BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0269b.10
(382 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG644750 homologue to PIR|A05205|A0 hypothetical protein 1708 - ... 135 3e-32
TC87825 similar to GP|21553995|gb|AAM63076.1 PP2A regulatory sub... 32 0.41
TC92481 similar to GP|15450341|gb|AAK96464.1 AT4g11980/F16J13_50... 31 0.70
TC78211 weakly similar to GP|15624010|dbj|BAB68064. sulfate tran... 31 0.91
TC89238 similar to SP|Q01877|HS71_PUCGR Heat shock protein HSS1.... 30 1.2
TC77674 similar to GP|16226747|gb|AAL16250.1 AT5g05080/MUG13_6 {... 30 1.6
TC85951 similar to GP|18000068|gb|AAL54885.1 cytochrome P450-dep... 30 2.0
BQ750925 weakly similar to GP|3859711|emb| CBS domain protein {C... 29 2.6
TC78649 weakly similar to GP|6651029|gb|AAF22136.1| gamma-glutam... 28 4.5
TC83143 28 4.5
BG454188 similar to GP|22316556|emb monomeric G-protein {Arabido... 28 4.5
TC77388 similar to PIR|E96553|E96553 probable RNA-binding protei... 28 5.9
TC92842 weakly similar to GP|17861816|gb|AAL39385.1 GM01519p {Dr... 28 5.9
BQ141405 similar to GP|17065622|gb| unknown protein {Arabidopsis... 28 7.7
TC84037 28 7.7
TC93049 similar to GP|11993488|gb|AAG42762.1 ubiquitin-specific ... 28 7.7
>BG644750 homologue to PIR|A05205|A0 hypothetical protein 1708 - common
tobacco chloroplast, partial (14%)
Length = 756
Score = 135 bits (339), Expect = 3e-32
Identities = 62/70 (88%), Positives = 64/70 (90%)
Frame = +3
Query: 1 KPFLLDDHDTSQKSRFLINGGTISPFLFNKIPKWMIDSFDTIKNRRKFFDNTDSYFSMIS 60
KPFLLDDHDTSQKS+FLINGGTISPFLFNKIPKWMIDSF T NRRK FDN DSYFSMI
Sbjct: 540 KPFLLDDHDTSQKSKFLINGGTISPFLFNKIPKWMIDSFHTRNNRRKSFDNPDSYFSMIF 719
Query: 61 HDEDNWLNPV 70
HD+DNWLNPV
Sbjct: 720 HDQDNWLNPV 749
>TC87825 similar to GP|21553995|gb|AAM63076.1 PP2A regulatory subunit
{Arabidopsis thaliana}, partial (94%)
Length = 1448
Score = 32.0 bits (71), Expect = 0.41
Identities = 28/120 (23%), Positives = 44/120 (36%)
Frame = +2
Query: 214 NFERTYCQPLSDMNLPDSEGKNLHQYLKFNSNMGLIHIPCSEKYLPSENRKKGIPCLKKC 273
N R + P+ +M + D L + + IH +E E KKG L KC
Sbjct: 29 NLRR*FSFPMGEMKMEDMPLPALFEQAR------KIHATATESGADQELVKKGCEALNKC 190
Query: 274 LEKGQMYRTFQRDSVFSTLSKWNLFQTYIPWFLTSTGYKYLNFIFLDTFSDLLPILSSSQ 333
F + +S +L +P++L K + D +PIL +SQ
Sbjct: 191 ENMVNRLGLFSANETKEDISTTDLKYILVPYYLAELTEKIAH-------DDRIPILKASQ 349
>TC92481 similar to GP|15450341|gb|AAK96464.1 AT4g11980/F16J13_50
{Arabidopsis thaliana}, partial (18%)
Length = 762
Score = 31.2 bits (69), Expect = 0.70
Identities = 19/69 (27%), Positives = 32/69 (45%), Gaps = 8/69 (11%)
Frame = +3
Query: 89 RFLNNRYHFCFYCNKRLP-------FYVEKACINNYDFTYGQFLNILFIRNK-RFSLCGG 140
R+LNN Y +C+Y NK P + K ++ L + NK ++C
Sbjct: 453 RYLNNDYGYCYYLNKSFPKERPSGMCWGTKVVLSRRFRVQSLLLKFSPLPNKLTTNICQS 632
Query: 141 KKKHAFLER 149
KKK+++L +
Sbjct: 633 KKKNSYLNK 659
>TC78211 weakly similar to GP|15624010|dbj|BAB68064. sulfate
transporter-like protein {Oryza sativa (japonica
cultivar-group)}, partial (66%)
Length = 2090
Score = 30.8 bits (68), Expect = 0.91
Identities = 17/51 (33%), Positives = 28/51 (54%)
Frame = +3
Query: 49 FDNTDSYFSMISHDEDNWLNPVKPFHRSSLISSFYKANRLRFLNNRYHFCF 99
+DN+ S + H +N +P+ R++++SS L F NN YH+CF
Sbjct: 429 WDNSSSIIAYC*HS----INCSRPYSRTNIVSS------LDFHNNLYHWCF 551
>TC89238 similar to SP|Q01877|HS71_PUCGR Heat shock protein HSS1. {Puccinia
graminis}, partial (32%)
Length = 1015
Score = 30.4 bits (67), Expect = 1.2
Identities = 20/56 (35%), Positives = 26/56 (45%), Gaps = 3/56 (5%)
Frame = +3
Query: 91 LNNRYHFCFYCNKR---LPFYVEKACINNYDFTYGQFLNILFIRNKRFSLCGGKKK 143
+N H FYCN L Y+ IN Y FT+ N+ + K FS+ KKK
Sbjct: 813 INLLQH*FFYCNLF*LFLLLYILSHNINMYKFTHN*KFNLEIFKYKNFSIAFFKKK 980
>TC77674 similar to GP|16226747|gb|AAL16250.1 AT5g05080/MUG13_6 {Arabidopsis
thaliana}, partial (82%)
Length = 1199
Score = 30.0 bits (66), Expect = 1.6
Identities = 17/41 (41%), Positives = 23/41 (55%), Gaps = 2/41 (4%)
Frame = +2
Query: 145 AFLERDTISPIESRVFN--ILILNDFPQSGDEGYNLYKSFH 183
A +E +P ES VF + + +DFP S +GY L K FH
Sbjct: 326 ADIEGPAATPYESGVFRMKLKLSHDFPHSPPKGYFLTKIFH 448
>TC85951 similar to GP|18000068|gb|AAL54885.1 cytochrome P450-dependent
fatty acid hydroxylase {Vicia sativa}, partial (45%)
Length = 742
Score = 29.6 bits (65), Expect = 2.0
Identities = 27/88 (30%), Positives = 40/88 (44%), Gaps = 6/88 (6%)
Frame = +1
Query: 264 KKGIPCLKKCLEKGQMYRTFQRDSVFSTLSKWNLFQTYIP--WFLTSTGYKYLNFIFLDT 321
K PC +K L + F D +FS + FQ I F T + K++ +
Sbjct: 217 KTNFPCYRKGLTLKRSVGDFLGDGIFSADGETWKFQRQISSHEFNTKSLRKFVETVVEVE 396
Query: 322 FSD-LLPILSSSQKFVSI---FHDIMHR 345
+D LLPILS + K ++ F DI+ R
Sbjct: 397 LNDRLLPILSEASKNQTVLLDFQDILQR 480
>BQ750925 weakly similar to GP|3859711|emb| CBS domain protein {Candida
albicans}, partial (3%)
Length = 659
Score = 29.3 bits (64), Expect = 2.6
Identities = 17/64 (26%), Positives = 29/64 (44%), Gaps = 1/64 (1%)
Frame = +2
Query: 66 WLNPVKPFHRSSLISSFYKANRLRFLNNRYHFCFYCN-KRLPFYVEKACINNYDFTYGQF 124
W+N + F ++ S + N+ L + HFC C P + AC+ FT+ Q
Sbjct: 26 WINATESFLPMAIKSIYRSLNQTWSLTSTAHFCAICKVMPAPSSIHMACMQVMLFTHPQP 205
Query: 125 LNIL 128
+ I+
Sbjct: 206 MLII 217
>TC78649 weakly similar to GP|6651029|gb|AAF22136.1| gamma-glutamylcysteine
synthetase precursor {Phaseolus vulgaris}, partial (32%)
Length = 1052
Score = 28.5 bits (62), Expect = 4.5
Identities = 15/42 (35%), Positives = 21/42 (49%)
Frame = -1
Query: 217 RTYCQPLSDMNLPDSEGKNLHQYLKFNSNMGLIHIPCSEKYL 258
RT C S + P KN H L+ NS+ ++PCS + L
Sbjct: 551 RTLCSETSSYSRPT*HRKNYHNKLRLNSH----NMPCSTRQL 438
>TC83143
Length = 1277
Score = 28.5 bits (62), Expect = 4.5
Identities = 32/152 (21%), Positives = 60/152 (39%), Gaps = 14/152 (9%)
Frame = -2
Query: 209 EGQIVNFERTYCQPLS---DMNLPDSEGKNLHQYLKFNSNMGLIHIPCSEK-------YL 258
+G+ ++FE T + ++++P G L Q ++ S +G +P + +L
Sbjct: 982 DGEFLSFEETVVGVAALKFEVDIPRKSGCYLLQPFEYRSVVGNSVLPFGPEIQTVGYVFL 803
Query: 259 PSENRKKGI----PCLKKCLEKGQMYRTFQRDSVFSTLSKWNLFQTYIPWFLTSTGYKYL 314
P E GI P K+ + G ++ F+ SV + ++P G
Sbjct: 802 PLEKVMFGIVDVLPFEKETQKAGCVFHQFEYQSVEGNFEIQTVGYVFLPLEKVMFG---- 635
Query: 315 NFIFLDTFSDLLPILSSSQKFVSIFHDIMHRS 346
D+LP +QK +FH ++S
Sbjct: 634 -------IVDMLPFEKETQKAGCVFHQFEYQS 560
>BG454188 similar to GP|22316556|emb monomeric G-protein {Arabidopsis
thaliana}, partial (26%)
Length = 669
Score = 28.5 bits (62), Expect = 4.5
Identities = 10/30 (33%), Positives = 18/30 (59%)
Frame = +2
Query: 92 NNRYHFCFYCNKRLPFYVEKACINNYDFTY 121
+N +HFCF + +PF E+ ++ F+Y
Sbjct: 26 SNPFHFCFILFRFIPFRSERHTYTHFHFSY 115
>TC77388 similar to PIR|E96553|E96553 probable RNA-binding protein
35994-37391 [imported] - Arabidopsis thaliana, partial
(64%)
Length = 1029
Score = 28.1 bits (61), Expect = 5.9
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -3
Query: 74 HRSSLISSFYKANRLRFLNNRYHFC 98
H + +F K ++RFLNNRY FC
Sbjct: 832 HNKLELITFNK*CKIRFLNNRYVFC 758
>TC92842 weakly similar to GP|17861816|gb|AAL39385.1 GM01519p {Drosophila
melanogaster}, partial (7%)
Length = 669
Score = 28.1 bits (61), Expect = 5.9
Identities = 23/96 (23%), Positives = 44/96 (44%), Gaps = 2/96 (2%)
Frame = +2
Query: 157 SRVFNILILNDFPQSGDEGYNLYKSFHFPIRSDPFVHRAIYSIAD--ISVTPLTEGQIVN 214
++ + +L +D P+SGD GY + F V++A+ + AD +++ L+ +
Sbjct: 287 AQYWQLLASSDDPRSGDYGYTQDQMHKFGAHQGAAVYQALDAAADRNVNIRLLSHSGVY- 463
Query: 215 FERTYCQPLSDMNLPDSEGKNLHQYLKFNSNMGLIH 250
T+ S + S KN+ LK G++H
Sbjct: 464 --PTFTLEPSKLAYGRSNVKNVTLLLKDWRGSGIVH 565
>BQ141405 similar to GP|17065622|gb| unknown protein {Arabidopsis thaliana},
partial (12%)
Length = 577
Score = 27.7 bits (60), Expect = 7.7
Identities = 16/51 (31%), Positives = 24/51 (46%)
Frame = -1
Query: 228 LPDSEGKNLHQYLKFNSNMGLIHIPCSEKYLPSENRKKGIPCLKKCLEKGQ 278
L D+ G + +S I +P S +LP ++ PCL CLEK +
Sbjct: 373 LYDTNGPFFEDNISSSSKFSKISLPSSFLFLPLDD-----PCLLFCLEKSE 236
>TC84037
Length = 505
Score = 27.7 bits (60), Expect = 7.7
Identities = 14/61 (22%), Positives = 26/61 (41%)
Frame = +2
Query: 35 MIDSFDTIKNRRKFFDNTDSYFSMISHDEDNWLNPVKPFHRSSLISSFYKANRLRFLNNR 94
+I + K+R + FS + DNW +P+ P +S + FY + F +
Sbjct: 41 IIKTLKKSKHRGDWTAEHSGDFSPFNSKRDNWRSPITPCTNNSAKNHFYNSGSHNFSSKS 220
Query: 95 Y 95
+
Sbjct: 221 F 223
>TC93049 similar to GP|11993488|gb|AAG42762.1 ubiquitin-specific protease 24
{Arabidopsis thaliana}, partial (27%)
Length = 823
Score = 27.7 bits (60), Expect = 7.7
Identities = 21/66 (31%), Positives = 26/66 (38%), Gaps = 9/66 (13%)
Frame = -2
Query: 239 YLKFNSNMGLI--HIPCSE-------KYLPSENRKKGIPCLKKCLEKGQMYRTFQRDSVF 289
YL N+GLI H+ C K P R GI C+ M +F
Sbjct: 459 YLFVEKNIGLIMQHLICCNSKDRCVIKSQPPTIRVLGISCVMPF*RLPSMMSNCSNKFIF 280
Query: 290 STLSKW 295
STLS+W
Sbjct: 279 STLSRW 262
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.141 0.447
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,698,297
Number of Sequences: 36976
Number of extensions: 276935
Number of successful extensions: 2004
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1984
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2003
length of query: 382
length of database: 9,014,727
effective HSP length: 98
effective length of query: 284
effective length of database: 5,391,079
effective search space: 1531066436
effective search space used: 1531066436
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0269b.10