Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0265.8
         (765 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BE997611                                                               39  0.007
TC86635 weakly similar to GP|9758600|dbj|BAB09233.1 gene_id:MDF2...    32  1.2
TC82133 similar to PIR|G84597|G84597 probable XAP-5 protein [Hom...    32  1.2
AW691010 similar to GP|8656003|gb| Contains similarity to formin...    31  2.0
TC86908 similar to PIR|G96708|G96708 hypothetical protein T26J14...    30  2.6
TC77435 similar to GP|7211427|gb|AAF40306.1| RNA helicase {Vigna...    30  4.5
TC78700 similar to GP|21554835|gb|AAM63703.1 unknown {Arabidopsi...    29  5.9
TC87549 similar to PIR|T01734|T01734 hypothetical protein A_IG00...    29  5.9
TC85955 similar to GP|3860319|emb|CAA10127.1 nucleolar protein {...    29  7.7
TC93570 similar to GP|21553436|gb|AAM62529.1 unknown {Arabidopsi...    29  7.7
BG587226 similar to GP|10176955|d phosphatidylinositol 4-kinase ...    29  7.7

>BE997611 
          Length = 547

 Score = 38.9 bits (89), Expect = 0.007
 Identities = 33/130 (25%), Positives = 57/130 (43%)
 Frame = -2

Query: 436 NVNDEFVGDIEPLGENKKKQKEKEEEKRKVELEKKFTKVPFPPFPTNIAKRRLEKQFSKF 495
           N+ DE +  +E + E+K + ++  EEK              PP            +F+  
Sbjct: 516 NMEDEHINAVE-VAEDKPQTEDA*EEKE------------VPP------------KFNAL 412

Query: 496 ISMFKKLRVELPFSEVLEKMPQYAKFIKEILSKKRRLSEENEIVELTEECSVILQRKLPP 555
             + K  +V +P +E +E++P   KF++E+L     LSEE E + L+ E     +     
Sbjct: 411 HHILKGYKVNMPITETVEQIPCCIKFLQELLKTNANLSEE-EFISLSSEFHHTYEVPAVV 235

Query: 556 KRKDPGSFTL 565
           +    G FTL
Sbjct: 234 RFDGEGCFTL 205


>TC86635 weakly similar to GP|9758600|dbj|BAB09233.1
           gene_id:MDF20.10~pir||T08929~similar to unknown protein
           {Arabidopsis thaliana}, partial (32%)
          Length = 1963

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 20/76 (26%), Positives = 35/76 (45%)
 Frame = +2

Query: 426 EPKQKIVENNNVNDEFVGDIEPLGENKKKQKEKEEEKRKVELEKKFTKVPFPPFPTNIAK 485
           E  +  ++ +   D+  G     G  K+ + +  EEK KV+  KK  K+P P  PT+   
Sbjct: 149 EADEDTIDKSKEEDKAEGSKGEKGSKKRARGKVNEEKVKVK--KKELKLPEPKTPTSDRP 322

Query: 486 RRLEKQFSKFISMFKK 501
            R  K   + +++  K
Sbjct: 323 VRERKSVERLVALIDK 370


>TC82133 similar to PIR|G84597|G84597 probable XAP-5 protein [Homo sapiens]
           [imported] - Arabidopsis thaliana, partial (60%)
          Length = 926

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 16/48 (33%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
 Frame = +3

Query: 424 LSEPKQKIVENNNVNDEFVGDIEPLGENKKKQKEKEEEKRKV-ELEKK 470
           LSE K++  E + + D +VG  +     ++ +K++E E+RK+ EL+ K
Sbjct: 135 LSEEKKRAREMSGMGDGYVGTAQDAVRIRRLEKQREAERRKIQELKSK 278


>AW691010 similar to GP|8656003|gb| Contains similarity to formin binding
           protein 11 from Mus musculus gb|AF135439 and contains,
           partial (18%)
          Length = 655

 Score = 30.8 bits (68), Expect = 2.0
 Identities = 29/123 (23%), Positives = 54/123 (43%), Gaps = 11/123 (8%)
 Frame = +3

Query: 437 VNDEFVGDIEPLGENKKKQKEKEEEKRKVELEKKFTK-----VPFPPFPTNIAKRRLEKQ 491
           +ND+  G ++ LGE K+   E   +++K E E+K  K       F        +     +
Sbjct: 75  INDKRYGALKSLGERKQAFNEYLSQRKKQEAEEKRMKHKKAREDFRKMLEESTELTSSIR 254

Query: 492 FSKFISMFKKLRVELPFSEVLEKMPQYAKFIKEILSKKR-RLSEEN-----EIVELTEEC 545
           +SK I++F+            ++      F++E+L+K+R ++ EE      E  +  E C
Sbjct: 255 YSKAIAIFENDDRFKAVERERDRKDMIESFLEELLNKERAKVLEERKRNTVEYRKFLESC 434

Query: 546 SVI 548
             I
Sbjct: 435 DFI 443


>TC86908 similar to PIR|G96708|G96708 hypothetical protein T26J14.4
           [imported] - Arabidopsis thaliana, partial (65%)
          Length = 1587

 Score = 30.4 bits (67), Expect = 2.6
 Identities = 16/56 (28%), Positives = 31/56 (54%), Gaps = 3/56 (5%)
 Frame = +1

Query: 450 ENKKKQKEKEEEKRKVELEKKFTKVPFPP---FPTNIAKRRLEKQFSKFISMFKKL 502
           + KKK+K+K+++K+   L    + +  PP   F  N+ ++ +    SKF +M  K+
Sbjct: 88  KKKKKKKKKKKKKKTSRLVPFISYISLPPSLFFLLNVFQKVITNSTSKFSTMHNKI 255


>TC77435 similar to GP|7211427|gb|AAF40306.1| RNA helicase {Vigna radiata},
           partial (67%)
          Length = 1828

 Score = 29.6 bits (65), Expect = 4.5
 Identities = 23/91 (25%), Positives = 41/91 (44%), Gaps = 12/91 (13%)
 Frame = +1

Query: 395 RKTPGMFPSDTVINPKENCSAITLRSGATLSEPKQKIVENNNVNDEFVGDIEPLGENKKK 454
           R  P  F S T+ +       +++ S  + S  K+K+++N+ V++      +    +KK+
Sbjct: 82  RFIPSRFHSSTMPSLPITNDTVSMESPKSKSSKKKKLLQNDVVSEVEAVSAKKKESSKKR 261

Query: 455 QKEK------------EEEKRKVELEKKFTK 473
           +K              EE  RKV+ EKK  K
Sbjct: 262 KKSSDDDEETKSDISSEEGSRKVKKEKKKKK 354


>TC78700 similar to GP|21554835|gb|AAM63703.1 unknown {Arabidopsis
           thaliana}, partial (5%)
          Length = 687

 Score = 29.3 bits (64), Expect = 5.9
 Identities = 21/53 (39%), Positives = 29/53 (54%)
 Frame = +2

Query: 419 RSGATLSEPKQKIVENNNVNDEFVGDIEPLGENKKKQKEKEEEKRKVELEKKF 471
           +SG+   E K+K V   N++ E VGD       KKK+K K +E  K   EKK+
Sbjct: 428 KSGSGEEEEKEKEV---NLDGEGVGD-------KKKKKRKRDEVEKEWEEKKY 556


>TC87549 similar to PIR|T01734|T01734 hypothetical protein A_IG002N01.30 -
           Arabidopsis thaliana (fragment), partial (38%)
          Length = 1766

 Score = 29.3 bits (64), Expect = 5.9
 Identities = 50/216 (23%), Positives = 87/216 (40%), Gaps = 9/216 (4%)
 Frame = +2

Query: 485 KRRLEKQFSKFISMFKKLRVELPFSEVLEKMPQYAKFIKEILSKKRRLSEENEIVELTEE 544
           KRR E  F   I   KKL+  L   ++L   P     ++E+   +R L      ++   +
Sbjct: 458 KRRKELPFDNVIQKDKKLKFVLKVRKILVSKPTRVMSLQELGKYRRELG-----LDKKRK 622

Query: 545 CSVILQRKLPPKRKDPGSFTLPVNFGASKQVRALCDLGSSVNLMPLSM---FERLNVGEL 601
             VIL       R+ PG F + V  G       +      + L  L +    E + V +L
Sbjct: 623 LIVIL-------RRFPGVFEI-VEDGCYSLKFKMTSEAEKLYLEELRVRNEMEDVVVTKL 778

Query: 602 KPTMMMLQLADRSIVTPWGVVEDVLVRVGEF------EFPVDFVIIDMDEDSKIPLILGR 655
           +  ++M+ L  R ++   G + + L    EF       +P  F ++  +    + L    
Sbjct: 779 R-KLLMMPLEKRILLEKIGHLANDLGLPREFRDTICHRYPEFFKVVQTERGPALELTHWD 955

Query: 656 PFLATSQAKINVGKGTISLRVADEKIGFNIFDLKPK 691
           P LA S A+++  +  I   V ++ +   I D  PK
Sbjct: 956 PHLAVSAAELSAEENRIR-EVEEQNL---IIDRAPK 1051


>TC85955 similar to GP|3860319|emb|CAA10127.1 nucleolar protein {Cicer
           arietinum}, partial (98%)
          Length = 1981

 Score = 28.9 bits (63), Expect = 7.7
 Identities = 17/44 (38%), Positives = 24/44 (53%), Gaps = 1/44 (2%)
 Frame = +2

Query: 655 RPFLATSQAKINVGK-GTISLRVADEKIGFNIFDLKPKPVEKND 697
           R  L T+  K+  GK    SL VA+ KIG +I +    PV+ N+
Sbjct: 311 RTVLETNLPKVKEGKKAKFSLGVAESKIGSHIHEATKIPVQSNE 442


>TC93570 similar to GP|21553436|gb|AAM62529.1 unknown {Arabidopsis
           thaliana}, partial (57%)
          Length = 759

 Score = 28.9 bits (63), Expect = 7.7
 Identities = 35/137 (25%), Positives = 57/137 (41%), Gaps = 8/137 (5%)
 Frame = +1

Query: 450 ENKKKQKEKEEEKRKVELEKKFTKVPFPPFPTNIAKRRLEKQFSKFISMFKKLRVELPFS 509
           + KKK+   +++         FT      FPT+I+ +      + F    K L ++ P S
Sbjct: 1   KKKKKKSSNQQKNMSAITTLTFTNTTNITFPTSISTKNPIFHTTHFT---KPLHLQNPLS 171

Query: 510 EVLEKMPQYAKFIKEILSKKRRLSEENEIVELTEECSVILQRKLPPKRKDPGSFTLP--- 566
             L K       I  I+  K  +  E+E+  + E+  +   +KLP K K   + +LP   
Sbjct: 172 ISLRKP------ISTIVLCKGSVESESEVPFVPEDEWL---QKLPEKTKPLYTHSLPCIE 324

Query: 567 -----VNFGASKQVRAL 578
                + F  SK  RAL
Sbjct: 325 AWLKSLGFNQSKDDRAL 375


>BG587226 similar to GP|10176955|d phosphatidylinositol 4-kinase {Arabidopsis
           thaliana}, partial (10%)
          Length = 473

 Score = 28.9 bits (63), Expect = 7.7
 Identities = 18/47 (38%), Positives = 22/47 (46%)
 Frame = +3

Query: 439 DEFVGDIEPLGENKKKQKEKEEEKRKVELEKKFTKVPFPPFPTNIAK 485
           D  + D E LG   K+QKEK       + EK  TK P P  P+   K
Sbjct: 201 DRSIEDSELLGS--KRQKEKHPGSPMPQSEKSSTKPPLPINPSQFRK 335


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.337    0.147    0.466 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,541,054
Number of Sequences: 36976
Number of extensions: 304092
Number of successful extensions: 2172
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1220
Number of HSP's successfully gapped in prelim test: 100
Number of HSP's that attempted gapping in prelim test: 917
Number of HSP's gapped (non-prelim): 1374
length of query: 765
length of database: 9,014,727
effective HSP length: 104
effective length of query: 661
effective length of database: 5,169,223
effective search space: 3416856403
effective search space used: 3416856403
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 62 (28.5 bits)


Lotus: description of TM0265.8