BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0225.8
(423 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC91231 similar to GP|9502381|gb|AAF88088.1| T12C24.29 {Arabidop... 45 7e-05
CB892795 weakly similar to GP|21689663|gb| unknown protein {Arab... 42 3e-04
TC80138 similar to GP|14326493|gb|AAK60292.1 AT3g15070/K15M2_22 ... 42 4e-04
TC79610 similar to GP|16323294|gb|AAL15402.1 AT5g19430/F7K24_180... 42 6e-04
AW329822 similar to PIR|T00428|T004 hypothetical protein At2g475... 41 8e-04
TC77205 similar to GP|19347753|gb|AAL86301.1 putative RING-H2 zi... 41 8e-04
TC78411 similar to PIR|T47692|T47692 hypothetical protein T22E16... 41 8e-04
TC76690 similar to GP|21593806|gb|AAM65773.1 putative RING zinc ... 41 8e-04
TC89577 similar to GP|22795037|gb|AAN05420.1 putative RING prote... 40 0.001
BE942590 similar to GP|6850924|emb|C hypothetical protein {Cicer... 40 0.001
TC77620 similar to GP|10129643|emb|CAC08239. Pspzf zinc finger p... 40 0.001
TC79053 similar to GP|22795037|gb|AAN05420.1 putative RING prote... 40 0.001
BF645370 weakly similar to GP|12321864|gb| RING zinc finger prot... 40 0.001
TC78208 similar to PIR|H96703|H96703 probable RING zinc finger p... 40 0.002
TC93602 weakly similar to PIR|T51851|T51851 RING-H2 finger prote... 40 0.002
TC93425 similar to GP|15450675|gb|AAK96609.1 At1g26800/T24P13_21... 40 0.002
TC89939 similar to GP|15289911|dbj|BAB63606. hypothetical protei... 40 0.002
TC93528 similar to GP|20466490|gb|AAM20562.1 unknown protein {Ar... 40 0.002
TC87855 similar to GP|15081801|gb|AAK82555.1 AT5g41350/MYC6_6 {A... 39 0.003
TC86681 similar to GP|21553392|gb|AAM62485.1 unknown {Arabidopsi... 38 0.006
>TC91231 similar to GP|9502381|gb|AAF88088.1| T12C24.29 {Arabidopsis
thaliana}, partial (17%)
Length = 728
Score = 44.7 bits (104), Expect = 7e-05
Identities = 26/72 (36%), Positives = 34/72 (47%), Gaps = 7/72 (9%)
Frame = +1
Query: 294 IDGDQVDNVAGSPISN-----EWSCVICFE--DFSSTLGVLPCEHTFCLTCIQKWAHQRI 346
+ G ++ A SPI + + C IC D + L LPC H F TC+ KW H
Sbjct: 22 VGGIMIECQADSPIEHVLADEDAECCICLSAYDDGAELRQLPCGHHFHCTCVDKWLHIN- 198
Query: 347 SMGKISTCPLCK 358
+TCPLCK
Sbjct: 199 -----ATCPLCK 219
>CB892795 weakly similar to GP|21689663|gb| unknown protein {Arabidopsis
thaliana}, partial (10%)
Length = 586
Score = 42.4 bits (98), Expect = 3e-04
Identities = 22/51 (43%), Positives = 29/51 (56%), Gaps = 3/51 (5%)
Frame = +2
Query: 313 CVICFEDF--SSTLGVLP-CEHTFCLTCIQKWAHQRISMGKISTCPLCKAS 360
CVIC +F + T+G LP C +F L CIQ W + K CPLC++S
Sbjct: 272 CVICLNEFEDNDTIGTLPLCSQSFHLRCIQDW------LPKQPNCPLCRSS 406
>TC80138 similar to GP|14326493|gb|AAK60292.1 AT3g15070/K15M2_22
{Arabidopsis thaliana}, partial (20%)
Length = 649
Score = 42.0 bits (97), Expect = 4e-04
Identities = 18/50 (36%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = +3
Query: 312 SCVICFEDFSST--LGVLPCEHTFCLTCIQKWAHQRISMGKISTCPLCKA 359
SC+IC E+F + +G+L CEH + C+ KW + + CP+CK+
Sbjct: 234 SCIICQEEFKNKEKIGILRCEHEYHAECLTKWLLVK------NVCPICKS 365
>TC79610 similar to GP|16323294|gb|AAL15402.1 AT5g19430/F7K24_180
{Arabidopsis thaliana}, partial (54%)
Length = 789
Score = 41.6 bits (96), Expect = 6e-04
Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Frame = +1
Query: 312 SCVICFEDF--SSTLGVLPCEHTFCLTCIQKWAHQRISMGKISTCPLCKASFAWYKI 366
+C IC ++ T V CEH +C+TCI WA + + TCP CK F + +
Sbjct: 250 TCAICLDNIVLQETALVKGCEHAYCVTCILHWA----TYSQKVTCPQCKHPFEFLTV 408
>AW329822 similar to PIR|T00428|T004 hypothetical protein At2g47560
[imported] - Arabidopsis thaliana, partial (27%)
Length = 553
Score = 41.2 bits (95), Expect = 8e-04
Identities = 22/54 (40%), Positives = 27/54 (49%), Gaps = 3/54 (5%)
Frame = +1
Query: 309 NEWSCVICFEDFSS--TLGVLP-CEHTFCLTCIQKWAHQRISMGKISTCPLCKA 359
N C +C +F S T VLP C+H+F CI W H TCPLC+A
Sbjct: 235 NAPECAVCLSEFESGETGRVLPKCKHSFHTECIDMWFHSH------DTCPLCRA 378
>TC77205 similar to GP|19347753|gb|AAL86301.1 putative RING-H2 zinc finger
protein ATL6 {Arabidopsis thaliana}, partial (38%)
Length = 1317
Score = 41.2 bits (95), Expect = 8e-04
Identities = 19/51 (37%), Positives = 30/51 (58%), Gaps = 3/51 (5%)
Frame = +3
Query: 313 CVICFEDF--SSTLGVLP-CEHTFCLTCIQKWAHQRISMGKISTCPLCKAS 360
C +C +F S TL ++P C+H F CI +W +G +TCP+C+A+
Sbjct: 390 CAVCLNEFEESETLRLIPKCDHVFHPECIDEW------LGSHTTCPVCRAN 524
>TC78411 similar to PIR|T47692|T47692 hypothetical protein T22E16.190 -
Arabidopsis thaliana, partial (81%)
Length = 1378
Score = 41.2 bits (95), Expect = 8e-04
Identities = 43/163 (26%), Positives = 61/163 (37%), Gaps = 7/163 (4%)
Frame = +2
Query: 203 ILDLSPDD----RLCRMDRDRLHTEAAATSSLSGGVDVNDLQENSSGPDARLCNQNRAMN 258
+LD DD L +D D + T + T + V+ + SGP
Sbjct: 629 LLDREFDDLDYETLRALDSDNVSTAPSMTEEEINALPVHKYKV--SGPQK---------- 772
Query: 259 GSSDGIEQITDSNQIEDPQTVEQTSIDLCSSDGKFIDGDQVDNVAGSPISN-EWSCVICF 317
SS ++Q T S E Q D ++ V S S+ E +C +C
Sbjct: 773 -SSSSMQQATPSTSAEKTQ-------------------DNLNAVGNSKASDDELTCSVCL 892
Query: 318 EDFS--STLGVLPCEHTFCLTCIQKWAHQRISMGKISTCPLCK 358
E + L LPC H F +CI W Q+ TCP+CK
Sbjct: 893 EQVNVGDVLRSLPCLHQFHASCIDPWLRQQ------GTCPVCK 1003
>TC76690 similar to GP|21593806|gb|AAM65773.1 putative RING zinc finger
protein {Arabidopsis thaliana}, partial (22%)
Length = 1303
Score = 41.2 bits (95), Expect = 8e-04
Identities = 22/54 (40%), Positives = 27/54 (49%), Gaps = 3/54 (5%)
Frame = +3
Query: 309 NEWSCVICFEDFSS--TLGVLP-CEHTFCLTCIQKWAHQRISMGKISTCPLCKA 359
N C +C +F S T VLP C+H+F CI W H TCPLC+A
Sbjct: 369 NAPECAVCLSEFESGETGRVLPKCKHSFHTECIDMWFHSH------DTCPLCRA 512
>TC89577 similar to GP|22795037|gb|AAN05420.1 putative RING protein {Populus
x canescens}, partial (56%)
Length = 954
Score = 40.4 bits (93), Expect = 0.001
Identities = 20/59 (33%), Positives = 29/59 (48%), Gaps = 4/59 (6%)
Frame = +1
Query: 308 SNEWSCVICFEDFSSTLGVLPCEHTFCLTCIQKWAH----QRISMGKISTCPLCKASFA 362
SN + C IC DF++ V C H +C CI KW H + + CP+CK + +
Sbjct: 190 SNGFDCNICL-DFANEPVVTLCGHLYCWPCIYKWLHVQSDDSLGSDEHPQCPVCKENIS 363
>BE942590 similar to GP|6850924|emb|C hypothetical protein {Cicer arietinum},
complete
Length = 460
Score = 40.4 bits (93), Expect = 0.001
Identities = 21/49 (42%), Positives = 28/49 (56%), Gaps = 3/49 (6%)
Frame = +2
Query: 313 CVICFEDFSST--LGVLP-CEHTFCLTCIQKWAHQRISMGKISTCPLCK 358
CVIC D+ L ++P C HTF L+CI W + K STCP+C+
Sbjct: 146 CVICLADYKEREILRIMPKCGHTFHLSCIDIW------LRKQSTCPVCR 274
>TC77620 similar to GP|10129643|emb|CAC08239. Pspzf zinc finger protein-like
{Arabidopsis thaliana}, partial (26%)
Length = 1958
Score = 40.4 bits (93), Expect = 0.001
Identities = 19/50 (38%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Frame = +3
Query: 313 CVICFEDF--SSTLGVLPCEHTFCLTCIQKWAHQRISMGKISTCPLCKAS 360
C IC E++ +G L CEH + + CIQ+W + + CP+CKAS
Sbjct: 1614 CCICQEEYVEEDEVGSLLCEHKYHVVCIQQWLRLK------NWCPICKAS 1745
>TC79053 similar to GP|22795037|gb|AAN05420.1 putative RING protein {Populus
x canescens}, partial (28%)
Length = 1139
Score = 40.4 bits (93), Expect = 0.001
Identities = 23/71 (32%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Frame = +2
Query: 300 DNVAGSPISNEWSCVICFEDFSSTLGVLPCEHTFCLTCIQKWAHQRISMGKIS---TCPL 356
D S +N + C IC E + + L C H +C CI KW + + S + TCP+
Sbjct: 215 DTTISSGENNCFDCNICLESANDPVVTL-CGHLYCWPCIYKWLNVQSSSVEPDTQPTCPV 391
Query: 357 CKASFAWYKIV 367
CKA + +V
Sbjct: 392 CKAVISHTSLV 424
>BF645370 weakly similar to GP|12321864|gb| RING zinc finger protein;
17145-17621 {Arabidopsis thaliana}, partial (48%)
Length = 654
Score = 40.4 bits (93), Expect = 0.001
Identities = 24/55 (43%), Positives = 28/55 (50%), Gaps = 4/55 (7%)
Frame = +1
Query: 310 EWSCVICFEDF--SSTLGVLP-CEHTFCLTCIQKW-AHQRISMGKISTCPLCKAS 360
E C IC +F L VLP CEH F C+ KW HQ S+CPLC+ S
Sbjct: 400 ETECCICLGEFRDGEKLKVLPGCEHYFHCDCVDKWLTHQ-------SSCPLCRGS 543
>TC78208 similar to PIR|H96703|H96703 probable RING zinc finger protein
T23K23.8 [imported] - Arabidopsis thaliana, partial (72%)
Length = 1132
Score = 40.0 bits (92), Expect = 0.002
Identities = 20/48 (41%), Positives = 23/48 (47%), Gaps = 2/48 (4%)
Frame = +3
Query: 313 CVICFEDFS--STLGVLPCEHTFCLTCIQKWAHQRISMGKISTCPLCK 358
C IC + + L LPC H F TCI KW +TCPLCK
Sbjct: 978 CCICISPYEDEAELHALPCNHHFHSTCIVKWLKMN------ATCPLCK 1103
>TC93602 weakly similar to PIR|T51851|T51851 RING-H2 finger protein RHB1a
[imported] - Arabidopsis thaliana, partial (81%)
Length = 784
Score = 40.0 bits (92), Expect = 0.002
Identities = 29/122 (23%), Positives = 52/122 (41%), Gaps = 7/122 (5%)
Frame = +2
Query: 243 SSGPDARLCNQNRAMNG-----SSDGIEQITDSNQIEDPQTVEQTSIDLCSSDGKFIDGD 297
S+ PD+ C + + + + D IE+ +D E ++ ++L S+G
Sbjct: 377 SASPDSESCKETVSFSSFETSITHDDIEE-SDCKTQEKSASLSPRKVELSKSNG------ 535
Query: 298 QVDNVAGSPISNEWSCVICFEDFS--STLGVLPCEHTFCLTCIQKWAHQRISMGKISTCP 355
+ E C IC E++ + + CEH F L+CI +W M + +CP
Sbjct: 536 ----IQVLATEEEDGCPICLEEYDDDNPKNLTKCEHHFHLSCILEW------MERSDSCP 685
Query: 356 LC 357
+C
Sbjct: 686 IC 691
>TC93425 similar to GP|15450675|gb|AAK96609.1 At1g26800/T24P13_21
{Arabidopsis thaliana}, partial (28%)
Length = 596
Score = 39.7 bits (91), Expect = 0.002
Identities = 17/48 (35%), Positives = 27/48 (55%), Gaps = 2/48 (4%)
Frame = +2
Query: 313 CVICFEDFS--STLGVLPCEHTFCLTCIQKWAHQRISMGKISTCPLCK 358
CV+C E+F + +PC+H F CI+KW +G +CP+C+
Sbjct: 386 CVVCLEEFEVGGVVKEMPCKHRFHGKCIEKW------LGIHGSCPVCR 511
>TC89939 similar to GP|15289911|dbj|BAB63606. hypothetical protein~similar
to Arabidopsis thaliana chromosome 1 F18O14.3, partial
(23%)
Length = 978
Score = 39.7 bits (91), Expect = 0.002
Identities = 20/60 (33%), Positives = 28/60 (46%)
Frame = +2
Query: 308 SNEWSCVICFEDFSSTLGVLPCEHTFCLTCIQKWAHQRISMGKISTCPLCKASFAWYKIV 367
+ + C ICF D + + C H FC C+ KW H + CP+CKA K+V
Sbjct: 131 AGNFECNICF-DLAQDPIITLCGHLFCWPCLYKWLHFH---SQSRECPVCKAMIEEEKLV 298
>TC93528 similar to GP|20466490|gb|AAM20562.1 unknown protein {Arabidopsis
thaliana}, partial (24%)
Length = 755
Score = 39.7 bits (91), Expect = 0.002
Identities = 18/47 (38%), Positives = 25/47 (52%)
Frame = +3
Query: 313 CVICFEDFSSTLGVLPCEHTFCLTCIQKWAHQRISMGKISTCPLCKA 359
C IC E S + +L C+H FC C+ +W + TCPLC+A
Sbjct: 168 CAICQEKMHSPI-LLRCKHIFCEDCVSEWFERE------RTCPLCRA 287
>TC87855 similar to GP|15081801|gb|AAK82555.1 AT5g41350/MYC6_6 {Arabidopsis
thaliana}, partial (48%)
Length = 1194
Score = 39.3 bits (90), Expect = 0.003
Identities = 30/124 (24%), Positives = 53/124 (42%), Gaps = 4/124 (3%)
Frame = +1
Query: 238 DLQENSSGPDARLCNQNRAMNGSSDGIEQITDSNQIEDPQTVEQTSIDLCSSDGKFIDGD 297
++ + + + N N S D S + ++ + QT I+ ++ I+
Sbjct: 526 EISRDKNNTSSLSTNSNSVQEPSGDNHGTSPKSEEPKESECKGQTDIEQDTAKDSEIELS 705
Query: 298 QVDNVAGSPIS--NEWSCVICFEDFSSTLGVLP--CEHTFCLTCIQKWAHQRISMGKIST 353
++ G PI+ E +C IC E++ + L C H F L CI +W M + T
Sbjct: 706 KL----GEPINLVEEDTCPICLEEYDAENPKLTTQCGHDFHLACILEW------MERSET 855
Query: 354 CPLC 357
CP+C
Sbjct: 856 CPVC 867
>TC86681 similar to GP|21553392|gb|AAM62485.1 unknown {Arabidopsis
thaliana}, partial (14%)
Length = 607
Score = 38.1 bits (87), Expect = 0.006
Identities = 20/52 (38%), Positives = 25/52 (47%), Gaps = 2/52 (3%)
Frame = +2
Query: 309 NEWSCVICFEDFSSTLGV--LPCEHTFCLTCIQKWAHQRISMGKISTCPLCK 358
+E C IC + V LPC H F L C+ +W + IS CPLCK
Sbjct: 107 DEPECCICLAKYKDKEEVRQLPCSHMFHLKCVDQW------LRIISCCPLCK 244
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.133 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,588,587
Number of Sequences: 36976
Number of extensions: 201449
Number of successful extensions: 1114
Number of sequences better than 10.0: 146
Number of HSP's better than 10.0 without gapping: 1093
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1099
length of query: 423
length of database: 9,014,727
effective HSP length: 99
effective length of query: 324
effective length of database: 5,354,103
effective search space: 1734729372
effective search space used: 1734729372
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0225.8