BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0250.7
(423 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF518434 similar to GP|12055493|em geranyl diphosphat synthase {... 278 2e-75
TC84959 similar to GP|19911233|dbj|BAB86941. solanesyl diphospha... 135 4e-32
TC87480 similar to GP|6277254|dbj|BAA86284.1 geranylgeranyl pyro... 79 3e-15
AJ502290 similar to PIR|T11021|T11 farnesyltranstransferase (EC ... 51 1e-06
AW208031 weakly similar to GP|12055493|emb geranyl diphosphat sy... 43 3e-04
TC89587 similar to PIR|C86306|C86306 prenyl transferase homolog ... 43 3e-04
TC85749 homologue to SP|P49351|FPP1_LUPAL Farnesyl pyrophosphate... 40 0.002
TC91745 similar to GP|11692820|gb|AAG40013.1 AT4g38460 {Arabidop... 35 0.042
TC78624 similar to GP|4850385|gb|AAD31055.1| EST gb|T22808 comes... 30 2.3
TC78229 similar to GP|4163997|gb|AAD05539.1| alpha-xylosidase pr... 30 2.3
TC90193 homologue to PIR|F85355|F85355 hypothetical protein AT4g... 29 3.9
TC84096 similar to GP|21741983|emb|CAD41033. OSJNBa0060P14.4 {Or... 28 8.7
TC83395 weakly similar to GP|19387240|gb|AAL87152.1 putative exo... 28 8.7
>BF518434 similar to GP|12055493|em geranyl diphosphat synthase {Quercus
robur}, partial (33%)
Length = 512
Score = 278 bits (712), Expect = 2e-75
Identities = 146/170 (85%), Positives = 154/170 (89%), Gaps = 1/170 (0%)
Frame = +3
Query: 58 KGLPALHSSRYQIHDQ-SSSISEEELDPFSLVADELSFIANNLRAMVIAEVPKLASGAEY 116
KG+PAL+SSRY IH SSS+ E+ELDPFSLVADELSFI N LR MV+A+VPKLAS AEY
Sbjct: 3 KGMPALYSSRYHIHHHTSSSLVEDELDPFSLVADELSFIGNKLREMVVAKVPKLASAAEY 182
Query: 117 FFKMGVEGKRFRPTVLLLMSTALNLPIPKPPPSNDLGGTFTADLRSRQQRIAEITEMIHV 176
FFKMGVEGKRFRPTVLL+MSTA NLPIPK PP +LGGT T DLRSRQQRIAEITEMIHV
Sbjct: 183 FFKMGVEGKRFRPTVLLIMSTA*NLPIPKAPPPMELGGTTTTDLRSRQQRIAEITEMIHV 362
Query: 177 ASLLHDDVLDDADTRRGIGSLNFVMGNKLAVLAGDFLLSRACVALASLKN 226
ASLLHDDVLDDADTRRGIGSLNFVMGNKLAVLAGDFLLSRA VALASLKN
Sbjct: 363 ASLLHDDVLDDADTRRGIGSLNFVMGNKLAVLAGDFLLSRASVALASLKN 512
>TC84959 similar to GP|19911233|dbj|BAB86941. solanesyl diphosphate synthase
{Arabidopsis thaliana}, partial (46%)
Length = 826
Score = 135 bits (339), Expect = 4e-32
Identities = 67/171 (39%), Positives = 112/171 (65%)
Frame = +1
Query: 229 VVSLLAKVVEHLVTGETMQMSTTSDQRCSMEYYMQKTYYKTASLISNSCKAIAILAGQTA 288
V+ L+++V++ +GE Q S+ D ++ Y+ K+Y+KTASLI+ S K A+ +G +
Sbjct: 1 VIKLISQVIKDFASGEIKQASSLFDCDVQLDEYLIKSYFKTASLIAASVKGAAVFSGSDS 180
Query: 289 DVAMLAFDYGKNLGLAFQLIDDVLDFTGTSASLGKGSLSDIRHGIVTAPILFAMEEFPQL 348
+ F+YGKNLGL+FQ++DD+LDFT ++ LGK + +D+ G +TAP++FA+E+ P+L
Sbjct: 181 SMTEKMFEYGKNLGLSFQVVDDILDFTQSAEQLGKPAGTDLAKGNLTAPVIFALEKEPKL 360
Query: 349 RTVVDEGFDNPENVDIALEYLGKSRGIQRTRELAIKHADLAAAAIDSLPES 399
R +++ F +D A++ + GI+R +ELA + ADLA + LP S
Sbjct: 361 RDIIEFEFSEIGFLDEAIKLVKSCGGIERAQELAKEKADLAIQSFQCLPPS 513
>TC87480 similar to GP|6277254|dbj|BAA86284.1 geranylgeranyl pyrophosphate
synthase {Croton sublyratus}, partial (79%)
Length = 1583
Score = 79.0 bits (193), Expect = 3e-15
Identities = 70/240 (29%), Positives = 109/240 (45%), Gaps = 11/240 (4%)
Frame = +1
Query: 101 AMVIAEVPKLASGAEYFFKMGVEGKRFRPTVLLLMSTALNLPIPKPPPSNDLGGTFTADL 160
A+ + E K+ Y G GKR RP + L + P P+
Sbjct: 580 AVSLREPLKVHEAMRYSLLAG--GKRVRPVLCLAACELVGGTEPMAMPA----------- 720
Query: 161 RSRQQRIAEITEMIHVASLLHDDV--LDDADTRRGIGSLNFVMGNKLAVLAGDFLLSRA- 217
A EMIH SL+HDD+ +D+ D RRG + + V G +AVLAGD LL+ A
Sbjct: 721 -------ACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDVAVLAGDALLAFAF 879
Query: 218 ------CVALASLKNTEVVSLLAKVV--EHLVTGETMQMSTTSDQRCSMEYYMQKTYYKT 269
V ++ + + LAK + E LV G+ + +++ +E +KT
Sbjct: 880 EHIAVSTVDVSPARIVRAIGELAKSIGSEGLVAGQVVDINSEGLSDVGLERLEFIHLHKT 1059
Query: 270 ASLISNSCKAIAILAGQTADVAMLAFDYGKNLGLAFQLIDDVLDFTGTSASLGKGSLSDI 329
A+L+ + AIL G + + + +GL FQ++DD+LD T +S LGK + D+
Sbjct: 1060AALLEGAVVLGAILGGGSDEDVEKLRKFATYIGLLFQVVDDILDVTKSSQELGKTAGKDL 1239
>AJ502290 similar to PIR|T11021|T11 farnesyltranstransferase (EC 2.5.1.29) -
white lupine, partial (41%)
Length = 657
Score = 50.8 bits (120), Expect = 1e-06
Identities = 45/119 (37%), Positives = 59/119 (48%), Gaps = 2/119 (1%)
Frame = +2
Query: 101 AMVIAEVPKLASGAEYFFKMGVEGKRFRPTVLLLMSTALNLPIPKPPPSNDLGGTFTADL 160
A+V+ E K+ Y G GKR RP VL + + L +GG A +
Sbjct: 320 AIVLQEPEKIYEAMRYSLLAG--GKRVRP-VLCIAACGL------------VGGKEEASI 454
Query: 161 RSRQQRIAEITEMIHVASLLHDDV--LDDADTRRGIGSLNFVMGNKLAVLAGDFLLSRA 217
S A EMIH SL+HDD+ +D+ D RRG + + V G +AVLAGD LLS A
Sbjct: 455 PS-----ACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVYGEDVAVLAGDALLSFA 616
>AW208031 weakly similar to GP|12055493|emb geranyl diphosphat synthase
{Quercus robur}, partial (5%)
Length = 375
Score = 42.7 bits (99), Expect = 3e-04
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = +2
Query: 8 SRISRNLRGSLNGTRRLLSIQEQNHQSLQSHHYHTPTGST 47
SRISRNLRG+ NG RR LSI E N + L S H+P ST
Sbjct: 260 SRISRNLRGAFNGYRRFLSIGENNRRFLFS---HSPADST 370
>TC89587 similar to PIR|C86306|C86306 prenyl transferase homolog [imported]
- Arabidopsis thaliana, partial (27%)
Length = 714
Score = 42.7 bits (99), Expect = 3e-04
Identities = 19/34 (55%), Positives = 25/34 (72%)
Frame = +1
Query: 159 DLRSRQQRIAEITEMIHVASLLHDDVLDDADTRR 192
DL + +R+A I EMIH ASL+HDDVL ++D R
Sbjct: 598 DLTVKHRRLAXIVEMIHTASLIHDDVLXESDLXR 699
>TC85749 homologue to SP|P49351|FPP1_LUPAL Farnesyl pyrophosphate synthetase
1 (FPP synthetase 1) (FPS 1) (Farnesyl diphosphate
synthetase 1), complete
Length = 1403
Score = 39.7 bits (91), Expect = 0.002
Identities = 48/210 (22%), Positives = 90/210 (42%), Gaps = 15/210 (7%)
Frame = +2
Query: 172 EMIHVASLLHDDVLDDADTRRGIGSLNFVMGNKLAVLAGDFLLSRACVALASLKN----- 226
E + L+ DD++D++ TRRG + + + A D +L R + K+
Sbjct: 305 EWLQAYFLVLDDIMDNSHTRRG-QPCWYRVPKVGMIAANDGVLLRNHIPRILRKHFKGKP 481
Query: 227 --TEVVSLLAKVVEHLVTGETMQMSTTSD-----QRCSMEYYMQKTYYKTASLISNSCKA 279
E++ L +V G+ + + TT + + ++ + + YKTA A
Sbjct: 482 YYVELLDLFNEVEFQTAAGQMIDLITTLEGEKDLSKYTLSLHRRIVQYKTAYYSFYLPVA 661
Query: 280 IAIL-AGQTADVAMLAFDYGKNLGLAFQLIDDVLDFTGTSASLGK--GSLSDIRHGIVTA 336
A+L AG+ D + + +G FQ+ DD LD G ++GK + D + V
Sbjct: 662 CALLMAGENLDNHVDVKNILVEMGTYFQVQDDYLDCFGDPETIGKIGTDIEDFKCSWVVV 841
Query: 337 PILFAMEEFPQLRTVVDEGFDNPENVDIAL 366
L E + + V+ E + P+ ++A+
Sbjct: 842 KALELCNE--EQKKVLHENYGKPDPANVAI 925
>TC91745 similar to GP|11692820|gb|AAG40013.1 AT4g38460 {Arabidopsis
thaliana}, partial (33%)
Length = 539
Score = 35.4 bits (80), Expect = 0.042
Identities = 18/41 (43%), Positives = 28/41 (67%), Gaps = 2/41 (4%)
Frame = +2
Query: 172 EMIHVASLLHDDV--LDDADTRRGIGSLNFVMGNKLAVLAG 210
EM+H ASL+HDD+ +DD+ +RRG S + + G +A+ G
Sbjct: 413 EMVHAASLIHDDLPCMDDSLSRRGQPSNHTIYGVDMAISWG 535
>TC78624 similar to GP|4850385|gb|AAD31055.1| EST gb|T22808 comes from this
gene. {Arabidopsis thaliana}, partial (51%)
Length = 2052
Score = 29.6 bits (65), Expect = 2.3
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = -2
Query: 124 GKRFRPTVLLLMSTALNLPIPKPPP 148
G+ F PT L +AL+ P PKPPP
Sbjct: 788 GRGFLPTAQLSTPSALDSPPPKPPP 714
>TC78229 similar to GP|4163997|gb|AAD05539.1| alpha-xylosidase precursor
{Arabidopsis thaliana}, partial (25%)
Length = 921
Score = 29.6 bits (65), Expect = 2.3
Identities = 22/73 (30%), Positives = 34/73 (46%)
Frame = +2
Query: 14 LRGSLNGTRRLLSIQEQNHQSLQSHHYHTPTGSTAKVMRSLAFSKGLPALHSSRYQIHDQ 73
++ S +G QE NH HHYH P S+ K + + S + +S+ +QI
Sbjct: 308 MQTSKDGKYHTTLSQENNH-----HHYHKP*ESSKKTLLK-SQSIQAQSFYSATFQIPLV 469
Query: 74 SSSISEEELDPFS 86
S S + + PFS
Sbjct: 470 SQSKENQMVKPFS 508
>TC90193 homologue to PIR|F85355|F85355 hypothetical protein AT4g30410
[imported] - Arabidopsis thaliana, partial (8%)
Length = 751
Score = 28.9 bits (63), Expect = 3.9
Identities = 16/44 (36%), Positives = 22/44 (49%)
Frame = -2
Query: 180 LHDDVLDDADTRRGIGSLNFVMGNKLAVLAGDFLLSRACVALAS 223
L D + + + S+NF GNKL L FL +R + LAS
Sbjct: 594 LRDSI*SRVSSTKHASSMNFPPGNKLLSLLVLFLTNRVAIKLAS 463
>TC84096 similar to GP|21741983|emb|CAD41033. OSJNBa0060P14.4 {Oryza
sativa}, partial (11%)
Length = 556
Score = 27.7 bits (60), Expect = 8.7
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -1
Query: 122 VEGKRFRPTVLLLMSTALNLPIPKPPPSNDLGGTFTADL 160
VEG + P VLL+++ + LP P P N L G F L
Sbjct: 187 VEG--YAPGVLLVITVGVGLPSLCPEPENGLEGCFGGGL 77
>TC83395 weakly similar to GP|19387240|gb|AAL87152.1 putative exonuclease
{Oryza sativa (japonica cultivar-group)}, partial (67%)
Length = 941
Score = 27.7 bits (60), Expect = 8.7
Identities = 18/72 (25%), Positives = 31/72 (43%), Gaps = 12/72 (16%)
Frame = -1
Query: 13 NLRGSLNGTRRLLSIQEQ------------NHQSLQSHHYHTPTGSTAKVMRSLAFSKGL 60
+L S++GT + LS +Q NH ++ +H PT ++ + F+K
Sbjct: 863 SLSSSMDGTLQCLSSSDQDNSFPSNPNHVPNHALQEAWPFHHPT*LFQWILHCIKFAKSF 684
Query: 61 PALHSSRYQIHD 72
P L Y + D
Sbjct: 683 PELP*VAYHLED 648
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.134 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,678,127
Number of Sequences: 36976
Number of extensions: 139722
Number of successful extensions: 1000
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 987
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 995
length of query: 423
length of database: 9,014,727
effective HSP length: 99
effective length of query: 324
effective length of database: 5,354,103
effective search space: 1734729372
effective search space used: 1734729372
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0250.7