BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0220.1
(751 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC84709 similar to GP|16326133|dbj|BAB70510. Myb {Nicotiana taba... 32 0.69
TC90066 similar to GP|9369388|gb|AAF87136.1| T23E23.3 {Arabidops... 31 2.0
TC84294 homologue to GP|168765|gb|AAA33563.1|| plasma membrane H... 31 2.0
TC78059 similar to GP|4206787|gb|AAD11808.1| syntaxin-related pr... 30 2.6
BQ752404 30 3.4
TC88385 similar to GP|22136512|gb|AAM91334.1 unknown protein {Ar... 30 4.5
TC92743 similar to GP|9759091|dbj|BAB09660.1 contains similarity... 30 4.5
TC81882 similar to GP|9759349|dbj|BAB10004.1 contains similarity... 30 4.5
TC87220 similar to GP|9758584|dbj|BAB09197.1 gb|AAC80623.1~gene_... 29 5.8
CA923192 similar to GP|20196906|gb| expressed protein {Arabidops... 29 5.8
TC88705 similar to GP|10177834|dbj|BAB11263. gene_id:MCD7.9~unkn... 29 7.6
TC87714 similar to PIR|S22697|S22697 extensin - Volvox carteri (... 29 7.6
TC76369 similar to PIR|T52336|T52336 probable type 1 membrane pr... 29 7.6
TC89281 similar to GP|11994197|dbj|BAB01300. emb|CAA18500.1~gene... 29 7.6
TC84713 28 9.9
BQ751262 similar to GP|6715100|gb|A versicolorin B synthase {Asp... 28 9.9
TC77527 similar to GP|9293881|dbj|BAB01784.1 hydroxyproline-rich... 28 9.9
TC79504 weakly similar to GP|21734794|gb|AAM76972.1 reduced vern... 28 9.9
>TC84709 similar to GP|16326133|dbj|BAB70510. Myb {Nicotiana tabacum},
partial (8%)
Length = 392
Score = 32.3 bits (72), Expect = 0.69
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = +2
Query: 588 DAVNSLIFTIAQHFVGDPSLIKDRFGDLLSNLKCKSLG 625
D+ +L+ + A+ F G PS++K R DLLS L K +G
Sbjct: 134 DSPEALLKSAAKTFAGTPSILKKRCRDLLSPLSDKRIG 247
>TC90066 similar to GP|9369388|gb|AAF87136.1| T23E23.3 {Arabidopsis
thaliana}, partial (33%)
Length = 1034
Score = 30.8 bits (68), Expect = 2.0
Identities = 19/70 (27%), Positives = 25/70 (35%)
Frame = +3
Query: 7 YFLRTNCEKSGETTLFLTDYDKANVIVPKTIQWSDITLPDKWSLEKAAPAQPVETPEFRE 66
YFLR N + ET+ D D W + LP S +A +E E
Sbjct: 342 YFLRWNSDSEWETSHLFADLD-----------WKQVVLPVMQSYTEATDGSNIEVKESAL 488
Query: 67 IIQHQSGQVD 76
+ HQ D
Sbjct: 489 VWHHQDADPD 518
>TC84294 homologue to GP|168765|gb|AAA33563.1|| plasma membrane H+ ATPase
{Neurospora crassa}, partial (29%)
Length = 815
Score = 30.8 bits (68), Expect = 2.0
Identities = 22/92 (23%), Positives = 41/92 (43%), Gaps = 8/92 (8%)
Frame = +2
Query: 399 VIPETPEPTPEAVSNPAV--------SAPAPPKTRTSTRNTSTSLNNIEDGKVESDSEDQ 450
++P P P V P+ + PAP STR++S+S + ++ S
Sbjct: 134 LLPAWPLPARRRVWTPSTRPS*SLCATTPAPSPFCPSTRSSSSSPSTPSPRRLPPSSNPP 313
Query: 451 SVKSIPVNTVIHNSRNQWRKETKLYYPRATAP 482
+V+ +P + V+ + ++ T L R+T P
Sbjct: 314 TVRRLPASRVLPSLSSRPSSRTTLSPRRSTRP 409
>TC78059 similar to GP|4206787|gb|AAD11808.1| syntaxin-related protein
Nt-syr1 {Nicotiana tabacum}, partial (94%)
Length = 1595
Score = 30.4 bits (67), Expect = 2.6
Identities = 18/51 (35%), Positives = 30/51 (58%)
Frame = -2
Query: 385 LLQKVVEQLQLLNQVIPETPEPTPEAVSNPAVSAPAPPKTRTSTRNTSTSL 435
LL KVV+ + + +++P+T ++ S+P +TR+STR T TSL
Sbjct: 619 LLSKVVKAIHGILKLLPQT--------THHRCSSPVRGRTRSSTRQTPTSL 491
>BQ752404
Length = 613
Score = 30.0 bits (66), Expect = 3.4
Identities = 27/101 (26%), Positives = 44/101 (42%), Gaps = 21/101 (20%)
Frame = -2
Query: 400 IPET-PEPTPEAVSNPAVSAPAPPKTRTSTRNTSTSLNNI---------------EDGKV 443
+PET P PT P V P +T + +T+L+ + E +
Sbjct: 324 LPETRPAPTAATTVVPTVPETRPRRTSILSDTLATNLDRMLCRITNATSAKRYTPEALQT 145
Query: 444 ESDSEDQSVKSIPVNTVI-----HNSRNQWRKETKLYYPRA 479
E + S++S P TV+ +SRNQ R+ ++ Y PR+
Sbjct: 144 EPSARSASIRSAP--TVLA*SHARSSRNQTRRFSRAYKPRS 28
>TC88385 similar to GP|22136512|gb|AAM91334.1 unknown protein {Arabidopsis
thaliana}, partial (71%)
Length = 1001
Score = 29.6 bits (65), Expect = 4.5
Identities = 17/54 (31%), Positives = 27/54 (49%), Gaps = 5/54 (9%)
Frame = +2
Query: 548 GQLKGWWDN-----YLTEDEKNQILTAIKTDEEGNPIIEDGKFILDAVNSLIFT 596
G++K W+ N LT +N + +KTDE+ I G F DA ++F+
Sbjct: 473 GEIKEWYPNNPVLRVLTPALRNLFVYPVKTDEQNIYISITGGFKSDAATEILFS 634
>TC92743 similar to GP|9759091|dbj|BAB09660.1 contains similarity to unknown
protein~gb|AAF47871.1~gene_id:MJJ3.6 {Arabidopsis
thaliana}, partial (14%)
Length = 662
Score = 29.6 bits (65), Expect = 4.5
Identities = 25/108 (23%), Positives = 42/108 (38%), Gaps = 17/108 (15%)
Frame = +3
Query: 365 SNRSRHSAQTLRAKNESDKELLQKVVE--------------QLQLLNQVIPETPEPTPEA 410
+NR RH T+R ++ + V+ Q L P TP+P P
Sbjct: 3 NNRWRHHITTVRHRHNHFLTPIPTAVDPPPPHSSATQTSPNQYSNLTSTSPATPQPMPPP 182
Query: 411 VSNPA--VSAPAPPKTRTSTRNTSTSLN-NIEDGKVESDSEDQSVKSI 455
++P S+P PP + + S + N I+ G + S+ S+
Sbjct: 183 PTSPRSNPSSPPPPPALSPASSASNASNPPIQHGLAPPSASPSSISSV 326
>TC81882 similar to GP|9759349|dbj|BAB10004.1 contains similarity to unknown
protein~gb|AAF72944.1~gene_id:MAH20.11 {Arabidopsis
thaliana}, partial (5%)
Length = 875
Score = 29.6 bits (65), Expect = 4.5
Identities = 28/91 (30%), Positives = 40/91 (43%), Gaps = 4/91 (4%)
Frame = +3
Query: 400 IPETPEPTPEAVSNPAVSAPAPPKTRTSTRNTSTSLNNIEDGKVESD---SEDQSVKSIP 456
+P P P P NPA+SA P +T T+T SL N +++ S + KS P
Sbjct: 87 LPNNP*PPPCPPPNPAISAAEPTQTPTTTL-LPPSLPNPPPLNLKNPPNFSVSPTTKSTP 263
Query: 457 V-NTVIHNSRNQWRKETKLYYPRATAPDLLL 486
+ ++H N P TAP+ LL
Sbjct: 264 ITKPLVHAPPN----------PTTTAPNPLL 326
>TC87220 similar to GP|9758584|dbj|BAB09197.1
gb|AAC80623.1~gene_id:MBD2.15~similar to unknown protein
{Arabidopsis thaliana}, partial (17%)
Length = 2008
Score = 29.3 bits (64), Expect = 5.8
Identities = 16/64 (25%), Positives = 32/64 (50%)
Frame = +3
Query: 396 LNQVIPETPEPTPEAVSNPAVSAPAPPKTRTSTRNTSTSLNNIEDGKVESDSEDQSVKSI 455
L ++ E + T AV PA+ P P K++ S N S++ + + + S ++ +
Sbjct: 864 LRDILKEQGKKTSSAV--PAIPVPTPQKSQPSQANWSSASSQ----SISASSPSKAASPL 1025
Query: 456 PVNT 459
P+N+
Sbjct: 1026PINS 1037
>CA923192 similar to GP|20196906|gb| expressed protein {Arabidopsis
thaliana}, partial (16%)
Length = 762
Score = 29.3 bits (64), Expect = 5.8
Identities = 15/36 (41%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
Frame = +2
Query: 400 IPETPEPTPEAVSNPAVS-APAPPKTRTSTRNTSTS 434
IP T + + + PA++ APAP T TSTR++++S
Sbjct: 317 IPSTSTSSTSSSATPALALAPAPCSTSTSTRDSASS 424
>TC88705 similar to GP|10177834|dbj|BAB11263. gene_id:MCD7.9~unknown protein
{Arabidopsis thaliana}, partial (32%)
Length = 1685
Score = 28.9 bits (63), Expect = 7.6
Identities = 26/115 (22%), Positives = 51/115 (43%), Gaps = 9/115 (7%)
Frame = +1
Query: 293 EEGEVTSKDIKDLMEQANYTNKYLQTLGESQ----SHDDSSRKGKGKVKTEPSSSSDLDI 348
E + TSK D + + T KY + + + + H+D S K V++EP S D
Sbjct: 637 ETDDDTSKYDDDTGKYDDDTGKYDEDINDEEFQEDEHEDLSSSYKSDVESEPDLSDDPSW 816
Query: 349 PKGCQLEKPLFKHFQISN-----RSRHSAQTLRAKNESDKELLQKVVEQLQLLNQ 398
+ +++K ++ Q+ N ++ A +R + + L K+ ++ L Q
Sbjct: 817 LE--KIQKSVWNIIQVVNIFQTPVNQSDAARIRKEYDESSAKLSKIQSRISSLTQ 975
>TC87714 similar to PIR|S22697|S22697 extensin - Volvox carteri (fragment),
partial (4%)
Length = 810
Score = 28.9 bits (63), Expect = 7.6
Identities = 16/44 (36%), Positives = 18/44 (40%)
Frame = +1
Query: 396 LNQVIPETPEPTPEAVSNPAVSAPAPPKTRTSTRNTSTSLNNIE 439
LN +PE P P P P S P PPK T L +E
Sbjct: 487 LNVTVPEPPRPRPP----PRPSPPPPPKPEEVENGTKVCLVALE 606
>TC76369 similar to PIR|T52336|T52336 probable type 1 membrane protein
[imported] - Arabidopsis thaliana, partial (10%)
Length = 1571
Score = 28.9 bits (63), Expect = 7.6
Identities = 11/36 (30%), Positives = 22/36 (60%), Gaps = 1/36 (2%)
Frame = +1
Query: 5 KSYFLRTNCEKSGETTL-FLTDYDKANVIVPKTIQW 39
+S+ + C+K F+TDY K++ +V +++QW
Sbjct: 709 RSFVSHSKCQKGNSNAS*FITDYTKSS*VVNRSLQW 816
>TC89281 similar to GP|11994197|dbj|BAB01300.
emb|CAA18500.1~gene_id:MPN9.10~similar to unknown
protein {Arabidopsis thaliana}, partial (43%)
Length = 733
Score = 28.9 bits (63), Expect = 7.6
Identities = 22/105 (20%), Positives = 47/105 (43%), Gaps = 3/105 (2%)
Frame = +1
Query: 376 RAKNESDKELLQK--VVEQ-LQLLNQVIPETPEPTPEAVSNPAVSAPAPPKTRTSTRNTS 432
R ++D+E L++ + EQ L+L N + P+ P+ + V ++ S
Sbjct: 211 RKTQKADREKLRRDRLNEQFLELGNILDPDRPKNDKATILGDTVQLLKDLSSQVSKLKDE 390
Query: 433 TSLNNIEDGKVESDSEDQSVKSIPVNTVIHNSRNQWRKETKLYYP 477
++ N E ++ + D + + + I N NQ++ + + YP
Sbjct: 391 YTMLNEESRELSQEKNDLREEKASLKSDIENLNNQYQLQLRTMYP 525
>TC84713
Length = 677
Score = 28.5 bits (62), Expect = 9.9
Identities = 22/81 (27%), Positives = 35/81 (43%), Gaps = 1/81 (1%)
Frame = -1
Query: 359 FKHFQISNRSRHSAQTLRAKNESDKELLQKVVEQLQLLNQVIPETPEPTPEAVSNPAVSA 418
F+H + +N +T ++ S L L+ P+T P V+ A+
Sbjct: 482 FEHHESNNARAFKPETRTTRDPS-----------LLLIFSKTPQTNSTPPFFVAIAALPF 336
Query: 419 PA-PPKTRTSTRNTSTSLNNI 438
P+ PP TS NTST +N+
Sbjct: 335 PSSPPHNSTSLSNTSTISSNV 273
>BQ751262 similar to GP|6715100|gb|A versicolorin B synthase {Aspergillus
parasiticus}, partial (11%)
Length = 710
Score = 28.5 bits (62), Expect = 9.9
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +3
Query: 81 RRNSFSIPRSIRTSSDFQSAMSRRSFSMSRRNQPESSARPS 121
RR S P + RTS S RRS S + + P SS+ P+
Sbjct: 492 RRRSSPKPSAARTSRSTPSPSPRRSSSTATKRPPASSSPPT 614
>TC77527 similar to GP|9293881|dbj|BAB01784.1 hydroxyproline-rich
glycoprotein {Arabidopsis thaliana}, partial (76%)
Length = 1829
Score = 28.5 bits (62), Expect = 9.9
Identities = 22/90 (24%), Positives = 43/90 (47%), Gaps = 4/90 (4%)
Frame = +2
Query: 28 KANVIVPKTIQWSDITLPDKW--SLEKAAPAQPVETPEFREIIQH--QSGQVDLIFDRRN 83
K+ ++V T++ + +KW SLE+ + V+TP+ +QH G VD N
Sbjct: 695 KSRMLVTPTVKKLAKDVAEKWKVSLEERGGVENVKTPDVHTFLQHLVTFGIVD-----SN 859
Query: 84 SFSIPRSIRTSSDFQSAMSRRSFSMSRRNQ 113
+ R + +S ++ M + + S+ +Q
Sbjct: 860 DLGLYRKLVIASAWRKHMPKLALSLGLTDQ 949
>TC79504 weakly similar to GP|21734794|gb|AAM76972.1 reduced vernalization
response 1 {Arabidopsis thaliana}, partial (26%)
Length = 695
Score = 28.5 bits (62), Expect = 9.9
Identities = 14/48 (29%), Positives = 26/48 (54%)
Frame = +1
Query: 295 GEVTSKDIKDLMEQANYTNKYLQTLGESQSHDDSSRKGKGKVKTEPSS 342
G + KD M+Q + ++Q E++ +SRK +G K++PS+
Sbjct: 517 GALQGKDSTGCMDQLIPADNHIQKRQENKDTKKTSRKKRGSRKSDPSA 660
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.131 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,845,728
Number of Sequences: 36976
Number of extensions: 315237
Number of successful extensions: 1783
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1672
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1774
length of query: 751
length of database: 9,014,727
effective HSP length: 103
effective length of query: 648
effective length of database: 5,206,199
effective search space: 3373616952
effective search space used: 3373616952
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0220.1