BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0269b.9
(1085 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86534 homologue to SP|P12227|RPOD_PEA DNA-directed RNA polymer... 708 0.0
BI310662 homologue to SP|P12227|RPO DNA-directed RNA polymerase ... 181 2e-45
TC82897 similar to GP|16604396|gb|AAL24204.1 AT5g18630/T1A4_10 {... 32 1.0
BE249090 30 3.9
TC80669 homologue to PIR|S53498|S53498 dnaK-type molecular chape... 30 6.6
TC77420 weakly similar to GP|19310745|gb|AAL85103.1 unknown prot... 30 6.6
AL389085 29 8.6
BE317600 29 8.6
TC77696 similar to GP|2673912|gb|AAB88646.1| expressed protein {... 29 8.6
TC77270 similar to GP|16973465|gb|AAL32300.1 lethal leaf spot 1-... 29 8.6
TC88358 proteinase inhibitor 20 [Medicago truncatula] 29 8.6
>TC86534 homologue to SP|P12227|RPOD_PEA DNA-directed RNA polymerase beta'
chain (EC 2.7.7.6) (Fragment). [Garden pea] {Pisum
sativum}, partial (61%)
Length = 2441
Score = 708 bits (1827), Expect(2) = 0.0
Identities = 364/513 (70%), Positives = 411/513 (79%), Gaps = 35/513 (6%)
Frame = +1
Query: 159 IYIGSRCIVVRNQDIGIGLINRFINFQTQPIFIRTPFTCRNTSWICRLCYGRSPIHGDLV 218
IYIGSRCIVVRNQDIGIGLINRFI FQTQPIFIRTPFTCRNTSWICRLCYGRSPIHGDLV
Sbjct: 421 IYIGSRCIVVRNQDIGIGLINRFITFQTQPIFIRTPFTCRNTSWICRLCYGRSPIHGDLV 600
Query: 219 ELGEAVGIIAGQSIGEPGTQLTLITFHTGGVFTGGTAEYVRSPSNGKIKFNENSAYPTRT 278
ELGEAVGIIAGQSIGEPGTQLTL TFHTGGVFTGGTAEY R+PSNGKIKFNE+ +PTRT
Sbjct: 601 ELGEAVGIIAGQSIGEPGTQLTLRTFHTGGVFTGGTAEYARAPSNGKIKFNEDLVHPTRT 780
Query: 279 RHGHPAFLCYIDLYVTIESNDIMHNVIIPPKSFLLVQNDQYVKSEQIIAEIRAGTYTLNL 338
RHG+PAF+C IDLYVT+ES+DI+HNVIIPPKSFLLVQNDQYVKSEQ+IAEIRAGT T NL
Sbjct: 781 RHGYPAFICNIDLYVTVESDDIIHNVIIPPKSFLLVQNDQYVKSEQVIAEIRAGTSTFNL 960
Query: 339 KEKIRKHIFSDSEGEMHWSTNIYHVSEFAYSNVHILPKTSHLWILSGNSHKSDTVSLSLL 398
KE++RKHI+SDSEGEMHWST++YH SEF YSNVHILPKTSHLWILSG S +SDT+ L
Sbjct: 961 KERVRKHIYSDSEGEMHWSTDVYHASEFLYSNVHILPKTSHLWILSGKSCRSDTIHFLLR 1140
Query: 399 KDQDQMSTHSLPTAKRNTSNFLVSNNQVRL-------------------------CPDHC 433
KDQDQ++ SL T KRN SN LVSN++V+L C DH
Sbjct: 1141KDQDQINIDSLSTGKRNISNLLVSNDEVKLKLLSLKTFGTKEKGISNYSILNQMICTDHS 1320
Query: 434 HFMHPTISPDTSNLLAKKRRNRFIIPFLFRSIRERNNELMP--DISVEIPIDGIIHKNSI 491
H M+P I DT LLAK+RRNRF+IP F+SI+ER NELM +S+EIPI+GI H+NSI
Sbjct: 1321HLMYPAIFHDTFYLLAKRRRNRFLIP--FQSIQERKNELMRPFGVSIEIPINGIFHRNSI 1494
Query: 492 LAYFDDPQYRTQSSGIAKYKTIGIHSIFQKEDLIEYRGIREFKPKYQIKVDRFFFIPQEV 551
AYFDDPQYR Q+SGI KY+T+GIHS+FQKED IEYRGI+E KPKYQIKVDRFFFIP+EV
Sbjct: 1495FAYFDDPQYRRQNSGITKYRTVGIHSVFQKEDFIEYRGIKELKPKYQIKVDRFFFIPEEV 1674
Query: 552 HILSESSSIMVRNNSIIGVNTPITLNKKSRVGGLVRVEKNKKK-------IELKIF-SGD 603
HIL ESSSIMVRNNS++G+ TPIT N +SRVGGLVR+EK KKK +E+ IF
Sbjct: 1675HILPESSSIMVRNNSLVGIGTPITFNIRSRVGGLVRLEKKKKKKSN*NYFLEISIFPERG 1854
Query: 604 IHFPGEIDKISQHSAILIPPEMVKKKILRNQKK 636
I +P + +S + PE KK RNQKK
Sbjct: 1855IRYPDTV--LS*YH-----PER*KKN-GRNQKK 1929
Score = 323 bits (828), Expect = 2e-88
Identities = 170/225 (75%), Positives = 192/225 (84%), Gaps = 1/225 (0%)
Frame = +3
Query: 578 KKSRVGGLVRVEKNKKKIELKIFSGDIHFPGEIDKISQHSAILIPPEMVKKKILRNQKKK 637
K SR G +R EK KKKIELK+FSG+IHFPGE DKIS+HS+ILIPP VKKK + KK
Sbjct: 1758 KSSRRIGPIRKEK-KKKIELKLFSGNIHFPGERDKISRHSSILIPPGTVKKK-RKESKKI 1931
Query: 638 TNWRYIQWITTTKKKYFVLVRPVILYDIADSINLVKLFPQDLFKEWDNLELKVLNFILYG 697
NW Y+QWI TTKKKYFVLVRPVILY+I DSI+ +KLFPQDLF+E DNLELKV+N+ILYG
Sbjct: 1932 NNWIYVQWIATTKKKYFVLVRPVILYEIPDSIDFIKLFPQDLFQERDNLELKVVNYILYG 2111
Query: 698 NGKSIRGILDTSIQLVRTCLVLNWNEDEKSSSIEEALASFVEVSTNGLIRYFLRIDLVKS 757
NGKSIRGI DT IQLVRTCLVLNW++ +KS SIEEA +SFVEVSTNGLI YFLRIDLVKS
Sbjct: 2112 NGKSIRGISDTRIQLVRTCLVLNWDQGKKSPSIEEAPSSFVEVSTNGLIEYFLRIDLVKS 2291
Query: 758 HISYIRKRNDPSSSGLISYNESDRININPFFSI-YKENIQQSLSQ 801
+ +YIRKRNDPS GLI+ NESDRININPFFSI YK I++SLSQ
Sbjct: 2292 NTAYIRKRNDPSGLGLIADNESDRININPFFSIHYKAKIKKSLSQ 2426
Score = 234 bits (596), Expect(2) = 0.0
Identities = 117/141 (82%), Positives = 119/141 (83%)
Frame = +3
Query: 20 NESQF*YD*PL*SSPYNVFFGS*GKCISSAPISRYERINVGSPGPND*FTHSKQFT*RTF 79
+ES F* D*PL*SSPYNVFF S* KCISSAPISR ERINV S ND*FTHSKQFT RT
Sbjct: 6 SES*F*DD*PL*SSPYNVFFRS*RKCISSAPISRNERINVRSTRTND*FTHSKQFTGRTV 185
Query: 80 FNRIYYFLLWSPQGGCGYCCTNIRCWISYTETC*SGSTHCCTPNRLRYRSRDFCKYSE*N 139
FNRIY FLL SP GCGYCCTNIRCWISYT TC*SGSTHCCT NRLRY SRDFCKY + N
Sbjct: 186 FNRIYNFLLRSP*RGCGYCCTNIRCWISYTSTC*SGSTHCCTSNRLRYDSRDFCKYPKWN 365
Query: 140 DVRKNFDTNINWSCISRRYIY 160
D RKNFDTNINWSC SRRYIY
Sbjct: 366 DTRKNFDTNINWSCSSRRYIY 428
>BI310662 homologue to SP|P12227|RPO DNA-directed RNA polymerase beta' chain
(EC 2.7.7.6) (Fragment). [Garden pea] {Pisum sativum},
partial (19%)
Length = 688
Score = 181 bits (458), Expect = 2e-45
Identities = 94/120 (78%), Positives = 100/120 (83%), Gaps = 2/120 (1%)
Frame = +2
Query: 966 GPNLK--SGQVITVQMDFVGIRLANPYLATPGATIHGHYGEMLYEGDILVTFIYEKSRSG 1023
GP+LK SGQVITVQMD V IR ANPYLATP ATIHGHYGE+LY+GDILVTFIYEKSRS
Sbjct: 2 GPHLKLKSGQVITVQMDSVIIRSANPYLATPEATIHGHYGEILYQGDILVTFIYEKSRSS 181
Query: 1024 DITQGLPKVEQVLEVRSIDSISMNLEKRIDAWNERITGILGIPWSRKISMSRKLLQDNIS 1083
DITQGLPKVEQ+LE+RSIDSISMNLEKRIDAWNE IT ILGIPW I + Q IS
Sbjct: 182 DITQGLPKVEQILEIRSIDSISMNLEKRIDAWNECITRILGIPWGFLIGAELTIAQSRIS 361
>TC82897 similar to GP|16604396|gb|AAL24204.1 AT5g18630/T1A4_10 {Arabidopsis
thaliana}, partial (35%)
Length = 678
Score = 32.3 bits (72), Expect = 1.0
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = +2
Query: 996 ATIHGHYGEMLYEGDILVTFIYEKSRSGDITQGLPKVEQVLEV 1038
ATI Y +Y D+ F + SR GD+T+G +E V++V
Sbjct: 224 ATILVEYASAVYLSDLTQLFTWTCSRCGDLTKGFEIIELVVDV 352
>BE249090
Length = 647
Score = 30.4 bits (67), Expect = 3.9
Identities = 22/72 (30%), Positives = 38/72 (52%)
Frame = -3
Query: 539 IKVDRFFFIPQEVHILSESSSIMVRNNSIIGVNTPITLNKKSRVGGLVRVEKNKKKIELK 598
IKV+ F ++ ++ L + ++ + I+G+ LNK V G R++KN+K IE +
Sbjct: 213 IKVEVFIYL*EK---LVTTHKLIQVQH*ILGLTNIDELNK*EMVKGDNRIKKNRKVIERR 43
Query: 599 IFSGDIHFPGEI 610
I + GEI
Sbjct: 42 IIESFVT*KGEI 7
>TC80669 homologue to PIR|S53498|S53498 dnaK-type molecular chaperone
HSP71.2 - garden pea, partial (44%)
Length = 955
Score = 29.6 bits (65), Expect = 6.6
Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 3/57 (5%)
Frame = -1
Query: 785 NPFFSIYKENIQQSLSQ---KHGTIRMLLNRNKENRSFIILSSSNCFQMGPFNNVKY 838
+PFF+ K NI+ S SQ KH +RSF+I + SNC NN ++
Sbjct: 760 DPFFNSKKRNIESSTSQVKNKHVLFAF-------SRSFLIQTISNCSSSRLINNPQH 611
>TC77420 weakly similar to GP|19310745|gb|AAL85103.1 unknown protein
{Arabidopsis thaliana}, partial (17%)
Length = 1134
Score = 29.6 bits (65), Expect = 6.6
Identities = 26/87 (29%), Positives = 47/87 (53%), Gaps = 10/87 (11%)
Frame = +3
Query: 618 AILIPPEMVKKK--------ILRNQKKKTNWRYIQWITTTKKKYFVLVRPVILY--DIAD 667
AILI +MV+KK IL +Q+ KTN ++ T+K LV P +++ I+
Sbjct: 219 AILISMKMVRKKVLLTWNICILHHQENKTNLSLYKFH*TSK-----LVTPQMMFY*KISQ 383
Query: 668 SINLVKLFPQDLFKEWDNLELKVLNFI 694
INL+K+ + + ++ +L L+ + +
Sbjct: 384 KINLLKMIKKKMCHKF-SLRLRKMKLV 461
>AL389085
Length = 577
Score = 29.3 bits (64), Expect = 8.6
Identities = 21/73 (28%), Positives = 30/73 (40%)
Frame = -1
Query: 874 FSFYHLITHNKISSIKKNLQLNKFKETFQVIKYYLMDENERIYKPDLYNNIILNPFHLNW 933
F + L KI I + N FK T+ +YL N P+ Y NI+ +H
Sbjct: 541 FIKFEL*RKRKIRKISYRQKKNFFKHTYP--SWYLQSHNHVCV*PESYTNILKYMYHKQN 368
Query: 934 DFIHPNYCEKTFP 946
P C++ FP
Sbjct: 367 KIFFP--CKQ*FP 335
>BE317600
Length = 334
Score = 29.3 bits (64), Expect = 8.6
Identities = 14/57 (24%), Positives = 25/57 (43%)
Frame = +3
Query: 833 FNNVKYHNGIKEEINQFKRNHKIPIKISLGPLGVAPQIANFFSFYHLITHNKISSIK 889
FN + +H G+ I + N ++P IS + + ++ HL+ N S K
Sbjct: 45 FNALNHHEGLAHNICMYVVNFEVPNSISTDKIALNHLCKIYYYIVHLLASNIFSCFK 215
>TC77696 similar to GP|2673912|gb|AAB88646.1| expressed protein {Arabidopsis
thaliana}, partial (83%)
Length = 953
Score = 29.3 bits (64), Expect = 8.6
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = +3
Query: 88 LWSPQGGCGYCCTNIRCWISYTETC*SGSTHCCTPNRLRYRSRDFCKYS 136
L +G C +CC I CW +Y+ R + +R+FC+ S
Sbjct: 372 LQDEEGSCKWCCWWICCWCNYSGL-----------QRKEHLNRNFCRIS 485
>TC77270 similar to GP|16973465|gb|AAL32300.1 lethal leaf spot 1-like
protein {Lycopersicon esculentum}, partial (83%)
Length = 1915
Score = 29.3 bits (64), Expect = 8.6
Identities = 13/42 (30%), Positives = 16/42 (37%)
Frame = -1
Query: 79 FFNRIYYFLLWSPQGGCGYCCTNIRCWISYTETC*SGSTHCC 120
FF F W CG+CC C+ + SGS C
Sbjct: 328 FFINFVLFSFWVRFSFCGWCCNTSNCY*KVSSVSVSGSC*IC 203
>TC88358 proteinase inhibitor 20 [Medicago truncatula]
Length = 806
Score = 29.3 bits (64), Expect = 8.6
Identities = 17/53 (32%), Positives = 28/53 (52%)
Frame = -2
Query: 774 ISYNESDRININPFFSIYKENIQQSLSQKHGTIRMLLNRNKENRSFIILSSSN 826
++ N ++R NI F S S KH I ++ ++ + + SFII+ SSN
Sbjct: 700 LNRNSNNRFNILSFIS--------SSINKHNLINLMFSQIQTSSSFIIIKSSN 566
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.327 0.142 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,090,427
Number of Sequences: 36976
Number of extensions: 627194
Number of successful extensions: 4333
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 3152
Number of HSP's successfully gapped in prelim test: 155
Number of HSP's that attempted gapping in prelim test: 1044
Number of HSP's gapped (non-prelim): 3482
length of query: 1085
length of database: 9,014,727
effective HSP length: 106
effective length of query: 979
effective length of database: 5,095,271
effective search space: 4988270309
effective search space used: 4988270309
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0269b.9