BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0276.3
(333 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79001 similar to GP|16649093|gb|AAL24398.1 proline iminopeptid... 650 0.0
TC88153 similar to GP|1354849|gb|AAB02006.1| epoxide hydrolase {... 48 5e-06
TC78414 similar to GP|1354849|gb|AAB02006.1| epoxide hydrolase {... 43 2e-04
TC89015 similar to PIR|T47946|T47946 prolyl aminopeptidase-like ... 42 3e-04
TC79420 weakly similar to GP|1354849|gb|AAB02006.1| epoxide hydr... 40 0.002
TC88515 weakly similar to PIR|T45731|T45731 epoxide hydrolase-li... 38 0.005
TC79861 similar to PIR|T01316|T01316 epoxide hydrolase homolog T... 37 0.011
TC80511 similar to GP|8809594|dbj|BAA97145.1 gene_id:MNB8.11~unk... 36 0.024
TC77524 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 33 0.12
TC77584 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 33 0.15
TC77583 similar to PIR|T07145|T07145 epoxide hydrolase homolog -... 32 0.26
AW690141 homologue to GP|14582465|gb| putative transcription fac... 32 0.45
BF640605 similar to GP|20259141|gb unknown protein {Arabidopsis ... 32 0.45
BF519064 similar to PIR|S57483|S574 glycin-rich protein - cowpea... 30 0.99
TC87411 similar to GP|20259141|gb|AAM14286.1 unknown protein {Ar... 30 1.3
TC91905 similar to GP|19347881|gb|AAL85997.1 unknown protein {Ar... 30 1.7
TC81324 similar to GP|21553871|gb|AAM62964.1 unknown {Arabidopsi... 28 3.8
TC88582 similar to GP|9759314|dbj|BAB09681.1 splicing factor 3a ... 28 3.8
>TC79001 similar to GP|16649093|gb|AAL24398.1 proline iminopeptidase
{Arabidopsis thaliana}, partial (78%)
Length = 1534
Score = 650 bits (1676), Expect = 0.0
Identities = 305/331 (92%), Positives = 321/331 (96%), Gaps = 1/331 (0%)
Frame = +1
Query: 1 MASE-QEFSELNRNLYPHIEPYSTGLLKVSDLHTIYWEQSGNPSGHPVVFLHGGPGGGTS 59
MAS+ +E S +N NLYP IEPY+TG LKVSDLHTIYWEQSGNP+GHPVVFLHGGPGGGTS
Sbjct: 178 MASQKEEISPVNTNLYPDIEPYTTGFLKVSDLHTIYWEQSGNPTGHPVVFLHGGPGGGTS 357
Query: 60 PSNRRFFDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQVFGG 119
PSNRRFFDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHL+IPEWQVFGG
Sbjct: 358 PSNRRFFDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLQIPEWQVFGG 537
Query: 120 SWGSTLALAYSQSHPDKVTGIILRGIFLLRKKEIDWFYEGGAAAIFPDAWEPFRDLIPEN 179
SWGSTLALAYSQSHPDKVTGI+LRGIFLLRKKEIDWFYEGGAAAI+PDAWEPFRDLIPE
Sbjct: 538 SWGSTLALAYSQSHPDKVTGIVLRGIFLLRKKEIDWFYEGGAAAIYPDAWEPFRDLIPEK 717
Query: 180 ERGCFVDAYKKRLNSDDIQTQFAAARAWTKWEMMTAHLIPNEDNVKKGDDDHFSLAFARI 239
ERGCF+DAYKKRLNSDDI+TQ+AAARAWTKWEMMTAHL+PNED VK+GDDD+FSLAFARI
Sbjct: 718 ERGCFIDAYKKRLNSDDIKTQYAAARAWTKWEMMTAHLLPNEDMVKRGDDDYFSLAFARI 897
Query: 240 ENHYFVNKGFFSSDSYLLDGVDKIRHINTTIVQGRYDVCCPMMSAWDLHKAWPEADFRVV 299
ENHYFVNKGFFSSDS+LLDGVDKIRHINTTIVQGRYDVCCPMMSAWDLHKAWPEADFRVV
Sbjct: 898 ENHYFVNKGFFSSDSFLLDGVDKIRHINTTIVQGRYDVCCPMMSAWDLHKAWPEADFRVV 1077
Query: 300 ADAGHSANELGIAAELVAANEKLKNIIKNKG 330
+DAGHSANE GIAAELVAANEKLKN IKNKG
Sbjct: 1078SDAGHSANEPGIAAELVAANEKLKNKIKNKG 1170
>TC88153 similar to GP|1354849|gb|AAB02006.1| epoxide hydrolase {Nicotiana
tabacum}, partial (82%)
Length = 1143
Score = 48.1 bits (113), Expect = 5e-06
Identities = 28/75 (37%), Positives = 39/75 (51%)
Frame = +1
Query: 71 YRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYS 130
YR I FD RG G S A E T DL+DD++ L + L I + G +G+ A +
Sbjct: 178 YRAIAFDFRGYGLSDHPAEPEKATIMDLVDDVKDLLDTLGISNAILIGKDFGAIPAYLVA 357
Query: 131 QSHPDKVTGIILRGI 145
HP+KV +I G+
Sbjct: 358 AVHPEKVASVITLGV 402
>TC78414 similar to GP|1354849|gb|AAB02006.1| epoxide hydrolase {Nicotiana
tabacum}, partial (68%)
Length = 900
Score = 42.7 bits (99), Expect = 2e-04
Identities = 31/100 (31%), Positives = 49/100 (49%), Gaps = 1/100 (1%)
Frame = +2
Query: 47 VVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKL 105
VVFLHG P S ++ +R I FD RG G S E T L++D+ +
Sbjct: 167 VVFLHGFPEIWYSWRHQMIAVAGAGFRAIAFDYRGYGLSDSPPEPEKTTFTHLLNDLLAI 346
Query: 106 REHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI 145
+ L + + + G +G+ A +S HP++V G+I G+
Sbjct: 347 LDALSLSKVFLVGKDFGARPAYLFSILHPERVLGVITLGV 466
>TC89015 similar to PIR|T47946|T47946 prolyl aminopeptidase-like protein -
Arabidopsis thaliana, partial (48%)
Length = 896
Score = 42.0 bits (97), Expect = 3e-04
Identities = 37/120 (30%), Positives = 51/120 (41%), Gaps = 22/120 (18%)
Frame = +2
Query: 47 VVFLHGGPGGGTSPSNRRFF----DPEFYRIILFDQRGAGKSTP---HACLEHNTTWDLI 99
++FL GGPG P E +R+IL DQRG G STP + + + DL
Sbjct: 293 LLFLQGGPGFECRPPTESSGWIQKVCEQFRLILMDQRGTGLSTPLSVSSMSQFKSAQDLA 472
Query: 100 D------------DIEKLREHLEIPE---WQVFGGSWGSTLALAYSQSHPDKVTGIILRG 144
D D E +R L +P W + G S+G A+ Y P +T +L G
Sbjct: 473 DFLKYFRADSIVKDAEFIRVRL-VPNAGPWTILGQSYGGFCAVTYLSFAPQGLTQALLTG 649
>TC79420 weakly similar to GP|1354849|gb|AAB02006.1| epoxide hydrolase
{Nicotiana tabacum}, partial (90%)
Length = 1433
Score = 39.7 bits (91), Expect = 0.002
Identities = 31/100 (31%), Positives = 48/100 (48%), Gaps = 1/100 (1%)
Frame = +2
Query: 47 VVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKL 105
VVFLHG P S ++ +R I D RG G S E T L++D+ ++
Sbjct: 413 VVFLHGFPEIWYSWRHQMLALAGAGFRAIAPDYRGYGLSDSPPEPEKTTFTHLLNDLLQI 592
Query: 106 REHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI 145
+ L I + + G +G A +S HP++V G+I G+
Sbjct: 593 IDALAISKVFLVGKDFGGPPAYLFSILHPERVLGVITLGV 712
>TC88515 weakly similar to PIR|T45731|T45731 epoxide hydrolase-like protein
- Arabidopsis thaliana, partial (51%)
Length = 771
Score = 38.1 bits (87), Expect = 0.005
Identities = 27/104 (25%), Positives = 44/104 (41%), Gaps = 1/104 (0%)
Frame = +2
Query: 43 SGHPVVFLHGGPGGGTSPSNR-RFFDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDD 101
+G V+ LHG P S ++ + Y + D RG G S + T ++ D
Sbjct: 215 TGPLVLLLHGFPEIWYSWRHQLNYLAQNGYHAVAPDLRGYGDSDSPINSDSYTLHHIVGD 394
Query: 102 IEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI 145
+ L +H + V G WG+ + S PD+V G + G+
Sbjct: 395 LIGLLDHFGEHKAYVVGSDWGANIGWHLSLFRPDRVKGFVALGV 526
>TC79861 similar to PIR|T01316|T01316 epoxide hydrolase homolog T14P8.15 -
Arabidopsis thaliana, partial (86%)
Length = 1162
Score = 37.0 bits (84), Expect = 0.011
Identities = 25/100 (25%), Positives = 44/100 (44%), Gaps = 1/100 (1%)
Frame = +1
Query: 43 SGHPVVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDD 101
SG ++FLHG P S ++ YR + D RG G + + T ++ D
Sbjct: 79 SGPVIIFLHGFPELWYSWRHQLLSLSALGYRAVAPDLRGYGDTDSPSSPSSYTAHHIVGD 258
Query: 102 IEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGII 141
+ L + L + + G WG+ +A + PD+V ++
Sbjct: 259 LVGLLDALNVDRVFLVGHDWGAAMAWYFCLLKPDRVKALV 378
>TC80511 similar to GP|8809594|dbj|BAA97145.1 gene_id:MNB8.11~unknown
protein {Arabidopsis thaliana}, partial (47%)
Length = 905
Score = 35.8 bits (81), Expect = 0.024
Identities = 22/71 (30%), Positives = 34/71 (46%)
Frame = +1
Query: 72 RIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQ 131
++ FD RG G+S+ +T + D L +HL + VFG S GS +A +
Sbjct: 397 QVCAFDNRGVGRSSVPIRKSDYSTKIMAKDAITLLDHLGWKKAHVFGHSMGSMIACKLAA 576
Query: 132 SHPDKVTGIIL 142
PD+V + L
Sbjct: 577 MVPDRVLSMAL 609
>TC77524 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (81%)
Length = 1262
Score = 33.5 bits (75), Expect = 0.12
Identities = 32/129 (24%), Positives = 53/129 (40%), Gaps = 5/129 (3%)
Frame = +2
Query: 44 GHPVVFLHGGPGGGTSPSNR-RFFDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ YR + D RG G + A + T + L+ DI
Sbjct: 86 GPVVLFLHGFPELWYSWRHQIAALGSLGYRAVAPDLRGYGDTDAPASISSYTIFHLVGDI 265
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGIFLL----RKKEIDWFYE 158
L + L + + + WG+ + P+K+ + + L + K +D
Sbjct: 266 VALIDSLGVDQVFLVAHDWGAMIGWYLCLFRPEKIKAYVCLSVPYLPRNPKMKPVD---- 433
Query: 159 GGAAAIFPD 167
G A+F D
Sbjct: 434 -GMRALFGD 457
>TC77584 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (70%)
Length = 819
Score = 33.1 bits (74), Expect = 0.15
Identities = 24/108 (22%), Positives = 47/108 (43%), Gaps = 1/108 (0%)
Frame = +2
Query: 44 GHPVVFLHGGPGGGTSPSNRRFFDPEF-YRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ YR + D RG G + + + T + ++ D+
Sbjct: 149 GPVVLFLHGFPELWYSWRHQIVALSSLGYRAVAPDLRGYGDTDAPSSVSSYTGFHIVGDL 328
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGIFLLRK 150
L + L + + + WG+ + P+++ + + LLR+
Sbjct: 329 VALIDFLGVDQVFLVAHDWGAIIGWYLCMFRPERIKAYVCLSVPLLRR 472
>TC77583 similar to PIR|T07145|T07145 epoxide hydrolase homolog - soybean,
partial (92%)
Length = 1188
Score = 32.3 bits (72), Expect = 0.26
Identities = 24/108 (22%), Positives = 47/108 (43%), Gaps = 1/108 (0%)
Frame = +3
Query: 44 GHPVVFLHGGPGGGTSPSNRRFFDPEF-YRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ YR + D RG G + + + T + ++ D+
Sbjct: 114 GPVVLFLHGFPELWYSWRHQIVALGSLGYRAVAPDLRGYGDTEAPSSISSYTGFHIVGDL 293
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGIFLLRK 150
L + L + + + WG+ + P+++ + + LLR+
Sbjct: 294 VALIDLLGVDQVFLVAHDWGAIIGWYLCMFRPERIKAYVCLSVPLLRR 437
>AW690141 homologue to GP|14582465|gb| putative transcription factor {Vitis
vinifera}, partial (18%)
Length = 394
Score = 31.6 bits (70), Expect = 0.45
Identities = 15/37 (40%), Positives = 20/37 (53%)
Frame = -3
Query: 24 GLLKVSDLHTIYWEQSGNPSGHPVVFLHGGPGGGTSP 60
G V D+ +++ Q P H +LHGG GGG SP
Sbjct: 167 GAQDVCDVSPLFYSQ---PLQHSQTYLHGGNGGGASP 66
>BF640605 similar to GP|20259141|gb unknown protein {Arabidopsis thaliana},
partial (68%)
Length = 661
Score = 31.6 bits (70), Expect = 0.45
Identities = 30/120 (25%), Positives = 51/120 (42%), Gaps = 5/120 (4%)
Frame = +3
Query: 28 VSDLHTIYWEQSGNPSGHPVVFLHGGPGGGTSPSNRRFFDP---EFYRIILFDQRGAGKS 84
V + H + +GN +V HG GT S + F P + +R++L+D GAG +
Sbjct: 90 VEEAHNVKVLGTGN---RYIVLAHGF---GTDQSVWKHFVPYLVDDFRVVLYDNMGAGTT 251
Query: 85 TPH--ACLEHNTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIIL 142
P H++ D+ + E L+I G S + + S + PD +I+
Sbjct: 252 NPEYFDSERHSSLEGYAYDLLAILEELQIDSCIFVGHSVSAMIGAIASITRPDLFLKLIM 431
>BF519064 similar to PIR|S57483|S574 glycin-rich protein - cowpea (fragment),
partial (7%)
Length = 452
Score = 30.4 bits (67), Expect = 0.99
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = -3
Query: 134 PDKVTGIILRGIFLLRKKEIDWFYEGGAAAIFP 166
P G RG F +KK++ +F++GG FP
Sbjct: 297 PSPQKGFPKRGFFFNKKKKVFFFFKGGGGVFFP 199
>TC87411 similar to GP|20259141|gb|AAM14286.1 unknown protein {Arabidopsis
thaliana}, complete
Length = 1275
Score = 30.0 bits (66), Expect = 1.3
Identities = 26/93 (27%), Positives = 38/93 (39%), Gaps = 5/93 (5%)
Frame = +3
Query: 55 GGGTSPSNRRFFDPEF---YRIILFDQRGAGKSTP--HACLEHNTTWDLIDDIEKLREHL 109
G GT S + P +R+IL+D GAG + P ++T D+ + E L
Sbjct: 213 GFGTDQSVWKHLVPHLLDEFRVILYDNMGAGTTNPDYFDFERYSTLEGYAYDLLAILEEL 392
Query: 110 EIPEWQVFGGSWGSTLALAYSQSHPDKVTGIIL 142
+ G S + + S S PD IIL
Sbjct: 393 RVDSCIFVGHSVSAMIGTVASISRPDLFNKIIL 491
>TC91905 similar to GP|19347881|gb|AAL85997.1 unknown protein {Arabidopsis
thaliana}, partial (67%)
Length = 867
Score = 29.6 bits (65), Expect = 1.7
Identities = 13/41 (31%), Positives = 22/41 (52%)
Frame = +1
Query: 106 REHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGIF 146
R ++ VFG S G + ++++P+KV G+IL F
Sbjct: 670 RSDIDTSRIVVFGRSLGGAVGAVLTRNNPEKVAGLILENTF 792
>TC81324 similar to GP|21553871|gb|AAM62964.1 unknown {Arabidopsis
thaliana}, partial (75%)
Length = 1156
Score = 28.5 bits (62), Expect = 3.8
Identities = 18/51 (35%), Positives = 23/51 (44%)
Frame = +1
Query: 18 IEPYSTGLLKVSDLHTIYWEQSGNPSGHPVVFLHGGPGGGTSPSNRRFFDP 68
+E STG+ S T ++ SG P G FLH PGG + S P
Sbjct: 166 MESLSTGVGSKSKPCTKFFSTSGCPFGESCHFLHHVPGGYNAVSQMMNLTP 318
>TC88582 similar to GP|9759314|dbj|BAB09681.1 splicing factor 3a
{Arabidopsis thaliana}, partial (57%)
Length = 1184
Score = 28.5 bits (62), Expect = 3.8
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 2/70 (2%)
Frame = +3
Query: 138 TGIILRGIFLLRKKEIDWFYEGGAAAIFPDAWEPFRDLIPENERGCF--VDAYKKRLNSD 195
TGI L F R KEI ++ AA DA + F DL+ E + F +A+ + L+ +
Sbjct: 378 TGINLFSAFYDRLKEIREYHRKHPAARVVDANDDFEDLLKEEPKIEFSGEEAFGRYLDIN 557
Query: 196 DIQTQFAAAR 205
++ Q+ ++
Sbjct: 558 ELYQQYVNSK 587
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,243,873
Number of Sequences: 36976
Number of extensions: 164343
Number of successful extensions: 671
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 667
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 670
length of query: 333
length of database: 9,014,727
effective HSP length: 97
effective length of query: 236
effective length of database: 5,428,055
effective search space: 1281020980
effective search space used: 1281020980
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0276.3