BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0252a.4
(153 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78326 weakly similar to GP|20465617|gb|AAM20140.1 unknown prot... 221 7e-59
TC90680 similar to GP|12836314|dbj|BAB23601. data source:SPTR s... 30 0.38
CA858222 similar to GP|11994369|dbj gb|AAD25600.1~gene_id:MDC16.... 29 0.84
AW694515 weakly similar to GP|10178181|dbj gene_id:MQN23.13~pir|... 27 3.2
TC80028 27 4.2
TC80269 weakly similar to GP|9294388|dbj|BAB02398.1 cytochrome P... 26 5.5
TC82818 similar to GP|20259203|gb|AAM14317.1 unknown protein {Ar... 26 5.5
BG449045 similar to GP|19310435|gb| F17H15.1/F17H15.1 {Arabidops... 26 5.5
TC80981 similar to GP|8698730|gb|AAF78488.1| ESTs gb|Z18526 gb|... 26 7.2
TC76851 homologue to PIR|S40122|S40122 high mobility group prote... 26 7.2
TC89926 similar to PIR|E96508|E96508 hypothetical protein T12C22... 26 7.2
TC77068 weakly similar to PIR|C96759|C96759 protein serine carbo... 26 7.2
TC88351 similar to GP|5262946|emb|CAB45873.1 beta-alanine syntha... 26 7.2
TC86293 homologue to GP|5305145|emb|CAB46084.1 fructose-1 6-bisp... 26 7.2
TC91382 GP|21327675|gb|AAC37438.2 CPH1 {Chlamydomonas reinhardti... 25 9.3
TC76935 similar to SP|P21747|EA92_VICFA Embryonic abundant prote... 25 9.3
TC87624 similar to GP|12321915|gb|AAG50994.1 unknown protein; 32... 25 9.3
AL389286 similar to GP|17862852|gb| RE01016p {Drosophila melanog... 25 9.3
>TC78326 weakly similar to GP|20465617|gb|AAM20140.1 unknown protein
{Arabidopsis thaliana}, partial (32%)
Length = 1590
Score = 221 bits (564), Expect = 7e-59
Identities = 111/158 (70%), Positives = 138/158 (87%), Gaps = 7/158 (4%)
Frame = +3
Query: 1 AIMYVLCFRMRSLVDIPRLKLQLLNMPMEPIWKHKLSPLKVCLPTVVEEFLRQAKAAQLF 60
AIMY+LCFRM+SL+++PRLK++L+ MPME IWKHKL+PLKVCLP+VVEEFL+QAKAA+LF
Sbjct: 678 AIMYILCFRMKSLMEVPRLKVKLIKMPMELIWKHKLNPLKVCLPSVVEEFLKQAKAARLF 857
Query: 61 MSSSETFVFNDLLESDDLSRSFGGIDRLDMFFPFDPCLSKKSESYIRPHFVRWSRVRTTY 120
M S+E F+F+DLLE+ DLS++FGGI+RLDMFFPFDPCL KKSESY+RPHFVRWS+VRTTY
Sbjct: 858 M-SAELFIFDDLLEA-DLSKAFGGINRLDMFFPFDPCLLKKSESYLRPHFVRWSKVRTTY 1031
Query: 121 DSDDDED----GSEMSHDDFVDRNAKEL---DMMVSVQ 151
D+ +E+ GSE S DDFVD NAK++ DM SV+
Sbjct: 1032DTYANEELSDSGSEASDDDFVDMNAKDMNDYDMAGSVE 1145
>TC90680 similar to GP|12836314|dbj|BAB23601. data source:SPTR source
key:Q9H0W3 evidence:ISS~homolog to HYPOTHETICAL 64.6
KDA PROTEIN~putative, partial (5%)
Length = 1280
Score = 30.0 bits (66), Expect = 0.38
Identities = 17/50 (34%), Positives = 26/50 (52%)
Frame = +2
Query: 96 PCLSKKSESYIRPHFVRWSRVRTTYDSDDDEDGSEMSHDDFVDRNAKELD 145
P S K + RP R + + +D D+D S MSH D++ + KE+D
Sbjct: 353 PTASLKRRRHPRPPQSRHNH----HKNDVDDDPSSMSHTDYILKRRKEVD 490
>CA858222 similar to GP|11994369|dbj gb|AAD25600.1~gene_id:MDC16.10~similar
to unknown protein {Arabidopsis thaliana}, partial (10%)
Length = 519
Score = 28.9 bits (63), Expect = 0.84
Identities = 20/79 (25%), Positives = 32/79 (40%)
Frame = +3
Query: 34 HKLSPLKVCLPTVVEEFLRQAKAAQLFMSSSETFVFNDLLESDDLSRSFGGIDRLDMFFP 93
H + K+C VE+ K+ SSS + S S++ G+ R F+P
Sbjct: 207 HNVKKAKMCYVGAVEDVSFDHKSKSP*FSSSGSSYSRRSCMSKTTSKANNGVKRSVRFYP 386
Query: 94 FDPCLSKKSESYIRPHFVR 112
L + SE +P +R
Sbjct: 387 VSVILGEDSEQQHQPSTIR 443
>AW694515 weakly similar to GP|10178181|dbj
gene_id:MQN23.13~pir||T07721~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (8%)
Length = 667
Score = 26.9 bits (58), Expect = 3.2
Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
Frame = -1
Query: 5 VLCFRMRSLVDIPRLK--LQLLNMPMEPIWKHKLSPLKVC 42
+LC R S I L+ LQL+ M + P W H +S L C
Sbjct: 250 LLC*RKSSFSSISSLE**LQLVLMILIPFWNHPISCLCFC 131
>TC80028
Length = 1120
Score = 26.6 bits (57), Expect = 4.2
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +1
Query: 97 CLSKKSESYIRPHFVRW 113
C + +SY+ PHFV+W
Sbjct: 1039 CWNASLKSYLHPHFVKW 1089
>TC80269 weakly similar to GP|9294388|dbj|BAB02398.1 cytochrome P450
{Arabidopsis thaliana}, partial (23%)
Length = 681
Score = 26.2 bits (56), Expect = 5.5
Identities = 16/43 (37%), Positives = 20/43 (46%)
Frame = -1
Query: 63 SSETFVFNDLLESDDLSRSFGGIDRLDMFFPFDPCLSKKSESY 105
SSE F+ DL L RSF ++ FP PC K S+
Sbjct: 288 SSERFMDFDLASFSILKRSFMSPSKILYEFP*KPCSLKSFSSF 160
>TC82818 similar to GP|20259203|gb|AAM14317.1 unknown protein {Arabidopsis
thaliana}, partial (43%)
Length = 1176
Score = 26.2 bits (56), Expect = 5.5
Identities = 23/77 (29%), Positives = 33/77 (41%), Gaps = 4/77 (5%)
Frame = +1
Query: 81 SFGGIDRLDMFFPFDPCLSKKSESYIRPHFVRWSR-VRTTYDSDDDEDG---SEMSHDDF 136
S+ +RL + P DP L + + +R + VR +Y D DED HD+F
Sbjct: 253 SYHNCNRLHL--PHDPALQRSLQIALRNRDDHDNDDVRRSYHDDHDEDDHDMETDDHDEF 426
Query: 137 VDRNAKELDMMVSVQGL 153
+ A D V GL
Sbjct: 427 EENYASHGDSRVIFGGL 477
>BG449045 similar to GP|19310435|gb| F17H15.1/F17H15.1 {Arabidopsis
thaliana}, partial (13%)
Length = 572
Score = 26.2 bits (56), Expect = 5.5
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +2
Query: 2 IMYVLCFRMRSLVDIPRLKLQLLNMPMEPIWKHKL 36
+M L R S+ +PRLKL LL++ + W H L
Sbjct: 464 LMLFLLLRNSSMKSLPRLKLGLLSVALXG-WLHNL 565
>TC80981 similar to GP|8698730|gb|AAF78488.1| ESTs gb|Z18526 gb|AI994480
gb|T44186 gb|Z30806 come from this gene. {Arabidopsis
thaliana}, partial (37%)
Length = 699
Score = 25.8 bits (55), Expect = 7.2
Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = +3
Query: 121 DSDDDEDGSEMSHD-DFVDRNAKELDM 146
DSDDD DG+ D DF D E+D+
Sbjct: 405 DSDDDSDGNVSGSDSDFSDDPLAEVDL 485
>TC76851 homologue to PIR|S40122|S40122 high mobility group protein HMG-1 -
garden pea, partial (92%)
Length = 863
Score = 25.8 bits (55), Expect = 7.2
Identities = 10/15 (66%), Positives = 11/15 (72%)
Frame = +2
Query: 121 DSDDDEDGSEMSHDD 135
+ DDDEDGSE DD
Sbjct: 515 NEDDDEDGSEEEEDD 559
>TC89926 similar to PIR|E96508|E96508 hypothetical protein T12C22.19
[imported] - Arabidopsis thaliana, partial (41%)
Length = 1556
Score = 25.8 bits (55), Expect = 7.2
Identities = 12/37 (32%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
Frame = +2
Query: 116 VRTTYDSDDDEDGSEMSHDDFVD--RNAKELDMMVSV 150
+R D +++EDG ++ +D+F+D + E D SV
Sbjct: 137 IRDEPDPEEEEDGEDLYNDNFLDDYQRMGEADQFESV 247
>TC77068 weakly similar to PIR|C96759|C96759 protein serine
carboxypeptidase T18K17.3 [imported] - Arabidopsis
thaliana, partial (50%)
Length = 998
Score = 25.8 bits (55), Expect = 7.2
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +1
Query: 41 VCLPTVVEEFLRQAKAAQLFMSSSETFVFNDLL 73
+CLPT+ E RQ K A+ F F+D L
Sbjct: 28 ICLPTLTFEMKRQRKGAE*HNK*MAKFRFHDFL 126
>TC88351 similar to GP|5262946|emb|CAB45873.1 beta-alanine synthase
{Lycopersicon esculentum}, partial (98%)
Length = 1255
Score = 25.8 bits (55), Expect = 7.2
Identities = 14/28 (50%), Positives = 17/28 (60%), Gaps = 2/28 (7%)
Frame = -3
Query: 39 LKVCLPTVV--EEFLRQAKAAQLFMSSS 64
L +CL V+ +EFL Q LFMSSS
Sbjct: 263 LSLCLTEVITFKEFLNQNNVCTLFMSSS 180
>TC86293 homologue to GP|5305145|emb|CAB46084.1 fructose-1 6-bisphosphatase
{Pisum sativum}, complete
Length = 1377
Score = 25.8 bits (55), Expect = 7.2
Identities = 13/37 (35%), Positives = 20/37 (53%), Gaps = 4/37 (10%)
Frame = +1
Query: 101 KSESYIRPHF----VRWSRVRTTYDSDDDEDGSEMSH 133
++ +YI F V W R+R + +D ED S+M H
Sbjct: 67 RTNTYISKEFGTYVVDWFRLRFIHWKEDFEDSSKMDH 177
>TC91382 GP|21327675|gb|AAC37438.2 CPH1 {Chlamydomonas reinhardtii}, partial
(1%)
Length = 702
Score = 25.4 bits (54), Expect = 9.3
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = +1
Query: 121 DSDDDEDGSEMSHDDFVD 138
D DDD+D S++ DD++D
Sbjct: 601 DDDDDDDLSDLFMDDYMD 654
>TC76935 similar to SP|P21747|EA92_VICFA Embryonic abundant protein USP92
precursor. [Broad bean] {Vicia faba}, partial (40%)
Length = 1840
Score = 25.4 bits (54), Expect = 9.3
Identities = 30/106 (28%), Positives = 48/106 (44%), Gaps = 16/106 (15%)
Frame = -1
Query: 3 MYVLCFRMRSLVDIPRLKLQLLNMPMEPIWK-----HKLSPLK---VCLPTVVEEFLRQA 54
MYV F+ L+ L+L+LL+ M+ I K H + + +C ++ R
Sbjct: 997 MYV--FQKVHLLFQSLLQLRLLSRSMKKIQKVDLFYHSMQVFQNVGLCFSCPLQFLSRSK 824
Query: 55 KAAQ---LFMSSSETF-----VFNDLLESDDLSRSFGGIDRLDMFF 92
+ Q LF S F +F LL+ LSRS I ++D+F+
Sbjct: 823 RKTQKVDLFYHSMYVFQKVHLLFQSLLQIRLLSRSMKKIQKVDLFY 686
>TC87624 similar to GP|12321915|gb|AAG50994.1 unknown protein; 32328-30725
{Arabidopsis thaliana}, partial (41%)
Length = 1159
Score = 25.4 bits (54), Expect = 9.3
Identities = 8/24 (33%), Positives = 16/24 (66%)
Frame = -3
Query: 9 RMRSLVDIPRLKLQLLNMPMEPIW 32
R++SL+D P++ +P++ IW
Sbjct: 815 RLKSLIDAPQVNFSWFIIPVQKIW 744
>AL389286 similar to GP|17862852|gb| RE01016p {Drosophila melanogaster},
partial (4%)
Length = 488
Score = 25.4 bits (54), Expect = 9.3
Identities = 11/28 (39%), Positives = 17/28 (60%), Gaps = 5/28 (17%)
Frame = +3
Query: 23 LLNMPMEPIW-----KHKLSPLKVCLPT 45
L+N+P +W K K+SP K+ +PT
Sbjct: 174 LINLPNMEVWNSVVEKAKISPFKIQIPT 257
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.139 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,682,288
Number of Sequences: 36976
Number of extensions: 59915
Number of successful extensions: 524
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 509
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 519
length of query: 153
length of database: 9,014,727
effective HSP length: 88
effective length of query: 65
effective length of database: 5,760,839
effective search space: 374454535
effective search space used: 374454535
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0252a.4