BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0264.1
(148 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81338 weakly similar to PIR|B86326|B86326 hypothetical protein... 189 4e-49
TC90079 similar to GP|5454204|gb|AAD43619.1| T3P18.18 {Arabidops... 76 4e-15
BF649223 weakly similar to GP|21740857|emb OSJNBb0072N21.6 {Oryz... 74 3e-14
BF643468 weakly similar to PIR|F96682|F96 hypothetical protein F... 72 8e-14
TC91882 similar to GP|10176714|dbj|BAB09944. dimethylaniline mon... 70 3e-13
AW585176 similar to GP|14532474|gb| At1g62600/T3P18_16 {Arabidop... 50 4e-07
TC87682 weakly similar to PIR|T04527|T04527 hypothetical protein... 42 7e-05
TC90236 similar to GP|4586308|dbj|BAA76348.1 protoporphyrinogen ... 40 5e-04
TC77553 homologue to GP|1066499|gb|AAB41904.1| NADH-dependent gl... 37 0.003
CB892762 weakly similar to GP|21536917|gb| putative glucosyltran... 33 0.032
TC86967 homologue to SP|Q43621|GSHR_PEA Glutathione reductase c... 31 0.16
TC78514 homologue to SP|Q9SMJ3|ZDS_CAPAN Zeta-carotene desaturas... 30 0.27
TC87617 similar to PIR|D86354|D86354 hypothetical protein AAF878... 30 0.47
TC86790 similar to PIR|A84861|A84861 probable amine oxidase [imp... 30 0.47
TC85753 similar to PIR|D96682|D96682 protein F1E22.18 [imported]... 30 0.47
TC81622 weakly similar to PIR|T00584|T00584 indole-3-acetate bet... 29 0.80
BI268054 weakly similar to GP|10177560|db glucuronosyl transfera... 28 1.4
TC78409 homologue to GP|18086418|gb|AAL57665.1 AT3g59050/F17J16_... 27 2.3
TC79558 weakly similar to PIR|T45743|T45743 hypothetical protein... 27 2.3
TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.... 27 4.0
>TC81338 weakly similar to PIR|B86326|B86326 hypothetical protein AAF82235.1
[imported] - Arabidopsis thaliana, partial (32%)
Length = 784
Score = 189 bits (480), Expect = 4e-49
Identities = 88/149 (59%), Positives = 117/149 (78%), Gaps = 3/149 (2%)
Frame = +2
Query: 3 REHNTMFVSKIAIIGAGVSGIAAAKQLSHHNPIIFEASDSIGGVWRHCSYNSTKLQSHRR 62
+++ VS+I IIGAGVSG+A AKQLSH+NPI+FEA+DSIGGVWRHCSY TKLQS
Sbjct: 41 KDNPPSIVSRIGIIGAGVSGLAVAKQLSHYNPIVFEATDSIGGVWRHCSYRCTKLQSQTW 220
Query: 63 DYEFTDFPWHERDNSDFPTHLEILKYLHSYAQHFDVMKNIRFNSKVVEIRFLGNRVPTEG 122
+YEF+DFPW ER++ D+P+H EIL+YLH YA +FD+ K ++FN+KVVEI+F+ ++ +
Sbjct: 221 NYEFSDFPWPERESKDYPSHAEILEYLHLYAVYFDLFKYVKFNTKVVEIKFVRDKEGFDF 400
Query: 123 ER---DFGSPLPGRPVWEVAVRDNHSDTI 148
R D G+PLP RPVWE++V+ N SD I
Sbjct: 401 GRLPGDHGNPLPERPVWELSVQTNESDAI 487
>TC90079 similar to GP|5454204|gb|AAD43619.1| T3P18.18 {Arabidopsis
thaliana}, partial (38%)
Length = 677
Score = 76.3 bits (186), Expect = 4e-15
Identities = 44/135 (32%), Positives = 71/135 (52%), Gaps = 25/135 (18%)
Frame = +3
Query: 8 MFVSKIAIIGAGVSGIAAAKQLSH--HNPIIFEASDSIGGVWRHCS-------------- 51
+F+ K+A+IGAGVSG+ AAK+L HN I+FE ++ +GG W + S
Sbjct: 6 VFMVKVAVIGAGVSGMVAAKELQQEGHNVIVFEKNNRVGGTWIYTSKSDSDPLSIDPTRE 185
Query: 52 ------YNSTKLQSHRRDYEFTDFPWHERDNSD---FPTHLEILKYLHSYAQHFDVMKNI 102
Y S + R F D+P +R++ D FP H E+L++L +A F + +
Sbjct: 186 TVHSSVYLSLRTNLPRHIMGFLDYPLSKRESGDPRTFPGHEEVLRFLEEFADEFGIHELT 365
Query: 103 RFNSKVVEIRFLGNR 117
RF ++VV++ G +
Sbjct: 366 RFETEVVKVERKGGK 410
>BF649223 weakly similar to GP|21740857|emb OSJNBb0072N21.6 {Oryza sativa},
partial (11%)
Length = 278
Score = 73.6 bits (179), Expect = 3e-14
Identities = 36/87 (41%), Positives = 55/87 (62%), Gaps = 10/87 (11%)
Frame = +2
Query: 2 IREHNTMFVS--------KIAIIGAGVSGIAAAKQLSH--HNPIIFEASDSIGGVWRHCS 51
IR +N + +S ++AI+GAG+SG+ A K ++ NPI+FEA D +GG+WRH +
Sbjct: 20 IRANNNLAISLIIYKMEKRVAIVGAGISGLLACKYVTQIGFNPIVFEADDGVGGLWRH-T 196
Query: 52 YNSTKLQSHRRDYEFTDFPWHERDNSD 78
STKLQ+ ++ Y+F+ FPW D
Sbjct: 197 IESTKLQNPKQAYQFSXFPWDSSXKED 277
>BF643468 weakly similar to PIR|F96682|F96 hypothetical protein F12P19.2
[imported] - Arabidopsis thaliana, partial (33%)
Length = 655
Score = 72.0 bits (175), Expect = 8e-14
Identities = 40/125 (32%), Positives = 67/125 (53%), Gaps = 25/125 (20%)
Frame = +2
Query: 12 KIAIIGAGVSGIAAAKQLSH--HNPIIFEASDSIGGVWRHCS------------------ 51
K+A+IGAGVSG+ AAK+L HN ++FE ++ +GG W +
Sbjct: 86 KVAVIGAGVSGLVAAKELQQEGHNVVVFEKNNRVGGTWIYTPKTDSDPLSMDPARETVHS 265
Query: 52 --YNSTKLQSHRRDYEFTDFPWHERDNSD---FPTHLEILKYLHSYAQHFDVMKNIRFNS 106
Y+S + R+ F D+P +R++ D FP H E+L++L +A F + + RF +
Sbjct: 266 SVYHSLRTNLPRQIMGFLDYPLSKRESGDPRTFPGHEEVLRFLEEFAGEFGIHELTRFET 445
Query: 107 KVVEI 111
+VV++
Sbjct: 446 EVVKV 460
>TC91882 similar to GP|10176714|dbj|BAB09944. dimethylaniline
monooxygenase-like protein {Arabidopsis thaliana},
partial (47%)
Length = 800
Score = 70.1 bits (170), Expect = 3e-13
Identities = 45/134 (33%), Positives = 65/134 (47%), Gaps = 26/134 (19%)
Frame = +2
Query: 1 MIREHNTMFVSKIAIIGAGVSGIAAAKQLSH--HNPIIFEASDSIGGVWR---------- 48
M+ E N + +IGAG +G+ AA+++ H I+ E + IGG W
Sbjct: 26 MVSETNQYQSKNVCVIGAGPAGLVAAREIRKEGHKVIVLEQNHDIGGQWLYDDKNIEGED 205
Query: 49 ----------HCS-YNSTKLQSHRRDYEFTDFPWHERDNSD---FPTHLEILKYLHSYAQ 94
H S YNS + QS R FTDFP+ + D FP H+EIL YL + +
Sbjct: 206 PLGRNPFLKVHSSIYNSLRTQSPREVMGFTDFPFSTKKGRDMRRFPGHVEILMYLKDFCE 385
Query: 95 HFDVMKNIRFNSKV 108
F + + IRFN++V
Sbjct: 386 WFGLREMIRFNTRV 427
>AW585176 similar to GP|14532474|gb| At1g62600/T3P18_16 {Arabidopsis
thaliana}, partial (21%)
Length = 476
Score = 49.7 bits (117), Expect = 4e-07
Identities = 30/103 (29%), Positives = 46/103 (44%), Gaps = 27/103 (26%)
Frame = +3
Query: 13 IAIIGAGVSGIAAAKQLSH--HNPIIFEASDSIGGVWRHCS------------------- 51
+ IGAG G+ AA++L H ++FE + +GG W + S
Sbjct: 159 VFFIGAGAGGLVAARELRREGHQVVVFERGEELGGSWVYTSEVESDPLGLDPNRKLIHSS 338
Query: 52 -YNSTKLQSHRRDYEFTDFPWHE-----RDNSDFPTHLEILKY 88
YNS + R F D+P+ RD++ FP+H E+L Y
Sbjct: 339 LYNSLRTNLPRESMGFRDYPFRRKEEKGRDSTRFPSHGEVLMY 467
>TC87682 weakly similar to PIR|T04527|T04527 hypothetical protein F16A16.170
- Arabidopsis thaliana, partial (38%)
Length = 1040
Score = 42.4 bits (98), Expect = 7e-05
Identities = 26/96 (27%), Positives = 48/96 (49%), Gaps = 2/96 (2%)
Frame = +1
Query: 5 HNTMFVSKIAIIGAGVSGIAAAKQLSHHNP--IIFEASDSIGGVWRHCSYNSTKLQSHRR 62
+N M + + IIGAG SG+A A L+ + II E + +W++ +Y+ L ++
Sbjct: 19 NNRMHEAPVIIIGAGTSGLATAACLTKQSIPFIILERENCFASLWQNYTYDRVHLHLRKQ 198
Query: 63 DYEFTDFPWHERDNSDFPTHLEILKYLHSYAQHFDV 98
E FP+ P + ++YL +Y +F++
Sbjct: 199 LCELPHFPFPPSYPHYVPKK-QFIEYLGNYVNNFNI 303
>TC90236 similar to GP|4586308|dbj|BAA76348.1 protoporphyrinogen IX
oxidase {Glycine max}, partial (39%)
Length = 686
Score = 39.7 bits (91), Expect = 5e-04
Identities = 21/51 (41%), Positives = 31/51 (60%), Gaps = 2/51 (3%)
Frame = +1
Query: 3 REHNTMFVSKIAIIGAGVSGIAAAKQLSHH--NPIIFEASDSIGGVWRHCS 51
++ N V ++A++GAGVSG+AAA +L H + +FEA GG R S
Sbjct: 31 KDDNPRSVKRVAVVGAGVSGLAAAYKLKSHGLDVTVFEAEGRAGGRLRTVS 183
>TC77553 homologue to GP|1066499|gb|AAB41904.1| NADH-dependent glutamate
synthase {Medicago sativa}, partial (37%)
Length = 3067
Score = 37.0 bits (84), Expect = 0.003
Identities = 18/37 (48%), Positives = 27/37 (72%), Gaps = 2/37 (5%)
Frame = +1
Query: 12 KIAIIGAGVSGIAAAKQLSH--HNPIIFEASDSIGGV 46
++AI+G+G SG+AAA QL+ H +FE +D IGG+
Sbjct: 1378 RVAIVGSGPSGLAAADQLNKMGHIVTVFERADRIGGL 1488
>CB892762 weakly similar to GP|21536917|gb| putative glucosyltransferase
{Arabidopsis thaliana}, partial (15%)
Length = 572
Score = 33.5 bits (75), Expect = 0.032
Identities = 16/35 (45%), Positives = 19/35 (53%), Gaps = 8/35 (22%)
Frame = +2
Query: 30 SHHNPIIFEASD--------SIGGVWRHCSYNSTK 56
+HH +I E D SIGG W HC +NSTK
Sbjct: 8 AHHMGLIMEWCDQLRVLSHPSIGGFWSHCGWNSTK 112
>TC86967 homologue to SP|Q43621|GSHR_PEA Glutathione reductase cytosolic
(EC 1.6.4.2) (GR) (GRASE) (GOR2). [Garden pea] {Pisum
sativum}, complete
Length = 1828
Score = 31.2 bits (69), Expect = 0.16
Identities = 27/92 (29%), Positives = 39/92 (42%), Gaps = 16/92 (17%)
Frame = +3
Query: 4 EHNTMFVSKIAIIGAGVSGIAAAKQLSHHN--------PIIFEASDSIGGVWRHCSYNS- 54
E T F + IIGAG G+ AA+ S+H P +S++IGGV C
Sbjct: 192 EEGTNFDFDLFIIGAGSGGVRAARFSSNHGAKVAICELPFHPISSETIGGVGGTCVIRGC 371
Query: 55 --TKLQSHRRDY-----EFTDFPWHERDNSDF 79
K+ + Y + +F W +N DF
Sbjct: 372 VPKKILVYGASYGGDLEDARNFGWELSENIDF 467
>TC78514 homologue to SP|Q9SMJ3|ZDS_CAPAN Zeta-carotene desaturase
chloroplast precursor (EC 1.14.99.30) (Carotene 7
8-desaturase)., partial (88%)
Length = 2063
Score = 30.4 bits (67), Expect = 0.27
Identities = 16/36 (44%), Positives = 24/36 (66%), Gaps = 2/36 (5%)
Frame = +3
Query: 12 KIAIIGAGVSGIAAAKQL--SHHNPIIFEASDSIGG 45
K+AIIGAG++G++ A +L H I+E+ IGG
Sbjct: 393 KVAIIGAGLAGMSTAVELLDQGHEVDIYESRTFIGG 500
>TC87617 similar to PIR|D86354|D86354 hypothetical protein AAF87855.1
[imported] - Arabidopsis thaliana, partial (77%)
Length = 1222
Score = 29.6 bits (65), Expect = 0.47
Identities = 14/38 (36%), Positives = 20/38 (51%)
Frame = -1
Query: 60 HRRDYEFTDFPWHERDNSDFPTHLEILKYLHSYAQHFD 97
H R+ F DF W + P + +L Y+ S+A HFD
Sbjct: 736 HHREEWFKDFRWIVQYCQTQPFRIMLL*YMSSFACHFD 623
>TC86790 similar to PIR|A84861|A84861 probable amine oxidase [imported] -
Arabidopsis thaliana, partial (91%)
Length = 2198
Score = 29.6 bits (65), Expect = 0.47
Identities = 14/35 (40%), Positives = 23/35 (65%), Gaps = 2/35 (5%)
Frame = +2
Query: 13 IAIIGAGVSGIAAAKQL--SHHNPIIFEASDSIGG 45
+ +IG G++GIAAA+ L + ++ E+ D IGG
Sbjct: 467 VIVIGGGMAGIAAARALHDASFQVVLLESRDRIGG 571
>TC85753 similar to PIR|D96682|D96682 protein F1E22.18 [imported] -
Arabidopsis thaliana, partial (85%)
Length = 2024
Score = 29.6 bits (65), Expect = 0.47
Identities = 14/35 (40%), Positives = 23/35 (65%), Gaps = 2/35 (5%)
Frame = +1
Query: 13 IAIIGAGVSGIAAAKQL--SHHNPIIFEASDSIGG 45
+ ++GAG+SGIAAA+ L + + E+ D +GG
Sbjct: 469 VIVVGAGISGIAAARILHDASFKVTLLESRDRLGG 573
>TC81622 weakly similar to PIR|T00584|T00584 indole-3-acetate
beta-glucosyltransferase homolog T27E13.12 - Arabidopsis
thaliana, partial (25%)
Length = 1735
Score = 28.9 bits (63), Expect = 0.80
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2
Query: 42 SIGGVWRHCSYNST 55
SIGG W HC +NST
Sbjct: 1142 SIGGFWSHCGWNST 1183
>BI268054 weakly similar to GP|10177560|db glucuronosyl transferase-like
protein {Arabidopsis thaliana}, partial (21%)
Length = 650
Score = 28.1 bits (61), Expect = 1.4
Identities = 9/17 (52%), Positives = 12/17 (69%)
Frame = +3
Query: 39 ASDSIGGVWRHCSYNST 55
A ++GG W HC +NST
Sbjct: 543 AHKAVGGFWSHCGWNST 593
>TC78409 homologue to GP|18086418|gb|AAL57665.1 AT3g59050/F17J16_100
{Arabidopsis thaliana}, partial (19%)
Length = 689
Score = 27.3 bits (59), Expect = 2.3
Identities = 12/35 (34%), Positives = 23/35 (65%), Gaps = 2/35 (5%)
Frame = +1
Query: 13 IAIIGAGVSGIAAAKQL--SHHNPIIFEASDSIGG 45
+ +IG G++GIAAA+ L + ++ E+ + +GG
Sbjct: 406 VIVIGGGMAGIAAARALQDASFQVVLLESRERLGG 510
>TC79558 weakly similar to PIR|T45743|T45743 hypothetical protein F24M12.160 -
Arabidopsis thaliana, partial (25%)
Length = 1793
Score = 27.3 bits (59), Expect = 2.3
Identities = 19/72 (26%), Positives = 31/72 (42%)
Frame = +1
Query: 7 TMFVSKIAIIGAGVSGIAAAKQLSHHNPIIFEASDSIGGVWRHCSYNSTKLQSHRRDYEF 66
+M K+ G + GI A++ + S+ WR CSY ++ ++ RR +E
Sbjct: 1120 SMIYWKLRNCGLIIFGIEQARR---------DTEKSLKNAWRSCSYLNSPVERQRRLHEV 1272
Query: 67 TDFPWHERDNSD 78
D H N D
Sbjct: 1273 PDV--HSDPNLD 1302
>TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.-.-.-) 2 -
Arabidopsis thaliana, partial (61%)
Length = 1557
Score = 26.6 bits (57), Expect = 4.0
Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 8/45 (17%)
Frame = +2
Query: 13 IAIIGAGVSGIAAAKQLSH--HNPIIFEASDSIG------GVWRH 49
+ I+GAG++G+A A L ++ E+SDS+ G+W +
Sbjct: 125 VVIVGAGIAGLATALGLHRLGVRSLVLESSDSLRVTGFALGIWEN 259
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.137 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,157,305
Number of Sequences: 36976
Number of extensions: 72666
Number of successful extensions: 456
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 446
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 448
length of query: 148
length of database: 9,014,727
effective HSP length: 87
effective length of query: 61
effective length of database: 5,797,815
effective search space: 353666715
effective search space used: 353666715
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0264.1