Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0278.5.1
         (30 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC88481 similar to PIR|T00806|T02445 probable U4/U6 small nuclea...    44  1e-05
TC88235 similar to GP|12656803|gb|AAK00964.1 unknown protein {Or...    27  2.4
TC85659 SP|O24076|GBLP_MEDSA Guanine nucleotide-binding protein ...    26  4.0
BF004489 similar to GP|20161344|d contains ESTs AU086067(S4642) ...    25  6.9
TC90611 similar to GP|10177223|dbj|BAB10298. contains similarity...    25  6.9
TC78016 similar to GP|21537191|gb|AAM61532.1 PRL1 protein {Arabi...    25  6.9
TC78679 similar to GP|20466538|gb|AAM20586.1 putative WD-repeat ...    25  6.9
TC87555 weakly similar to GP|22775656|dbj|BAC15510. contains EST...    25  6.9
TC92589 similar to GP|11141605|gb|AAG32022.1 LEUNIG {Arabidopsis...    25  6.9
TC79260 weakly similar to GP|9759350|dbj|BAB10005.1 WD-repeat pr...    25  9.0

>TC88481 similar to PIR|T00806|T02445 probable U4/U6 small nuclear
            ribonucleoprotein [imported] - Arabidopsis thaliana,
            partial (60%)
          Length = 1836

 Score = 44.3 bits (103), Expect = 1e-05
 Identities = 22/30 (73%), Positives = 22/30 (73%)
 Frame = +1

Query: 1    GGYIVTVSHDRTIKLWSSNMTNDNEHTMDV 30
            GGYIVTVSHDRTIKLWS   T    H MDV
Sbjct: 1744 GGYIVTVSHDRTIKLWSXXTT--XXHAMDV 1827


>TC88235 similar to GP|12656803|gb|AAK00964.1 unknown protein {Oryza sativa},
            partial (91%)
          Length = 1080

 Score = 26.6 bits (57), Expect = 2.4
 Identities = 9/18 (50%), Positives = 13/18 (72%)
 Frame = +2

Query: 1    GGYIVTVSHDRTIKLWSS 18
            G Y++T S D T +LWS+
Sbjct: 1016 GAYLITASSDSTARLWST 1069


>TC85659 SP|O24076|GBLP_MEDSA Guanine nucleotide-binding protein beta
           subunit-like protein. [Alfalfa] {Medicago sativa},
           complete
          Length = 1261

 Score = 25.8 bits (55), Expect = 4.0
 Identities = 10/15 (66%), Positives = 13/15 (86%)
 Frame = +2

Query: 4   IVTVSHDRTIKLWSS 18
           IV+ S DRTIKLW++
Sbjct: 419 IVSASRDRTIKLWNT 463



 Score = 25.0 bits (53), Expect = 6.9
 Identities = 11/19 (57%), Positives = 15/19 (78%)
 Frame = +2

Query: 4   IVTVSHDRTIKLWSSNMTN 22
           IV+ S DRT+K+W  N+TN
Sbjct: 557 IVSASWDRTVKVW--NLTN 607


>BF004489 similar to GP|20161344|d contains ESTs AU086067(S4642)
           D41819(S4642)~similar to Arabidopsis thaliana chromosome
           1  At1g49040~, partial (16%)
          Length = 605

 Score = 25.0 bits (53), Expect = 6.9
 Identities = 8/16 (50%), Positives = 12/16 (75%)
 Frame = +1

Query: 1   GGYIVTVSHDRTIKLW 16
           G  ++T SHD T+K+W
Sbjct: 178 GERVLTASHDGTVKMW 225


>TC90611 similar to GP|10177223|dbj|BAB10298. contains similarity to
           GTP-binding regulatory protein and WD-repeat
           protein~gene_id:MPF21.14, partial (40%)
          Length = 855

 Score = 25.0 bits (53), Expect = 6.9
 Identities = 9/16 (56%), Positives = 13/16 (81%)
 Frame = +1

Query: 1   GGYIVTVSHDRTIKLW 16
           G ++ +VS DRTIK+W
Sbjct: 517 GKFLYSVSWDRTIKIW 564


>TC78016 similar to GP|21537191|gb|AAM61532.1 PRL1 protein {Arabidopsis
            thaliana}, partial (81%)
          Length = 1759

 Score = 25.0 bits (53), Expect = 6.9
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = +3

Query: 4    IVTVSHDRTIKLW 16
            +VT SHD TIK+W
Sbjct: 1011 VVTGSHDSTIKMW 1049


>TC78679 similar to GP|20466538|gb|AAM20586.1 putative WD-repeat protein
           {Arabidopsis thaliana}, partial (55%)
          Length = 1198

 Score = 25.0 bits (53), Expect = 6.9
 Identities = 12/28 (42%), Positives = 18/28 (63%), Gaps = 5/28 (17%)
 Frame = +1

Query: 1   GGYIVTVSHDRTIKLW-----SSNMTND 23
           G Y ++   D+TIKLW     SSN+T++
Sbjct: 487 GRYFISNGKDQTIKLWDIRKMSSNVTSN 570


>TC87555 weakly similar to GP|22775656|dbj|BAC15510. contains EST
           C27990(C53630)~similar to G-protein beta family {Oryza
           sativa (japonica cultivar-group), partial (33%)
          Length = 685

 Score = 25.0 bits (53), Expect = 6.9
 Identities = 9/23 (39%), Positives = 12/23 (52%)
 Frame = +1

Query: 4   IVTVSHDRTIKLWSSNMTNDNEH 26
           + T S DRT++LW  N      H
Sbjct: 583 LATASKDRTLRLWKINTEEATNH 651


>TC92589 similar to GP|11141605|gb|AAG32022.1 LEUNIG {Arabidopsis thaliana},
           partial (5%)
          Length = 415

 Score = 25.0 bits (53), Expect = 6.9
 Identities = 8/15 (53%), Positives = 11/15 (73%)
 Frame = +1

Query: 2   GYIVTVSHDRTIKLW 16
           G + + SHDR +KLW
Sbjct: 103 GLVASASHDRFVKLW 147


>TC79260 weakly similar to GP|9759350|dbj|BAB10005.1 WD-repeat protein-like
           {Arabidopsis thaliana}, partial (50%)
          Length = 1227

 Score = 24.6 bits (52), Expect = 9.0
 Identities = 11/23 (47%), Positives = 15/23 (64%)
 Frame = +2

Query: 1   GGYIVTVSHDRTIKLWSSNMTND 23
           G Y+ T S+DRT  +W  + TND
Sbjct: 581 GKYLATASNDRTAIIWEVD-TND 646


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.313    0.128    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,033,420
Number of Sequences: 36976
Number of extensions: 4781
Number of successful extensions: 65
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 58
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 65
length of query: 30
length of database: 9,014,727
effective HSP length: 6
effective length of query: 24
effective length of database: 8,792,871
effective search space: 211028904
effective search space used: 211028904
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)


Lotus: description of TM0278.5.1