BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0227.1
(556 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86719 similar to GP|599956|emb|CAA83434.1| chloroplast inner m... 55 9e-08
TC86854 homologue to PIR|T09538|T09538 acetyl-CoA carboxylase (E... 39 0.004
TC85559 similar to PIR|T07012|T07012 acetyl-CoA carboxylase (EC ... 30 1.9
TC76996 similar to GP|7670022|dbj|BAA94976.1 basic chitinase {Ar... 30 2.4
BQ137953 weakly similar to GP|22136724|gb unknown protein {Arabi... 29 5.4
BQ136417 weakly similar to EGAD|146423|156 vitellogenin {Anolis ... 29 5.4
BI267808 similar to GP|6729048|gb|A unknown protein 3' partial ... 29 5.4
TC85583 similar to PIR|T51335|T51335 subtilisin-like proteinase ... 28 7.1
>TC86719 similar to GP|599956|emb|CAA83434.1| chloroplast inner membrane
protein {Pisum sativum}, partial (63%)
Length = 2950
Score = 54.7 bits (130), Expect = 9e-08
Identities = 57/208 (27%), Positives = 93/208 (44%), Gaps = 15/208 (7%)
Frame = +2
Query: 39 DLLSQLQSHVHKALAGGGAEAV---KRNTSRNKLLPRERIDRLLDPGSSFLELSQLAGHE 95
D +S L++ KAL + N +R+ P +D + + F+EL
Sbjct: 650 DQISSLENKYQKALKNLYTHLTPIQRVNIARHPHRPT-CLDHIFNITDKFMELH--GDRN 820
Query: 96 LYEEPLPSGGVVTGIGPVHGRLCMFVANDPTVKG-----------GTYYPITVKKHLRAQ 144
Y++P +VTG+G + GR MF+ + KG G P +K LR
Sbjct: 821 GYDDP----AIVTGLGSIDGRTYMFIGHQ---KGRNTKESINRNFGMPTPHGYRKALRMM 979
Query: 145 EIASQCKLPCVYLVDSGGAFLPKQADVFPDRENFGR-IFYNQALMSAEGIPQIALVLGSC 203
E A P V +D+ GA+ AD+ + N G I +N M +P I++V+G
Sbjct: 980 EYADHHGFPVVTFIDTPGAY----ADLKSEELNQGEAIAHNLRSMFGLKVPVISIVIGEG 1147
Query: 204 TAGGAYIPAMADESVMVKGNGTIFLAGP 231
+GGA A++ +M++ NG ++A P
Sbjct: 1148GSGGALAIGCANKLLMLE-NGVFYVASP 1228
>TC86854 homologue to PIR|T09538|T09538 acetyl-CoA carboxylase (EC 6.4.1.2)
- alfalfa, partial (27%)
Length = 2193
Score = 39.3 bits (90), Expect = 0.004
Identities = 64/265 (24%), Positives = 98/265 (36%), Gaps = 32/265 (12%)
Frame = +1
Query: 196 IALVLGSCTAGGAYIPAMADESVMVKGNGTIFLAGPPLVKAATGEEV--SAEDLGGATVH 253
+ V G GAY+ + + + + I L G + G EV S LGG +
Sbjct: 265 LTYVTGRTVGIGAYLARLGMRCIQ-RLDQPIILTGFSALNKLLGREVYSSHMQLGGPKIM 441
Query: 254 CKTSGVSDYFAQDEFHALALGRNIIKNLHMAGKDVLANGLQNLSYEYKEPLYDVNEL--R 311
T+GV D+ ++ +I+K L V G + P +V L
Sbjct: 442 A-TNGVVHLTVSDDLEGVS---SILKWLSYVPSHV--GGALPIVKPLDPPEREVEYLPEN 603
Query: 312 SIAP-TDLKKQFDVRSV-IARIVDGSEFDEFKKLYGTTLVTGFARIYGQPVGIIG----- 364
S P + DV + I D F E + + T+VTG A++ G PVGI+
Sbjct: 604 SCDPRAAISGTLDVNGKWLGGIFDKDSFVETLEGWARTVVTGRAKLGGIPVGIVAVETQT 783
Query: 365 ---------------------NNGILFNESALKGAHFIEICTQRNIPLVFLQNITGFMVG 403
+ F +SA K A I + +PL + N GF G
Sbjct: 784 VMQIIPADPGQLDSHERVVPQAGQVWFPDSATKTAQAILDFNREELPLFIIANWRGFSGG 963
Query: 404 SRSEANGIAKSGAKMVMAVSCAKVP 428
R GI ++G+ +V + K P
Sbjct: 964 QRDLFEGILQAGSTIVENLRTYKQP 1038
>TC85559 similar to PIR|T07012|T07012 acetyl-CoA carboxylase (EC 6.4.1.2) -
potato chloroplast, partial (66%)
Length = 5011
Score = 30.4 bits (67), Expect = 1.9
Identities = 39/143 (27%), Positives = 55/143 (38%), Gaps = 1/143 (0%)
Frame = +3
Query: 106 VVTGIGPVHGRLCMFVANDPTVKGGTYYPITVKKHLRAQEIASQCKLPCVYLVDSGGAFL 165
V TG G V+G D GG+ + +K R E A+ +LP + + SGGA +
Sbjct: 3798 VQTGTGQVNGIPVAIGIMDFEFMGGSMGSVVGEKITRLIEYATNQRLPLIIVCASGGARM 3977
Query: 166 PKQADVFPDRENFGRIFYN-QALMSAEGIPQIALVLGSCTAGGAYIPAMADESVMVKGNG 224
+ + YN Q +P +L S T GG A ++ G
Sbjct: 3978 QEGSLSLMQMAKISASLYNYQINQKLFYVP----ILTSPTTGG----VTASFGML----G 4121
Query: 225 TIFLAGPPLVKAATGEEVSAEDL 247
I LA P A G+ V E L
Sbjct: 4122 DIILAEPDAYIAFAGKRVIEETL 4190
>TC76996 similar to GP|7670022|dbj|BAA94976.1 basic chitinase {Arabidopsis
thaliana}, partial (83%)
Length = 1185
Score = 30.0 bits (66), Expect = 2.4
Identities = 20/66 (30%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Frame = +1
Query: 213 MADESVMVKGNGTIFLAGPPLVKAATGEEVSAE--DLGGATVHCKTSGVSDYFAQDEFHA 270
+A +++V GN + PLVK G+++ + + G +V+C +SDYF +F
Sbjct: 52 LAIATILVIGNAQTSVK--PLVKTVKGKKLCDKGWECKGWSVYCCNETISDYFQTYQFEN 225
Query: 271 LALGRN 276
L RN
Sbjct: 226 LFSKRN 243
>BQ137953 weakly similar to GP|22136724|gb unknown protein {Arabidopsis
thaliana}, partial (21%)
Length = 657
Score = 28.9 bits (63), Expect = 5.4
Identities = 24/98 (24%), Positives = 48/98 (48%), Gaps = 1/98 (1%)
Frame = -1
Query: 389 IPLVFLQNITGFMVGSRSEANGIAKSGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGR 448
+PL F +N+TG +G S + G + S + ++ C+ SF A + G
Sbjct: 384 LPLPFPENLTGSPMGLAS-STGDSSSSSSSSSSLDCSS--------SSSFSASS---LGF 241
Query: 449 AYSPNFMFLW-PNARISVMGGAQAAGVLSQIEKTNKKK 485
+S ++ F++ ++R V+GG + S + TN+++
Sbjct: 240 IFSSSWSFIFLLSSR*EVVGGGALSSSSSLVVATNRRR 127
>BQ136417 weakly similar to EGAD|146423|156 vitellogenin {Anolis pulchellus},
partial (20%)
Length = 621
Score = 28.9 bits (63), Expect = 5.4
Identities = 24/98 (24%), Positives = 48/98 (48%), Gaps = 1/98 (1%)
Frame = -1
Query: 389 IPLVFLQNITGFMVGSRSEANGIAKSGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGR 448
+PL F +N+TG +G S + G + S + ++ C+ SF A + G
Sbjct: 384 LPLPFPENLTGSPMGLAS-STGDSSSSSSSSSSLDCSS--------SSSFSASS---LGF 241
Query: 449 AYSPNFMFLW-PNARISVMGGAQAAGVLSQIEKTNKKK 485
+S ++ F++ ++R V+GG + S + TN+++
Sbjct: 240 IFSSSWSFIFLLSSR*EVVGGGALSSSSSLVVATNRRR 127
>BI267808 similar to GP|6729048|gb|A unknown protein 3' partial {Arabidopsis
thaliana}, partial (8%)
Length = 414
Score = 28.9 bits (63), Expect = 5.4
Identities = 18/48 (37%), Positives = 25/48 (51%), Gaps = 5/48 (10%)
Frame = +1
Query: 475 LSQIEKTNKKKQ-----GIQWSKEEEEKFKTKVVEAYEVEGSPYYSTA 517
L+Q++ +N KK+ G QWSK E E+F EAY G + A
Sbjct: 211 LAQVKTSNGKKKLSDKLGSQWSKGELERF----YEAYRKHGKDWXKVA 342
>TC85583 similar to PIR|T51335|T51335 subtilisin-like proteinase AIR3
auxin-induced [imported] - Arabidopsis thaliana
(fragment), partial (40%)
Length = 2691
Score = 28.5 bits (62), Expect = 7.1
Identities = 21/53 (39%), Positives = 27/53 (50%), Gaps = 4/53 (7%)
Frame = +2
Query: 204 TAGGAYIPAMADESVMVKGNGTIFLAGPPLVKAATGEE----VSAEDLGGATV 252
TAGG ++P D SV GNGT+ G P + AT + + ED GA V
Sbjct: 722 TAGGNFVP---DASVFAIGNGTV-KGGSPRARVATYKVCWSLLDLEDCFGADV 868
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.137 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,273,247
Number of Sequences: 36976
Number of extensions: 197911
Number of successful extensions: 783
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 780
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 783
length of query: 556
length of database: 9,014,727
effective HSP length: 101
effective length of query: 455
effective length of database: 5,280,151
effective search space: 2402468705
effective search space used: 2402468705
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0227.1