BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0224.12
(426 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC93172 49 5e-06
BI270589 similar to PIR|C96829|C96 unknown protein F19K16.21 [im... 39 0.004
TC90741 similar to PIR|T51523|T51523 clpB heat shock protein-lik... 35 0.072
TC81629 similar to GP|15081654|gb|AAK82482.1 AT4g26750/F10M23_90... 34 0.094
TC89754 similar to GP|21618273|gb|AAM67323.1 unknown {Arabidopsi... 34 0.12
TC86042 similar to GP|20466712|gb|AAM20673.1 putative SF16 prote... 33 0.21
TC78017 similar to GP|16648718|gb|AAL25551.1 AT5g64130/MHJ24_11 ... 33 0.21
TC87688 similar to GP|10177535|dbj|BAB10930. gene_id:K1F13.21~un... 33 0.21
BQ141544 similar to GP|7022293|dbj| unnamed protein product {Hom... 33 0.27
BQ750941 similar to GP|46399|emb|CA ORF1 {Rhodococcus ruber}, pa... 33 0.27
TC85651 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis ... 32 0.36
TC89336 weakly similar to GP|21554135|gb|AAM63215.1 unknown {Ara... 32 0.47
TC92743 similar to GP|9759091|dbj|BAB09660.1 contains similarity... 32 0.47
AJ388952 similar to PIR|T04526|T045 hypothetical protein F16A16.... 31 0.80
BE940916 31 1.0
TC82626 weakly similar to PIR|G96798|G96798 hypothetical protein... 31 1.0
TC81365 similar to GP|14579399|gb|AAK69274.1 unknown {Glycine ma... 30 1.4
BQ151366 similar to GP|20146300|dbj hypothetical protein~similar... 30 1.4
TC82470 homologue to GP|23497460|gb|AAN37003.1 hypothetical prot... 30 1.4
TC78947 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis ... 30 2.3
>TC93172
Length = 861
Score = 48.5 bits (114), Expect = 5e-06
Identities = 40/205 (19%), Positives = 86/205 (41%), Gaps = 9/205 (4%)
Frame = +1
Query: 215 QAETTETAQSGGVADDSQIEEAFVQLKGLVFDAGFISGVRANSRLGQEVKELLAFL--LS 272
+ E TET I+ +L+ LVF + + + LG+EVK LL L +
Sbjct: 82 KXEATET-------QSESIKMLLDELRDLVFSRNLLKHLPNDVTLGEEVKALLVKLNYRA 240
Query: 273 QRLDQTRADALVELQTLLVDILATLDKAIAVEQLIETKQAQVTSNTNGLLPSKDEILKLT 332
L + ++ + + + + +D+ ++ + + L SK +I+K
Sbjct: 241 NELSEKQSSGITDFARIFTEATVNIDEGKLGNVTLQHLNVDHKDSMSKLQASKYKIMKFD 420
Query: 333 ARKALIADELSSVDARLDE-------LQREVAKLEKHKAMLIDEGPAVNSRLNTLAAECT 385
+ D++ + D +++ L+ + K+++ K+ L D + + E
Sbjct: 421 ESISAAEDKIKARDVEIEDIKAQIRLLEEKARKVQQEKSQLEDACSKCKEKRTEIVEEAK 600
Query: 386 NVMLSTAQLSKEVAAERRVKQSLDS 410
NV +T Q +++ ++ K+ LDS
Sbjct: 601 NVASTTIQTREKIDNLKKKKRELDS 675
>BI270589 similar to PIR|C96829|C96 unknown protein F19K16.21 [imported] -
Arabidopsis thaliana, partial (20%)
Length = 651
Score = 38.9 bits (89), Expect = 0.004
Identities = 39/146 (26%), Positives = 65/146 (43%), Gaps = 2/146 (1%)
Frame = +3
Query: 271 LSQRLDQTRADALVELQTLLVDILATLDKAIAVEQLIETKQAQVTSNTNGLLPSKDEILK 330
L Q L + A+ + L DI + A E+ E QV +T LL + +
Sbjct: 33 LRQTLSRKEQQAVFKEDMLCRDIEDLQKRYQASERRCEELITQVPESTRPLLRQIEAMQD 212
Query: 331 LTARKALI-ADELSSVDARLDELQREVAKLEKHKAMLIDEGPAVNSRLNTLAAECTNVML 389
AR+A A ++++RL E + + A E+ + + D SR+N L A+ + +
Sbjct: 213 SNARRAEAWAAVERTLNSRLQEAEAKAATAEERERSVNDRLSQTLSRINVLEAQISCLRA 392
Query: 390 STAQLSKEVAAER-RVKQSLDSRIAA 414
QLS+ + ER R +S +AA
Sbjct: 393 EQTQLSRTLEKERQRAAESRQEYLAA 470
>TC90741 similar to PIR|T51523|T51523 clpB heat shock protein-like -
Arabidopsis thaliana, partial (24%)
Length = 710
Score = 34.7 bits (78), Expect = 0.072
Identities = 23/71 (32%), Positives = 35/71 (48%), Gaps = 1/71 (1%)
Frame = +1
Query: 341 ELSSVDARLDELQREVAKLEKHKAMLI-DEGPAVNSRLNTLAAECTNVMLSTAQLSKEVA 399
E++S LDE+ R V KLE + L+ D A RLN L E + + +L+++
Sbjct: 142 EITSKPTALDEINRSVLKLEMERLSLMNDTDKASKDRLNRLETELSLLKEKQGELTEQWE 321
Query: 400 AERRVKQSLDS 410
E+ V L S
Sbjct: 322 HEKSVMTRLQS 354
>TC81629 similar to GP|15081654|gb|AAK82482.1 AT4g26750/F10M23_90
{Arabidopsis thaliana}, partial (19%)
Length = 824
Score = 34.3 bits (77), Expect = 0.094
Identities = 25/88 (28%), Positives = 35/88 (39%)
Frame = +2
Query: 79 PPQDMQNMPSPAREENVSHDEVQGSEGQEPPIIQPDQVIGLMPPPKADVLVSTHSEPSSR 138
PP Q+ P P ++ H + Q PP P Q PP ++D S++SEP +
Sbjct: 68 PPPPSQDYPPPPSQDY--HPPPPSQDYQPPP---PSQDYHQPPPARSD---SSYSEPYNH 223
Query: 139 QLQTSPQQPQPSPQQPRQGARVALSSPH 166
Q + Q P P S PH
Sbjct: 224 QQYSPDQSQNLGPNYPSHETPPPYSLPH 307
>TC89754 similar to GP|21618273|gb|AAM67323.1 unknown {Arabidopsis
thaliana}, partial (88%)
Length = 759
Score = 33.9 bits (76), Expect = 0.12
Identities = 22/64 (34%), Positives = 29/64 (44%), Gaps = 3/64 (4%)
Frame = +2
Query: 121 PPPKADVLVSTHSEPS---SRQLQTSPQQPQPSPQQPRQGARVALSSPHAKGGSASSKTA 177
PPP + ST S S S S PSP P + + SSP + ASS T+
Sbjct: 110 PPPHQSIPSSTSSASSPSPSSAHHASVSNSHPSPSPPPTSSSPSSSSPTSWSSPASSTTS 289
Query: 178 ASSH 181
+S+H
Sbjct: 290 SSNH 301
>TC86042 similar to GP|20466712|gb|AAM20673.1 putative SF16 protein
{Arabidopsis thaliana}, partial (39%)
Length = 2305
Score = 33.1 bits (74), Expect = 0.21
Identities = 40/173 (23%), Positives = 63/173 (36%)
Frame = +1
Query: 115 QVIGLMPPPKADVLVSTHSEPSSRQLQTSPQQPQPSPQQPRQGARVALSSPHAKGGSASS 174
QV+ + PP + + P SP+ P P P PR SP A +S
Sbjct: 613 QVLAIRPPTPPE---RPKTPPFVPPRVASPRPPSPKPPSPRD------PSPRAASPRVTS 765
Query: 175 KTAASSHTSGERLSALLVEDPLSIFQSFFDGTLDLESPPRQAETTETAQSGGVADDSQIE 234
AASS + +P L++ A ++A G +A
Sbjct: 766 PKAASSRNVHQHKEVRYRPEP------------TLQNQHVSATKIQSAYRGYMA-----R 894
Query: 235 EAFVQLKGLVFDAGFISGVRANSRLGQEVKELLAFLLSQRLDQTRADALVELQ 287
++F LKGLV G + G + +K + + Q Q+R ++E Q
Sbjct: 895 KSFRALKGLVRLQGVVRGQNVKRQTVNAMKHMQLLVRVQSQIQSRRIQMLENQ 1053
>TC78017 similar to GP|16648718|gb|AAL25551.1 AT5g64130/MHJ24_11
{Arabidopsis thaliana}, partial (67%)
Length = 887
Score = 33.1 bits (74), Expect = 0.21
Identities = 29/100 (29%), Positives = 44/100 (44%), Gaps = 4/100 (4%)
Frame = +3
Query: 85 NMPSPAREENVSHDEVQGSEGQEPPIIQPDQVIGLMPPPKADVLVSTHSEPSSRQLQTSP 144
NMPSP ++E + G ++PP+I D + S+P L+
Sbjct: 207 NMPSPQKQEETLKKKYGGIVPKKPPLISKDHERAYFDSADWALGKQGGSKPKG-PLEALR 383
Query: 145 QQPQPSPQQPRQGARVALSSPHA----KGGSASSKTAASS 180
+ QP+ QQ R SP+A +GGS S+ AAS+
Sbjct: 384 PKLQPTQQQTRY-----RKSPYAPSGEEGGSVPSEDAASN 488
>TC87688 similar to GP|10177535|dbj|BAB10930. gene_id:K1F13.21~unknown
protein {Arabidopsis thaliana}, partial (46%)
Length = 2077
Score = 33.1 bits (74), Expect = 0.21
Identities = 22/47 (46%), Positives = 29/47 (60%), Gaps = 6/47 (12%)
Frame = -2
Query: 13 SSKPPPIAL-----SSSSSSLESSSSSLNSTE-SDPVWDKLTFYFNS 53
SS PPP+ SSSSSSL SSSSS +S++ S P F+F++
Sbjct: 576 SSIPPPLTFSFSSSSSSSSSLPSSSSSSSSSKSSSPSLPPFAFFFST 436
>BQ141544 similar to GP|7022293|dbj| unnamed protein product {Homo sapiens},
partial (5%)
Length = 1284
Score = 32.7 bits (73), Expect = 0.27
Identities = 26/81 (32%), Positives = 34/81 (41%)
Frame = -1
Query: 100 VQGSEGQEPPIIQPDQVIGLMPPPKADVLVSTHSEPSSRQLQTSPQQPQPSPQQPRQGAR 159
+Q S G PP I P + L P P+ V S S + P+P PR+G
Sbjct: 570 LQQSPGASPPSIHPS--LRLPPTPRPPVAPS------------SVRPPRPLASPPRRGPG 433
Query: 160 VALSSPHAKGGSASSKTAASS 180
V P A+ GS S + SS
Sbjct: 432 VVARLPIARRGSFSCLNSLSS 370
>BQ750941 similar to GP|46399|emb|CA ORF1 {Rhodococcus ruber}, partial (12%)
Length = 743
Score = 32.7 bits (73), Expect = 0.27
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +1
Query: 122 PPKADVLVSTHSEPSSRQLQTSPQQPQPSPQQPRQ 156
P AD+ T + P S++ SPQ P P+P+ PR+
Sbjct: 178 PATADLQGPTGTTPRSKRCTRSPQSPSPTPRLPRR 282
>TC85651 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis thaliana,
partial (39%)
Length = 1269
Score = 32.3 bits (72), Expect = 0.36
Identities = 24/93 (25%), Positives = 38/93 (40%), Gaps = 13/93 (13%)
Frame = +3
Query: 49 FYFNSKPIAWQHPGCEPYYTKECRDNLSNFPPQ---------DMQNMPSPAREENVSHDE 99
F+ + P+ +HP P + RDNL ++ + + + + +
Sbjct: 429 FFIKTFPLHTRHPHLIPCIPQPFRDNLESYSLEVNKLCVTLIEFMSKALKIKPNELLDIF 608
Query: 100 VQGSEGQE----PPIIQPDQVIGLMPPPKADVL 128
+GS+ PP QPDQVIGL P A L
Sbjct: 609 EEGSQAMRMNYYPPCPQPDQVIGLNPHSDAGTL 707
>TC89336 weakly similar to GP|21554135|gb|AAM63215.1 unknown {Arabidopsis
thaliana}, partial (7%)
Length = 1241
Score = 32.0 bits (71), Expect = 0.47
Identities = 32/140 (22%), Positives = 59/140 (41%), Gaps = 7/140 (5%)
Frame = +1
Query: 294 LATLDKAIAVEQLIETKQAQVTSNTNGLLPSKDEILKLTARKALIADELSSVDARLDELQ 353
++ L ++ E++I+ K S G L S + L L DEL ++ ELQ
Sbjct: 511 VSNLKLKLSEEEIIKLKNQNEKSE--GQLDSVQKELTLNM------DELEHKKGQVLELQ 666
Query: 354 REVAKLEKHKAMLIDEGPAVNSRLN-------TLAAECTNVMLSTAQLSKEVAAERRVKQ 406
++ A+LE H L+++ N L L E + + T QL ++ +
Sbjct: 667 KQKAELETHVPNLVEQLEVANEHLKISNDEVARLRKELESNLAETRQLQDQLEVAQENVT 846
Query: 407 SLDSRIAAAGVQLEAFKSRI 426
L+ ++ + Q+ + RI
Sbjct: 847 KLEWQLDSGRKQIRELEDRI 906
>TC92743 similar to GP|9759091|dbj|BAB09660.1 contains similarity to unknown
protein~gb|AAF47871.1~gene_id:MJJ3.6 {Arabidopsis
thaliana}, partial (14%)
Length = 662
Score = 32.0 bits (71), Expect = 0.47
Identities = 27/96 (28%), Positives = 33/96 (34%)
Frame = +3
Query: 121 PPPKADVLVSTHSEPSSRQLQTSPQQPQPSPQQPRQGARVALSSPHAKGGSASSKTAASS 180
PPP T S TSP PQP P P S P S +S + +S
Sbjct: 84 PPPPHSSATQTSPNQYSNLTSTSPATPQPMPPPPTSPRSNPSSPPPPPALSPASSASNAS 263
Query: 181 HTSGERLSALLVEDPLSIFQSFFDGTLDLESPPRQA 216
+ + A P SI F G + L P A
Sbjct: 264 NPPIQHGLAPPSASPSSISSVFKAGLVKLLISPLHA 371
Score = 28.9 bits (63), Expect = 3.9
Identities = 19/73 (26%), Positives = 26/73 (35%)
Frame = +3
Query: 79 PPQDMQNMPSPAREENVSHDEVQGSEGQEPPIIQPDQVIGLMPPPKADVLVSTHSEPSSR 138
PP SP + N++ + PP P PPP A S+ S S+
Sbjct: 90 PPHSSATQTSPNQYSNLTSTSPATPQPMPPPPTSPRSNPSSPPPPPALSPASSASNASNP 269
Query: 139 QLQTSPQQPQPSP 151
+Q P SP
Sbjct: 270 PIQHGLAPPSASP 308
>AJ388952 similar to PIR|T04526|T045 hypothetical protein F16A16.160 -
Arabidopsis thaliana, partial (24%)
Length = 591
Score = 31.2 bits (69), Expect = 0.80
Identities = 20/31 (64%), Positives = 21/31 (67%)
Frame = +3
Query: 7 PRAMWFSSKPPPIALSSSSSSLESSSSSLNS 37
PRAM SS P P + SSSSSS SSSSS S
Sbjct: 198 PRAMSSSSSPSPSSSSSSSSS--SSSSSFGS 284
>BE940916
Length = 632
Score = 30.8 bits (68), Expect = 1.0
Identities = 29/123 (23%), Positives = 50/123 (40%), Gaps = 5/123 (4%)
Frame = +1
Query: 128 LVSTHSEPSSRQLQTSPQQPQPSPQQPRQGARVALSSPHAKGGSASSKTAASSHTSGERL 187
+V T ++P+++Q PQQP PQ+P +V+ + S G+
Sbjct: 121 IVPTPTKPTTQQEPQKPQQPATIPQKPNTAPQVSDQKKQVVQQEPKKEIEEVSQRKGKGK 300
Query: 188 SALLVEDPLSIFQSFFD-----GTLDLESPPRQAETTETAQSGGVADDSQIEEAFVQLKG 242
+ L ED + +L +E RQ + A G+ D+ + ++ V L
Sbjct: 301 AEELKEDIDDVNNKVASKVAEKKSLTMEMVSRQRQEYVNALC-GLVDEVKKQKKLVNLLV 477
Query: 243 LVF 245
LVF
Sbjct: 478 LVF 486
>TC82626 weakly similar to PIR|G96798|G96798 hypothetical protein F22K20.8
[imported] - Arabidopsis thaliana, partial (14%)
Length = 660
Score = 30.8 bits (68), Expect = 1.0
Identities = 27/77 (35%), Positives = 39/77 (50%), Gaps = 5/77 (6%)
Frame = +3
Query: 107 EPPIIQPDQV----IGLMPPPKADVLVSTHSEP-SSRQLQTSPQQPQPSPQQPRQGARVA 161
EP +IQ + I L+ K D+ H +P +S L S QQPQ +QPR +
Sbjct: 33 EPCLIQQENTTNPRILLICTMKPDM---KHGKPXNSNNLWPSTQQPQLDDEQPRW----S 191
Query: 162 LSSPHAKGGSASSKTAA 178
++SPH + S S+T A
Sbjct: 192 MNSPHYRSLSPVSRTEA 242
>TC81365 similar to GP|14579399|gb|AAK69274.1 unknown {Glycine max}, partial
(38%)
Length = 920
Score = 30.4 bits (67), Expect = 1.4
Identities = 22/43 (51%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
Frame = -1
Query: 12 FSSKPPPIALSSSSSSLESS--SSSLNSTESDPVWDKLTFYFN 52
F PPP SSSSSS SS SSS +S+ S P L F FN
Sbjct: 383 FEP*PPPSPDSSSSSSESSSHSSSSSSSSSSSPPPPFLPFSFN 255
>BQ151366 similar to GP|20146300|dbj hypothetical protein~similar to Oryza
sativa OSJNBa0058E19.20, partial (9%)
Length = 1137
Score = 30.4 bits (67), Expect = 1.4
Identities = 18/59 (30%), Positives = 29/59 (48%), Gaps = 1/59 (1%)
Frame = +2
Query: 132 HSEPSSRQLQTSPQQPQPSPQQPRQGARVAL-SSPHAKGGSASSKTAASSHTSGERLSA 189
H+ P++R +P+QP P+P +PR V + + H + K A TS E +A
Sbjct: 581 HARPAAR----APRQPPPNPTEPRAATCVIIRARTHGANQR*TEKEPAKRRTSNEEENA 745
>TC82470 homologue to GP|23497460|gb|AAN37003.1 hypothetical protein
{Plasmodium falciparum 3D7}, partial (8%)
Length = 1064
Score = 30.4 bits (67), Expect = 1.4
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = -3
Query: 19 IALSSSSSSLESSSSSLNSTESDPVWDKLTFY 50
+ +SSSSSS SSSSS +S+ S V D L F+
Sbjct: 99 VDMSSSSSSSSSSSSSSSSSSSYGVGDTLDFF 4
>TC78947 similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis thaliana,
partial (40%)
Length = 1031
Score = 29.6 bits (65), Expect = 2.3
Identities = 27/86 (31%), Positives = 34/86 (39%), Gaps = 13/86 (15%)
Frame = +2
Query: 49 FYFNSKPIAWQHPGCEPYYTKECRDNLSNFPPQ----------DMQNMPSPAREENVS-- 96
FY N+ P +HP P K RD+L N+ + M+ E V
Sbjct: 419 FYINTFPPDARHPHLIPNIPKPFRDHLENYCLELKKLAVTIIGRMEKALKIKSNELVEFF 598
Query: 97 HDEVQGSE-GQEPPIIQPDQVIGLMP 121
D QG PP QP+ VIGL P
Sbjct: 599 EDIYQGMRMNYYPPCPQPEHVIGLKP 676
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.126 0.342
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,387,057
Number of Sequences: 36976
Number of extensions: 194625
Number of successful extensions: 2371
Number of sequences better than 10.0: 94
Number of HSP's better than 10.0 without gapping: 1584
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2012
length of query: 426
length of database: 9,014,727
effective HSP length: 99
effective length of query: 327
effective length of database: 5,354,103
effective search space: 1750791681
effective search space used: 1750791681
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0224.12