BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0206b.3
(304 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89161 similar to GP|21536687|gb|AAM61019.1 unknown {Arabidopsi... 276 5e-75
AW981475 similar to GP|10140699|gb| unknown protein {Oryza sativ... 55 3e-08
BG587583 similar to GP|17979131|g unknown protein {Arabidopsis t... 55 3e-08
TC92839 similar to GP|12654435|gb|AAH01043.1 Unknown (protein fo... 52 4e-07
TC80016 similar to GP|14335160|gb|AAK59860.1 AT3g52120/F4F15_230... 47 9e-06
TC81055 similar to PIR|T46013|T46013 hypothetical protein T10K17... 47 1e-05
TC84943 similar to PIR|E86306|E86306 Similar to tuftelin-interac... 40 0.001
TC88322 similar to GP|15810008|gb|AAL06931.1 At1g63980/F22C12_9 ... 39 0.002
TC88509 similar to PIR|T01826|T01826 microfibril-associated prot... 39 0.002
AW687009 similar to GP|15982781|gb| AT3g09850/F8A24_10 {Arabidop... 38 0.004
BI265310 weakly similar to GP|14043850|gb| Similar to splicing f... 36 0.016
BF647277 similar to GP|15810008|gb| At1g63980/F22C12_9 {Arabidop... 34 0.080
TC92282 similar to GP|2367591|gb|AAB69694.1| putative transcript... 31 0.52
TC87637 similar to PIR|T02462|T02462 probable AT-hook DNA-bindin... 31 0.52
TC89509 similar to GP|16604561|gb|AAL24082.1 unknown protein {Ar... 31 0.67
TC92431 homologue to PIR|H88465|H88465 protein B0244.3 [imported... 30 1.5
AJ503279 similar to GP|15293301|gb| unknown protein {Arabidopsis... 29 2.6
BQ137224 similar to GP|1934808|emb| unknown {Bacillus subtilis},... 28 3.3
TC84539 28 4.4
BG452363 similar to PIR|T07950|T07 peptidylprolyl isomerase (EC ... 28 4.4
>TC89161 similar to GP|21536687|gb|AAM61019.1 unknown {Arabidopsis
thaliana}, partial (26%)
Length = 937
Score = 276 bits (707), Expect = 5e-75
Identities = 140/168 (83%), Positives = 153/168 (90%), Gaps = 2/168 (1%)
Frame = +2
Query: 139 KREVLAEREQKIQTEVKEIRKVFYCDLCNKQYKLAMEFEAHLSSYDHNHRKRFKQMKEMH 198
K +VLAEREQKIQTEVKEIRKVF+C+LCNKQYKLAMEFEAHLSSYDHNHRKRFK+MKEMH
Sbjct: 200 KIQVLAEREQKIQTEVKEIRKVFFCELCNKQYKLAMEFEAHLSSYDHNHRKRFKEMKEMH 379
Query: 199 GGSSRDDRQKREQQRQEREIAKFAQIADAQKQQRLQLLQESGSE--PVSSETRTATPLTD 256
G SSRDDRQKREQQRQERE+AKFAQIADAQKQQRLQL QESGS VSSE++TAT LTD
Sbjct: 380 GSSSRDDRQKREQQRQERELAKFAQIADAQKQQRLQLQQESGSAAVSVSSESKTATALTD 559
Query: 257 QEQRNALKFGFSSKGSASKNVIGPKRQMVAKKQNIPVSSIFNNDSDEE 304
QEQRN LKFGFS+KG+ASK+ G K+Q V KKQN+PV+SIF NDSDEE
Sbjct: 560 QEQRNTLKFGFSAKGTASKSTFGVKKQNVPKKQNLPVASIFGNDSDEE 703
>AW981475 similar to GP|10140699|gb| unknown protein {Oryza sativa}, partial
(73%)
Length = 634
Score = 55.5 bits (132), Expect = 3e-08
Identities = 36/110 (32%), Positives = 61/110 (54%), Gaps = 6/110 (5%)
Frame = +3
Query: 59 SLDTQLTSSNIGFKLLQKMGWKGK-GLGKDEQGIIEPIKSGMRDPRLGLGKQEEDDFFTA 117
S T + SSNIGF+LL+K GWK + GLG EQG +EP+++ +++ + GLG
Sbjct: 87 SATTTINSSNIGFQLLKKHGWKERTGLGISEQGRLEPVETRVKNNKRGLGADIVKKKVVK 266
Query: 118 EENIQRKKLDVELEETEENVRKREVLAEREQKIQ-----TEVKEIRKVFY 162
+ K D + E+ + +K + L++R +K+Q + KE + F+
Sbjct: 267 PGHGDSSKGDNKQEQLSQ--KKAKALSKRVRKMQEIEKKMQEKEFERAFF 410
>BG587583 similar to GP|17979131|g unknown protein {Arabidopsis thaliana},
partial (11%)
Length = 773
Score = 55.1 bits (131), Expect = 3e-08
Identities = 25/64 (39%), Positives = 42/64 (65%), Gaps = 1/64 (1%)
Frame = +2
Query: 49 NVDLENVEQASLDTQLTSSNIGFKLLQKMGWK-GKGLGKDEQGIIEPIKSGMRDPRLGLG 107
+V+L+ E + D + N+G ++L+ MGW+ G GLGKD G+IEP+++ + R GLG
Sbjct: 539 DVNLDTFEVITADKAIDEKNVGNRMLRNMGWQEGLGLGKDGSGMIEPVQTQAMENRAGLG 718
Query: 108 KQEE 111
Q++
Sbjct: 719 SQQK 730
>TC92839 similar to GP|12654435|gb|AAH01043.1 Unknown (protein for MGC:1134)
{Homo sapiens}, partial (28%)
Length = 692
Score = 51.6 bits (122), Expect = 4e-07
Identities = 33/74 (44%), Positives = 43/74 (57%), Gaps = 8/74 (10%)
Frame = +3
Query: 61 DTQLTSSNIGFKLLQKMGWK-GKGLGKDEQGIIEPI-KSGMRDPRLGLGK------QEED 112
+ +L SNIGFK+L K GW+ G GLGK +GI PI K+ R GLG+ +EED
Sbjct: 123 NNKLDQSNIGFKMLMKQGWQAGSGLGKSGEGIAAPINKADSRPENAGLGQTKPEGVEEED 302
Query: 113 DFFTAEENIQRKKL 126
D F I RK++
Sbjct: 303 DEF----EIYRKRM 332
>TC80016 similar to GP|14335160|gb|AAK59860.1 AT3g52120/F4F15_230 {Arabidopsis
thaliana}, partial (65%)
Length = 1543
Score = 47.0 bits (110), Expect = 9e-06
Identities = 23/58 (39%), Positives = 39/58 (66%), Gaps = 2/58 (3%)
Frame = +1
Query: 52 LENVEQASLDTQLTSSNIGFKLLQKMGWK-GKGLGKDEQGIIEPIKSG-MRDPRLGLG 107
++ ++A+ ++ + N+G KLL KMGWK G+GLG +GI +PI +G ++ LG+G
Sbjct: 1216 MKAAKEAAERAKIQADNVGHKLLSKMGWKEGEGLGGSRKGIADPIMAGSVKKDNLGVG 1389
>TC81055 similar to PIR|T46013|T46013 hypothetical protein T10K17.120 -
Arabidopsis thaliana, partial (54%)
Length = 697
Score = 46.6 bits (109), Expect = 1e-05
Identities = 30/108 (27%), Positives = 56/108 (51%), Gaps = 3/108 (2%)
Frame = +3
Query: 48 ENVDLENVEQ--ASLDTQLTSSNIGFKLLQKMGW-KGKGLGKDEQGIIEPIKSGMRDPRL 104
E ++ EQ A ++ + SNIGFKLL++MG+ G LGK G EP+ +R R
Sbjct: 255 ERKQIQEDEQTLAKVEAPIPQSNIGFKLLKQMGYTPGSALGKQGSGRAEPVGIEIRRSRA 434
Query: 105 GLGKQEEDDFFTAEENIQRKKLDVELEETEENVRKREVLAEREQKIQT 152
G+G ++ +E +R+++ ++ + E E + ++ + Q+
Sbjct: 435 GIGLEDPH-----KEKKKREEITIDRKRKNEQALMEEFGSRQKSRWQS 563
>TC84943 similar to PIR|E86306|E86306 Similar to tuftelin-interacting
protein [imported] - Arabidopsis thaliana, partial (25%)
Length = 1066
Score = 39.7 bits (91), Expect = 0.001
Identities = 16/26 (61%), Positives = 23/26 (87%)
Frame = +1
Query: 75 QKMGWKGKGLGKDEQGIIEPIKSGMR 100
+KMG+KG GLGK+EQGI+ PI++ +R
Sbjct: 1 EKMGYKGGGLGKNEQGILNPIEAKLR 78
>TC88322 similar to GP|15810008|gb|AAL06931.1 At1g63980/F22C12_9
{Arabidopsis thaliana}, partial (54%)
Length = 1373
Score = 39.3 bits (90), Expect = 0.002
Identities = 26/80 (32%), Positives = 46/80 (57%), Gaps = 6/80 (7%)
Frame = +1
Query: 71 FKLLQKMGWK-GKGLGKDEQGIIEPIKSGMRDPRLGLGKQEEDD--FFTAEENIQRKKLD 127
F+L+++MGW+ G+GLGK++QGI ++ + G+G ++ + F T + + K+L
Sbjct: 139 FRLMKQMGWEEGEGLGKEKQGIKGHVRVTNKQDTTGIGLEKPNQWAFDTTQFDNILKRLK 318
Query: 128 VEL---EETEENVRKREVLA 144
V+ +TE N K E A
Sbjct: 319 VQAPVSNDTESNTVKVETEA 378
>TC88509 similar to PIR|T01826|T01826 microfibril-associated protein homolog
T15F16.8 - Arabidopsis thaliana, partial (83%)
Length = 1306
Score = 38.9 bits (89), Expect = 0.002
Identities = 29/100 (29%), Positives = 50/100 (50%), Gaps = 4/100 (4%)
Frame = +1
Query: 122 QRKKLDVELEETEENVRKREVLAEREQKIQTEVKEIRKVFYCDLCNKQYKLAMEFEAHLS 181
+R++L+ E EE E RKR + R + Q V+EIRK +++ + +E EA++
Sbjct: 640 ERERLEAE-EEALEEARKRRMEERRNETRQIVVEEIRK-------DQEIQKNIELEANID 795
Query: 182 SYDH----NHRKRFKQMKEMHGGSSRDDRQKREQQRQERE 217
D N + ++ K G + DR+ RE +E+E
Sbjct: 796 DVDTDDELNEAEEYEAWKVREIGRIKRDREDREALLKEKE 915
>AW687009 similar to GP|15982781|gb| AT3g09850/F8A24_10 {Arabidopsis
thaliana}, partial (6%)
Length = 185
Score = 38.1 bits (87), Expect = 0.004
Identities = 19/46 (41%), Positives = 31/46 (67%), Gaps = 1/46 (2%)
Frame = +1
Query: 63 QLTSSNIGFKLLQKMGW-KGKGLGKDEQGIIEPIKSGMRDPRLGLG 107
++ ++ G K++ KMG+ +G GLGK+ QG+ +PI+ R LGLG
Sbjct: 13 EVHTTGFGSKMMAKMGYTEGGGLGKNGQGMAQPIEVIQRPKSLGLG 150
>BI265310 weakly similar to GP|14043850|gb| Similar to splicing factor (45kD)
{Homo sapiens}, partial (5%)
Length = 312
Score = 36.2 bits (82), Expect = 0.016
Identities = 19/44 (43%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = +1
Query: 72 KLLQKMGWK-GKGLGKDEQGIIEPIKSGMRDPRLGLGKQEEDDF 114
K L+K GW GKGLGK E GI E +K ++ G+G +F
Sbjct: 100 KQLEKYGWTDGKGLGKHENGISEALKPKLKRSVAGVGHDAAAEF 231
>BF647277 similar to GP|15810008|gb| At1g63980/F22C12_9 {Arabidopsis
thaliana}, partial (9%)
Length = 671
Score = 33.9 bits (76), Expect = 0.080
Identities = 14/34 (41%), Positives = 25/34 (73%), Gaps = 1/34 (2%)
Frame = +3
Query: 59 SLDTQLTSSNIGFKLLQKMGWK-GKGLGKDEQGI 91
S+ + + F+L+++MGW+ G+GLGK++QGI
Sbjct: 96 SITVGVARKSAAFRLMKQMGWEEGEGLGKEKQGI 197
>TC92282 similar to GP|2367591|gb|AAB69694.1| putative transcriptional
regulator {Magnaporthe grisea}, partial (13%)
Length = 285
Score = 31.2 bits (69), Expect = 0.52
Identities = 20/72 (27%), Positives = 33/72 (45%), Gaps = 2/72 (2%)
Frame = +3
Query: 156 EIRKVFYCDL--CNKQYKLAMEFEAHLSSYDHNHRKRFKQMKEMHGGSSRDDRQKREQQR 213
EI + + C+ C K Y AH+ H ++ + KE+ +Q R Q+R
Sbjct: 15 EIERHYACNYPGCTKSYGTLNHLNAHVQMQKHGQKRHPSEFKELR-------KQWRRQKR 173
Query: 214 QEREIAKFAQIA 225
+E E K A++A
Sbjct: 174 EEEE-RKAAEVA 206
>TC87637 similar to PIR|T02462|T02462 probable AT-hook DNA-binding protein
[imported] - Arabidopsis thaliana, partial (53%)
Length = 1659
Score = 31.2 bits (69), Expect = 0.52
Identities = 21/81 (25%), Positives = 42/81 (50%), Gaps = 3/81 (3%)
Frame = -3
Query: 138 RKREVLAEREQKIQTEVKEIRKVFYCDLCNKQYKLAMEFEAHLS-SYDHNHRKRFKQMKE 196
R+RE ERE++ ++++K + + CD C+K + EF S + + ++K
Sbjct: 295 RERERERERERERESKLKRVF*IVNCD-CDKVVHILHEFVVEASVKVEKCSTVSYGELKR 119
Query: 197 MHGG--SSRDDRQKREQQRQE 215
GG S ++R++ +Q+ E
Sbjct: 118 DDGG*QKSTEEREREREQKSE 56
>TC89509 similar to GP|16604561|gb|AAL24082.1 unknown protein {Arabidopsis
thaliana}, partial (63%)
Length = 721
Score = 30.8 bits (68), Expect = 0.67
Identities = 34/135 (25%), Positives = 60/135 (44%), Gaps = 8/135 (5%)
Frame = +3
Query: 177 EAHLSSY----DHNHRKRFKQMKEMHGGSSRDDRQKREQQRQEREIAKFAQIADAQKQQR 232
+ H+ Y D+ +RKR ++ +E+H S + QK + +R AK AQ ++
Sbjct: 243 DRHIKEYGRPLDYENRKRKREAREVHNHSKK--AQKTIGHKGKRN-AKKNYAEKAQMKRT 413
Query: 233 LQLLQESGSEPVSSET--RTATP--LTDQEQRNALKFGFSSKGSASKNVIGPKRQMVAKK 288
L + +ES S + + A P L D+E +++ N I KR+ A K
Sbjct: 414 LAMHEESTSRRKADDNVQEGAVPAYLLDREN--------TTRAKILSNTIKQKRKEKAGK 569
Query: 289 QNIPVSSIFNNDSDE 303
++P+ + DE
Sbjct: 570 WDVPLPKVRPVAEDE 614
>TC92431 homologue to PIR|H88465|H88465 protein B0244.3 [imported] -
Caenorhabditis elegans, partial (73%)
Length = 357
Score = 29.6 bits (65), Expect = 1.5
Identities = 15/43 (34%), Positives = 25/43 (57%), Gaps = 5/43 (11%)
Frame = +2
Query: 180 LSSYDHNHRKRFKQM-----KEMHGGSSRDDRQKREQQRQERE 217
+SS+ H HRKR ++ +E R+ ++RE++R ERE
Sbjct: 83 ISSHTHTHRKRERERERERERERERERERERERERERERGERE 211
>AJ503279 similar to GP|15293301|gb| unknown protein {Arabidopsis thaliana},
partial (16%)
Length = 332
Score = 28.9 bits (63), Expect = 2.6
Identities = 15/53 (28%), Positives = 24/53 (44%)
Frame = +2
Query: 184 DHNHRKRFKQMKEMHGGSSRDDRQKREQQRQEREIAKFAQIADAQKQQRLQLL 236
+HNH K + +D+ E+ + E+AKF D K ++QLL
Sbjct: 83 EHNHIKTPNSNNTIT--KHKDEEDDEEEDNMDVELAKFPTAGDPHKMAKMQLL 235
>BQ137224 similar to GP|1934808|emb| unknown {Bacillus subtilis}, partial
(17%)
Length = 1046
Score = 28.5 bits (62), Expect = 3.3
Identities = 36/159 (22%), Positives = 57/159 (35%), Gaps = 16/159 (10%)
Frame = +3
Query: 121 IQRKKLDVELEETEENVRKREVLAEREQK-----IQTEVKEIRKVFYCDLCNKQYKLAME 175
++ + + E+EETE+ ++REV++ K +TE +E R+ CN
Sbjct: 522 VEERDREREVEETEDEEKRREVMSIHNTKQHRYVRRTEQREERRA----RCN-------H 668
Query: 176 FEAHLSSYDHNHRKRFKQMKEMHGGSSRDDRQKREQQRQEREIAK-----------FAQI 224
E H + R + + M SRD + REQ Q K + Q
Sbjct: 669 TETH--RLRRHERPQ*SRTTTMTARQSRDTQHMREQTAQREHRNKEASNET**TYMYVQT 842
Query: 225 ADAQKQQRLQLLQESGSEPVSSETRTATPLTDQEQRNAL 263
+ +G S ETR+ T N L
Sbjct: 843 TVQIDHVDITTRHIAGKRTASDETRSVYAATSYRHTNTL 959
>TC84539
Length = 521
Score = 28.1 bits (61), Expect = 4.4
Identities = 14/54 (25%), Positives = 27/54 (49%)
Frame = +2
Query: 226 DAQKQQRLQLLQESGSEPVSSETRTATPLTDQEQRNALKFGFSSKGSASKNVIG 279
D +R++LL++SGS +SE +++ + + GF+S N+ G
Sbjct: 11 DVPSLKRMELLEQSGSADKNSEQTRVVNVSEGSPGSVMANGFNSSQPPKLNING 172
>BG452363 similar to PIR|T07950|T07 peptidylprolyl isomerase (EC 5.2.1.8) 1 -
Chlamydomonas reinhardtii, partial (88%)
Length = 586
Score = 28.1 bits (61), Expect = 4.4
Identities = 14/40 (35%), Positives = 24/40 (60%)
Frame = +1
Query: 189 KRFKQMKEMHGGSSRDDRQKREQQRQEREIAKFAQIADAQ 228
+RF+Q + HG +DR R+QQRQ+ + + ++ AQ
Sbjct: 346 RRFRQRDQGHG-RCEEDRVLRQQQRQDLQAHRHCRLWPAQ 462
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.311 0.130 0.351
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,627,749
Number of Sequences: 36976
Number of extensions: 72381
Number of successful extensions: 475
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 463
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 470
length of query: 304
length of database: 9,014,727
effective HSP length: 96
effective length of query: 208
effective length of database: 5,465,031
effective search space: 1136726448
effective search space used: 1136726448
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0206b.3