BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0269b.6
(141 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC76737 homologue to SP|P06379|RK2_TOBAC Chloroplast 50S ribosom... 177 1e-45
TC87562 similar to GP|17644114|gb|AAK95391.1 ribosomal protein L... 55 7e-09
TC76323 homologue to GP|12054507|emb|CAC20221. ribosomal protein... 46 4e-06
TC76359 homologue to GP|12054507|emb|CAC20221. ribosomal protein... 39 0.001
TC85318 similar to PIR|D96568|D96568 probable histone H2A 14481... 30 0.42
TC81708 similar to GP|12083536|gb|AAG48845.1 hypothetical protei... 28 0.95
TC79815 similar to GP|2181184|emb|CAA73905.1 JR3 protein {Arabid... 28 1.6
BQ147334 similar to GP|2293383|emb| 1-evidence=predicted by cont... 28 1.6
TC81813 similar to GP|22137164|gb|AAM91427.1 AT3g55400/T22E16_60... 28 1.6
TC86465 similar to SP|Q06445|CYTI_VIGUN Cysteine proteinase inhi... 27 2.1
TC89705 similar to GP|21689785|gb|AAM67536.1 unknown protein {Ar... 27 2.1
TC88377 similar to GP|8778315|gb|AAF79324.1| F14J16.29 {Arabidop... 27 2.1
AL378429 similar to GP|9294446|dbj sulfate transporter {Arabidop... 27 2.1
BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xy... 27 2.1
AW736524 similar to GP|2181184|emb JR3 protein {Arabidopsis thal... 27 2.8
BE999686 similar to SP|O70133|DDX9 ATP-dependent RNA helicase A ... 27 2.8
CB065266 similar to GP|19033981|gb acetaldehyde dehydrogenase {P... 27 2.8
BQ137781 GP|14165182|gb zinc family member 5 protein {Homo sapie... 27 2.8
TC88376 similar to GP|8778315|gb|AAF79324.1| F14J16.29 {Arabidop... 27 3.6
TC78845 similar to GP|17473709|gb|AAL38308.1 unknown protein {Ar... 26 4.7
>TC76737 homologue to SP|P06379|RK2_TOBAC Chloroplast 50S ribosomal protein
L2. [Common tobacco] {Nicotiana tabacum}, partial (95%)
Length = 5466
Score = 177 bits (449), Expect = 1e-45
Identities = 82/91 (90%), Positives = 85/91 (93%)
Frame = -2
Query: 9 PPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTVAEQFLDIKRTSPEGN 68
PPP GWSTGFITTPLTTGRLP+Q PALPKLFWFTPTFPTCPTVAEQF DIKRTSPEGN
Sbjct: 1340 PPPCGWSTGFITTPLTTGRLPNQRLAPALPKLFWFTPTFPTCPTVAEQFFDIKRTSPEGN 1161
Query: 69 FNVADFPSFAISFATAPAALANFPPFPNVIT 99
FNVADFPSFAISFATAPAALAN PPFP+VI+
Sbjct: 1160 FNVADFPSFAISFATAPAALANCPPFPSVIS 1068
>TC87562 similar to GP|17644114|gb|AAK95391.1 ribosomal protein L2
{Gossypium arboreum}, partial (75%)
Length = 977
Score = 55.5 bits (132), Expect = 7e-09
Identities = 40/89 (44%), Positives = 46/89 (50%), Gaps = 2/89 (2%)
Frame = -2
Query: 9 PPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTVAEQFLDIKRTSPEGN 68
PPP G STGFI TP TTG S PA L T PTVA + I +P+G+
Sbjct: 643 PPP*G*STGFIATPRTTGLRLSHLL*PAFLSLRSP*VGLETVPTVALHRVSISFLTPDGS 464
Query: 69 F--NVADFPSFAISFATAPAALANFPPFP 95
+ AD SF I FA PAA +FPP P
Sbjct: 463 WIKTYADVGSFKI-FALVPAARTSFPPCP 380
>TC76323 homologue to GP|12054507|emb|CAC20221. ribosomal protein L2
{Glycine max}, complete
Length = 1031
Score = 46.2 bits (108), Expect = 4e-06
Identities = 37/94 (39%), Positives = 47/94 (49%), Gaps = 7/94 (7%)
Frame = -2
Query: 9 PPPWGWSTGFITTPLTTGR--LPSQHFDPALPKL---FWFTPTFPTCPTVAEQFLDIKRT 63
PPPWG STGFI TP T G+ L + + ALP F P TCP +A Q L
Sbjct: 688 PPPWGCSTGFIATPRTLGQQFLFTLNLW*ALPAFNIGFSVLPPPATCPIMALQSLGTIFF 509
Query: 64 SPEGN--FNVADFPSFAISFATAPAALANFPPFP 95
+P+G+ V+ I+ A +P ALA P P
Sbjct: 508 APDGSLILEVSLSGL*LITIA*SPEALAKTPRSP 407
>TC76359 homologue to GP|12054507|emb|CAC20221. ribosomal protein L2
{Glycine max}, partial (80%)
Length = 860
Score = 38.5 bits (88), Expect = 0.001
Identities = 35/94 (37%), Positives = 45/94 (47%), Gaps = 7/94 (7%)
Frame = -2
Query: 9 PPPWGWSTGFITTPLTTGR--LPSQHFDPALPKL---FWFTPTFPTCPTVAEQFLDIKRT 63
PPP G STGFI TP T G+ L + + ALP L F P TCP +A L
Sbjct: 505 PPP*GCSTGFIATPRTLGQQFLFTLNLW*ALPALSIGFSVLPPPATCPIIALHSLGTIFF 326
Query: 64 SPEGN--FNVADFPSFAISFATAPAALANFPPFP 95
+P+G+ V+ I+ A +P LA P P
Sbjct: 325 APDGSLILEVSLSGL*LITIA*SPEHLAKAPRSP 224
>TC85318 similar to PIR|D96568|D96568 probable histone H2A 14481-15293
[imported] - Arabidopsis thaliana, complete
Length = 778
Score = 29.6 bits (65), Expect = 0.42
Identities = 25/79 (31%), Positives = 37/79 (46%), Gaps = 9/79 (11%)
Frame = -2
Query: 45 PTFPTCPTVAEQFLDIKRT--SPEGN-------FNVADFPSFAISFATAPAALANFPPFP 95
PT P C V LD +R P GN +V F S ++ FA + F PFP
Sbjct: 246 PTLP-CAIV----LDFRRRWIRPTGN*RPALEERDVGFFLSLSLRFAGVLPVVKPFAPFP 82
Query: 96 NVITHFFINRTFYKRHKTK 114
++ +HF R F++R + +
Sbjct: 81 DMFSHF--KRFFFERERER 31
>TC81708 similar to GP|12083536|gb|AAG48845.1 hypothetical protein {Oryza
sativa}, partial (6%)
Length = 605
Score = 28.5 bits (62), Expect = 0.95
Identities = 18/49 (36%), Positives = 25/49 (50%)
Frame = -1
Query: 5 PFTPPPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTV 53
P +PPPP+ +S F P + LP P LP F+ +P FP P +
Sbjct: 158 PQSPPPPFCFSPLFFLPPSSPPFLP-----PPLP--FFTSP*FPLLPFI 33
>TC79815 similar to GP|2181184|emb|CAA73905.1 JR3 protein {Arabidopsis
thaliana}, partial (46%)
Length = 850
Score = 27.7 bits (60), Expect = 1.6
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 59 DIKRTSPEGNFNVADFPSFAISFATAPAALANFPPFP 95
D++ TSP G P A++F+ APA+ P P
Sbjct: 747 DLEDTSPIGKSGTICNPKIAVTFSNAPASKIXLAPPP 637
>BQ147334 similar to GP|2293383|emb| 1-evidence=predicted by
content~1-method=genefinder;084~1-method_score=42.09~1-
evidence_end~, partial (2%)
Length = 600
Score = 27.7 bits (60), Expect = 1.6
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -1
Query: 5 PFTPPPPWGWSTGFITTPLTTGRLPSQHFDPALP 38
P T PPP GW + P +TG L S ++PA P
Sbjct: 351 PLTEPPPTGWEL-VLMPPFSTGTLTS-IWNPANP 256
>TC81813 similar to GP|22137164|gb|AAM91427.1 AT3g55400/T22E16_60
{Arabidopsis thaliana}, partial (37%)
Length = 921
Score = 27.7 bits (60), Expect = 1.6
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -2
Query: 3 GPPFTPPPPWGWS 15
GPP +PPPPW +S
Sbjct: 851 GPPSSPPPPWYFS 813
>TC86465 similar to SP|Q06445|CYTI_VIGUN Cysteine proteinase inhibitor
(Cystatin). [Cowpea] {Vigna unguiculata}, complete
Length = 660
Score = 27.3 bits (59), Expect = 2.1
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -2
Query: 35 PALPKLFWFTPTFPTCPTVA 54
P++ +LFWF T T PTVA
Sbjct: 119 PSIARLFWFPETSRTPPTVA 60
>TC89705 similar to GP|21689785|gb|AAM67536.1 unknown protein {Arabidopsis
thaliana}, partial (46%)
Length = 812
Score = 27.3 bits (59), Expect = 2.1
Identities = 20/69 (28%), Positives = 31/69 (43%), Gaps = 6/69 (8%)
Frame = +3
Query: 53 VAEQFLDIKRTSPEGNFNVADFPSFAISFATAPAALAN------FPPFPNVITHFFINRT 106
+ ++ ++IKRT P+G DF + I PA ++ F NV+ H I
Sbjct: 303 INDKQVEIKRTIPKGASQTNDFKTKKIFVGGIPATVSEDELKIFFSKHGNVVEHEII--- 473
Query: 107 FYKRHKTKR 115
+ H TKR
Sbjct: 474 --RDHTTKR 494
>TC88377 similar to GP|8778315|gb|AAF79324.1| F14J16.29 {Arabidopsis
thaliana}, partial (23%)
Length = 644
Score = 27.3 bits (59), Expect = 2.1
Identities = 24/86 (27%), Positives = 33/86 (37%), Gaps = 9/86 (10%)
Frame = +1
Query: 20 TTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTVAEQFLDIKRTSPEGNF----NVADFP 75
T P LP P LP + P P+ PT+ + P+GN + P
Sbjct: 148 TLPSIPTTLPPLPSIPTLPSVPTTLPPLPSMPTLPQ---------PQGNVPPLPTIPSVP 300
Query: 76 SFAI----SFATAPAALA-NFPPFPN 96
+ S T P + NFPPFP+
Sbjct: 301 KLTMPPLPSIPTNPTLPSLNFPPFPS 378
>AL378429 similar to GP|9294446|dbj sulfate transporter {Arabidopsis
thaliana}, partial (22%)
Length = 473
Score = 27.3 bits (59), Expect = 2.1
Identities = 16/52 (30%), Positives = 20/52 (37%), Gaps = 5/52 (9%)
Frame = +3
Query: 12 WGWST-----GFITTPLTTGRLPSQHFDPALPKLFWFTPTFPTCPTVAEQFL 58
W W T GF+ LTT +H PKLFW + P + L
Sbjct: 201 WSWQTIVMGFGFLAFLLTT-----RHISLRKPKLFWASAAAPLTSVILSTIL 341
>BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xylostella
granulovirus}, partial (42%)
Length = 909
Score = 27.3 bits (59), Expect = 2.1
Identities = 11/28 (39%), Positives = 13/28 (46%)
Frame = -3
Query: 2 GGPPFTPPPPWGWSTGFITTPLTTGRLP 29
GG P PPP GW G + G +P
Sbjct: 412 GGAPPLPPPSPGWWAGLVWASSAVGGVP 329
>AW736524 similar to GP|2181184|emb JR3 protein {Arabidopsis thaliana},
partial (22%)
Length = 637
Score = 26.9 bits (58), Expect = 2.8
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -3
Query: 59 DIKRTSPEGNFNVADFPSFAISFATAPAALANFPPFP 95
D++ TSP G P A++F+ APA+ P P
Sbjct: 611 DLEDTSPIGKSGTICNPKIAVTFSNAPASKIFLAPPP 501
>BE999686 similar to SP|O70133|DDX9 ATP-dependent RNA helicase A (Nuclear DNA
helicase II) (NDH II) (DEAD-box protein 9) (mHEL-5).,
partial (1%)
Length = 424
Score = 26.9 bits (58), Expect = 2.8
Identities = 17/42 (40%), Positives = 18/42 (42%), Gaps = 1/42 (2%)
Frame = +3
Query: 2 GGPPF-TPPPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFW 42
GGPP TPPPP W T P G P + K FW
Sbjct: 219 GGPPGGTPPPPKKWGG*KKTPPKNPGCPPPKTPPGGGKKNFW 344
>CB065266 similar to GP|19033981|gb acetaldehyde dehydrogenase {Pseudomonas
putida}, partial (48%)
Length = 464
Score = 26.9 bits (58), Expect = 2.8
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +1
Query: 4 PPFTPPPPWGWSTG 17
P TPP PWGW G
Sbjct: 10 PRVTPPRPWGWWPG 51
>BQ137781 GP|14165182|gb zinc family member 5 protein {Homo sapiens}, partial
(3%)
Length = 1213
Score = 26.9 bits (58), Expect = 2.8
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +1
Query: 4 PPFTPPPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFWF 43
PP TPP W+ G + P + ++ S AL + +F
Sbjct: 295 PPPTPPRAGSWAAGILIHPSSLRKIISNQIIVALVSVVYF 414
>TC88376 similar to GP|8778315|gb|AAF79324.1| F14J16.29 {Arabidopsis
thaliana}, partial (25%)
Length = 706
Score = 26.6 bits (57), Expect = 3.6
Identities = 22/74 (29%), Positives = 29/74 (38%), Gaps = 11/74 (14%)
Frame = +1
Query: 34 DPALPKLFWFTPTFPTCPTVAEQFLDIKRTSPEGNFNVADFPSFAI----------SFAT 83
+P LP + P P+ PT L T P+ NV P+ S T
Sbjct: 178 NPTLPSIPTTLPPLPSIPTTLPP-LPSMPTLPQPQGNVPPLPTIPSMPKLTMPPLPSIPT 354
Query: 84 APAALA-NFPPFPN 96
P + NFPPFP+
Sbjct: 355 NPTLPSLNFPPFPS 396
>TC78845 similar to GP|17473709|gb|AAL38308.1 unknown protein {Arabidopsis
thaliana}, partial (26%)
Length = 702
Score = 26.2 bits (56), Expect = 4.7
Identities = 17/45 (37%), Positives = 22/45 (48%), Gaps = 2/45 (4%)
Frame = +2
Query: 5 PFTPPPPWGWSTGFITTPLTTGRLPSQHFDPALPKLFW--FTPTF 47
P PPPP+ + T T+PL + H P + LF FTP F
Sbjct: 206 PILPPPPFHFRTPSTTSPLFKITI---HTPP*IHPLFLPPFTPNF 331
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.329 0.144 0.500
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,863,000
Number of Sequences: 36976
Number of extensions: 95975
Number of successful extensions: 1010
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 952
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1000
length of query: 141
length of database: 9,014,727
effective HSP length: 86
effective length of query: 55
effective length of database: 5,834,791
effective search space: 320913505
effective search space used: 320913505
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0269b.6