BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0206b.5
(199 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF636730 49 1e-06
TC89338 48 3e-06
TC91682 40 6e-04
BQ149799 37 0.007
AJ548089 37 0.007
CA921077 34 0.043
BI270951 homologue to GP|20906098|gb Cobalamin biosynthesis prot... 34 0.043
TC87858 similar to GP|9757903|dbj|BAB08350.1 gene_id:MMN10.5~unk... 31 0.36
AW685577 weakly similar to GP|10177905|d gb|AAF07790.1~gene_id:M... 30 0.62
TC90978 weakly similar to GP|9279697|dbj|BAB01254.1 centromere p... 29 1.4
TC78826 similar to PIR|G96607|G96607 probable galactinol synthas... 27 5.2
BF646796 similar to GP|20466195|gb putative adenosine deaminase ... 27 5.2
TC83511 similar to GP|18252961|gb|AAL62407.1 unknown protein {Ar... 27 6.8
BG449544 similar to GP|10177133|db contains similarity to nucleo... 26 8.9
TC88228 similar to GP|13605916|gb|AAK32943.1 AT4g00830/A_TM018A1... 26 8.9
>BF636730
Length = 637
Score = 49.3 bits (116), Expect = 1e-06
Identities = 27/81 (33%), Positives = 42/81 (51%), Gaps = 8/81 (9%)
Frame = +1
Query: 8 KSWIE---MPRNTMAYEE---GVKRFIDFAFERSSIE--GKIICPCPRCEFRKRQTRDEV 59
+ W++ P N+ +E GV F++FA + + GK+ CPC C+ RK + D+V
Sbjct: 301 RKWMDNRMRPDNSRVTDEFLAGVCEFVEFACAQDDFKKVGKLRCPCKICKCRKP*SVDDV 480
Query: 60 YDHLICKQLPKGYTFWFDHGE 80
HL K + Y +W HGE
Sbjct: 481 MKHLCMKGFKEDYYYWSSHGE 543
>TC89338
Length = 1088
Score = 47.8 bits (112), Expect = 3e-06
Identities = 28/87 (32%), Positives = 45/87 (51%), Gaps = 2/87 (2%)
Frame = +3
Query: 1 LYLKAMDKSWIEMPRNTMAYEEGVKRFIDFAFER-SSIEGKIICPCPRCEFRK-RQTRDE 58
L + + K W++ R + Y+ GV F+ FA + + EG CPC C R + +++E
Sbjct: 201 LLIGKIAKRWMKAYRLSAEYDNGVTEFLQFAEKNLPNTEGLFPCPCVSCGNRDPKLSKEE 380
Query: 59 VYDHLICKQLPKGYTFWFDHGETMNNS 85
+ HL+ + + YT HGETM S
Sbjct: 381 IKGHLVSVGICQNYTQ*IWHGETMMTS 461
Score = 45.8 bits (107), Expect = 1e-05
Identities = 23/68 (33%), Positives = 37/68 (53%), Gaps = 2/68 (2%)
Frame = +3
Query: 20 YEEGVKRFIDFAFER-SSIEGKIICPCPRC-EFRKRQTRDEVYDHLICKQLPKGYTFWFD 77
Y+ G+ F+ FA + + EG CPC C + + +++E+ HL+ + + YT W
Sbjct: 501 YDNGMTEFLQFAEKNLPNTEGLFPCPCISCGNWDPKLSKEEIRGHLVSVGICQNYTEWIW 680
Query: 78 HGETMNNS 85
HGETM S
Sbjct: 681 HGETMMTS 704
>TC91682
Length = 1093
Score = 40.0 bits (92), Expect = 6e-04
Identities = 16/33 (48%), Positives = 25/33 (75%)
Frame = +2
Query: 148 LARDGEQPLYEGCTKYSKLSFLVKLYHIKTLCG 180
L RD + PLY+GC K+++LS ++KLY++K G
Sbjct: 5 LVRDSKIPLYDGCAKFTRLSTVLKLYNLKARNG 103
>BQ149799
Length = 437
Score = 36.6 bits (83), Expect = 0.007
Identities = 18/59 (30%), Positives = 33/59 (55%), Gaps = 2/59 (3%)
Frame = +1
Query: 6 MDKSWIEMPRNTMAYEEGVKRFIDFAFER-SSIEGKIICPCPRCEFR-KRQTRDEVYDH 62
MD+ W++ R ++ Y+ GV F+ FA + + EG CPC C R + +++++ H
Sbjct: 259 MDRRWMKAYRLSVEYDNGVTEFLQFAEKNLPNTEGLFPCPCVSCGNRDPKLSKEKIRGH 435
>AJ548089
Length = 560
Score = 36.6 bits (83), Expect = 0.007
Identities = 17/49 (34%), Positives = 27/49 (54%), Gaps = 1/49 (2%)
Frame = +1
Query: 38 EGKIICPCPRC-EFRKRQTRDEVYDHLICKQLPKGYTFWFDHGETMNNS 85
EG CPC C + + +++E+ HL+ + + YT W +GETM S
Sbjct: 226 EGLFPCPCISCGNWDPKLSKEEIRGHLVSVGICQNYTEWIWYGETMMTS 372
>CA921077
Length = 438
Score = 33.9 bits (76), Expect = 0.043
Identities = 16/35 (45%), Positives = 21/35 (59%), Gaps = 1/35 (2%)
Frame = -2
Query: 29 DFAFERSSIEGK-IICPCPRCEFRKRQTRDEVYDH 62
D AF + +E + I+CPC C +TRD VYDH
Sbjct: 437 DIAFTKGMVEEEEILCPCAVCCNDSWETRDVVYDH 333
>BI270951 homologue to GP|20906098|gb Cobalamin biosynthesis protein
{Methanosarcina mazei Goe1}, partial (0%)
Length = 432
Score = 33.9 bits (76), Expect = 0.043
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +1
Query: 8 KSWIEMPRNTMAYEEGVKRFIDFAFERSSIE 38
K W+ +P ++ +Y++GV F+D AF + +E
Sbjct: 337 KDWMALPPHSQSYKDGVNYFLDIAFTKGMVE 429
>TC87858 similar to GP|9757903|dbj|BAB08350.1 gene_id:MMN10.5~unknown protein
{Arabidopsis thaliana}, partial (34%)
Length = 2037
Score = 30.8 bits (68), Expect = 0.36
Identities = 14/58 (24%), Positives = 26/58 (44%)
Frame = +1
Query: 42 ICPCPRCEFRKRQTRDEVYDHLICKQLPKGYTFWFDHGETMNNSPQNVPNSVEQTMVD 99
+C C C + K E H CK +F++G+T+ Q + ++ E ++ D
Sbjct: 1258 LCGCQSCNYAKGLNAFEFEKHAGCKSKHPNNHIYFENGKTIYQIVQELRSTPESSLFD 1431
>AW685577 weakly similar to GP|10177905|d
gb|AAF07790.1~gene_id:MNJ7.28~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (13%)
Length = 652
Score = 30.0 bits (66), Expect = 0.62
Identities = 14/52 (26%), Positives = 23/52 (43%), Gaps = 1/52 (1%)
Frame = +3
Query: 2 YLKAMDKSWIEMPRNTMAYEEGVKRFI-DFAFERSSIEGKIICPCPRCEFRK 52
Y + K W R T +Y + + ++ F R S GK+ C C C ++
Sbjct: 474 YFECSSKGWWYNSRTTSSYVKFCRPYVRKTMFRRQSETGKVCCACSSCNNKR 629
>TC90978 weakly similar to GP|9279697|dbj|BAB01254.1 centromere protein
{Arabidopsis thaliana}, partial (5%)
Length = 1132
Score = 28.9 bits (63), Expect = 1.4
Identities = 17/59 (28%), Positives = 26/59 (43%)
Frame = +1
Query: 64 ICKQLPKGYTFWFDHGETMNNSPQNVPNSVEQTMVDEDPIQNMINGAFGVDMHHGEESN 122
+C Q K Y GE +N P+ +E + M++G FG D H GE+ +
Sbjct: 502 VCNQEQKDYVIETCLGENIN------PSVIENNL--------MLDGVFGFDRHEGEDXS 636
>TC78826 similar to PIR|G96607|G96607 probable galactinol synthase F25P12.95
[imported] - Arabidopsis thaliana, partial (83%)
Length = 1238
Score = 26.9 bits (58), Expect = 5.2
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -3
Query: 47 RCEFRKRQTRDEVYDHLICKQLPKGYTFWF 76
+C K+ + D + +H + Q PKG FWF
Sbjct: 252 QCNLVKQGSYDVLQEHQVR*QQPKGTWFWF 163
>BF646796 similar to GP|20466195|gb putative adenosine deaminase {Arabidopsis
thaliana}, partial (46%)
Length = 616
Score = 26.9 bits (58), Expect = 5.2
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = -2
Query: 82 MNNSPQNVPNSVEQTMVDEDPIQNMINGAFGVDMHH 117
++ P SVE M DP++ N FG+D+HH
Sbjct: 132 LSPKPLASSRSVESLM---DPLR*ACNSTFGIDIHH 34
>TC83511 similar to GP|18252961|gb|AAL62407.1 unknown protein {Arabidopsis
thaliana}, complete
Length = 1014
Score = 26.6 bits (57), Expect = 6.8
Identities = 13/46 (28%), Positives = 23/46 (49%), Gaps = 1/46 (2%)
Frame = +3
Query: 144 DFYDLARDGEQPLYEGCTK-YSKLSFLVKLYHIKTLCGVTDKAMTM 188
+FY+L D + P GC + S++ L + HI + C ++ M
Sbjct: 687 EFYNLESDSDLPKAPGCEREESEIEELYGVLHIPSSCSTLNRCCCM 824
>BG449544 similar to GP|10177133|db contains similarity to nucleolar
protein~gene_id:K2A18.26 {Arabidopsis thaliana}, partial
(33%)
Length = 685
Score = 26.2 bits (56), Expect = 8.9
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = -1
Query: 113 VDMHHGEESNEDVPDGEGVIPDATQERHETRDFY 146
+D H E++ + E V+P AT+ RH + Y
Sbjct: 559 LDSHESEQNPRGIT--ENVVPSATKSRHRSPSLY 464
>TC88228 similar to GP|13605916|gb|AAK32943.1 AT4g00830/A_TM018A10_14
{Arabidopsis thaliana}, partial (67%)
Length = 1712
Score = 26.2 bits (56), Expect = 8.9
Identities = 19/82 (23%), Positives = 35/82 (42%)
Frame = -1
Query: 60 YDHLICKQLPKGYTFWFDHGETMNNSPQNVPNSVEQTMVDEDPIQNMINGAFGVDMHHGE 119
+DHL+ P + D P ++ +V++ + IQ + + +H
Sbjct: 1451 FDHLVKPLEPSAFLLALD--------PYHISLAVDKQQQQQHHIQPLQSHKDFQRSYH-- 1302
Query: 120 ESNEDVPDGEGVIPDATQERHE 141
N+DV DG + D T+ RH+
Sbjct: 1301 --NQDVEDG*DGVQDCTRSRHQ 1242
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.138 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,737,264
Number of Sequences: 36976
Number of extensions: 94949
Number of successful extensions: 404
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 403
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 404
length of query: 199
length of database: 9,014,727
effective HSP length: 91
effective length of query: 108
effective length of database: 5,649,911
effective search space: 610190388
effective search space used: 610190388
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0206b.5