BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0265.4
(822 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG587150 similar to GP|13592175|gb ppg3 {Leishmania major}, part... 77 4e-14
BG644741 71 1e-12
BE999414 39 0.008
BE997611 35 0.15
BG647389 30 2.9
TC88051 similar to GP|15982866|gb|AAL09780.1 At1g27210/T7N9_27 {... 30 2.9
TC88456 similar to GP|15487292|dbj|BAB64531. vacuolar sorting re... 30 2.9
TC76698 similar to PIR|S50766|S50766 dehydrin-related protein - ... 30 2.9
AW981373 similar to GP|8096650|dbj| ESTs C19814(E10971) AU090481... 30 4.9
TC77822 weakly similar to GP|21536834|gb|AAM61166.1 unknown {Ara... 29 6.4
TC81220 29 6.4
TC89479 similar to GP|22136170|gb|AAM91163.1 unknown protein {Ar... 29 8.4
>BG587150 similar to GP|13592175|gb ppg3 {Leishmania major}, partial (1%)
Length = 754
Score = 76.6 bits (187), Expect = 4e-14
Identities = 54/174 (31%), Positives = 88/174 (50%), Gaps = 2/174 (1%)
Frame = +2
Query: 583 KFLEVFKKLHINIPFSEALEQMLIYAKFMRDILSKKRKLSEVDETV--MLTEECSAILQR 640
K +E F+K IP + E+ A F + R+ E +E + M E + +R
Sbjct: 152 KEMESFQKRVFMIPLEKPFEE----AYFTHRLWMFFRETRETEEDIRRMFCEAREKMKKR 319
Query: 641 KIPRKRRDPGSFTIPVEIEGLSKVDALCDLGASINLMPLSMFRRLNLGEVTPTMLSLQMA 700
+K+ D G F I ++G+ ALC+ GAS+ ++P M L L +V P+ S
Sbjct: 320 ITLKKKSDSGKFAISWTMKGIEFPHALCNTGASVIILPRDMADHLGL-KVDPSKESFTFV 496
Query: 701 DRSLKTPYGIIEDVMVKVDKYVFPIDFVVLDMEEDAKIPLILGRPFLATARAKI 754
D S ++ GII D+ V++ + P+DF VLD++ + L+L R FL+T R +
Sbjct: 497 DCSQRSSGGIIRDLEVQIGNALVPVDFHVLDIKINWNSSLLLRRAFLSTGRGSV 658
>BG644741
Length = 735
Score = 71.2 bits (173), Expect = 1e-12
Identities = 36/114 (31%), Positives = 53/114 (45%)
Frame = -2
Query: 225 LSLFPFSLKDAAEEWLNSQPQGSITTWEDLAEKFTTRFFPRALLRKLKKDIMNFVQSAEE 284
L +FP SL A+ W P SI TW L + F R++P + + NFV E
Sbjct: 587 LRVFPLSLMGEADIWFTELPYNSIFTWNQLRDVFLARYYPVSKKLNHNDRVNNFVALPGE 408
Query: 285 NLYEAWERFKKLLRKCPQHNLTQAELVATFYDGLEYSWRFGLDTASSGEFDALP 338
++ +W+RF LR P H + L FY G + + + LDT + G + P
Sbjct: 407 SVSSSWDRFTSFLRSVPNHRIDDDSLKEYFYRGQDDNNKVVLDTIAGGSYGVCP 246
>BE999414
Length = 613
Score = 38.9 bits (89), Expect = 0.008
Identities = 24/65 (36%), Positives = 32/65 (48%)
Frame = +3
Query: 198 DPHAHMERFIRNCNTYRVSNVSSEAIRLSLFPFSLKDAAEEWLNSQPQGSITTWEDLAEK 257
DP HME I TYR A++ LF +L+ A W + + SI +W DL +
Sbjct: 33 DPDEHMEN-IEVVLTYRSVR---GAVKCKLFVTTLRRGAMTWFKNLRRNSIGSWGDLCHE 200
Query: 258 FTTRF 262
FTT F
Sbjct: 201 FTTHF 215
>BE997611
Length = 547
Score = 34.7 bits (78), Expect = 0.15
Identities = 17/59 (28%), Positives = 33/59 (55%)
Frame = -2
Query: 575 KNLDKQFSKFLEVFKKLHINIPFSEALEQMLIYAKFMRDILSKKRKLSEVDETVMLTEE 633
K + +F+ + K +N+P +E +EQ+ KF++++L LSE +E + L+ E
Sbjct: 441 KEVPPKFNALHHILKGYKVNMPITETVEQIPCCIKFLQELLKTNANLSE-EEFISLSSE 268
>BG647389
Length = 718
Score = 30.4 bits (67), Expect = 2.9
Identities = 15/57 (26%), Positives = 29/57 (50%)
Frame = +1
Query: 232 LKDAAEEWLNSQPQGSITTWEDLAEKFTTRFFPRALLRKLKKDIMNFVQSAEENLYE 288
LK+A ++W + P+ SIT ++++ K +F + ++ N Q E+L E
Sbjct: 448 LKEATQQWYMNLPRFSITGYQNMTHKLVHQFSASKHCKVSTTNLFNVRQDPNESLPE 618
>TC88051 similar to GP|15982866|gb|AAL09780.1 At1g27210/T7N9_27 {Arabidopsis
thaliana}, partial (16%)
Length = 1597
Score = 30.4 bits (67), Expect = 2.9
Identities = 22/93 (23%), Positives = 39/93 (41%), Gaps = 1/93 (1%)
Frame = +2
Query: 492 PQGKFSP-NALILSKHENADDVTTRSGRVLHESKKQVEGEKNDKVEEKDEGVIKKRVSEP 550
P +FSP ++ S +N +++ R L S+K + + +V D+G +++R
Sbjct: 209 PVNRFSPPDSSTASNGKNVSSLSSNKRRSLGVSRKLNQKNWDVQVAMADQGELQERDENG 388
Query: 551 VKARVLSTPSPELSKIPFPKALANKNLDKQFSK 583
K +AL NKN D + K
Sbjct: 389 TPLETSKVDKSRFLKPEMNRALLNKNSDDRIKK 487
>TC88456 similar to GP|15487292|dbj|BAB64531. vacuolar sorting receptor
{Vigna mungo}, partial (41%)
Length = 980
Score = 30.4 bits (67), Expect = 2.9
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = +2
Query: 563 LSKIPFPKALANKNLDKQFSKFLEVFKKLHINIPFSEAL 601
+ KI P AL +K+L + K L + +HIN+ + EAL
Sbjct: 596 IEKINIPSALISKSLGDRIKKALSDGEMVHINLDWREAL 712
>TC76698 similar to PIR|S50766|S50766 dehydrin-related protein - garden pea,
partial (80%)
Length = 974
Score = 30.4 bits (67), Expect = 2.9
Identities = 45/193 (23%), Positives = 73/193 (37%)
Frame = +2
Query: 353 AMNSENDRQTRRRMFEFEAYDKLLASNKQISEKVAEIQNHIKVTKLVGASVAKVECVTRG 412
A NSE R +F+F K +K E +A NH KVT S KVE G
Sbjct: 125 ATNSETTEIKDRGVFDFLGGKKKDEEHKPQEEAIATDFNH-KVTLYEAPSETKVEEKEEG 301
Query: 413 GPNFSNSCTMTNQGFQGQSSGLFQQVQEQVDVKDGGKKSLEELLESFINRMESNYKIQDA 472
++ ++ SS +E+VD + KK E + E +
Sbjct: 302 EKKHTSLLEKLHRSDSSSSS----SSEEEVDGEKRKKKKKE--------KKEDTSVAVEK 445
Query: 473 AIKNLKSQFGQLAKQMAKIPQGKFSPNALILSKHENADDVTTRSGRVLHESKKQVEGEKN 532
+ + G L K K+P H+ DDVTT + + + +
Sbjct: 446 VDGTTEEKKGFLDKIKEKLP------------GHKKTDDVTTPPAPPVVVAPAETTTTSH 589
Query: 533 DKVEEKDEGVIKK 545
D+ ++K +G+++K
Sbjct: 590 DQGDQK-KGILEK 625
>AW981373 similar to GP|8096650|dbj| ESTs C19814(E10971) AU090481(E10971)
correspond to a region of the predicted gene.~Similar
to, partial (16%)
Length = 346
Score = 29.6 bits (65), Expect = 4.9
Identities = 18/59 (30%), Positives = 28/59 (46%)
Frame = +3
Query: 529 GEKNDKVEEKDEGVIKKRVSEPVKARVLSTPSPELSKIPFPKALANKNLDKQFSKFLEV 587
GE+ + V G+ + R+ + VK +PEL I L N N+DK K L++
Sbjct: 138 GEEYEIVMTTPTGLYRYRLGDVVKVMGFHNSTPELKFIRRSSLLLNINIDKNTEKDLQL 314
>TC77822 weakly similar to GP|21536834|gb|AAM61166.1 unknown {Arabidopsis
thaliana}, partial (33%)
Length = 2391
Score = 29.3 bits (64), Expect = 6.4
Identities = 26/110 (23%), Positives = 48/110 (43%), Gaps = 2/110 (1%)
Frame = +2
Query: 613 DILSKKRKLSEVD--ETVMLTEECSAILQRKIPRKRRDPGSFTIPVEIEGLSKVDALCDL 670
D L K+ L E T + +E +L + R P F +P+ L ++ C +
Sbjct: 1835 DCLMKEFGLREASFMPTNKVKDEEQTMLYQMDKYDFRIPNMFLVPMV--ALIMINISCFI 2008
Query: 671 GASINLMPLSMFRRLNLGEVTPTMLSLQMADRSLKTPYGIIEDVMVKVDK 720
G ++R L+LGE+ + + + + Y IIE ++++ DK
Sbjct: 2009 GG--------IYRVLSLGELDKMFIQIYLMAHIILVNYPIIEGIVIRKDK 2134
>TC81220
Length = 985
Score = 29.3 bits (64), Expect = 6.4
Identities = 23/66 (34%), Positives = 34/66 (50%)
Frame = +3
Query: 570 KALANKNLDKQFSKFLEVFKKLHINIPFSEALEQMLIYAKFMRDILSKKRKLSEVDETVM 629
KAL +KN + S FL I++PF E+LE + + IL++K V TV+
Sbjct: 402 KALTHKNTNTLSSTFLP*LFDFLISLPFGESLETFQAFTE--GGILTRK----GVVVTVL 563
Query: 630 LTEECS 635
+TE S
Sbjct: 564 ITERLS 581
>TC89479 similar to GP|22136170|gb|AAM91163.1 unknown protein {Arabidopsis
thaliana}, partial (16%)
Length = 1759
Score = 28.9 bits (63), Expect = 8.4
Identities = 31/132 (23%), Positives = 60/132 (44%), Gaps = 14/132 (10%)
Frame = +2
Query: 438 VQEQVDVKDGGKKSLEELLESFINRMESNYKIQDAAIKN-LKSQFG---QLAKQMAKIPQ 493
VQ + DVK EE SF+ + SN +I DA + +++ G Q+ K + K+
Sbjct: 740 VQIEKDVKKVHDIPFEESNTSFVLKGNSNSEIVDADREEIIEADIGEIVQIEKNVKKVND 919
Query: 494 GKFS--PNALILSKHENADDVTTRSGRVLH---ESKKQVEGEKNDKVE-----EKDEGVI 543
+++L H+N + V S + + E Q+E + N + + + GV+
Sbjct: 920 ISVEEVDTSIVLKGHKNCEIVDANSEKTVEANIEDMVQIEKDANKEHDISIEYDNIRGVL 1099
Query: 544 KKRVSEPVKARV 555
K++ + + + V
Sbjct: 1100KRKSNVYISSNV 1135
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.139 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,948,272
Number of Sequences: 36976
Number of extensions: 293146
Number of successful extensions: 1986
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1465
Number of HSP's successfully gapped in prelim test: 75
Number of HSP's that attempted gapping in prelim test: 493
Number of HSP's gapped (non-prelim): 1574
length of query: 822
length of database: 9,014,727
effective HSP length: 104
effective length of query: 718
effective length of database: 5,169,223
effective search space: 3711502114
effective search space used: 3711502114
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0265.4