BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0262.8
(312 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90837 weakly similar to GP|22202752|dbj|BAC07409. hypothetical... 108 2e-24
BI271381 similar to GP|21397269|gb Unknown protein {Oryza sativa... 44 6e-07
BG647163 similar to GP|9757943|dbj emb|CAB72466.1~gene_id:MJC20.... 40 9e-04
BG587167 similar to PIR|T47439|T474 hypothetical protein T18B22.... 39 0.002
AL374958 GP|8979817|emb| dJ447E21.2 (Similar to X.laevis Xrel2 p... 36 0.022
AW560293 weakly similar to GP|21902022|db contains EST D24834(R2... 35 0.037
BF646282 weakly similar to GP|17104745|gb| unknown protein {Arab... 33 0.11
TC77062 similar to GP|20259307|gb|AAM14389.1 putative nodulin pr... 32 0.31
AW267858 weakly similar to GP|21592524|gb unknown {Arabidopsis t... 31 0.54
TC91039 similar to PIR|C96640|C96640 hypothetical protein T25B24... 30 1.2
TC85575 similar to PIR|D86410|D86410 protein F3M18.16 [imported]... 30 1.2
TC87209 similar to GP|9663767|emb|CAC01237.1 RNA Binding Protein... 30 1.6
TC93312 GP|15292335|gb|AAK93436.1 LD47289p {Drosophila melanogas... 28 4.5
AW257224 28 4.5
TC91240 homologue to PIR|T48518|T48518 transcription factor like... 28 5.9
TC88785 similar to GP|15144509|gb|AAK84476.1 unknown {Lycopersic... 27 7.7
>TC90837 weakly similar to GP|22202752|dbj|BAC07409. hypothetical
protein~similar to Oryza sativa chromosome10
OSJNBa0079H13.17, partial (7%)
Length = 596
Score = 108 bits (271), Expect = 2e-24
Identities = 54/103 (52%), Positives = 66/103 (63%)
Frame = +1
Query: 164 GKYYLVDAGYPTFMGFLGPYKKNRYHLPQFRNGPRITGRVEVFNYYHSSLRNVIERSFGC 223
G+YYLVD GYP G++ PY + RYH QF + P T E FN HSSLR+ IERSFG
Sbjct: 4 GRYYLVDKGYPDKEGYMVPYPRIRYHQSQFEHEPP-TNAQEAFNRAHSSLRSCIERSFGV 180
Query: 224 CKAKWKILGSMPSYDLKTQNKIITACMALHNFIRRNDRSDEEF 266
K +WKIL MP + +KTQ +I A ALHN+IR N + D F
Sbjct: 181 LKKRWKILNKMPQFSVKTQIDVIIAAFALHNYIRINSQDDAMF 309
>BI271381 similar to GP|21397269|gb Unknown protein {Oryza sativa (japonica
cultivar-group)}, partial (2%)
Length = 407
Score = 44.3 bits (103), Expect(2) = 6e-07
Identities = 23/51 (45%), Positives = 32/51 (62%)
Frame = +2
Query: 204 EVFNYYHSSLRNVIERSFGCCKAKWKILGSMPSYDLKTQNKIITACMALHN 254
E+FNY HSSLR IER FG K ++ IL MPSY K + ++ C+ ++
Sbjct: 140 ELFNYRHSSLRMTIERCFGVLKNRFPILKLMPSY--KPSRQRLSNCLLCYS 286
Score = 26.2 bits (56), Expect(2) = 6e-07
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = +3
Query: 246 ITACMALHNFIRRNDRSDEEFDANYENED 274
+TAC A+HN+I + + DE F E +D
Sbjct: 264 VTACCAIHNYICKWNLPDELFRIWEEMDD 350
>BG647163 similar to GP|9757943|dbj emb|CAB72466.1~gene_id:MJC20.8~similar to
unknown protein {Arabidopsis thaliana}, partial (51%)
Length = 744
Score = 40.4 bits (93), Expect = 9e-04
Identities = 16/33 (48%), Positives = 20/33 (60%)
Frame = +3
Query: 164 GKYYLVDAGYPTFMGFLGPYKKNRYHLPQFRNG 196
GKYYLVD +P GF+ PY + YH +F G
Sbjct: 618 GKYYLVDNKFPNVPGFIAPYPRTPYHSKEFPTG 716
>BG587167 similar to PIR|T47439|T474 hypothetical protein T18B22.40 -
Arabidopsis thaliana, partial (11%)
Length = 788
Score = 39.3 bits (90), Expect = 0.002
Identities = 19/50 (38%), Positives = 31/50 (62%)
Frame = +2
Query: 225 KAKWKILGSMPSYDLKTQNKIITACMALHNFIRRNDRSDEEFDANYENED 274
K+ + I S P + KT+ +I+ AC+ LHNF+R+ R+D F ++ED
Sbjct: 515 KSLFLIFKSAPPFPYKTKKEIVLACVGLHNFLRKFCRTD-NFPEEKDSED 661
>AL374958 GP|8979817|emb| dJ447E21.2 (Similar to X.laevis Xrel2 protein)
{Homo sapiens}, partial (73%)
Length = 524
Score = 35.8 bits (81), Expect = 0.022
Identities = 20/38 (52%), Positives = 23/38 (59%)
Frame = +3
Query: 230 ILGSMPSYDLKTQNKIITACMALHNFIRRNDRSDEEFD 267
IL M + L Q KII A +ALHNFIR DEEF+
Sbjct: 15 ILCDMRPFPLIKQEKIIVATIALHNFIRICGVQDEEFN 128
>AW560293 weakly similar to GP|21902022|db contains EST D24834(R2633)~similar
to Arabidopsis thaliana chromosome 5 At5g12010~unknown
protein, partial (25%)
Length = 623
Score = 35.0 bits (79), Expect = 0.037
Identities = 26/130 (20%), Positives = 52/130 (40%), Gaps = 1/130 (0%)
Frame = -2
Query: 151 TLMY*HAIIFYCLGKYYLVDAGYPTFMGFLGPYKKNRYHLPQFRNGPRITGRVEVFNYYH 210
++MY A+ + + ++G+P G L PY +T FN
Sbjct: 589 SVMYQRAMTGNLKDSWVVGNSGFPLMDGILVPYTHQN-----------LTWTQHAFNEKV 443
Query: 211 SSLRNVIERSFGCCKAKWKILGSMPSYDLKTQNKIITACMALHNFIR-RNDRSDEEFDAN 269
++ + + +F K +W L ++ ++ AC LHN RN++ D ++
Sbjct: 442 EDIQKLSKDAFAKVKGRWSCLQKRIEVKIEELPGVLGACCVLHNICEMRNEKMDPAWNFE 263
Query: 270 YENEDGGGEN 279
+++ EN
Sbjct: 262 LFDDEMVAEN 233
>BF646282 weakly similar to GP|17104745|gb| unknown protein {Arabidopsis
thaliana}, partial (15%)
Length = 570
Score = 33.5 bits (75), Expect = 0.11
Identities = 24/93 (25%), Positives = 40/93 (42%), Gaps = 1/93 (1%)
Frame = +1
Query: 165 KYYLVDAGYPTFMGFLGPYKKNRYHLPQFRNGPRITGRVEVFNYYHSSLRNVIERSFGCC 224
+Y + D G+P L PY+ G ++ FN ++ + V +R+
Sbjct: 1 EYIVGDTGFPLLPWLLTPYE-----------GEGLSNVQVDFNKRVAATQMVAKRALSRL 147
Query: 225 KAKWKILGSMPSYDLKTQ-NKIITACMALHNFI 256
K WKI+G + K + +II C LHN +
Sbjct: 148 KEMWKIIGGVMQKPNKHKLPRIILVCCILHNIV 246
>TC77062 similar to GP|20259307|gb|AAM14389.1 putative nodulin protein
{Arabidopsis thaliana}, partial (81%)
Length = 1631
Score = 32.0 bits (71), Expect = 0.31
Identities = 18/46 (39%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = +1
Query: 89 TLPLMSWLL-VISTCVSPLSGLGGRVLHMMLRFLWRLCVDHHCIFP 133
TL +M WL V STC +SG + H + FLW + H+C P
Sbjct: 769 TLFVMVWLAGVASTCP*EVSG--SPLCHFLYMFLWTYPISHYCFDP 900
>AW267858 weakly similar to GP|21592524|gb unknown {Arabidopsis thaliana},
partial (21%)
Length = 556
Score = 31.2 bits (69), Expect = 0.54
Identities = 27/116 (23%), Positives = 43/116 (36%), Gaps = 6/116 (5%)
Frame = +1
Query: 166 YYLVDAGYPTFMGFLGPYKKNRYHLPQFRNGPRITGRVEVFNYYHSSLRNVIERSFGCCK 225
+ ++++GY L PYK +T FN + + + +F K
Sbjct: 46 WIVLNSGYSLMDWVLVPYKHQN-----------LTWTQHGFNERIGEIEKIGKDAFSRLK 192
Query: 226 AKWKILGSMPSYDLKTQNKIITACMALHNFIR-RNDRSDEE-----FDANYENEDG 275
+W L L+ ++ AC LHN N+ D+E FD EDG
Sbjct: 193 GRWGCLKKRTEIKLQDLPVVLGACCVLHNICEIMNEEMDDEWKFEVFDDEMVVEDG 360
>TC91039 similar to PIR|C96640|C96640 hypothetical protein T25B24.14
[imported] - Arabidopsis thaliana, partial (4%)
Length = 732
Score = 30.0 bits (66), Expect = 1.2
Identities = 21/45 (46%), Positives = 25/45 (54%)
Frame = +2
Query: 51 MMSDITLILKIALVQ*MVLT*EFVFLLNSRQLILVGKVTLPLMSW 95
MMS LI +I L MVL * VFL + +I V K + P MSW
Sbjct: 593 MMSGTCLISRIVLDVLMVLI*LHVFLRRIKCVIEVEKESPPSMSW 727
>TC85575 similar to PIR|D86410|D86410 protein F3M18.16 [imported] -
Arabidopsis thaliana, partial (15%)
Length = 1225
Score = 30.0 bits (66), Expect = 1.2
Identities = 30/128 (23%), Positives = 59/128 (45%), Gaps = 8/128 (6%)
Frame = -3
Query: 43 VMFLMRLRMM--------SDITLILKIALVQ*MVLT*EFVFLLNSRQLILVGKVTLPLMS 94
V+FL+R+ ++ S +T L + +V +V+T E VF++NS +++V
Sbjct: 950 VIFLLRVNIIKIFSTFHNSRVTHSLPLTIVV-IVVTFEAVFVINSNFVVVV--------- 801
Query: 95 WLLVISTCVSPLSGLGGRVLHMMLRFLWRLCVDHHCIFPILLMVRNI*NL*LYNIVTLMY 154
LLV+ + +LH ++ F W LCV +++ + L +V ++
Sbjct: 800 ILLVVVVVL---------ILH*VVTFFWELCVARRIFVVVVVEI-------LVVVVVPVF 669
Query: 155 *HAIIFYC 162
+ F+C
Sbjct: 668 HGVVSFFC 645
>TC87209 similar to GP|9663767|emb|CAC01237.1 RNA Binding Protein 45
{Nicotiana plumbaginifolia}, partial (69%)
Length = 1504
Score = 29.6 bits (65), Expect = 1.6
Identities = 15/43 (34%), Positives = 22/43 (50%)
Frame = +3
Query: 268 ANYENEDGGGENEAGPSTVTWEEPDFQSSLQMEQIREHIKNMF 310
A+Y+N GG +NE P+ T F +L EH+K +F
Sbjct: 804 ASYQNPQGGAQNENDPNNTT----IFVGNLDPNVTDEHLKQVF 920
>TC93312 GP|15292335|gb|AAK93436.1 LD47289p {Drosophila melanogaster},
partial (2%)
Length = 493
Score = 28.1 bits (61), Expect = 4.5
Identities = 19/79 (24%), Positives = 37/79 (46%), Gaps = 11/79 (13%)
Frame = +3
Query: 244 KIITACMALHNFIRRNDRSDEEFDANYENEDGGGENEAGPST-----------VTWEEPD 292
K++T+ + + +D D+ F ED GGEN+ GPS+ +T+ E D
Sbjct: 204 KLVTSPIPRFKLLDSDDDDDDLF-----GEDVGGENKVGPSSSSGQTCNRNTPLTYSEQD 368
Query: 293 FQSSLQMEQIREHIKNMFP 311
++ + + ++ K+ P
Sbjct: 369 RKTQFDVNRNQDLWKDFSP 425
>AW257224
Length = 646
Score = 28.1 bits (61), Expect = 4.5
Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Frame = +3
Query: 234 MPSYDLKTQNKIITACMALHNFIR-RNDRSDEEFDANYENEDGGGENE 280
+PS L K+ + + I +D D E D NYEN+DG N+
Sbjct: 480 LPSMSLDDYFKMNNLLLEVEGDINSESDNGDVEGDINYENDDGEKTND 623
>TC91240 homologue to PIR|T48518|T48518 transcription factor like protein -
Arabidopsis thaliana, partial (7%)
Length = 680
Score = 27.7 bits (60), Expect = 5.9
Identities = 10/31 (32%), Positives = 20/31 (64%)
Frame = +2
Query: 197 PRITGRVEVFNYYHSSLRNVIERSFGCCKAK 227
P + V +FN HS ++ + R++GCC+++
Sbjct: 131 PPTSKPVAIFNDQHSRFKH*VGRNWGCCESE 223
>TC88785 similar to GP|15144509|gb|AAK84476.1 unknown {Lycopersicon
esculentum}, partial (8%)
Length = 1417
Score = 27.3 bits (59), Expect = 7.7
Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 9/48 (18%)
Frame = +2
Query: 257 RRNDRSDEEFDANYENEDGGG------ENEAGPSTVTWEEP---DFQS 295
R R D E+D + DGGG E++ ++ W EP DFQS
Sbjct: 737 REERRVDREYDVVLVSSDGGGGCLSGSESDDSDWSIGWLEPHGSDFQS 880
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.342 0.152 0.496
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,731,014
Number of Sequences: 36976
Number of extensions: 167564
Number of successful extensions: 1413
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1402
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1411
length of query: 312
length of database: 9,014,727
effective HSP length: 96
effective length of query: 216
effective length of database: 5,465,031
effective search space: 1180446696
effective search space used: 1180446696
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (22.0 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0262.8