BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0212.11
(511 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AJ502495 weakly similar to GP|18071369|g putative gag-pol polypr... 57 2e-08
AW689768 weakly similar to GP|10177485|d polyprotein {Arabidopsi... 57 2e-08
TC89912 weakly similar to PIR|B84512|B84512 probable retroelemen... 54 2e-07
BG586293 weakly similar to PIR|E84473|E84 probable retroelement ... 49 5e-06
BG587156 similar to PIR|G85055|G8 probable polyprotein [imported... 48 1e-05
TC85125 weakly similar to SP|P10978|POLX_TOBAC Retrovirus-relate... 44 2e-04
CB893805 similar to GP|10177935|d copia-type polyprotein {Arabid... 43 3e-04
BG586159 weakly similar to PIR|T47841|T4 hypothetical protein T2... 35 0.002
BF650113 weakly similar to GP|4753889|emb| Tpv2-1c {Phaseolus vu... 31 0.99
TC79362 WD-repeat cell cycle regulatory protein 30 2.9
BE941052 weakly similar to PIR|B85188|B85 retrotransposon like p... 29 4.9
TC86982 similar to GP|10946337|gb|AAG24863.1 CONSTANS-like prote... 28 6.4
>AJ502495 weakly similar to GP|18071369|g putative gag-pol polyprotein {Oryza
sativa}, partial (9%)
Length = 542
Score = 56.6 bits (135), Expect = 2e-08
Identities = 34/105 (32%), Positives = 54/105 (51%), Gaps = 3/105 (2%)
Frame = +2
Query: 361 DNDFKKSASGYLIKFVGGVVALKSRLHRCIALSTFEAEFIDITEACKEFL*LKKLFQD*V 420
D + +KS SGY G ++ S+ +A ST EAE+I T + + L+++ + V
Sbjct: 17 DTETRKSTSGYAFHLGTGAISWSSKKQPVVAFSTAEAEYIASTSCATQTVWLRRILE--V 190
Query: 421 L---SRTNTCCFVIVKVRFILRKSPTFHSTSKHIDVRCHWIRDAL 462
+ T T + K L K+P FH SKHID++ H IR+ +
Sbjct: 191 MHHEQNTPTKIYCDNKSAIALSKNPVFHGRSKHIDIQFHKIRELI 325
>AW689768 weakly similar to GP|10177485|d polyprotein {Arabidopsis thaliana},
partial (9%)
Length = 675
Score = 56.6 bits (135), Expect = 2e-08
Identities = 33/101 (32%), Positives = 54/101 (52%), Gaps = 4/101 (3%)
Frame = +1
Query: 7 MDVMKNEMKSWHDNCSFHLVKLPKGKKALENKWICMVKLESNSTSLRYKAILMVEDFRHG 66
+ MK E K+ DN ++ LV LP KKA+ KW+ VK + + ++KA L+ + F
Sbjct: 100 LQAMKTEYKALIDNKTWDLVPLPPHKKAIGCKWVYRVKENPDGSVNKFKARLVAKGFSQT 279
Query: 67 KSVDSNE----MVKMSFIEIVLSLVATLDLEVKQIDVKAAF 103
D E ++K I ++L++ T E++QID+ AF
Sbjct: 280 LGCDYTETFSPVIKPVTIRLILTIAITYKWEIQQIDINNAF 402
>TC89912 weakly similar to PIR|B84512|B84512 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(10%)
Length = 814
Score = 53.5 bits (127), Expect = 2e-07
Identities = 29/105 (27%), Positives = 52/105 (48%)
Frame = +1
Query: 358 YVVDNDFKKSASGYLIKFVGGVVALKSRLHRCIALSTFEAEFIDITEACKEFL*LKKLFQ 417
Y + D +KS SG++ G ++ K+ + LST +AE+I E K+ + LK +
Sbjct: 112 YAGNVDTRKSLSGFVFTLYGTTISWKANQQSVVTLSTTQAEYIAFVEGVKDAIWLKGMIG 291
Query: 418 D*VLSRTNTCCFVIVKVRFILRKSPTFHSTSKHIDVRCHWIRDAL 462
+ +++ + L +H +KHID+R H+IRD +
Sbjct: 292 ELGITQEYVKIHCDSQSAIHLANHQVYHERTKHIDIRLHFIRDMI 426
>BG586293 weakly similar to PIR|E84473|E84 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(7%)
Length = 763
Score = 48.9 bits (115), Expect = 5e-06
Identities = 30/97 (30%), Positives = 53/97 (53%), Gaps = 4/97 (4%)
Frame = +2
Query: 17 WHDNCSFHLVKLPKGKKALENKWICMVKLESNSTSLRYKAILMVEDFRHGKSVDSNE--- 73
++ + LVK P G K + +WI +K + T ++YKA L+ + + + +D +E
Sbjct: 41 YYQKQTLKLVKKPTGVKPIGLRWIYKIKRNEDGTLIKYKARLVAKGYVKQQGIDFDEVFA 220
Query: 74 -MVKMSFIEIVLSLVATLDLEVKQIDVKAAFPSW*FV 109
+V++ I ++L+L AT + IDVK AF + FV
Sbjct: 221 PVVRIETI*LLLALAATNGC*IHHIDVKIAFLNGHFV 331
>BG587156 similar to PIR|G85055|G8 probable polyprotein [imported] -
Arabidopsis thaliana, partial (17%)
Length = 618
Score = 47.8 bits (112), Expect = 1e-05
Identities = 31/88 (35%), Positives = 48/88 (54%), Gaps = 4/88 (4%)
Frame = -1
Query: 20 NCSFHLVKLPKGKKALENKWICMVKLESNSTSLRYKAILMVEDFRHGKSVDSNE----MV 75
N +++ +LPKGKKA+ ++WI +K +++ + R K L+ F D E +
Sbjct: 480 NDTWYESELPKGKKAVSSRWIFTIKYKADGSIERKKTRLVARGFTLTYGEDYIETFAPVA 301
Query: 76 KMSFIEIVLSLVATLDLEVKQIDVKAAF 103
K+ I IVLSL L + Q+DVK AF
Sbjct: 300 KLHTIRIVLSLAVNLGWGLWQMDVKNAF 217
>TC85125 weakly similar to SP|P10978|POLX_TOBAC Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
(EC 3.4.23.-);, partial (7%)
Length = 705
Score = 43.5 bits (101), Expect = 2e-04
Identities = 27/80 (33%), Positives = 44/80 (54%), Gaps = 1/80 (1%)
Frame = +1
Query: 361 DNDFKKSASGYLIKFVGGVVALKSRLHRCIALSTFEAEFI-DITEACKEFL*LKKLFQD* 419
D+D +KS +GY+ GG V+ S+L +ALST EAE++ + ++ K +++
Sbjct: 106 DHDKRKSTTGYVFTLAGGAVSWLSKLQTVVALSTTEAEYMAAYLKHARKLFGCKG*WRNS 285
Query: 420 VLSRTNTCCFVIVKVRFILR 439
SR C V V+V IL+
Sbjct: 286 GTSRNKLLCIVTVRVPCILQ 345
Score = 35.8 bits (81), Expect = 0.040
Identities = 14/61 (22%), Positives = 35/61 (56%)
Frame = +3
Query: 402 ITEACKEFL*LKKLFQD*VLSRTNTCCFVIVKVRFILRKSPTFHSTSKHIDVRCHWIRDA 461
+ +ACKE + +++L ++ + + + + ++P FHS +KHI ++ H++R+
Sbjct: 231 LPQACKEAIWMQRLMEELGHKQEQITVYCDSQSALHIARNPAFHSRTKHIGIQYHFVREV 410
Query: 462 L 462
+
Sbjct: 411 V 413
>CB893805 similar to GP|10177935|d copia-type polyprotein {Arabidopsis
thaliana}, partial (14%)
Length = 778
Score = 42.7 bits (99), Expect = 3e-04
Identities = 26/85 (30%), Positives = 41/85 (47%), Gaps = 4/85 (4%)
Frame = +3
Query: 10 MKNEMKSWHDNCSFHLVKLPKGKKALENKWICMVKLESNSTSLRYKAILMVEDFRHGKSV 69
M NEM++ N ++ L L G K + KWI KL N +YKA L+ + + V
Sbjct: 90 MNNEMEATERNNTWELTDLRSGAKTIGLKWIFKTKLNENGEIEKYKARLVAKGYSQQYGV 269
Query: 70 DSNE----MVKMSFIEIVLSLVATL 90
D E + + I +V++L A +
Sbjct: 270 DYTEVFAPVARWDTIRMVIALAAQI 344
>BG586159 weakly similar to PIR|T47841|T4 hypothetical protein T2O9.150 -
Arabidopsis thaliana, partial (11%)
Length = 732
Score = 35.4 bits (80), Expect(2) = 0.002
Identities = 18/43 (41%), Positives = 24/43 (54%)
Frame = +1
Query: 358 YVVDNDFKKSASGYLIKFVGGVVALKSRLHRCIALSTFEAEFI 400
Y D D +KS SGY+ G V+ S+ + LST +AEFI
Sbjct: 385 YAGDLDDRKSTSGYVFMLSSGAVSWSSKKQPVVTLSTTKAEFI 513
Score = 23.5 bits (49), Expect(2) = 0.002
Identities = 14/46 (30%), Positives = 21/46 (45%)
Frame = +3
Query: 288 CW*FDVCNGVHKTRYSACCW*NQEMSNKSG*RALECCEIDFEVSLW 333
CW DV + HKT + C N ++ S * C + ++S W
Sbjct: 180 CWLSDVLSS-HKT*SNVCVKSN*QIHELSN*VTHACSQKSTQISQW 314
>BF650113 weakly similar to GP|4753889|emb| Tpv2-1c {Phaseolus vulgaris},
partial (13%)
Length = 494
Score = 31.2 bits (69), Expect = 0.99
Identities = 17/54 (31%), Positives = 31/54 (56%)
Frame = +1
Query: 365 KKSASGYLIKFVGGVVALKSRLHRCIALSTFEAEFIDITEACKEFL*LKKLFQD 418
++S SGY+ KF ++ ++ ALS++EAE+I T A + L L + ++
Sbjct: 316 RRSTSGYVFKFNDAAISWCTKKQPITALSSYEAEYIAGTFATFQALWLDSVIKE 477
>TC79362 WD-repeat cell cycle regulatory protein
Length = 1800
Score = 29.6 bits (65), Expect = 2.9
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = +2
Query: 284 LCICCW*FDVCNGVHKTRYSACCW 307
LC+ CW + TRYS+CCW
Sbjct: 794 LCLFCW-------LGSTRYSSCCW 844
>BE941052 weakly similar to PIR|B85188|B85 retrotransposon like protein
[imported] - Arabidopsis thaliana, partial (4%)
Length = 480
Score = 28.9 bits (63), Expect = 4.9
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = +2
Query: 438 LRKSPTFHSTSKHIDVRCHWIRD 460
L +P +HS KHI + H++RD
Sbjct: 53 LTHNPVYHSRMKHISIDIHFVRD 121
>TC86982 similar to GP|10946337|gb|AAG24863.1 CONSTANS-like protein {Ipomoea
nil}, partial (54%)
Length = 1643
Score = 28.5 bits (62), Expect = 6.4
Identities = 15/54 (27%), Positives = 32/54 (58%)
Frame = +2
Query: 321 LECCEIDFEVSLWF*LLIV*GFVLEVISLPMGLD*F*YVVDNDFKKSASGYLIK 374
++ C ++ +S+W L+++ G V++ +SLP+ L +V+N+ K +L K
Sbjct: 632 MDICLVEKLMSIWTLLIVILGGVMKTLSLPIILIIMMSIVNNNNNKIIMVFLKK 793
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.367 0.164 0.593
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,748,498
Number of Sequences: 36976
Number of extensions: 215657
Number of successful extensions: 3437
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1415
Number of HSP's successfully gapped in prelim test: 155
Number of HSP's that attempted gapping in prelim test: 1958
Number of HSP's gapped (non-prelim): 1674
length of query: 511
length of database: 9,014,727
effective HSP length: 100
effective length of query: 411
effective length of database: 5,317,127
effective search space: 2185339197
effective search space used: 2185339197
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.7 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0212.11