BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0218.25
(334 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87061 similar to GP|21593620|gb|AAM65587.1 unknown {Arabidopsi... 616 e-177
TC83859 39 0.003
TC80913 similar to PIR|T05616|T05616 hypothetical protein F20D10... 39 0.004
TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2... 36 0.018
TC82627 weakly similar to PIR|T51843|T51843 RING-H2 finger prote... 36 0.018
TC78659 similar to PIR|G96810|G96810 unknown protein T11I11.9 [i... 33 0.12
TC85903 similar to PIR|B84530|B84530 probable RING zinc finger p... 32 0.26
TC91547 weakly similar to GP|6815070|dbj|BAA90357.1 EST AU070319... 31 0.58
TC83086 similar to GP|11994153|dbj|BAB01182. protein kinase-like... 31 0.58
TC93889 31 0.58
TC79126 similar to PIR|H96703|H96703 probable RING zinc finger p... 31 0.76
TC79788 weakly similar to GP|21554189|gb|AAM63268.1 putative leu... 31 0.76
BQ751226 similar to GP|18376350|em probable aldehyde dehydrogena... 30 1.3
TC83595 similar to PIR|T47938|T47938 RING finger protein - Arabi... 30 1.3
TC93287 30 1.7
TC84165 similar to GP|21539497|gb|AAM53301.1 putative protein {A... 30 1.7
TC89515 weakly similar to GP|11994144|dbj|BAB01165. contains sim... 29 2.2
TC86742 similar to PIR|T01541|T01541 hypothetical protein A_IG00... 29 2.2
TC77426 similar to GP|15624006|dbj|BAB68060. putative PGPD14 pro... 29 2.2
TC77425 similar to GP|15624006|dbj|BAB68060. putative PGPD14 pro... 29 2.2
>TC87061 similar to GP|21593620|gb|AAM65587.1 unknown {Arabidopsis
thaliana}, partial (88%)
Length = 1594
Score = 616 bits (1589), Expect = e-177
Identities = 296/336 (88%), Positives = 313/336 (93%), Gaps = 2/336 (0%)
Frame = +1
Query: 1 MVVCKCRKATKLYCFVHKVPVCGECICFPEHQICVIRTYSEWVIDGEYDWPPKCCQCQAV 60
MVVCKCRKATKLYCFVHKVPVCGECICFPEHQICVIRTYSEWVIDGEYDWPPKCCQCQA+
Sbjct: 259 MVVCKCRKATKLYCFVHKVPVCGECICFPEHQICVIRTYSEWVIDGEYDWPPKCCQCQAI 438
Query: 61 LEEGDGSQTTRLGCLHVIHTNCLVSHIKSFPPHTAPAGYACPTCSTSIWPPKSVKDSGSR 120
LEEG GS+TTRLGCLHVIHTNCLVSHIKSFPPHTAPAGY CP+CSTSIWPPKSVKDSGSR
Sbjct: 439 LEEGGGSETTRLGCLHVIHTNCLVSHIKSFPPHTAPAGYVCPSCSTSIWPPKSVKDSGSR 618
Query: 121 LHSKLKEAIMQTGMEKNIFGNHPVSLSVTESRSPPPAFASEPLIGRENHGNSDSPATGSE 180
LHSKLKEAI+QTGMEKNIFGNHPVSLSVTESRSPPPAF+S+PLI REN G S S A GSE
Sbjct: 619 LHSKLKEAILQTGMEKNIFGNHPVSLSVTESRSPPPAFSSDPLISRENLGKSVS-ADGSE 795
Query: 181 PPKLSVTDIVEIEGANSAGNFVKGSSPVGP--ATRKGAFNVERQNSEISYYADDEDGNRK 238
KLSVTDIVEI+GANS GNF+K SSPVGP TRKG+ +V+RQNSEISYYADDED NRK
Sbjct: 796 LSKLSVTDIVEIDGANSGGNFMKSSSPVGPGATTRKGSIHVDRQNSEISYYADDEDANRK 975
Query: 239 KYTKRGPFYHKFLRALLPFWSSALPTLPVTAPPRKDASNATEGSEGRTRHQRSSRMDPRK 298
KYTKRGPF+HKFLRALLPFWSSALPTLPVTAP RKD SNATE SE RTRHQ+SSRMDPRK
Sbjct: 976 KYTKRGPFHHKFLRALLPFWSSALPTLPVTAPARKDGSNATETSEVRTRHQKSSRMDPRK 1155
Query: 299 ILLLIAIMACMATMGILYYRLVQRGPGEELPNEEQI 334
ILLLIAIMACMATMGILYYRLVQRGPG+EL N +++
Sbjct: 1156ILLLIAIMACMATMGILYYRLVQRGPGDELLNNDEL 1263
>TC83859
Length = 554
Score = 38.9 bits (89), Expect = 0.003
Identities = 22/66 (33%), Positives = 34/66 (51%)
Frame = +3
Query: 53 KCCQCQAVLEEGDGSQTTRLGCLHVIHTNCLVSHIKSFPPHTAPAGYACPTCSTSIWPPK 112
+C C +EEGD + + L C H+ H +CL + SF HT CP C S+ P +
Sbjct: 222 ECVVCLCKIEEGD--EISVLRCDHMYHKHCLDKWL-SFKNHT------CPLCRESLRPER 374
Query: 113 SVKDSG 118
++ + G
Sbjct: 375 AITEHG 392
>TC80913 similar to PIR|T05616|T05616 hypothetical protein F20D10.10 -
Arabidopsis thaliana, partial (7%)
Length = 995
Score = 38.5 bits (88), Expect = 0.004
Identities = 32/124 (25%), Positives = 51/124 (40%), Gaps = 4/124 (3%)
Frame = +2
Query: 54 CCQCQAVLEEGDGSQTTRLGCLHVIHTNCLVSHIKSFPPHTAPAGYACPTCSTS---IWP 110
C C ++ G+G C H+ H C+ +H+K+ T CP C T+ + P
Sbjct: 431 CGICTHSVKTGEGKAIFTAECSHIFHFPCIAAHVKNQQIIT------CPVCGTNWNDLQP 592
Query: 111 PKSVKDSGSRLHSKLKEAIMQTGMEKNIFGNHPVSLSVTESR-SPPPAFASEPLIGRENH 169
K+ +++ + SKL N + P+ S + SR S P E L EN
Sbjct: 593 EKTAENAKTTTLSKL----------PNYNDDEPLLSSASVSRFSTIPENEEEELEKEENE 742
Query: 170 GNSD 173
N +
Sbjct: 743 ENKE 754
>TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
[imported] - Arabidopsis thaliana, partial (69%)
Length = 1554
Score = 36.2 bits (82), Expect = 0.018
Identities = 24/98 (24%), Positives = 45/98 (45%)
Frame = +3
Query: 138 IFGNHPVSLSVTESRSPPPAFASEPLIGRENHGNSDSPATGSEPPKLSVTDIVEIEGANS 197
+F HP S + + S PP + +PATG EPP+L++ V+ + ++
Sbjct: 609 VFSAHPNSPPMAPASSSPP----------QRGDGEQTPATGEEPPQLTLVPPVQTDQVSA 758
Query: 198 AGNFVKGSSPVGPATRKGAFNVERQNSEISYYADDEDG 235
+G+ GS+ + ++N R ++ S + D G
Sbjct: 759 SGS---GSAALATDNLGLSYNSRRSPNQSSPSSQDRAG 863
>TC82627 weakly similar to PIR|T51843|T51843 RING-H2 finger protein RHA2b
[imported] - Arabidopsis thaliana, partial (44%)
Length = 716
Score = 36.2 bits (82), Expect = 0.018
Identities = 22/73 (30%), Positives = 32/73 (43%)
Frame = +3
Query: 53 KCCQCQAVLEEGDGSQTTRLGCLHVIHTNCLVSHIKSFPPHTAPAGYACPTCSTSIWPPK 112
+C CQ+ E GD +L C HV H +CL F + CP C S+ +
Sbjct: 417 RCVFCQSNFENGD--HVRKLPCRHVFHRHCLNGWFHRF-------NFNCPLCRCSLHSDE 569
Query: 113 SVKDSGSRLHSKL 125
V + R+ S+L
Sbjct: 570 RVALTERRVGSQL 608
>TC78659 similar to PIR|G96810|G96810 unknown protein T11I11.9 [imported] -
Arabidopsis thaliana, partial (85%)
Length = 1289
Score = 33.5 bits (75), Expect = 0.12
Identities = 27/97 (27%), Positives = 43/97 (43%), Gaps = 12/97 (12%)
Frame = +1
Query: 153 SPPPAFASEPLIGR-----------ENHGNSDSPATGSEPPKLSVTDIVEIEGANSAGNF 201
+PPP P+ R E+HG+ D AT EP + I+ + A +GN
Sbjct: 697 APPPEIKPRPITPRILELKGSIGLGESHGDGDQDATTGEPVLKTAKKILNQKFAELSGNN 876
Query: 202 V-KGSSPVGPATRKGAFNVERQNSEISYYADDEDGNR 237
+ KG SP A + + R+ S + +AD + +R
Sbjct: 877 IFKGDSPPLAAEKSLSGAKLREISGSNIFADGKAESR 987
>TC85903 similar to PIR|B84530|B84530 probable RING zinc finger protein
[imported] - Arabidopsis thaliana, partial (26%)
Length = 2572
Score = 32.3 bits (72), Expect = 0.26
Identities = 21/71 (29%), Positives = 27/71 (37%)
Frame = +3
Query: 37 RTYSEWVIDGEYDWPPKCCQCQAVLEEGDGSQTTRLGCLHVIHTNCLVSHIKSFPPHTAP 96
R Y + + D P CC CQ E DG L C H H+NC+ +
Sbjct: 2094 RFYMSLMTESSSDLEP-CCICQE--EYVDGQNLGLLDCGHEFHSNCITQWLMQ------- 2243
Query: 97 AGYACPTCSTS 107
CP C T+
Sbjct: 2244 -KNLCPICKTT 2273
>TC91547 weakly similar to GP|6815070|dbj|BAA90357.1 EST AU070319(S10788)
corresponds to a region of the predicted gene.~Similar
to RING-H2 finger, partial (24%)
Length = 601
Score = 31.2 bits (69), Expect = 0.58
Identities = 17/61 (27%), Positives = 25/61 (40%)
Frame = +1
Query: 54 CCQCQAVLEEGDGSQTTRLGCLHVIHTNCLVSHIKSFPPHTAPAGYACPTCSTSIWPPKS 113
C C + EEG+ + RL C H H +CL ++ + CP C + P
Sbjct: 313 CSVCLSEFEEGE--KVRRLKCKHTFHKDCLDKWLQDY-------FATCPLCREQVLPDNV 465
Query: 114 V 114
V
Sbjct: 466 V 468
>TC83086 similar to GP|11994153|dbj|BAB01182. protein kinase-like protein
{Arabidopsis thaliana}, partial (16%)
Length = 768
Score = 31.2 bits (69), Expect = 0.58
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +1
Query: 213 RKGAFNVERQNSEISYYADDEDGNRKKYTKR 243
RKGAF + + ++S++A DED KK KR
Sbjct: 190 RKGAFTGQHYDQDLSFHATDEDPVTKKTIKR 282
>TC93889
Length = 433
Score = 31.2 bits (69), Expect = 0.58
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +2
Query: 317 YRLVQRGPGEELPNEEQI 334
YRLVQRGPG+EL N +++
Sbjct: 2 YRLVQRGPGDELLNNDEL 55
>TC79126 similar to PIR|H96703|H96703 probable RING zinc finger protein
T23K23.8 [imported] - Arabidopsis thaliana, partial (81%)
Length = 1397
Score = 30.8 bits (68), Expect = 0.76
Identities = 17/56 (30%), Positives = 24/56 (42%)
Frame = +3
Query: 53 KCCQCQAVLEEGDGSQTTRLGCLHVIHTNCLVSHIKSFPPHTAPAGYACPTCSTSI 108
+CC C E DG + L C H H++C+V +K CP C +I
Sbjct: 1101 ECCICLCPYE--DGVELHTLPCNHHFHSSCIVKWLK--------MNATCPLCKYNI 1238
>TC79788 weakly similar to GP|21554189|gb|AAM63268.1 putative leucine-rich
repeat disease resistance protein {Arabidopsis
thaliana}, partial (52%)
Length = 1484
Score = 30.8 bits (68), Expect = 0.76
Identities = 37/129 (28%), Positives = 53/129 (40%), Gaps = 16/129 (12%)
Frame = +3
Query: 98 GYACPTCSTSI----WPPKSVKDSGSRLHSKLKEAIMQTGMEKNIFGNHPVSLSVTE--- 150
G+ C T ST I P S S + L SKL + I + N FG+ P S+S
Sbjct: 291 GFTCTTDSTHINQITLDPASYSGSLTPLISKLTQLITLDLSDNNFFGSIPSSISSLSNLK 470
Query: 151 ---------SRSPPPAFASEPLIGRENHGNSDSPATGSEPPKLSVTDIVEIEGANSAGNF 201
S PP+ S L E+ S + TGS P S+ ++ + + + N
Sbjct: 471 TLTLRSNSFSGPIPPSIIS--LKSLESLDLSHNSLTGSLPN--SLNSLINLHRIDLSFNK 638
Query: 202 VKGSSPVGP 210
+ GS P P
Sbjct: 639 LAGSIPKLP 665
>BQ751226 similar to GP|18376350|em probable aldehyde dehydrogenase
{Neurospora crassa}, partial (39%)
Length = 807
Score = 30.0 bits (66), Expect = 1.3
Identities = 26/80 (32%), Positives = 32/80 (39%), Gaps = 5/80 (6%)
Frame = +2
Query: 88 KSFPPHTAPAGYACPTCSTSIWPPKSVKDSGSRLHSKLKEA-----IMQTGMEKNIFGNH 142
+S PP T P STS PP + S L A T + + +
Sbjct: 230 RSLPPSTRPPR----RMSTSPSPPPARPSRASGTRPPLTSAPSCC*SSPTSPRRTLTCSP 397
Query: 143 PVSLSVTESRSPPPAFASEP 162
P+SLS TES SP P S P
Sbjct: 398 PLSLSTTESPSPWPRVTSVP 457
>TC83595 similar to PIR|T47938|T47938 RING finger protein - Arabidopsis
thaliana, partial (64%)
Length = 611
Score = 30.0 bits (66), Expect = 1.3
Identities = 25/95 (26%), Positives = 31/95 (32%), Gaps = 2/95 (2%)
Frame = +2
Query: 29 PEHQICVIRTYSEWVIDGEYDWPPKCCQCQAVLEEGDGSQTTRL--GCLHVIHTNCLVSH 86
P +IR + GE + C C L E + R C H+ H NC+
Sbjct: 206 PSVSALLIREFLPATKYGELTDTEEGCCCAVCLFEFSEEEEIRCLKNCRHIFHRNCVDRW 385
Query: 87 IKSFPPHTAPAGYACPTCSTSIWPPKSVKDSGSRL 121
I CP C T P V D RL
Sbjct: 386 IDLDQK-------TCPLCRTGFVPDDMVDDYNQRL 469
>TC93287
Length = 444
Score = 29.6 bits (65), Expect = 1.7
Identities = 22/71 (30%), Positives = 30/71 (41%), Gaps = 15/71 (21%)
Frame = +3
Query: 141 NHPVSLSVTESRSPPPAFASE-------PLIGRENHGNSDSPATGSEPPKLSVTDIV--- 190
N P S + R P PAFA+ P +GR H + + + S PP L + I
Sbjct: 117 NLPSSFLLLFPRRPHPAFAAYKLGSQKLPRVGRPVHDHHQTHTSSSSPPLLHPSPIAPSE 296
Query: 191 -----EIEGAN 196
E+EG N
Sbjct: 297 SRVLDELEGGN 329
>TC84165 similar to GP|21539497|gb|AAM53301.1 putative protein {Arabidopsis
thaliana}, partial (16%)
Length = 792
Score = 29.6 bits (65), Expect = 1.7
Identities = 13/51 (25%), Positives = 20/51 (38%)
Frame = +3
Query: 54 CCQCQAVLEEGDGSQTTRLGCLHVIHTNCLVSHIKSFPPHTAPAGYACPTC 104
C C ++ G+G C H H +C+ S++K CP C
Sbjct: 60 CAICLNTMKPGNGHAIFTAECSHSFHFHCITSNVKH-------GNQICPVC 191
>TC89515 weakly similar to GP|11994144|dbj|BAB01165. contains similarity to
nodulin~gene_id:K10D20.11 {Arabidopsis thaliana},
partial (43%)
Length = 854
Score = 29.3 bits (64), Expect = 2.2
Identities = 24/95 (25%), Positives = 39/95 (40%), Gaps = 2/95 (2%)
Frame = +2
Query: 117 SGSRLHSKLKEAIMQTGMEKNIFGNHPVSLSVTESRSPP-PAFASEPLIGRENH-GNSDS 174
SG++ + E + + N+ + + T + +PP P +S P N G S
Sbjct: 386 SGNKDNCLKNEKVTVIVLSDRSNNNNSSNTNQTSNATPPSPQSSSPPSPAPSNQEGQSPP 565
Query: 175 PATGSEPPKLSVTDIVEIEGANSAGNFVKGSSPVG 209
P T PP + +D +A NFV + VG
Sbjct: 566 PDTNQTPPPTATSDHDHPPRNGAASNFVSLAGSVG 670
>TC86742 similar to PIR|T01541|T01541 hypothetical protein A_IG005I10.16 -
Arabidopsis thaliana, partial (19%)
Length = 2073
Score = 29.3 bits (64), Expect = 2.2
Identities = 15/42 (35%), Positives = 21/42 (49%)
Frame = +3
Query: 145 SLSVTESRSPPPAFASEPLIGRENHGNSDSPATGSEPPKLSV 186
+LS++ S SP P S P + S +PA S PP +V
Sbjct: 684 TLSLSSSSSPSPPSPSPPTSSHPSTTTSSTPAPPSPPPSTTV 809
>TC77426 similar to GP|15624006|dbj|BAB68060. putative PGPD14 protein
(pollen germination related protein) {Oryza sativa
(japonica cultivar-group), partial (58%)
Length = 1003
Score = 29.3 bits (64), Expect = 2.2
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = +2
Query: 72 LGCLHVIHTNCLVSHIKSFPPHTAPAGYACPTCSTSI 108
L C H IH +CL + F YACP CS S+
Sbjct: 380 LHCGHTIHKSCLNEMREHFQ-------YACPLCSKSV 469
>TC77425 similar to GP|15624006|dbj|BAB68060. putative PGPD14 protein
(pollen germination related protein) {Oryza sativa
(japonica cultivar-group), partial (68%)
Length = 1002
Score = 29.3 bits (64), Expect = 2.2
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = +3
Query: 72 LGCLHVIHTNCLVSHIKSFPPHTAPAGYACPTCSTSI 108
L C H IH +CL + F YACP CS S+
Sbjct: 792 LHCGHTIHKSCLNEMREHFQ-------YACPLCSKSV 881
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.135 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,189,952
Number of Sequences: 36976
Number of extensions: 236398
Number of successful extensions: 1379
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 1368
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1376
length of query: 334
length of database: 9,014,727
effective HSP length: 97
effective length of query: 237
effective length of database: 5,428,055
effective search space: 1286449035
effective search space used: 1286449035
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0218.25