sbjct: 308 RRRDGGCPAG*AGLARAASSAAL 240
>TC77155 homologue to GP|15028015|gb|AAK76538.1 unknown protein {Arabidopsis
thaliana}, partial (98%)
Length = 1099
Score = 26.6 bits (57), Expect = 3.6
Identities = 17/49 (34%), Positives = 26/49 (52%)
Frame = -3
Query: 3 LATQTSASLSISFRDACVFPAAKYSATARFPLPNLVPSRASFATGTPLL 51
L T + S SF+ + P ++A+ RFPLPN+ + F + PLL
Sbjct: 287 LKKSTVLTASHSFKSS---PGGSFTASLRFPLPNV--ASMCFLS*APLL 156
>TC87694 similar to PIR|T48233|T48233 one helix protein (OHP) - Arabidopsis
thaliana, partial (67%)
Length = 688
Score = 26.6 bits (57), Expect = 3.6
Identities = 11/32 (34%), Positives = 17/32 (52%)
Frame = +3
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGV 113
++W R M+G VE+ KG+LE G+
Sbjct: 357 EIWNSRACMMGLIGTFIVELIIHKGILEVIGL 452
>TC76410 similar to GP|4689382|gb|AAD27878.1| chlorophyll a/b binding
protein CP29 {Vigna radiata}, complete
Length = 1138
Score = 26.6 bits (57), Expect = 3.6
Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 2/38 (5%)
Frame = +2
Query: 87 RLAMIGFAAGITVEVATGKGLLENFG--VASPLPTVAL 122
RLAM+ F ATGKG L N+ ++ PL T +
Sbjct: 779 RLAMVAFLGFAVQAAATGKGPLNNWATHLSDPLHTTII 892
>TC76507 homologue to GP|506629|gb|AAA50172.1|| photosystem II type I
chlorophyll a/b-binding protein {Glycine max}, complete
Length = 1029
Score = 26.2 bits (56), Expect = 4.7
Identities = 16/40 (40%), Positives = 19/40 (47%), Gaps = 2/40 (5%)
Frame = +1
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFG--VASPLPTVALA 123
GRLAM + TGKG LEN +A P+ A A
Sbjct: 730 GRLAMFSMFGFFVQAIVTGKGPLENLADHIADPVNNNAWA 849
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.133 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,523,968
Number of Sequences: 36976
Number of extensions: 40167
Number of successful extensions: 239
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 233
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 238
length of query: 143
length of database: 9,014,727
effective HSP length: 87
effective length of query: 56
effective length of database: 5,797,815
effective search space: 324677640
effective search space used: 324677640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0290.12