BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0234.13
(1451 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG587143 weakly similar to PIR|A96586|A9 hypothetical protein F2... 206 5e-53
BE941280 weakly similar to GP|20197614|g unknown protein {Arabid... 147 2e-35
BG586306 weakly similar to GP|15128241|db helicase-like protein ... 137 3e-32
BM814840 weakly similar to GP|15128241|db helicase-like protein ... 133 4e-31
TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein... 96 1e-19
BI271341 87 5e-17
CB892155 similar to PIR|D86481|D86 hypothetical protein AAG28292... 77 4e-14
CA859513 58 3e-08
CB892166 similar to GP|20197614|gb unknown protein {Arabidopsis ... 46 1e-04
TC84660 similar to PIR|D86481|D86481 hypothetical protein AAG282... 39 0.015
TC80146 37 0.073
AJ497367 similar to GP|14140286|gb putative helicase {Oryza sati... 34 0.36
AW685809 similar to PIR|E84792|E8 probable kinesin heavy chain [... 32 1.4
CB894488 similar to GP|6437560|gb| hypothetical protein {Arabido... 31 4.0
TC81851 weakly similar to PIR|T47750|T47750 N2 N2-dimethylguanos... 30 5.2
TC93066 weakly similar to GP|19920130|gb|AAM08562.1 Putative ret... 30 5.2
TC92104 similar to PIR|D86151|D86151 hypothetical protein AAF764... 30 6.8
CB893618 weakly similar to GP|3947733|emb| NL25 {Solanum tuberos... 30 6.8
>BG587143 weakly similar to PIR|A96586|A9 hypothetical protein F20D21.24
[imported] - Arabidopsis thaliana, partial (12%)
Length = 717
Score = 206 bits (524), Expect = 5e-53
Identities = 94/196 (47%), Positives = 133/196 (66%), Gaps = 1/196 (0%)
Frame = -3
Query: 462 DRPDISCRVFHIKVKQLMRDLRKGQYFGKVSAGMYTIEFQKRGLPHAHILIWLAPGSKLT 521
D+PDI CRVF +K+ +L++D +KG +F + ++ IEFQKRGL HAHIL+W S+
Sbjct: 688 DKPDIECRVFKMKLDELLKDFKKGTFFKPYTTALHRIEFQKRGLRHAHILLWFGNSSRTP 509
Query: 522 TPEKIDSVICAELPDPVASPKLFEVVSMFMVHGPCGSSRKNSPCMVNGRCSKFFPKKYVD 581
+ E++D +I AELP+ P+ + +V+ M+HGPCG SPCM N C+K +P+ Y
Sbjct: 508 SSEEVDEIISAELPNKKQDPEAYNLVTKHMIHGPCGVINPKSPCMENNVCTKKYPRPYNG 329
Query: 582 KTSFDIDGYPTYRRR-NTGVFVERRDVQLDNGYVVPYNAKLLMKYQAHINIVYCNKSNCI 640
TS D GY YRRR N V + L+N ++VP+N KLL KY+AHIN+ +CN+++ +
Sbjct: 328 STSIDKSGYVLYRRR*NETEHVVKNGAILNNTFIVPHNIKLLKKYEAHINMEWCNRTSAV 149
Query: 641 KYLFKYINKGVDRVTV 656
KYLFKYI KGVDRV+V
Sbjct: 148 KYLFKYITKGVDRVSV 101
>BE941280 weakly similar to GP|20197614|g unknown protein {Arabidopsis
thaliana}, partial (8%)
Length = 403
Score = 147 bits (372), Expect = 2e-35
Identities = 74/134 (55%), Positives = 95/134 (70%)
Frame = -1
Query: 996 VYGSGGTGKTFLWKTLSYRLRSERKIVLNVASSGITSLLLPGGRTAHSLFSIPLVLNEDS 1055
+Y GGT KTF+W+ LS LRSE +IVL ASSGI +LL+PGGRTAHS F IP +++E S
Sbjct: 403 LYDYGGTEKTFIWRALSAALRSEGEIVLACASSGIDALLMPGGRTAHSRFGIPFIIDETS 224
Query: 1056 CCNIRLGSNKAELLKHTSLIIWDEAPMVNRWAFEAVDRTLRDIMEVGDVYGGGKPFGGKV 1115
C + A L+ LIIWDEAPM+++ FEA+DR+LRD+++ D PFGGKV
Sbjct: 223 MCGVTPNIPLASLVIKAKLIIWDEAPMMHKHCFEALDRSLRDVLKTVDERNKDIPFGGKV 44
Query: 1116 VVLGGDFRQTLPII 1129
VVLGGDFRQ L ++
Sbjct: 43 VVLGGDFRQILLVM 2
>BG586306 weakly similar to GP|15128241|db helicase-like protein {Oryza
sativa (japonica cultivar-group)}, partial (2%)
Length = 667
Score = 137 bits (345), Expect = 3e-32
Identities = 86/229 (37%), Positives = 127/229 (54%)
Frame = +2
Query: 80 REFLANIRSYNSAFAFTSFGGKIESGLNDGGGPPQFVISGQNYHRIGSLLPNVGETPKFA 139
R F IR YNS AFTS G K++ + + G I GQ +HRI SL+P G P++
Sbjct: 5 RWFRDTIRVYNSVLAFTSIGMKMDYSVVNAPGRYTIRIQGQTHHRIDSLIPRQGRPPEYL 184
Query: 140 QLYVYDTQNEIQNRSSHFRYVNAIFSYECCILCFNVINIFVLLFRNSDGSCKLNKSLIED 199
Q+Y++DT NE++NR +NA+ +++G+ L+++ +
Sbjct: 185 QIYIFDTGNEVRNR------LNAMGQ------------------TSTEGN--LDETTLAR 286
Query: 200 LLAMVDECNVLVKSFRKVRDFISINPLLRISLRLFRARPKDPRVYNLPSVDEVAGLIVGD 259
L+ M+DE N L K FR+ RD+ + ++RL + K + Y+LPS EVAGLIVGD
Sbjct: 287 LIEMIDENNCLAKLFRRARDYYKGSGQ-EFNIRLLSDKGKG-KEYDLPSTSEVAGLIVGD 460
Query: 260 FDSTDCGRDIVVSSMDGTLRRIHETHTSFLPLQYPFLFPNGEDGYKEDI 308
ST RDIVV TL++I + H+ ++ LQYP LFP GE G+ +I
Sbjct: 461 MSSTIGVRDIVVQFQSDTLQQIRDYHSLYMSLQYPLLFPYGEYGFHPEI 607
>BM814840 weakly similar to GP|15128241|db helicase-like protein {Oryza sativa
(japonica cultivar-group)}, partial (6%)
Length = 733
Score = 133 bits (335), Expect = 4e-31
Identities = 65/127 (51%), Positives = 92/127 (72%), Gaps = 1/127 (0%)
Frame = +3
Query: 1326 NGTRLIVLDLRPNLIYGKVLNGNKAGTKTYIPRMTIVPSDSGLHVKVQRRQFPVCVCFAM 1385
+GTRLI++ L N+I +V+ G AG +YIPRM ++PS + + + +R QFP+ + FAM
Sbjct: 27 HGTRLIIVSLGKNVICARVIGGTHAGEVSYIPRMNLIPSGANVSITFERCQFPLVLSFAM 206
Query: 1386 TINKSQGQTLSRVGVFLPRPVFSHGQLYVALSRVKSRDGLKIYV-DQTEVPPLEHTKMLF 1444
TINKSQGQTL+ VG++LPRPVF+HGQLYVA+SRVKSR GLKI + D+ P +++
Sbjct: 207 TINKSQGQTLTSVGLYLPRPVFTHGQLYVAVSRVKSRSGLKILITDENGSPSSSTVNVVY 386
Query: 1445 TKKYSKI 1451
+ + KI
Sbjct: 387 QEVFQKI 407
>TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein T24M8.10 -
Arabidopsis thaliana, partial (11%)
Length = 701
Score = 95.5 bits (236), Expect = 1e-19
Identities = 55/143 (38%), Positives = 88/143 (61%)
Frame = +3
Query: 1175 VEFSKWILDIGDGNLGDYNDGECDLDIPHDLMVPFKDDAVSSIVYSTYPDIQRKFFEEEY 1234
VEF K +I G + + ND ++D P D + +IV STYP++ ++ E++
Sbjct: 303 VEF-KTFAEILTGKMSEPNDSYAEVDTP-------PGDPIDAIVQSTYPNLVSQYNNEQF 458
Query: 1235 FIDKAILAPTLDIVDSVNQFVLSIVPGKEKVYLSSDSVVKVDEDVAIDANWITIEFLNGI 1294
+AIL T ++VD +N +VL ++PG+E+V S++ +V++ A DA I EFL +
Sbjct: 459 LQSRAILTSTDEVVDQINDYVLKLIPGEERVIYSANR-SEVNDVQAFDA--IPPEFLQSL 629
Query: 1295 KGSGLPDHRLCLKIGTPIMLMRN 1317
K S LP+H+L LK+GTPIML+R+
Sbjct: 630 KTSDLPNHKLTLKVGTPIMLLRD 698
>BI271341
Length = 468
Score = 87.0 bits (214), Expect = 5e-17
Identities = 44/101 (43%), Positives = 66/101 (64%)
Frame = +1
Query: 1122 FRQTLPIIPKASREEIVMATINSSRLWKFCKVLKLTENMCLHGNDSLHDCEKLVEFSKWI 1181
FR LP+IP+ SR +I+ ATINSS + C+V++L +NM L N + + +FSK I
Sbjct: 1 FR*ILPVIPRGSRSDIIHATINSSCI*DHCQVVRLKKNMWLQQNGQSSNDPEFEQFSK*I 180
Query: 1182 LDIGDGNLGDYNDGECDLDIPHDLMVPFKDDAVSSIVYSTY 1222
L +GDG + + ND D+DIP +L++ DD++ +IV STY
Sbjct: 181 LKVGDGKIYEPNDSYADIDIPPELLISNYDDSLQTIVQSTY 303
>CB892155 similar to PIR|D86481|D86 hypothetical protein AAG28292.1 [imported]
- Arabidopsis thaliana, partial (4%)
Length = 572
Score = 77.4 bits (189), Expect = 4e-14
Identities = 43/83 (51%), Positives = 49/83 (58%)
Frame = -2
Query: 1351 GTKTYIPRMTIVPSDSGLHVKVQRRQFPVCVCFAMTINKSQGQTLSRVGVFLPRPVFSHG 1410
G RM D V V V FAMTINKSQGQ+L +GV+LP VFSHG
Sbjct: 454 GRSVQYKRMKGADIDMSQGVSQMSTHESVEVYFAMTINKSQGQSLKHIGVYLPSSVFSHG 275
Query: 1411 QLYVALSRVKSRDGLKIYVDQTE 1433
QLYVALSRV SR+GLKI + +
Sbjct: 274 QLYVALSRVTSREGLKILISNDD 206
>CA859513
Length = 363
Score = 57.8 bits (138), Expect = 3e-08
Identities = 29/72 (40%), Positives = 39/72 (53%)
Frame = +1
Query: 495 MYTIEFQKRGLPHAHILIWLAPGSKLTTPEKIDSVICAELPDPVASPKLFEVVSMFMVHG 554
+Y EF+K LPHAH+L A + D+ + AEL DPV +L++ V M+HG
Sbjct: 136 IYVAEFEKCDLPHAHMLFHRA--------DNYDTFVSAELSDPVEQLRLYQTVVSVMIHG 291
Query: 555 PCGSSRKNSPCM 566
P G N PCM
Sbjct: 292 PYGPFNNNVPCM 327
>CB892166 similar to GP|20197614|gb unknown protein {Arabidopsis thaliana},
partial (3%)
Length = 748
Score = 45.8 bits (107), Expect = 1e-04
Identities = 23/51 (45%), Positives = 32/51 (62%)
Frame = -1
Query: 1401 FLPRPVFSHGQLYVALSRVKSRDGLKIYVDQTEVPPLEHTKMLFTKKYSKI 1451
F R VFSHGQLYVA+SRV SR+GLKI + +++T + K + +
Sbjct: 289 FYDREVFSHGQLYVAISRVSSRNGLKILMIDENGDCIDNTTNVVYKVFQNV 137
>TC84660 similar to PIR|D86481|D86481 hypothetical protein AAG28292.1
[imported] - Arabidopsis thaliana, partial (1%)
Length = 1009
Score = 38.9 bits (89), Expect = 0.015
Identities = 17/29 (58%), Positives = 24/29 (82%)
Frame = +2
Query: 1394 TLSRVGVFLPRPVFSHGQLYVALSRVKSR 1422
+++ G++LP+P+F HG LYVALSRV SR
Sbjct: 68 SMATKGMYLPQPIF*HG*LYVALSRVTSR 154
>TC80146
Length = 476
Score = 36.6 bits (83), Expect = 0.073
Identities = 15/28 (53%), Positives = 23/28 (81%)
Frame = -3
Query: 1386 TINKSQGQTLSRVGVFLPRPVFSHGQLY 1413
TINKS+ Q+LS + ++L RP+FSH ++Y
Sbjct: 450 TINKSR*QSLSYMKIYLSRPIFSHEEMY 367
>AJ497367 similar to GP|14140286|gb putative helicase {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 543
Score = 34.3 bits (77), Expect = 0.36
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +1
Query: 1407 FSHGQLYVALSRVKSRDGLKI 1427
FS+G+LYVA+SRV SR GLKI
Sbjct: 19 FSNGKLYVAVSRVTSRKGLKI 81
>AW685809 similar to PIR|E84792|E8 probable kinesin heavy chain [imported] -
Arabidopsis thaliana, partial (14%)
Length = 651
Score = 32.3 bits (72), Expect = 1.4
Identities = 28/86 (32%), Positives = 40/86 (45%), Gaps = 15/86 (17%)
Frame = +2
Query: 936 PISDEVPQFE--NVMLFNELRFDIDDMSVKHNDHLMKLNN---------GQRKVYDEVI- 983
P+SDE + V+ NE + ++ M N + K+ N QR +YD+ I
Sbjct: 278 PLSDEEQRSNVPKVVSCNENKREVTVMHTIANKQVEKVFNFDKVFGPKSQQRSIYDQAIA 457
Query: 984 DAVNKSDGGF---YFVYGSGGTGKTF 1006
VN+ GF F YG GTGKT+
Sbjct: 458 PIVNEVLDGFNCTVFAYGQTGTGKTY 535
>CB894488 similar to GP|6437560|gb| hypothetical protein {Arabidopsis
thaliana}, partial (37%)
Length = 830
Score = 30.8 bits (68), Expect = 4.0
Identities = 18/55 (32%), Positives = 25/55 (44%)
Frame = +1
Query: 1356 IPRMTIVPSDSGLHVKVQRRQFPVCVCFAMTINKSQGQTLSRVGVFLPRPVFSHG 1410
IP + VP S LHV V +P T+ G + ++GV + RP HG
Sbjct: 643 IPAPSPVPPVSSLHVPVPSPLYP-------TVQTPSGPSSQQIGVLVARPPLLHG 786
>TC81851 weakly similar to PIR|T47750|T47750 N2 N2-dimethylguanosine tRNA
methyltransferases-like protein - Arabidopsis thaliana,
partial (56%)
Length = 1046
Score = 30.4 bits (67), Expect = 5.2
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = +3
Query: 157 FRYVNAIFSYECCILCFNVINIFVLLFRNS 186
F Y ++F++ CC LC +++F+L+ R S
Sbjct: 33 FSYFFSVFAFRCCCLCLG-LSLFLLILRKS 119
>TC93066 weakly similar to GP|19920130|gb|AAM08562.1 Putative retroelement
{Oryza sativa} [Oryza sativa (japonica cultivar-group)],
partial (10%)
Length = 823
Score = 30.4 bits (67), Expect = 5.2
Identities = 19/53 (35%), Positives = 31/53 (57%), Gaps = 1/53 (1%)
Frame = -2
Query: 527 DSVICAELPDPVASPKLFEVVSMFMVHG-PCGSSRKNSPCMVNGRCSKFFPKK 578
+++ICAEL V + +L ++ ++H PC S KNSP + G+ F P+K
Sbjct: 363 NTMICAELIK-VTTTEL*SIICYELLHILPCLSFNKNSPLLECGKSLIFIPQK 208
>TC92104 similar to PIR|D86151|D86151 hypothetical protein AAF76473.1
[imported] - Arabidopsis thaliana, partial (10%)
Length = 462
Score = 30.0 bits (66), Expect = 6.8
Identities = 21/59 (35%), Positives = 30/59 (50%), Gaps = 4/59 (6%)
Frame = +2
Query: 952 ELRFDIDDMSVKHNDHLMKLNNGQRKVYDEVIDAVNKS--DG--GFYFVYGSGGTGKTF 1006
+LR + D D ++ + Q++VY+ V V +S DG G YG GTGKTF
Sbjct: 266 KLRRNNWDSDTYEFDEVLTQSASQKRVYEVVAKPVVESVLDGYNGTVMAYGQTGTGKTF 442
>CB893618 weakly similar to GP|3947733|emb| NL25 {Solanum tuberosum}, partial
(18%)
Length = 927
Score = 30.0 bits (66), Expect = 6.8
Identities = 17/40 (42%), Positives = 23/40 (57%), Gaps = 5/40 (12%)
Frame = -1
Query: 436 LFITVTCNPKWLEIQRCVSEK--GLNAYDRPDI---SCRV 470
L+ TCNPK+L +Q C GL+ +P+I SCRV
Sbjct: 498 LYDLETCNPKYLYVQLCFQAP*CGLSRRSQPEILPTSCRV 379
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.141 0.433
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,721,145
Number of Sequences: 36976
Number of extensions: 724182
Number of successful extensions: 3468
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 3351
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3463
length of query: 1451
length of database: 9,014,727
effective HSP length: 108
effective length of query: 1343
effective length of database: 5,021,319
effective search space: 6743631417
effective search space used: 6743631417
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0234.13