BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0227.9
(273 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79458 weakly similar to PIR|D85436|D85436 MAP3K-like protein k... 297 3e-81
BQ147106 similar to GP|15451188|gb Unknown protein {Arabidopsis ... 156 7e-39
BQ149549 similar to PIR|B86270|B86 hypothetical protein AAF81295... 117 4e-27
TC93191 similar to GP|15451188|gb|AAK96865.1 Unknown protein {Ar... 78 4e-15
TC92169 similar to PIR|D85436|D85436 MAP3K-like protein kinase [... 51 4e-07
BI310444 similar to GP|15451188|gb| Unknown protein {Arabidopsis... 33 0.15
TC86001 homologue to GP|16508160|gb|AAL17948.1 phosphoinositide-... 30 0.99
TC79607 weakly similar to GP|16519227|gb|AAL25130.1 cellulose sy... 29 1.7
TC79606 weakly similar to GP|17381168|gb|AAL36396.1 putative cel... 29 1.7
TC83473 similar to GP|5852933|gb|AAD54273.1| dihydroflavonol-4-r... 28 3.8
TC86537 similar to GP|13377778|gb|AAK20858.1 fasciclin-like arab... 27 6.4
TC83118 similar to PIR|E96718|E96718 probable cullin T6C23.13 [i... 27 6.4
TC81125 similar to GP|17381108|gb|AAL36366.1 putative ubiquitin ... 27 8.4
BQ156610 OMNI|NTL01EC01 putative structural protein {Escherichia... 27 8.4
TC87399 similar to GP|17381168|gb|AAL36396.1 putative cellulose ... 27 8.4
>TC79458 weakly similar to PIR|D85436|D85436 MAP3K-like protein kinase
[imported] - Arabidopsis thaliana, partial (35%)
Length = 1088
Score = 297 bits (760), Expect = 3e-81
Identities = 139/252 (55%), Positives = 186/252 (73%), Gaps = 3/252 (1%)
Frame = +1
Query: 3 LPFNEYSWLTTHNSFAAKGVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDMWDYEDTI 62
LPFN YSWLTTHNSFA G +TGS +LA NQ+D+I DQLKNGVRG MLDM+D+++ +
Sbjct: 331 LPFNRYSWLTTHNSFAMAGARSATGSIILAPMNQDDTIADQLKNGVRGFMLDMYDFQNDV 510
Query: 63 WLCR---GPCTKYTTFQPALNVLKEVRVFLVTHPTEIITIFIDDHVTSGNGVNKVFDKAR 119
WLC G C +++F PA+N L+++R FL +P+EIITIFI+D+V + + KV +
Sbjct: 511 WLCHSTGGKCFNFSSFIPAVNALRDMRSFLDANPSEIITIFIEDYVRAPAALTKVIQASG 690
Query: 120 LRKFWFPVSKMPKNGSDWPTVKTMIRKNYRLIVFTSNASREASEGIAYEWNYVVESQFGN 179
+ K+ FPV ++PKNGSDWPTV MI N R I F+S +S+EA+EGI + W YVVE+Q+G+
Sbjct: 691 INKYMFPVGRLPKNGSDWPTVDDMILNNQRFIAFSSRSSKEAAEGIPFTWKYVVENQYGD 870
Query: 180 VGIKGGSCQNRPESLPMNNATKSLVLMNYFRNVRNHDDEACRDNSSPLIAMMHVCFRAAG 239
G++ GSC NR ES PMN ++SLVLMN+F + N +AC DNS+PL++M+ C AA
Sbjct: 871 EGMQPGSCPNRNESPPMNTKSRSLVLMNFFHSTPNR-SQACGDNSAPLLSMLKTCHEAAR 1047
Query: 240 NRWPNFIAVDFY 251
NRWPNFIAVD+Y
Sbjct: 1048NRWPNFIAVDYY 1083
>BQ147106 similar to GP|15451188|gb Unknown protein {Arabidopsis thaliana},
partial (36%)
Length = 662
Score = 156 bits (395), Expect = 7e-39
Identities = 74/138 (53%), Positives = 96/138 (68%), Gaps = 3/138 (2%)
Frame = +2
Query: 3 LPFNEYSWLTTHNSFAAKGVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDMWDYEDTI 62
LPFN+YSW+ THNSF+ G L F NQED++T+QL+NGVRGLMLDM+D+++ I
Sbjct: 248 LPFNKYSWIMTHNSFSIMDAPSLNGVQRLTFYNQEDTVTNQLRNGVRGLMLDMYDFQNDI 427
Query: 63 WLC---RGPCTKYTTFQPALNVLKEVRVFLVTHPTEIITIFIDDHVTSGNGVNKVFDKAR 119
WLC +G C +T FQPA+N LKEV FL +P EI+TI I+D+V + + +F A
Sbjct: 428 WLCHSFQGQCYNFTAFQPAINTLKEVEAFLTENPMEIVTIVIEDYVRTPKALINLFINAG 607
Query: 120 LRKFWFPVSKMPKNGSDW 137
L K+ FPVS MPKNG DW
Sbjct: 608 LDKYLFPVSDMPKNGEDW 661
>BQ149549 similar to PIR|B86270|B86 hypothetical protein AAF81295.1
[imported] - Arabidopsis thaliana, partial (29%)
Length = 635
Score = 117 bits (294), Expect = 4e-27
Identities = 53/100 (53%), Positives = 73/100 (73%), Gaps = 3/100 (3%)
Frame = +3
Query: 3 LPFNEYSWLTTHNSFAAKGVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDMWDYEDTI 62
LPFN+Y++LTTHN+FA +G TG P + TNQED+IT QL NGVR LMLD +D++D +
Sbjct: 336 LPFNKYAFLTTHNAFAIEGEPSHTGVPRITITNQEDTITQQLNNGVRALMLDTYDFDDDV 515
Query: 63 WLC---RGPCTKYTTFQPALNVLKEVRVFLVTHPTEIITI 99
WLC +G C +T F+PA++ LKE+ FL +P EI+T+
Sbjct: 516 WLCHSFQGHCHDFTAFEPAIDTLKEIEAFLSANPNEIVTL 635
>TC93191 similar to GP|15451188|gb|AAK96865.1 Unknown protein {Arabidopsis
thaliana}, partial (23%)
Length = 688
Score = 77.8 bits (190), Expect = 4e-15
Identities = 39/90 (43%), Positives = 55/90 (60%)
Frame = +2
Query: 178 GNVGIKGGSCQNRPESLPMNNATKSLVLMNYFRNVRNHDDEACRDNSSPLIAMMHVCFRA 237
G+ G+ ES P+N+ T SL L NYF + E+C++NS+PL M++ C++
Sbjct: 8 GDPGVHAVLAHIAKESKPLNSKTASLFLQNYFPTTPV-EAESCKENSAPLTDMVNTCYKT 184
Query: 238 AGNRWPNFIAVDFYKRGDGGGAPEALDLAN 267
AGN PNFIAV+FY R DGGG + +D N
Sbjct: 185 AGNVLPNFIAVNFYMRSDGGGVFDIVDRIN 274
>TC92169 similar to PIR|D85436|D85436 MAP3K-like protein kinase [imported] -
Arabidopsis thaliana, partial (6%)
Length = 505
Score = 51.2 bits (121), Expect = 4e-07
Identities = 21/30 (70%), Positives = 24/30 (80%)
Frame = +2
Query: 241 RWPNFIAVDFYKRGDGGGAPEALDLANRNL 270
RWPNFIAVD+Y R DGGG P+A+D AN L
Sbjct: 2 RWPNFIAVDYYLRSDGGGVPQAVDAANGRL 91
>BI310444 similar to GP|15451188|gb| Unknown protein {Arabidopsis thaliana},
partial (11%)
Length = 620
Score = 32.7 bits (73), Expect = 0.15
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = +3
Query: 244 NFIAVDFYKRGDGGGAPEALDLAN 267
NFIAV+FY R DGGG + +D N
Sbjct: 3 NFIAVNFYMRSDGGGVFDIVDKMN 74
>TC86001 homologue to GP|16508160|gb|AAL17948.1 phosphoinositide-specific
phospholipase C {Medicago truncatula}, partial (43%)
Length = 849
Score = 30.0 bits (66), Expect = 0.99
Identities = 22/69 (31%), Positives = 34/69 (48%), Gaps = 2/69 (2%)
Frame = +1
Query: 1 MELPFNEYSWLTTHNSFAAKGVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDMW--DY 58
M LP + Y T HNS+ TG+ L+ + I + L+ GVR + LD+W D
Sbjct: 310 MSLPLSHYYIFTGHNSYL-------TGNQ-LSSDCSDAPIIEALQRGVRVIELDIWPNDS 465
Query: 59 EDTIWLCRG 67
+D + + G
Sbjct: 466 KDDVDVLHG 492
>TC79607 weakly similar to GP|16519227|gb|AAL25130.1 cellulose synthase-like
protein OsCslE2 {Oryza sativa}, partial (17%)
Length = 670
Score = 29.3 bits (64), Expect = 1.7
Identities = 15/62 (24%), Positives = 28/62 (44%), Gaps = 1/62 (1%)
Frame = +2
Query: 69 CTKYTTFQPALNVLKEV-RVFLVTHPTEIITIFIDDHVTSGNGVNKVFDKARLRKFWFPV 127
CT +P + V+ V V +PTE +++++ D S + + ++ K W P
Sbjct: 329 CTANPDIEPPIMVINTVLSVMAYDYPTEKLSVYLSDDGGSDVTFYALLEASKFAKHWLPF 508
Query: 128 SK 129
K
Sbjct: 509 CK 514
>TC79606 weakly similar to GP|17381168|gb|AAL36396.1 putative cellulose
synthase catalytic subunit {Arabidopsis thaliana},
partial (17%)
Length = 688
Score = 29.3 bits (64), Expect = 1.7
Identities = 15/62 (24%), Positives = 28/62 (44%), Gaps = 1/62 (1%)
Frame = +1
Query: 69 CTKYTTFQPALNVLKEV-RVFLVTHPTEIITIFIDDHVTSGNGVNKVFDKARLRKFWFPV 127
CT +P + V+ V V +PTE +++++ D S + + ++ K W P
Sbjct: 352 CTANPEIEPPIMVINTVLSVMAYDYPTEKLSVYLSDDGGSDVTFYALLEASKFAKHWLPF 531
Query: 128 SK 129
K
Sbjct: 532 CK 537
>TC83473 similar to GP|5852933|gb|AAD54273.1| dihydroflavonol-4-reductase
DFR1 {Glycine max}, partial (96%)
Length = 1238
Score = 28.1 bits (61), Expect = 3.8
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = +1
Query: 1 MELPFNEYSWLTTHNSFAAKGVNWSTGSPVL 31
+ PF Y+W+ + FA++G+ S+G +L
Sbjct: 22 LSFPFKIYTWVQSQKQFASQGLQVSSGRGLL 114
>TC86537 similar to GP|13377778|gb|AAK20858.1 fasciclin-like
arabinogalactan-protein 2 {Arabidopsis thaliana}, partial
(57%)
Length = 1422
Score = 27.3 bits (59), Expect = 6.4
Identities = 14/37 (37%), Positives = 22/37 (58%)
Frame = -3
Query: 197 NNATKSLVLMNYFRNVRNHDDEACRDNSSPLIAMMHV 233
NNA +LV ++YF + +N +E + IA+MHV
Sbjct: 1375 NNAPNTLVSLSYFLHKQNQRNEEKNIYHNLTIAVMHV 1265
>TC83118 similar to PIR|E96718|E96718 probable cullin T6C23.13 [imported] -
Arabidopsis thaliana, partial (35%)
Length = 902
Score = 27.3 bits (59), Expect = 6.4
Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
Frame = +2
Query: 74 TFQPALNVLKEVRVFLVTHPTEIITIFIDDHVTSG-NGVNK 113
+FQ ALN E + L E I++F+DD + G GVN+
Sbjct: 188 SFQNALNSSFEYFINLNPRSPEFISLFVDDKLRKGLKGVNE 310
>TC81125 similar to GP|17381108|gb|AAL36366.1 putative ubiquitin
carboxyl-terminal hydrolase {Arabidopsis thaliana},
partial (34%)
Length = 820
Score = 26.9 bits (58), Expect = 8.4
Identities = 15/47 (31%), Positives = 24/47 (50%)
Frame = +1
Query: 169 WNYVVESQFGNVGIKGGSCQNRPESLPMNNATKSLVLMNYFRNVRNH 215
WNY + + G VG+K N+ + MN+ ++L + YFR H
Sbjct: 640 WNYDSKKETGYVGLK-----NQGATCYMNSLLQTLYHIPYFRKAVYH 765
>BQ156610 OMNI|NTL01EC01 putative structural protein {Escherichia coli
K12-MG1655}, partial (47%)
Length = 177
Score = 26.9 bits (58), Expect = 8.4
Identities = 11/27 (40%), Positives = 16/27 (58%)
Frame = +3
Query: 221 RDNSSPLIAMMHVCFRAAGNRWPNFIA 247
R SSPL++M CF ++G W + A
Sbjct: 60 RGFSSPLVSMSSSCFISSGKAWSRWYA 140
>TC87399 similar to GP|17381168|gb|AAL36396.1 putative cellulose synthase
catalytic subunit {Arabidopsis thaliana}, partial (53%)
Length = 1829
Score = 26.9 bits (58), Expect = 8.4
Identities = 15/62 (24%), Positives = 27/62 (43%), Gaps = 1/62 (1%)
Frame = +3
Query: 69 CTKYTTFQPALNVLKEV-RVFLVTHPTEIITIFIDDHVTSGNGVNKVFDKARLRKFWFPV 127
CT +P + V+ V V +P+E +++++ D S + + A K W P
Sbjct: 369 CTADPEIEPPMMVINTVLSVMAFDYPSEKLSVYLSDDGGSEITFYALLEAATFAKHWLPF 548
Query: 128 SK 129
K
Sbjct: 549 CK 554
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.136 0.430
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,293,828
Number of Sequences: 36976
Number of extensions: 124775
Number of successful extensions: 552
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 546
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 547
length of query: 273
length of database: 9,014,727
effective HSP length: 95
effective length of query: 178
effective length of database: 5,502,007
effective search space: 979357246
effective search space used: 979357246
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0227.9