BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0206a.3
(552 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG449711 similar to GP|8467955|dbj ESTs AU091298(C61241) C28476(... 276 1e-74
AA660714 similar to PIR|T48005|T48 hypothetical protein T17J13.9... 143 1e-34
TC88912 similar to GP|16152176|gb|AAL14994.1 NIFS-like protein C... 47 1e-05
TC84395 similar to GP|11385579|gb|AAG34872.1 In2-1 protein {Glyc... 35 0.075
BG455047 similar to GP|11385579|gb| In2-1 protein {Glycine max},... 35 0.075
BI265380 weakly similar to GP|17932898|em isoamylase {Triticum a... 33 0.37
TC91264 weakly similar to PIR|T27543|T27543 hypothetical protein... 32 0.49
TC78634 similar to PIR|T48567|T48567 ABA-responsive protein homo... 32 0.63
AW684530 similar to GP|15724197|gb At2g39050/T7F6.22 {Arabidopsi... 32 0.83
TC78057 weakly similar to PIR|T03962|T03962 r40g3 protein - rice... 32 0.83
BF644174 weakly similar to PIR|E96721|E967 hypothetical protein ... 31 1.1
AW695267 similar to GP|15450731|gb At2g40130/T7M7.2 {Arabidopsis... 31 1.1
TC76989 homologue to SP|O24310|EFTU_PEA Elongation factor Tu ch... 31 1.1
CA860027 similar to GP|313746|emb|C YBLO506 {Saccharomyces cerev... 31 1.4
TC82570 similar to PIR|T51853|T51853 RING-H2 finger protein RHF1... 31 1.4
TC81780 homologue to GP|20521948|dbj|BAB13408. KIAA1582 protein ... 31 1.4
TC88711 similar to PIR|T07614|T07614 homeobox-leucine zipper pro... 30 1.8
BQ137371 30 1.8
TC89833 similar to SP|P45458|MASY_SOYBN Malate synthase glyoxys... 30 2.4
>BG449711 similar to GP|8467955|dbj ESTs AU091298(C61241) C28476(C61241)
correspond to a region of the predicted gene.~Similar
to, partial (32%)
Length = 505
Score = 276 bits (706), Expect = 1e-74
Identities = 135/166 (81%), Positives = 153/166 (91%)
Frame = +1
Query: 192 SRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVK 251
SR+II++LVNA ++EISIVDNATTAAAIVLQ+TAW FREG F GDVV+MLHYAYGAVK
Sbjct: 7 SRSIIQNLVNAKDINEISIVDNATTAAAIVLQHTAWCFREGKFNKGDVVVMLHYAYGAVK 186
Query: 252 KSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPC 311
KSMEAYVTRAGG VIEVPLPFPV S+D+IV EFR ALE+GK GKK+RLAVIDHVTSMPC
Sbjct: 187 KSMEAYVTRAGGKVIEVPLPFPVSSNDEIVTEFRKALEKGKVDGKKIRLAVIDHVTSMPC 366
Query: 312 VVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSN 357
VVIPVKEL++ICREEGV++VFVDAAHSIGCTDVDM++IGAD+YT N
Sbjct: 367 VVIPVKELIQICREEGVEQVFVDAAHSIGCTDVDMQDIGADYYTXN 504
>AA660714 similar to PIR|T48005|T48 hypothetical protein T17J13.90 -
Arabidopsis thaliana, partial (42%)
Length = 622
Score = 143 bits (361), Expect = 1e-34
Identities = 73/126 (57%), Positives = 93/126 (72%), Gaps = 1/126 (0%)
Frame = +2
Query: 169 WQLKYLRQPDNFYFNHLKQGILRSRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWA 228
WQL++L+QPD+F+FN L+ GIL SR IIK+L+NAD ++EIS++DNATTAAAIVLQ
Sbjct: 5 WQLRFLQQPDDFFFNTLRNGILDSRKIIKNLINADDVEEISLIDNATTAAAIVLQQIGHH 184
Query: 229 FREGTFQPGDVVLMLHYAYGAVKKSMEAYVTRAGGTVIEVPLPFPVKSSDDIVR-EFRSA 287
F G F D V++ H AY AVKKS+EAYV GG+VIEV LPFPV S++ R R
Sbjct: 185 FSSGKFCRNDTVIIFHCAYQAVKKSIEAYVIPVGGSVIEVELPFPVNSNEGNYRGNLRKG 364
Query: 288 LERGKS 293
+ERGK+
Sbjct: 365 IERGKN 382
Score = 126 bits (316), Expect = 2e-29
Identities = 57/94 (60%), Positives = 74/94 (78%), Gaps = 1/94 (1%)
Frame = +3
Query: 279 DIVREF-RSALERGKSGGKKVRLAVIDHVTSMPCVVIPVKELVKICREEGVDRVFVDAAH 337
+I+ E L GK G +VRLA+IDH+TSMP VVIPV+EL+++C E VD+VFVD AH
Sbjct: 336 EIIAEI*EKGLNEGKINGGRVRLAIIDHITSMPSVVIPVRELIRVCXENEVDQVFVDGAH 515
Query: 338 SIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAFL 371
++G +VD+KEIGADFY SNL+KWFF PPS+AF+
Sbjct: 516 ALGSMEVDVKEIGADFYVSNLYKWFFSPPSVAFM 617
>TC88912 similar to GP|16152176|gb|AAL14994.1 NIFS-like protein CpNifsp
precursor {Arabidopsis thaliana}, partial (52%)
Length = 991
Score = 47.4 bits (111), Expect = 1e-05
Identities = 43/184 (23%), Positives = 80/184 (43%), Gaps = 1/184 (0%)
Frame = +2
Query: 192 SRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVK 251
+R + +NA EI NAT A +V A+++ + GD +++ + +
Sbjct: 479 ARRKVAAFINASDSREIVFTRNATEAINLV----AYSWGLSNLKQGDEIVLTVAEHHSAI 646
Query: 252 KSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPC 311
+ + G + V L D ++E S KK ++ + HV+++
Sbjct: 647 VPWQLVAQKVGAVLKFVNLNQDEIPDIDKLKEVLS---------KKTKIVAVHHVSNVLA 799
Query: 312 VVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFC-PPSIAF 370
V+P++++ G +V VDA S+ D++ + ADF ++ HK C P I F
Sbjct: 800 SVLPIRDIAHWAHGVGA-KVLVDACQSVPHMVGDVQNLDADFLVASSHK--MCGPTGIGF 970
Query: 371 LYSR 374
LY +
Sbjct: 971 LYGK 982
>TC84395 similar to GP|11385579|gb|AAG34872.1 In2-1 protein {Glycine max},
partial (54%)
Length = 692
Score = 35.0 bits (79), Expect = 0.075
Identities = 17/57 (29%), Positives = 30/57 (51%)
Frame = +2
Query: 88 IFTHHFLISSSMASTHNHNNNNNGCTTHIPKKPKLSPLSSSPFITPSEIQSEFSHHD 144
I HHF I + +S+ + NNN + +P++ SS PF++ + I +S H+
Sbjct: 35 IHCHHFGIMAISSSSSSLVTNNNSSLFSVSARPQILVTSSLPFMSTTSIHFHYSPHN 205
>BG455047 similar to GP|11385579|gb| In2-1 protein {Glycine max}, partial
(14%)
Length = 637
Score = 35.0 bits (79), Expect = 0.075
Identities = 17/57 (29%), Positives = 30/57 (51%)
Frame = +1
Query: 88 IFTHHFLISSSMASTHNHNNNNNGCTTHIPKKPKLSPLSSSPFITPSEIQSEFSHHD 144
I HHF I + +S+ + NNN + +P++ SS PF++ + I +S H+
Sbjct: 4 IHCHHFGIMAISSSSSSLVTNNNSSLFSVSARPQILVTSSLPFMSTTSIHFHYSPHN 174
>BI265380 weakly similar to GP|17932898|em isoamylase {Triticum aestivum},
partial (13%)
Length = 604
Score = 32.7 bits (73), Expect = 0.37
Identities = 19/39 (48%), Positives = 24/39 (60%)
Frame = +1
Query: 38 CSLSKVKTHPLVSVPLIIYSISFSPLTHSILSLCLSSSS 76
C+ K HP +VPL+ + S SPL HSI S LS+SS
Sbjct: 268 CNFPKATPHP--TVPLLKKTGSISPLIHSIHSQLLSASS 378
>TC91264 weakly similar to PIR|T27543|T27543 hypothetical protein ZC395.10 -
Caenorhabditis elegans, partial (24%)
Length = 767
Score = 32.3 bits (72), Expect = 0.49
Identities = 32/99 (32%), Positives = 45/99 (45%), Gaps = 1/99 (1%)
Frame = -3
Query: 58 ISFSPLTHSILSLCLSSSSSSSYVVVLSLVIFTHHFLISSSMASTHNHNNNNNGCTTHIP 117
ISFS L S SL LSS S V SL++ + ++S+ + + + +
Sbjct: 546 ISFSSLLISFSSLLLSSLISLDSSFVESLILVVSSTVSKPGISSSESSSAESEPSPIRLE 367
Query: 118 KKPKLSPLSSSPFITPSEIQSEFSHH-DPSVARINNGSF 155
K + P+ S P PS S S H + SV R NGSF
Sbjct: 366 K--SIPPIGSGP---PSSSSSSSSFHLEKSVLR--NGSF 271
>TC78634 similar to PIR|T48567|T48567 ABA-responsive protein homolog
T31B5.20 - Arabidopsis thaliana, partial (67%)
Length = 1208
Score = 32.0 bits (71), Expect = 0.63
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = +2
Query: 79 SYVVVLSLVIFTHHFLISSSMASTHNHNNNNNGCTTHIPKKPKLSPLSSSPFITPSEIQS 138
SY++ +L +HH L+S N+NNNNN P +PF +P+ S
Sbjct: 50 SYIIHSTLF*ISHHKLLSLPFTYNMNNNNNNNNQNQSSP--------DENPFASPTPPSS 205
Query: 139 EFS 141
S
Sbjct: 206 SSS 214
>AW684530 similar to GP|15724197|gb At2g39050/T7F6.22 {Arabidopsis thaliana},
partial (33%)
Length = 643
Score = 31.6 bits (70), Expect = 0.83
Identities = 23/90 (25%), Positives = 48/90 (52%), Gaps = 10/90 (11%)
Frame = +3
Query: 57 SISFSPLTHSILSLCLSSSSSSSYVVVLSLVIFT------HHFLISSSMAS----THNHN 106
SI+ P ++++ ++++++++ + L I+ HH +I++ +++ TH+H+
Sbjct: 30 SITTPPT*PTLITTVVTTTTTNNIIHHLVTTIYLPSTNHHHHHIINNHLSTLTHHTHHHH 209
Query: 107 NNNNGCTTHIPKKPKLSPLSSSPFITPSEI 136
NNN HI K K S + P +T S I
Sbjct: 210 NNN-----HINPKLKSSTPGTCPVMTISTI 284
>TC78057 weakly similar to PIR|T03962|T03962 r40g3 protein - rice, partial
(50%)
Length = 878
Score = 31.6 bits (70), Expect = 0.83
Identities = 23/90 (25%), Positives = 48/90 (52%), Gaps = 10/90 (11%)
Frame = +3
Query: 57 SISFSPLTHSILSLCLSSSSSSSYVVVLSLVIFT------HHFLISSSMAS----THNHN 106
SI+ P ++++ ++++++++ + L I+ HH +I++ +++ TH+H+
Sbjct: 3 SITTPPT*PTLITTVVTTTTTNNIIHHLVTTIYLPSTNHHHHHIINNHLSTLTHHTHHHH 182
Query: 107 NNNNGCTTHIPKKPKLSPLSSSPFITPSEI 136
NNN HI K K S + P +T S I
Sbjct: 183 NNN-----HINPKLKSSTPGTCPVMTISTI 257
>BF644174 weakly similar to PIR|E96721|E967 hypothetical protein T17F3.6
[imported] - Arabidopsis thaliana, partial (13%)
Length = 362
Score = 31.2 bits (69), Expect = 1.1
Identities = 35/120 (29%), Positives = 50/120 (41%), Gaps = 13/120 (10%)
Frame = +1
Query: 35 HVLCSLSKVKTHPLVSVPLIIYSISFSPLTHSILSLC------LSSSSSSSYVVV--LSL 86
H + + KTHP P S P S C L+ S+ S++ + L+
Sbjct: 19 HTCPTNNTTKTHPSPCPPFQ----STPPFPFSTTPGCGHPSFQLTCSTPHSFITINNLTF 186
Query: 87 VIFTHHFLISSSMASTHN---HNNNNNGC--TTHIPKKPKLSPLSSSPFITPSEIQSEFS 141
I ++ +S + S HN NNN C T+ IP KP LS++PF E S S
Sbjct: 187 SILSYKPNSTSIILSPHNPISQQNNNTKCPTTSSIPNKP--INLSNTPFTLSDETCSRLS 360
>AW695267 similar to GP|15450731|gb At2g40130/T7M7.2 {Arabidopsis thaliana},
partial (29%)
Length = 625
Score = 31.2 bits (69), Expect = 1.1
Identities = 34/122 (27%), Positives = 52/122 (41%), Gaps = 2/122 (1%)
Frame = +2
Query: 94 LISSSMASTHNHNNNNNGCTTHIPKKPKLSPLSSSPFITPSEIQSEFSHHDPSVARINNG 153
L SSS+ + N+ + + K LS S +PS + S H+P V
Sbjct: 263 LPSSSILRDACSRSRNSAYSPRLQFKALDLCLSVSLDRSPSSHNNVSSDHEPPV------ 424
Query: 154 SFGCCSASVTAAQHDWQLKYLRQPDNFYFNHLKQGILRSRTIIKDLVNAD--HLDEISIV 211
S S+ AA Q R PDNF+F H +Q + +T V + HL +S++
Sbjct: 425 -----SNSLMAAIKRSQANQRRHPDNFHFYHQQQQLQSQQTFSVSSVKVELQHL-VLSVL 586
Query: 212 DN 213
D+
Sbjct: 587 DD 592
>TC76989 homologue to SP|O24310|EFTU_PEA Elongation factor Tu chloroplast
precursor (EF-Tu). [Garden pea] {Pisum sativum}, partial
(92%)
Length = 1907
Score = 31.2 bits (69), Expect = 1.1
Identities = 27/72 (37%), Positives = 38/72 (52%)
Frame = +3
Query: 65 HSILSLCLSSSSSSSYVVVLSLVIFTHHFLISSSMASTHNHNNNNNGCTTHIPKKPKLSP 124
+S +++ +SSSSS+ + TH F SSS + +N TTH PK L+
Sbjct: 135 NSTMAISISSSSSTKLKLSSPYPPHTHTFTTSSSASI-------SNPSTTHRPKL-TLTH 290
Query: 125 LSSSPFITPSEI 136
LSSS F+ PS I
Sbjct: 291 LSSS-FLNPSTI 323
>CA860027 similar to GP|313746|emb|C YBLO506 {Saccharomyces cerevisiae},
partial (14%)
Length = 661
Score = 30.8 bits (68), Expect = 1.4
Identities = 21/64 (32%), Positives = 31/64 (47%)
Frame = -2
Query: 20 HLFGFFNFGFPCGWIHVLCSLSKVKTHPLVSVPLIIYSISFSPLTHSILSLCLSSSSSSS 79
+LF +F F C W H SK T L+SV Y ISF SI+ C +S ++
Sbjct: 528 YLFSLLSFPF-CSW*HTFIFDSKFSTRYLISV*CRYYKISF--FWCSIIGKC*TSKNTII 358
Query: 80 YVVV 83
+++
Sbjct: 357 EMII 346
>TC82570 similar to PIR|T51853|T51853 RING-H2 finger protein RHF1a
[imported] - Arabidopsis thaliana (fragment), partial
(23%)
Length = 738
Score = 30.8 bits (68), Expect = 1.4
Identities = 26/93 (27%), Positives = 38/93 (39%), Gaps = 6/93 (6%)
Frame = +2
Query: 85 SLVIFTHHFLISSSMASTHNHNNNNNGCTTHIPKKPKLSPLSSSPFITPSEIQS------ 138
S +FTHHFL S + + ++ ++ P +P SSS F S S
Sbjct: 50 SFFLFTHHFLSSHLTLTMATFTSFSSPSSSSSTPPPFSAPSSSSAFDDDSFEDSCSICLE 229
Query: 139 EFSHHDPSVARINNGSFGCCSASVTAAQHDWQL 171
FS HDPS +VT +H++ L
Sbjct: 230 SFSVHDPS--------------TVTCCKHEYHL 286
>TC81780 homologue to GP|20521948|dbj|BAB13408. KIAA1582 protein {Homo
sapiens}, partial (1%)
Length = 787
Score = 30.8 bits (68), Expect = 1.4
Identities = 19/67 (28%), Positives = 34/67 (50%)
Frame = +2
Query: 86 LVIFTHHFLISSSMASTHNHNNNNNGCTTHIPKKPKLSPLSSSPFITPSEIQSEFSHHDP 145
L+I T HFL+ + H+H++NN+ HI P + ++S S S +SHH
Sbjct: 20 LIITTTHFLL---LHYHHHHHHNNHHNHHHIKTTPLIQTQTTSNHHLNS--SSSYSHHSH 184
Query: 146 SVARINN 152
++ ++
Sbjct: 185 NITTFHS 205
>TC88711 similar to PIR|T07614|T07614 homeobox-leucine zipper protein
homolog h1 - soybean, partial (73%)
Length = 1238
Score = 30.4 bits (67), Expect = 1.8
Identities = 34/104 (32%), Positives = 47/104 (44%), Gaps = 4/104 (3%)
Frame = -1
Query: 31 CGWIHVLCSL---SKVKTHPLVSVPLII-YSISFSPLTHSILSLCLSSSSSSSYVVVLSL 86
C + VLCSL S++ L S+ + S S S L S+ S SSS + + L
Sbjct: 581 CLGLRVLCSLKVSSRIADWSLESLSFFLAVSASSSSLIFSLFSTS*SSSLKLLFPLTLET 402
Query: 87 VIFTHHFLISSSMASTHNHNNNNNGCTTHIPKKPKLSPLSSSPF 130
V+F SSS S H+ + N T+ + LSPL S F
Sbjct: 401 VLFGEETPASSS--SWHSIAVDGNRFTSIPRRYFPLSPLHVSKF 276
>BQ137371
Length = 999
Score = 30.4 bits (67), Expect = 1.8
Identities = 15/39 (38%), Positives = 24/39 (61%), Gaps = 2/39 (5%)
Frame = -2
Query: 81 VVVLSLVIFTHH--FLISSSMASTHNHNNNNNGCTTHIP 117
V+++S V+ H+ +LI+SS A T + CTTH+P
Sbjct: 992 VLIISDVVIVHYLYYLITSSHADTPTALRRSVTCTTHVP 876
>TC89833 similar to SP|P45458|MASY_SOYBN Malate synthase glyoxysomal (EC
4.1.3.2) (MS) (Fragment). [Soybean] {Glycine max},
partial (39%)
Length = 842
Score = 30.0 bits (66), Expect = 2.4
Identities = 27/93 (29%), Positives = 43/93 (46%), Gaps = 5/93 (5%)
Frame = -1
Query: 51 VPLIIYSISFSPLTHSILSLCLSSSS-----SSSYVVVLSLVIFTHHFLISSSMASTHNH 105
+P +++++ S + + L +C SSS SS ++VV+ L F L S
Sbjct: 305 IPTLVFNLKPS-IVRTPLGICRRSSSVSFAASSRFIVVI*LGAFPMLLLKISIQ------ 147
Query: 106 NNNNNGCTTHIPKKPKLSPLSSSPFITPSEIQS 138
+ GC H+P P + LS S F+T S S
Sbjct: 146 DGIRPGCAAHVPSCPAFTSLSLS-FLTNSSAAS 51
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.137 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,059,812
Number of Sequences: 36976
Number of extensions: 382917
Number of successful extensions: 3146
Number of sequences better than 10.0: 80
Number of HSP's better than 10.0 without gapping: 2914
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3068
length of query: 552
length of database: 9,014,727
effective HSP length: 101
effective length of query: 451
effective length of database: 5,280,151
effective search space: 2381348101
effective search space used: 2381348101
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0206a.3