BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0233.16
(1520 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM814840 weakly similar to GP|15128241|db helicase-like protein ... 166 6e-41
BE941280 weakly similar to GP|20197614|g unknown protein {Arabid... 160 3e-39
CB892155 similar to PIR|D86481|D86 hypothetical protein AAG28292... 100 6e-21
TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein... 80 6e-15
BI271341 77 7e-14
BG587143 weakly similar to PIR|A96586|A9 hypothetical protein F2... 70 5e-12
CB892166 similar to GP|20197614|gb unknown protein {Arabidopsis ... 57 4e-08
AJ497367 similar to GP|14140286|gb putative helicase {Oryza sati... 57 5e-08
CA859513 44 5e-04
TC84660 similar to PIR|D86481|D86481 hypothetical protein AAG282... 41 0.004
TC80146 39 0.020
BE998307 34 0.38
BQ137420 30 5.5
TC83061 similar to GP|15292835|gb|AAK92786.1 unknown protein {Ar... 30 5.5
TC90214 similar to SP|P55748|CP22_HORVU Serine carboxypeptidase ... 30 7.1
TC84042 similar to PIR|T48214|T48214 endo-1 4-beta-mannosidase-l... 30 7.1
AW688169 weakly similar to GP|21745054|gb| putative disease resi... 30 9.3
>BM814840 weakly similar to GP|15128241|db helicase-like protein {Oryza sativa
(japonica cultivar-group)}, partial (6%)
Length = 733
Score = 166 bits (420), Expect = 6e-41
Identities = 83/127 (65%), Positives = 104/127 (81%)
Frame = +3
Query: 1393 NGTRLIVDYLGPNVIGAIVLSGTHIGKVVYISRMNLMPSDESMPVKFQRRQFPLIASFAM 1452
+GTRLI+ LG NVI A V+ GTH G+V YI RMNL+PS ++ + F+R QFPL+ SFAM
Sbjct: 27 HGTRLIIVSLGKNVICARVIGGTHAGEVSYIPRMNLIPSGANVSITFERCQFPLVLSFAM 206
Query: 1453 TINKSQGQSLSQVGVYLPKPVFSHGQLYVAISRVKSRAGLKILICNEDTSQLDVTKNIVF 1512
TINKSQGQ+L+ VG+YLP+PVF+HGQLYVA+SRVKSR+GLKILI +E+ S T N+V+
Sbjct: 207 TINKSQGQTLTSVGLYLPRPVFTHGQLYVAVSRVKSRSGLKILITDENGSPSSSTVNVVY 386
Query: 1513 QEVFQKI 1519
QEVFQKI
Sbjct: 387 QEVFQKI 407
>BE941280 weakly similar to GP|20197614|g unknown protein {Arabidopsis
thaliana}, partial (8%)
Length = 403
Score = 160 bits (406), Expect = 3e-39
Identities = 78/134 (58%), Positives = 100/134 (74%)
Frame = -1
Query: 1063 VYGYGGTGKTFLWKTLTYKLRSQKQIILNVASSGIASLLLPGGRTAHSLFSIPLCLNEDS 1122
+Y YGGT KTF+W+ L+ LRS+ +I+L ASSGI +LL+PGGRTAHS F IP ++E S
Sbjct: 403 LYDYGGTEKTFIWRALSAALRSEGEIVLACASSGIDALLMPGGRTAHSRFGIPFIIDETS 224
Query: 1123 CCGIPQGSPKAKLLQLASLIIWDEAPMVSRYAFEALDRTLRDIMRFKIHNSSDKPFGGKV 1182
CG+ P A L+ A LIIWDEAPM+ ++ FEALDR+LRD+++ + D PFGGKV
Sbjct: 223 MCGVTPNIPLASLVIKAKLIIWDEAPMMHKHCFEALDRSLRDVLKTVDERNKDIPFGGKV 44
Query: 1183 VVLGGDFRQILPVI 1196
VVLGGDFRQIL V+
Sbjct: 43 VVLGGDFRQILLVM 2
>CB892155 similar to PIR|D86481|D86 hypothetical protein AAG28292.1 [imported]
- Arabidopsis thaliana, partial (4%)
Length = 572
Score = 100 bits (248), Expect = 6e-21
Identities = 55/102 (53%), Positives = 64/102 (61%)
Frame = -2
Query: 1418 GKVVYISRMNLMPSDESMPVKFQRRQFPLIASFAMTINKSQGQSLSQVGVYLPKPVFSHG 1477
G+ V RM D S V + FAMTINKSQGQSL +GVYLP VFSHG
Sbjct: 454 GRSVQYKRMKGADIDMSQGVSQMSTHESVEVYFAMTINKSQGQSLKHIGVYLPSSVFSHG 275
Query: 1478 QLYVAISRVKSRAGLKILICNEDTSQLDVTKNIVFQEVFQKI 1519
QLYVA+SRV SR GLKILI N+D VT N+V++EVF +
Sbjct: 274 QLYVALSRVTSREGLKILISNDDGEDDCVTSNVVYREVFHNL 149
>TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein T24M8.10 -
Arabidopsis thaliana, partial (11%)
Length = 701
Score = 80.1 bits (196), Expect = 6e-15
Identities = 49/132 (37%), Positives = 69/132 (52%), Gaps = 1/132 (0%)
Frame = +3
Query: 1254 GKLGDYNDGESNVQIPKDLLIYQSSNPIADIVHLMYPDIVQNVGCVKYYSDKAILAPTLD 1313
GK+ + ND + V P +PI IV YP++V ++ +AIL T +
Sbjct: 336 GKMSEPNDSYAEVDTPP-------GDPIDAIVQSTYPNLVSQYNNEQFLQSRAILTSTDE 494
Query: 1314 AVDSINQYALSLFPGNEKTYLSSDSVWRVDEDVGIEA-DWLTTEFLNGLKCSGMPDHKLV 1372
VD IN Y L L PG E+ S++ E ++A D + EFL LK S +P+HKL
Sbjct: 495 VVDQINDYVLKLIPGEERVIYSANR----SEVNDVQAFDAIPPEFLQSLKTSDLPNHKLT 662
Query: 1373 LKEGAPIMLMRN 1384
LK G PIML+R+
Sbjct: 663 LKVGTPIMLLRD 698
>BI271341
Length = 468
Score = 76.6 bits (187), Expect = 7e-14
Identities = 40/101 (39%), Positives = 64/101 (62%)
Frame = +1
Query: 1189 FRQILPVIPKGRRAEIVMSTINSSRLWRFCKVLNLTQNMRLTTSTCDGDNEEVKHFAKWV 1248
FR ILPVIP+G R++I+ +TINSS + C+V+ L +NM L + ++ E + F+K +
Sbjct: 1 FR*ILPVIPRGSRSDIIHATINSSCI*DHCQVVRLKKNMWLQQNGQSSNDPEFEQFSK*I 180
Query: 1249 LDMGDGKLGDYNDGESNVQIPKDLLIYQSSNPIADIVHLMY 1289
L +GDGK+ + ND +++ IP +LLI + + IV Y
Sbjct: 181 LKVGDGKIYEPNDSYADIDIPPELLISNYDDSLQTIVQSTY 303
>BG587143 weakly similar to PIR|A96586|A9 hypothetical protein F20D21.24
[imported] - Arabidopsis thaliana, partial (12%)
Length = 717
Score = 70.5 bits (171), Expect = 5e-12
Identities = 36/97 (37%), Positives = 50/97 (51%)
Frame = -3
Query: 510 YCLGTYTIEFQKRGLPHAHILLWLSMENKLRSPAMIDSVICAELPDPTQYPELFECVSNY 569
Y + IEFQKRGL HAHILLW ++ S +D +I AELP+ Q PE + V+ +
Sbjct: 601 YTTALHRIEFQKRGLRHAHILLWFGNSSRTPSSEEVDEIISAELPNKKQDPEAYNLVTKH 422
Query: 570 MVHGPWSMWIE**RFSLHEEWSVLQILPQKICFKNNI 606
M+HGP + I P+ C +NN+
Sbjct: 421 MIHGPCGV-----------------INPKSPCMENNV 362
>CB892166 similar to GP|20197614|gb unknown protein {Arabidopsis thaliana},
partial (3%)
Length = 748
Score = 57.4 bits (137), Expect = 4e-08
Identities = 29/53 (54%), Positives = 40/53 (74%)
Frame = -1
Query: 1468 YLPKPVFSHGQLYVAISRVKSRAGLKILICNEDTSQLDVTKNIVFQEVFQKIY 1520
+ + VFSHGQLYVAISRV SR GLKIL+ +E+ +D T N+V+ +VFQ ++
Sbjct: 289 FYDREVFSHGQLYVAISRVSSRNGLKILMIDENGDCIDNTTNVVY-KVFQNVW 134
>AJ497367 similar to GP|14140286|gb putative helicase {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 543
Score = 57.0 bits (136), Expect = 5e-08
Identities = 25/46 (54%), Positives = 38/46 (82%)
Frame = +1
Query: 1474 FSHGQLYVAISRVKSRAGLKILICNEDTSQLDVTKNIVFQEVFQKI 1519
FS+G+LYVA+SRV SR GLKIL+ +ED + ++ T N+V++EVF+ +
Sbjct: 19 FSNGKLYVAVSRVTSRKGLKILLAHEDGNCMNTTSNVVYKEVFRNL 156
>CA859513
Length = 363
Score = 43.9 bits (102), Expect = 5e-04
Identities = 22/61 (36%), Positives = 33/61 (54%)
Frame = +1
Query: 515 YTIEFQKRGLPHAHILLWLSMENKLRSPAMIDSVICAELPDPTQYPELFECVSNYMVHGP 574
Y EF+K LPHAH+L + D+ + AEL DP + L++ V + M+HGP
Sbjct: 139 YVAEFEKCDLPHAHMLFHRADN--------YDTFVSAELSDPVEQLRLYQTVVSVMIHGP 294
Query: 575 W 575
+
Sbjct: 295 Y 297
>TC84660 similar to PIR|D86481|D86481 hypothetical protein AAG28292.1
[imported] - Arabidopsis thaliana, partial (1%)
Length = 1009
Score = 40.8 bits (94), Expect = 0.004
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = +2
Query: 1461 SLSQVGVYLPKPVFSHGQLYVAISRVKSR 1489
S++ G+YLP+P+F HG LYVA+SRV SR
Sbjct: 68 SMATKGMYLPQPIF*HG*LYVALSRVTSR 154
>TC80146
Length = 476
Score = 38.5 bits (88), Expect = 0.020
Identities = 16/28 (57%), Positives = 23/28 (82%)
Frame = -3
Query: 1453 TINKSQGQSLSQVGVYLPKPVFSHGQLY 1480
TINKS+ QSLS + +YL +P+FSH ++Y
Sbjct: 450 TINKSR*QSLSYMKIYLSRPIFSHEEMY 367
>BE998307
Length = 624
Score = 34.3 bits (77), Expect = 0.38
Identities = 23/86 (26%), Positives = 45/86 (51%), Gaps = 1/86 (1%)
Frame = +2
Query: 1414 GTHIGKVVYISRMNLMPSDESMPVKFQRRQFPLIASFAMTINKSQGQSLSQVGVYL-PKP 1472
G + K +YI ++NL+ S ++ VKF+R + L + K + + ++ Y+ +
Sbjct: 365 GKMLDKNIYILQINLILSRANVSVKFKRHRLLLCFVLQWLLVKVKDKFYERLVYYIKTRS 544
Query: 1473 VFSHGQLYVAISRVKSRAGLKILICN 1498
VF+ QL+ ++ VK + K+ I N
Sbjct: 545 VFTDDQLHFDMNHVKCQGKSKV*ITN 622
>BQ137420
Length = 690
Score = 30.4 bits (67), Expect = 5.5
Identities = 16/45 (35%), Positives = 27/45 (59%)
Frame = +2
Query: 1414 GTHIGKVVYISRMNLMPSDESMPVKFQRRQFPLIASFAMTINKSQ 1458
G + K +YI ++NL+ S ++ VKF+R + L F M +KS+
Sbjct: 482 GKMLDKNIYILQINLILSGANVSVKFKRHRLLLGFGFTMVGSKSE 616
>TC83061 similar to GP|15292835|gb|AAK92786.1 unknown protein {Arabidopsis
thaliana}, partial (9%)
Length = 794
Score = 30.4 bits (67), Expect = 5.5
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = -2
Query: 492 LYAIHFYNQSIIYCHYLFYCLGTYTIEFQ 520
LY I F+N +II+ ++LF+ G YT +Q
Sbjct: 436 LYTITFFNFTIIFSYFLFF-FGLYTFSYQ 353
>TC90214 similar to SP|P55748|CP22_HORVU Serine carboxypeptidase II-2
precursor (EC 3.4.16.6) (CP-MII.2) (Fragment). [Barley],
partial (32%)
Length = 694
Score = 30.0 bits (66), Expect = 7.1
Identities = 23/89 (25%), Positives = 37/89 (40%)
Frame = -1
Query: 451 LPCFPYEIKSIHG*LQEGLFLWESCCRFVLCLCYII*YLISLYAIHFYNQSIIYCHYLFY 510
+PC P HG E ++ S C + +C Y Y+ N+ IY Y
Sbjct: 277 IPCTP----RAHGWKLESIYSIASRCYWDYSICIATEYP---YS*TSRNKFTIYVEYCSR 119
Query: 511 CLGTYTIEFQKRGLPHAHILLWLSMENKL 539
+ I GLP + +++W++M N L
Sbjct: 118 RILPVCIYHSAAGLPFSRLIIWINM*NPL 32
>TC84042 similar to PIR|T48214|T48214 endo-1 4-beta-mannosidase-like protein -
Arabidopsis thaliana, partial (37%)
Length = 684
Score = 30.0 bits (66), Expect = 7.1
Identities = 25/91 (27%), Positives = 38/91 (41%)
Frame = +3
Query: 1258 DYNDGESNVQIPKDLLIYQSSNPIADIVHLMYPDIVQNVGCVKYYSDKAILAPTLDAVDS 1317
+Y D ESN+ IP +L Y S A+ +VQ G +D+ V+
Sbjct: 108 EYEDWESNIHIPNSILTYGSEMEEAEW------QMVQKKGNQFVVNDQPFY------VNG 251
Query: 1318 INQYALSLFPGNEKTYLSSDSVWRVDEDVGI 1348
N Y L +F +E T V++ VG+
Sbjct: 252 FNTYWLMVFAADESTRGRVTQVFKHASSVGM 344
>AW688169 weakly similar to GP|21745054|gb| putative disease resistance gene
analog NBS-LRR {Malus prunifolia}, partial (16%)
Length = 540
Score = 29.6 bits (65), Expect = 9.3
Identities = 17/57 (29%), Positives = 30/57 (51%)
Frame = +2
Query: 1023 DVVEMSNLHDECFSKLNDGQLKIYQEIITAINANNGGFFFVYGYGGTGKTFLWKTLT 1079
DVV S+ D ++ D + + ++I+ A+ + +YG GG GKT L + +T
Sbjct: 326 DVVASSSTRD---GEMYDTRESLKKDIVKALGDSTSCNIGIYGLGGVGKTTLVEXVT 487
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.359 0.162 0.586
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,672,552
Number of Sequences: 36976
Number of extensions: 800317
Number of successful extensions: 9459
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 2972
Number of HSP's successfully gapped in prelim test: 507
Number of HSP's that attempted gapping in prelim test: 6278
Number of HSP's gapped (non-prelim): 3953
length of query: 1520
length of database: 9,014,727
effective HSP length: 109
effective length of query: 1411
effective length of database: 4,984,343
effective search space: 7032907973
effective search space used: 7032907973
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.8 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0233.16