BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0218.16
(560 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW774387 weakly similar to PIR|T05210|T05 hypothetical protein F... 284 6e-77
BI310247 weakly similar to PIR|T05213|T05 hypothetical protein F... 174 4e-57
TC81274 similar to GP|23429644|gb|AAN10199.1 endoxylanase {Caric... 214 9e-56
TC87528 similar to PIR|D96617|D96617 probable xylan endohydrolas... 200 1e-51
BE317888 similar to PIR|T05693|T056 hypothetical protein F20M13.... 51 1e-06
AW689011 similar to PIR|D96617|D96 probable xylan endohydrolase ... 30 2.5
AW688565 similar to PIR|D96617|D96 probable xylan endohydrolase ... 30 3.2
BQ141880 weakly similar to GP|6523547|emb| hydroxyproline-rich g... 28 7.1
TC76904 homologue to GP|4325041|gb|AAD17230.1| FtsH-like protein... 28 9.3
>AW774387 weakly similar to PIR|T05210|T05 hypothetical protein F17I5.10 -
Arabidopsis thaliana (fragment), partial (19%)
Length = 658
Score = 284 bits (727), Expect = 6e-77
Identities = 137/199 (68%), Positives = 153/199 (76%)
Frame = -2
Query: 362 FLNEFNTIEYSGDVVANPANYIRKIREIQQFPGTKGIPLGIGLQCHFASGNPNLAYMRSG 421
F+NE+NTIEYSGD V +P NY++K+ EI Q I IGLQ HFASG PNLAYMRSG
Sbjct: 657 FMNEYNTIEYSGDKVVSPPNYLKKLEEIMQSGEATEILFAIGLQGHFASGQPNLAYMRSG 478
Query: 422 LDILGATGYPIWLTETSIDPQPNQEEYFEWILREGYSHPAVQGIIMFMGPVQAGFQKAPL 481
LD LG G+PIWLTE S+DPQPNQ EYFE +LRE YSHPAV+GIIMF GP QAGF L
Sbjct: 477 LDFLGNIGFPIWLTEASLDPQPNQAEYFEEVLREAYSHPAVEGIIMFAGPAQAGFNSTLL 298
Query: 482 ADENFTNTPMGDVVDKLIREWGTGPHETMADSRGIVDISLHHGDYDVIVSHPQTHFSKTL 541
AD NF TP G VVD LI EWG+GP+ +ADSRGIVDISLHHGDYDV +HP T SK L
Sbjct: 297 ADTNFQTTPTGQVVDNLILEWGSGPYTAIADSRGIVDISLHHGDYDVTFTHPLTQNSKKL 118
Query: 542 NLSVRKEFSQQTIHVKMHA 560
N+SVRK FSQ+ IHVKMHA
Sbjct: 117 NISVRKGFSQENIHVKMHA 61
>BI310247 weakly similar to PIR|T05213|T05 hypothetical protein F17I5.40 -
Arabidopsis thaliana, partial (39%)
Length = 841
Score = 174 bits (440), Expect(2) = 4e-57
Identities = 89/195 (45%), Positives = 124/195 (62%), Gaps = 1/195 (0%)
Frame = +1
Query: 20 ISEPQREQYGGGIIVNPAFDHSLEGWTMVGNGAIEKRRSNEGNAFIVARNRTKPLDSFSQ 79
++ PQ+ QY GGII NP + L+GWT GN IE R S GN ++VA NR +P DS +Q
Sbjct: 94 LAHPQKPQYNGGIIKNPELNDGLQGWTAFGNARIEHRESL-GNKYVVANNRNQPHDSVAQ 270
Query: 80 KVQLKKGMIYTFSAWLQLSKGSETVSVVFKI-HGSELIRGGHVIAKHGCWTLLKGGLVAN 138
K+ L+ G+ Y+ SAW+Q+S+ + V+ + K G + GG + A+ CW++LKGGL A+
Sbjct: 271 KIYLQNGLHYSLSAWIQVSEANVLVTAMVKTTEGFKF--GGAIYAEPNCWSMLKGGLTAD 444
Query: 139 FTSPADILFESKNPTVEIWADSVSLQPFTKEEWRSHQEDSIERVRKRKVRFQVTHINETA 198
T A++ FES N +VEIW D++SLQPFTK++W SHQE +IE+ RK+ V Q
Sbjct: 445 ATEVAELYFESNNTSVEIWIDNISLQPFTKKQWTSHQEQNIEKTRKKNVVVQALDEQGRP 624
Query: 199 LEGATISIKQTRPHF 213
L A ISI R F
Sbjct: 625 LPNARISITMKRYWF 669
Score = 66.2 bits (160), Expect(2) = 4e-57
Identities = 30/55 (54%), Positives = 36/55 (64%)
Frame = +3
Query: 213 FPFGCGMNNNILTNSAYRKWFMSRFKHTTFTNQMKWYSTEKVQGQENYTIPDAML 267
F FG MN IL+N+AY WF SRF T F N+MKWY+ E QG++NY D ML
Sbjct: 669 FHFGSAMNKYILSNTAYHYWFASRFTGTAF*NEMKWYTNEYTQGRDNYFEADVML 833
>TC81274 similar to GP|23429644|gb|AAN10199.1 endoxylanase {Carica papaya},
partial (33%)
Length = 912
Score = 214 bits (544), Expect = 9e-56
Identities = 109/235 (46%), Positives = 151/235 (63%), Gaps = 2/235 (0%)
Frame = +2
Query: 321 LIAWDVVNENLHYNFYEQNFGENASAMYYSTAYHLDPNARMFLNEFNTIEYSGDVVANPA 380
LI WDV+NENLH +F+E G+N ++ ++ +++D N +F+N++NTIE S D ++ P
Sbjct: 5 LIGWDVINENLHSSFFESKLGQNFTSRMFNEVHNVDGNTTLFMNDYNTIEDSRDGLSTPP 184
Query: 381 NYIRKIREIQQFPGTKGIPLGIGLQCHFASGNPNLAYMRSGLDILGATGYPIWLTETSID 440
YI+KIREIQ + K +PLGIGL+ HF + PNL YMR+ LDIL ATG PIW+TE +
Sbjct: 185 KYIQKIREIQSW--NKQLPLGIGLESHFPNFPPNLPYMRASLDILAATGLPIWITELDVA 358
Query: 441 PQPNQEEYFEWILREGYSHPAVQGIIMFMGPVQAGFQKAPLADENFTNTPMGDVVDKLIR 500
QPNQ Y E +LRE +SHP ++GI+M+ G + L D NF N P GDVVDKLI
Sbjct: 359 SQPNQAGYLEQVLREAHSHPGIRGIVMWTAWSPQGCFRMCLTDNNFKNLPTGDVVDKLIY 538
Query: 501 EWGTGPHETMADSRGIVDISLHHGDYDVIVSH-PQTHFSKTLNLSV-RKEFSQQT 553
EWG D G + SL HGDY + +SH + +++ T L V K+ S++T
Sbjct: 539 EWGRKILSGTTDKNGFLKASLFHGDYKMEISHSAKKNYTFTHQLQVLSKDKSKKT 703
>TC87528 similar to PIR|D96617|D96617 probable xylan endohydrolase isoenzyme
F9K23.10 [imported] - Arabidopsis thaliana, partial
(67%)
Length = 2080
Score = 200 bits (509), Expect = 1e-51
Identities = 155/557 (27%), Positives = 259/557 (45%), Gaps = 37/557 (6%)
Frame = +1
Query: 24 QREQYGGGIIVNPAFDHSLEGWTMVGNGAIEKRRSNE-----------------GNAFIV 66
Q +G II N +GW +GN + + + +I+
Sbjct: 271 QNVAFGVNIIENSNLSDDTKGWFTLGNCPLTVKTGSPHILPPMARESLGPHGILSGRYIL 450
Query: 67 ARNRTKPLDSFSQKV--QLKKGMIYTFSAWLQL---SKGSETVSVVFKIHGSELIRGGHV 121
NRT+ +Q + +LK + Y SAW+++ S G + V+V ++ I GG
Sbjct: 451 VTNRTQTWMGPAQVITEKLKLFLTYQVSAWVRIGSSSNGPQNVNVALGAD-NQWINGGQT 627
Query: 122 IAKHGCWTLLKGGLVANFTSPADILFESKNPT--VEIWADSVSLQPFTKEEWRSHQEDSI 179
W + GG P I+ + P V++ + + P + + +
Sbjct: 628 EVSDDRWHEI-GGSFRIEKQPTKIMVYIQGPASGVDLMVAGLQIFPVDRHARFRYLKMQT 804
Query: 180 ERVRKRKVRFQVTHINETALEGATISIKQTRPHFPFGCGMNNNILTNSAYRKWFMSRFKH 239
+++RKR V + ++ ++ + ++QT+ +FP G ++ + + N + + + F
Sbjct: 805 DKIRKRDVVLKFAGLDSSSYLNTMVQVRQTQNNFPIGTCISRSNIDNEDFVNFLVKHFNW 984
Query: 240 TTFTNQMKWYSTEKVQGQENYTIPDAMLALANDNGISVRGHTILWDDRNFQPEWVKSLSP 299
F N++KWY TE QG NY D +L L I RGH I W+ +WVKSL+
Sbjct: 985 AVFANELKWYWTEPQQGNLNYKDADDLLTLCQKYKIQTRGHCIFWEVDGTVQQWVKSLNK 1164
Query: 300 QDLREAARKRINSVVSRYKGDLIAWDVVNENLHYNFYEQNFGENASAMYYSTAYHLDPNA 359
DL A + R+ S+++RYKG +DV NE LH +FY+ G++ A + TA LD +A
Sbjct: 1165NDLMTAVQNRLTSLLTRYKGKFSHYDVNNEMLHGSFYQDRLGKDIRANMFKTANQLDLSA 1344
Query: 360 RMFLNEFNTIEYSGDVVANPANYIRKIREIQQFPGTKGIPL-GIGLQCHFASGNPNLAYM 418
+F+N+++ IE D + P YI I ++Q+ +G P+ GIG+Q H S P +
Sbjct: 1345TLFVNDYH-IEDGCDTRSCPNKYIEHILDLQE----QGAPVGGIGIQGHIDS--PVGPVV 1503
Query: 419 RSGLDILGATGYPIWLTETSIDPQPNQEEY-----FEWILREGYSHPAVQGIIMFMGPVQ 473
S LD LG G PIW TE + + EY E +LRE +HPAV+GI+++
Sbjct: 1504CSSLDKLGILGLPIWFTELDVS---SMNEYVRGDDLEVMLREAMAHPAVEGIMLW----- 1659
Query: 474 AGFQKAPLADEN-FTNTPMGDVVD------KLIREWGTGPHETMADSRGIVDISLHHGDY 526
GF + ++ +N GD+ + L +EW + H + + +G + +G Y
Sbjct: 1660-GFWELFMSRDNAHLVNAEGDINEAGKRFLALKQEWLSHSHGHV-NEQGQFNFRGFYGTY 1833
Query: 527 DVIVSHPQTHFSKTLNL 543
+V + P SKT L
Sbjct: 1834NVEIVTPSKKISKTFVL 1884
>BE317888 similar to PIR|T05693|T056 hypothetical protein F20M13.210 -
Arabidopsis thaliana, partial (13%)
Length = 205
Score = 50.8 bits (120), Expect = 1e-06
Identities = 21/59 (35%), Positives = 32/59 (53%)
Frame = +1
Query: 264 DAMLALANDNGISVRGHTILWDDRNFQPEWVKSLSPQDLREAARKRINSVVSRYKGDLI 322
D M+ N I RGH I W+D + P WV +L+ L+ A RI ++++YK + I
Sbjct: 25 DQMMQFVRANKIIARGHNIFWEDPKYNPAWVLNLTGTQLKSAVNSRIQRLMNQYKAEFI 201
>AW689011 similar to PIR|D96617|D96 probable xylan endohydrolase isoenzyme
F9K23.10 [imported] - Arabidopsis thaliana, partial
(12%)
Length = 591
Score = 30.0 bits (66), Expect = 2.5
Identities = 26/105 (24%), Positives = 46/105 (43%), Gaps = 11/105 (10%)
Frame = +2
Query: 32 IIVNPAFDHSLEGWTMVGNG---AIEKRRSN------EGNAFIVARNRTKPLDSFSQKV- 81
II+NP F+ L WT G G A+ + +N G F A RT+ + Q++
Sbjct: 203 IILNPEFEDGLNNWT--GRGCKIAVHESMANGKILPKSGKFFASATERTQSWNGIQQEIT 376
Query: 82 -QLKKGMIYTFSAWLQLSKGSETVSVVFKIHGSELIRGGHVIAKH 125
++++ + Y +A ++ + T S V + R I +H
Sbjct: 377 GRVQRKLAYEVTALXRIFGNNVTTSDVRATLYVQKTRSSRAIYRH 511
>AW688565 similar to PIR|D96617|D96 probable xylan endohydrolase isoenzyme
F9K23.10 [imported] - Arabidopsis thaliana, partial
(11%)
Length = 614
Score = 29.6 bits (65), Expect = 3.2
Identities = 23/87 (26%), Positives = 42/87 (47%), Gaps = 11/87 (12%)
Frame = +2
Query: 32 IIVNPAFDHSLEGWTMVGNG---AIEKRRSN------EGNAFIVARNRTKPLDSFSQKV- 81
II+NP F+ L WT G G A+ + +N G F A RT+ + Q++
Sbjct: 209 IILNPEFEDGLNNWT--GRGCKIAVHESMANGKILPKSGKFFASATERTQSWNGIQQEIT 382
Query: 82 -QLKKGMIYTFSAWLQLSKGSETVSVV 107
++++ + Y +A +++ + T S V
Sbjct: 383 GRVQRKLAYEVTALVRIFGNNVTTSDV 463
>BQ141880 weakly similar to GP|6523547|emb| hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (11%)
Length = 1233
Score = 28.5 bits (62), Expect = 7.1
Identities = 12/31 (38%), Positives = 19/31 (60%)
Frame = -2
Query: 107 VFKIHGSELIRGGHVIAKHGCWTLLKGGLVA 137
++ +H ++R V+AKHG WT L L+A
Sbjct: 1193 LYTLHIISILR*HRVVAKHGLWTGLASMLIA 1101
>TC76904 homologue to GP|4325041|gb|AAD17230.1| FtsH-like protein Pftf
precursor {Nicotiana tabacum}, partial (91%)
Length = 2667
Score = 28.1 bits (61), Expect = 9.3
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +2
Query: 516 IVDISLHHGDYDVIVSHPQTHF 537
+VDIS HHGD+ + Q HF
Sbjct: 215 MVDISFHHGDFHP*IIRHQNHF 280
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.135 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,107,964
Number of Sequences: 36976
Number of extensions: 248007
Number of successful extensions: 1152
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1147
length of query: 560
length of database: 9,014,727
effective HSP length: 101
effective length of query: 459
effective length of database: 5,280,151
effective search space: 2423589309
effective search space used: 2423589309
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0218.16