BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0204.9
(716 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90706 similar to PIR|E84825|E84825 probable protein kinase [im... 417 e-117
TC80490 similar to PIR|E84825|E84825 probable protein kinase [im... 233 2e-61
BG646013 similar to GP|20260382|gb unknown protein {Arabidopsis ... 175 5e-44
TC80692 similar to GP|20260382|gb|AAM13089.1 unknown protein {Ar... 155 7e-38
TC91317 similar to PIR|C96761|C96761 hypothetical protein T9L24.... 154 1e-37
TC79563 similar to SP|P51567|AFC2_ARATH Protein kinase AFC2 (EC ... 131 8e-31
CA989663 similar to GP|14164406|db putative protein kinase AFC3 ... 127 1e-29
TC78357 similar to GP|15289849|dbj|BAB63546. putative mitogen-ac... 119 8e-29
BG587590 similar to PIR|T04125|T04 protein kinase PK12 (EC 2.7.1... 124 2e-28
TC76803 PIR|T09622|T09622 protein kinase MMK4 (EC 2.7.1.-) cold... 119 5e-27
TC79500 similar to GP|9294059|dbj|BAB02016.1 MAP kinase {Arabido... 117 1e-26
CA917722 similar to GP|14164406|db putative protein kinase AFC3 ... 117 2e-26
TC82909 homologue to GP|17064906|gb|AAL32607.1 Unknown protein {... 115 6e-26
TC87466 homologue to GP|10185114|emb|CAC08564. wound-induced GSK... 109 3e-24
TC77039 GP|313148|emb|CAA48472.1| protein kinase {Medicago sativ... 108 5e-24
TC83072 similar to GP|7211773|gb|AAF40430.1| protein kinase MK5 ... 108 5e-24
TC86213 homologue to PIR|S60121|S60121 mitogen-activated protein... 108 9e-24
TC77816 similar to GP|14532760|gb|AAK64081.1 putative kinase {Ar... 107 1e-23
TC86699 SP|P51137|MSK1_MEDSA Glycogen synthase kinase-3 homolog ... 106 3e-23
TC78167 homologue to GP|9294393|dbj|BAB02403.1 mitogen-activated... 103 2e-22
>TC90706 similar to PIR|E84825|E84825 probable protein kinase [imported] -
Arabidopsis thaliana, partial (38%)
Length = 756
Score = 417 bits (1073), Expect = e-117
Identities = 201/213 (94%), Positives = 208/213 (97%)
Frame = +3
Query: 391 ENKELPIVLNTVMAGRYYITEYLGSAAFSRVVQAHDLQTGTDVCLKIIKNDKDFFDQSLD 450
ENKE+PIVLN+VMAGRYY+TEYLGSAAFSRVVQAHDLQ G DVCLKIIKNDKDFFDQSLD
Sbjct: 3 ENKEVPIVLNSVMAGRYYVTEYLGSAAFSRVVQAHDLQMGIDVCLKIIKNDKDFFDQSLD 182
Query: 451 EIKLLKLVNKHDPADKHHILRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFT 510
EIKLLKLVNKHDP DKHHILRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFT
Sbjct: 183 EIKLLKLVNKHDPGDKHHILRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFT 362
Query: 511 LNRLQVITRQCLEALQYLHNLGIVHCDLKPENILIKSYKKCEIKVIDLGSSCFQTDNLCL 570
L RLQ+ITRQCLEALQYLHNLGIVHCDLKPEN+LIKSYKKCEIK+IDLGSSCF+TDNLCL
Sbjct: 363 LKRLQLITRQCLEALQYLHNLGIVHCDLKPENVLIKSYKKCEIKIIDLGSSCFKTDNLCL 542
Query: 571 YVQSRSYRAPEVMLGLEYDEKIDIWSLGCILAE 603
YVQSRSYRAPEVMLGL YDEKIDIWSLGCILAE
Sbjct: 543 YVQSRSYRAPEVMLGL*YDEKIDIWSLGCILAE 641
>TC80490 similar to PIR|E84825|E84825 probable protein kinase [imported] -
Arabidopsis thaliana, partial (7%)
Length = 798
Score = 233 bits (594), Expect = 2e-61
Identities = 123/171 (71%), Positives = 142/171 (82%), Gaps = 9/171 (5%)
Frame = +1
Query: 1 MAVPNGVEAVLQFLRKNGLSEAESALRQDIIEKNMYDDDLASFDYEKFFFPMVPPPPPVR 60
MAV N VEAV +FLRKNGLSEAESALRQDII+K +++DLASFDYEKFFFPMVPPPPPV+
Sbjct: 283 MAVSNSVEAVFEFLRKNGLSEAESALRQDIIDK--HNNDLASFDYEKFFFPMVPPPPPVK 456
Query: 61 LR----PSETPAAVVDCFESSSASSEDRFLSIGSATS----RVSSSEFINPYGIRSSSQT 112
LR PSE + +S+S SSE++F+SI S+TS RVSSSEFINPYGI SSSQT
Sbjct: 457 LRSFSRPSEFTSGDGQFSKSNSVSSEEQFVSIASSTSPSRSRVSSSEFINPYGINSSSQT 636
Query: 113 LNDSESSSDRLSQFGTARDYHDFEMQNEPYWYTEK-DEDFFMTPNFEGPDF 162
NDSESSSDRLSQFGTARDYH+F+MQ+EPYWY E D+D FMTP+F+GPDF
Sbjct: 637 QNDSESSSDRLSQFGTARDYHEFDMQHEPYWYNENDDDDDFMTPDFDGPDF 789
>BG646013 similar to GP|20260382|gb unknown protein {Arabidopsis thaliana},
partial (22%)
Length = 759
Score = 175 bits (444), Expect = 5e-44
Identities = 93/190 (48%), Positives = 123/190 (63%), Gaps = 3/190 (1%)
Frame = +2
Query: 436 KIIKNDKDFFDQSLDEIKLLKLVNK-HDPADKHHILRLYDYFYHQEHLFIVTELLRANLY 494
+IIKN ++ Q+L E+ +L +NK +DP DKHHI+R+YDYF +Q HL I ELL NLY
Sbjct: 86 RIIKNQPAYYQQALVEVTILTTLNKKYDPEDKHHIVRIYDYFVYQRHLCICFELLDTNLY 265
Query: 495 EFQKFNQESGGEAYFTLNRLQVITRQCLEALQYLHNLGIVHCDLKPENILI--KSYKKCE 552
E K N G +L +Q+ ++Q L L L + GI+HCDLKPENIL+ S K E
Sbjct: 266 ELIKMNHFRG----LSLGIVQLFSKQILCGLALLKDAGIIHCDLKPENILLCTSSVKSAE 433
Query: 553 IKVIDLGSSCFQTDNLCLYVQSRSYRAPEVMLGLEYDEKIDIWSLGCILAELCSGEVLFP 612
IK+ID GS+C + + Y+QSR YR+PEV+LG +Y ID+WS GCI+AEL G LFP
Sbjct: 434 IKIIDFGSACMENRTVYSYIQSRYYRSPEVLLGYQYTTAIDMWSFGCIVAELFLGLPLFP 613
Query: 613 NDSVALILAR 622
S +L R
Sbjct: 614 GASEFDLLKR 643
>TC80692 similar to GP|20260382|gb|AAM13089.1 unknown protein {Arabidopsis
thaliana}, partial (25%)
Length = 1388
Score = 155 bits (391), Expect = 7e-38
Identities = 87/207 (42%), Positives = 125/207 (60%), Gaps = 9/207 (4%)
Frame = +1
Query: 388 GFEE-NKELPIVLNTVM-----AGRYYITEYLGSAAFSRVVQAHDLQTGTDVCLKIIKND 441
GF+ N +L + +N V+ + RY + + LG F +V + + T + V +KIIKN
Sbjct: 739 GFDNANSDLILTVNLVLIHLEKSRRYIVKDLLGHGTFGQVAKCWNSDTNSFVAVKIIKNQ 918
Query: 442 KDFFDQSLDEIKLLKLVNK-HDPADKHHILRLYDYFYHQEHLFIVTELLRANLYEFQKFN 500
++ Q+L E+ +L +NK +DP DKHHI+R+YDYF +Q HL I ELL NLYE K N
Sbjct: 919 PAYYQQALVEVTILTTLNKKYDPEDKHHIVRIYDYFVYQRHLCICFELLDTNLYELIKMN 1098
Query: 501 QESGGEAYFTLNRLQVITRQCLEALQYLHNLGIVHCDLKPENILI--KSYKKCEIKVIDL 558
G +L +Q+ ++Q L L L + GI+HCDLKPENIL+ S K EIK+ID
Sbjct: 1099HFRG----LSLGIVQLFSKQILCGLALLKDAGIIHCDLKPENILLCTSSVKSAEIKIIDF 1266
Query: 559 GSSCFQTDNLCLYVQSRSYRAPEVMLG 585
GS+C + + Y+QSR Y+ V+LG
Sbjct: 1267GSACMENRTVYSYIQSRYYKIS*VLLG 1347
>TC91317 similar to PIR|C96761|C96761 hypothetical protein T9L24.35
[imported] - Arabidopsis thaliana, partial (10%)
Length = 765
Score = 154 bits (389), Expect = 1e-37
Identities = 69/120 (57%), Positives = 95/120 (78%)
Frame = +1
Query: 595 WSLGCILAELCSGEVLFPNDSVALILARMIGMLGPIDLEMLVKGKETHKYFTKEYDVYYA 654
WSLGCI AELC+G VLF NDS A +LAR+IG++GPI ML KG++T+KYFTK + +Y
Sbjct: 1 WSLGCIWAELCTGNVLFQNDSPATLLARVIGIIGPIGQSMLAKGRDTYKYFTKNHMLYER 180
Query: 655 NEETDQLEYIIPEESSLEQHLQVTDAMFIDFVKYLLNINPKRRPTTKQALKHPWLSHVYK 714
N+E+++LEY+IP+++SL L + D FIDFV +LL +NPK+RP+ +ALKHPWLS+ Y+
Sbjct: 181 NQESNRLEYLIPKKTSLRHRLPMGDQGFIDFVDHLLEVNPKKRPSASEALKHPWLSYPYE 360
>TC79563 similar to SP|P51567|AFC2_ARATH Protein kinase AFC2 (EC 2.7.1.-).
[Mouse-ear cress] {Arabidopsis thaliana}, partial (60%)
Length = 1307
Score = 131 bits (330), Expect = 8e-31
Identities = 86/258 (33%), Positives = 134/258 (51%), Gaps = 28/258 (10%)
Frame = +2
Query: 477 YHQEHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQCLEALQYLHNLGIVHC 536
+++ H+ IV E L LY+F + N F ++ ++ I RQ LE + ++H+L ++H
Sbjct: 20 HYRNHICIVFEKLGPILYDFLRKNNYRS----FPIDLVREIGRQLLECIAFMHDLRMIHT 187
Query: 537 DLKPENILI-----------------------KSYKKCEIKVIDLGSSCFQTDNLCLYVQ 573
DLKPENIL+ K K IKVID GS+ ++ + V
Sbjct: 188 DLKPENILLVSPDYVKVPDYKISSRSPNSYFKKVPKSSAIKVIDFGSTTYERVDQSYIVS 367
Query: 574 SRSYRAPEVMLGLEYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGMLGPIDLE 633
+R YRAPEV+LGL + DIWS+GCIL ELC+GE LF LA M +LGP+ +
Sbjct: 368 TRHYRAPEVILGLGWSHSCDIWSVGCILVELCTGEALFQTHENLEHLAMMERVLGPLPMH 547
Query: 634 MLVK-GKETHKYFTK---EYDVYYANEETDQLEYIIPE-ESSLEQHLQVTDAMFIDFVKY 688
ML + + KY + ++ V + E+ + +P ++ + QH+ + I ++
Sbjct: 548 MLKRVDRHAEKYVRRGKLDWPVGATSRESIKAVMKLPRLQNLIMQHVDHSAGDLIHLLQG 727
Query: 689 LLNINPKRRPTTKQALKH 706
LL +P R T K+AL+H
Sbjct: 728 LLRYDPSERITAKEALRH 781
>CA989663 similar to GP|14164406|db putative protein kinase AFC3 {Oryza
sativa (japonica cultivar-group)}, partial (53%)
Length = 749
Score = 127 bits (319), Expect = 1e-29
Identities = 82/253 (32%), Positives = 129/253 (50%), Gaps = 27/253 (10%)
Frame = +3
Query: 484 IVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQCLEALQYLHNLGIVHCDLKPENI 543
IV E L +L++F K N+ F ++ ++ RQ LE++ ++H L ++H DLKPENI
Sbjct: 3 IVFEKLGPSLFDFLKRNKYCP----FPVDLVREFGRQLLESVAFMHELRLIHTDLKPENI 170
Query: 544 LIKSY----------------------KKCEIKVIDLGSSCFQTDNLCLYVQSRSYRAPE 581
L+ S K IK+ID GS+ F + V +R YRAPE
Sbjct: 171 LLVSSDYVKLPSCKRVMSDETQFRCLPKSSAIKLIDFGSTAFANQSHSSIVSTRHYRAPE 350
Query: 582 VMLGLEYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGMLGPIDLEMLVKGKET 641
V+LGL + D+WS+GCIL ELC+G LF LA M +LGP+ M+ + K
Sbjct: 351 VILGLGWSSPCDLWSVGCILIELCTGGALFQTHENLEHLAMMERVLGPLPEHMVQRSKGY 530
Query: 642 HKYFTKEYDVYY-----ANEETDQLEYIIPEESSLEQHLQVTDAMFIDFVKYLLNINPKR 696
KYF + + + + E + ++ + + + +H++ + + D + LL P +
Sbjct: 531 GKYFKRGSRLRWPEGAVSRESINAVKKLGDLKDIISRHVESSRSSLTDLLYGLLTYEPSK 710
Query: 697 RPTTKQALKHPWL 709
R T +QAL HP+L
Sbjct: 711 RITARQALDHPFL 749
>TC78357 similar to GP|15289849|dbj|BAB63546. putative mitogen-activated
protein kinase {Oryza sativa (japonica cultivar-group)},
partial (69%)
Length = 1751
Score = 119 bits (299), Expect(2) = 8e-29
Identities = 87/260 (33%), Positives = 133/260 (50%), Gaps = 17/260 (6%)
Frame = +3
Query: 404 AGRYYITEYLGSAAFSRVVQAHDLQTGTDVCLKIIKNDKDFFDQS---LDEIKLLKLVNK 460
A RY I E +G ++ V A D TG V +K I + + + L EIKLL+L+
Sbjct: 522 ANRYKIQEVIGKGSYGVVCSAIDTHTGEKVAIKKIHDIFEHISDAARILREIKLLRLLRH 701
Query: 461 HDPADKHHILRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQ 520
D + HI+ L + +++V EL+ ++L++ K N + E Y Q Q
Sbjct: 702 PDIVEIKHIM-LPPSRKDFKDIYVVFELMESDLHQVIKANDDLTKEHY------QFFLYQ 860
Query: 521 CLEALQYLHNLGIVHCDLKPENILIKSYKKCEIKVIDLG----------SSCFQTDNLCL 570
L AL+Y+H + H DLKP+NIL + C++K+ D G ++ F TD
Sbjct: 861 LLRALKYIHTANVYHRDLKPKNILANA--NCKLKICDFGLARVAFSDTPTTVFWTD---- 1022
Query: 571 YVQSRSYRAPEVMLGL--EYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGMLG 628
YV +R YRAPE+ +Y IDIWS+GCI AE+ +G+ LFP +V L M +LG
Sbjct: 1023YVATRWYRAPELCGSFYSKYTPAIDIWSIGCIFAEVLTGKPLFPGKNVVHQLDLMTDLLG 1202
Query: 629 PIDLEML--VKGKETHKYFT 646
L+ + V+ ++ +Y T
Sbjct: 1203TPSLDTISRVRNEKARRYLT 1262
Score = 26.2 bits (56), Expect(2) = 8e-29
Identities = 12/31 (38%), Positives = 18/31 (57%)
Frame = +1
Query: 684 DFVKYLLNINPKRRPTTKQALKHPWLSHVYK 714
D+ K L +PK RPT ++AL P+ + K
Sbjct: 1330 DYWKDYLLFDPKDRPTAEEALADPYFKGLAK 1422
>BG587590 similar to PIR|T04125|T04 protein kinase PK12 (EC 2.7.1.-)
ethylene-induced - common tobacco, partial (57%)
Length = 780
Score = 124 bits (310), Expect = 2e-28
Identities = 82/263 (31%), Positives = 134/263 (50%), Gaps = 30/263 (11%)
Frame = +1
Query: 474 DYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQCLEALQYLHNLGI 533
++F ++ H+ IV E+L +LY+F + N F ++ ++ + RQ LE++ ++H++ +
Sbjct: 4 NWFDYRNHICIVFEMLGPSLYDFLRKNSYRP----FPVDLVRELGRQLLESVAFVHDMRL 171
Query: 534 VHCDLKPENILI---------------------KSYKKCE----IKVIDLGSSCFQTDNL 568
+H DLKPENIL SYK+ IKVID GS+ ++ +
Sbjct: 172 IHTDLKPENILFISPEYVKVPDYKVMFRSPKEGVSYKRLPKSSAIKVIDFGSTSYEHQDH 351
Query: 569 CLYVQSRSYRAPEVMLGLEYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGMLG 628
V +R YRAPEV+LGL ++ DIWS+GCIL ELCSGE LF LA M +LG
Sbjct: 352 NYIVSTRHYRAPEVILGLGWNFPCDIWSIGCILVELCSGEALFQTHENLEHLAMMERVLG 531
Query: 629 PIDLEMLVKGKETHKYFTKEYDVYY-----ANEETDQLEYIIPEESSLEQHLQVTDAMFI 683
PI ML + + + + + + + E + + + + QH+ + +
Sbjct: 532 PIPQHMLKRADHAAEKYVRRGRLNWPEGAVSRESIKAVLKLSRLPNLVMQHVDHSGGDLL 711
Query: 684 DFVKYLLNINPKRRPTTKQALKH 706
D ++ LL +P R +AL+H
Sbjct: 712 DLLQGLLRFDPMSRMKAHEALRH 780
>TC76803 PIR|T09622|T09622 protein kinase MMK4 (EC 2.7.1.-) cold- and
drought-induced - alfalfa, complete
Length = 1709
Score = 119 bits (297), Expect = 5e-27
Identities = 90/311 (28%), Positives = 147/311 (46%), Gaps = 10/311 (3%)
Frame = +2
Query: 413 LGSAAFSRVVQAHDLQTGTDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADKHHILRL 472
+G A+ V + +T V +K I N FD +D + L+ + D +++ L
Sbjct: 248 IGRGAYGIVCSLLNTETNELVAVKKIANA---FDNHMDAKRTLREIKLLRHLDHENVIGL 418
Query: 473 YDYF-----YHQEHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQCLEALQY 527
D ++I TEL+ +L++ + NQ E Q Q L L+Y
Sbjct: 419 RDVIPPPLRREFNDVYITTELMDTDLHQIIRSNQNLSDE------HCQYFLYQILRGLRY 580
Query: 528 LHNLGIVHCDLKPENILIKSYKKCEIKVIDLGSS--CFQTDNLCLYVQSRSYRAPEVML- 584
+H+ I+H DLKP N+L+ + C++K+ID G + ++D + YV +R YRAPE++L
Sbjct: 581 IHSANIIHRDLKPSNLLLNA--NCDLKIIDFGLARPTMESDFMTEYVVTRWYRAPELLLN 754
Query: 585 GLEYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGMLG-PIDLEM-LVKGKETH 642
+Y ID+WS+GCI EL + + LFP + + +LG P D ++ LVK +
Sbjct: 755 SSDYTSAIDVWSVGCIFMELMNKKPLFPGKDHVHQMRLLTELLGTPTDADVGLVKNDDAR 934
Query: 643 KYFTKEYDVYYANEETDQLEYIIPEESSLEQHLQVTDAMFIDFVKYLLNINPKRRPTTKQ 702
+Y + L + P L ID V +L I+P RR T ++
Sbjct: 935 RYIRQ-----LPQYPRQPLNRVFPHVHPLA----------IDLVDKMLTIDPTRRITVEE 1069
Query: 703 ALKHPWLSHVY 713
AL HP+L ++
Sbjct: 1070ALAHPYLEKLH 1102
>TC79500 similar to GP|9294059|dbj|BAB02016.1 MAP kinase {Arabidopsis
thaliana}, partial (55%)
Length = 1381
Score = 117 bits (294), Expect = 1e-26
Identities = 93/322 (28%), Positives = 152/322 (46%), Gaps = 17/322 (5%)
Frame = +2
Query: 404 AGRYYITEYLGSAAFSRVVQAHDLQTGTDVCLKIIKNDKDFFDQS---LDEIKLLKLVNK 460
A +Y + E +G ++ V A D TG V +K I + + + L EIKLL+L+
Sbjct: 422 ASQYQVQEIIGKGSYGVVCSAIDTHTGQKVAIKKINHVFEHVSDATRILREIKLLRLLKH 601
Query: 461 HDPADKHHILRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQ 520
D + HI+ L +++V EL+ ++L++ K N + T Q Q
Sbjct: 602 PDIVEIRHIM-LPPSRREFRDIYVVFELMESDLHQVIKANHD------LTPQHHQFFLYQ 760
Query: 521 CLEALQYLHNLGIVHCDLKPENILIKSYKKCEIKVIDLG----------SSCFQTDNLCL 570
L L+++H + H DLKP+NIL + C++K+ D G S+ F TD
Sbjct: 761 LLRGLKFIHTANVFHRDLKPKNILANA--DCKLKICDFGLARVSFNDAPSTIFWTD---- 922
Query: 571 YVQSRSYRAPEVMLGL--EYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGMLG 628
YV +R YRAPE+ +Y IDIWS+GCI AE+ +G LFP +V L M +LG
Sbjct: 923 YVATRWYRAPELCGSFFSKYTPGIDIWSIGCIFAEMLTGRPLFPGKNVVHQLDIMTDLLG 1102
Query: 629 --PIDLEMLVKGKETHKYFTKEYDVYYANEETDQLEYIIPEESSLEQHLQVTDAMFIDFV 686
P + ++ ++ +Y + + + +P Q D + + +
Sbjct: 1103TPPPESIARIRNEKARRYL-----------NSMRKKQPVP----FSQKFPNIDPLALRIL 1237
Query: 687 KYLLNINPKRRPTTKQALKHPW 708
+ LL +PK RP+ ++AL P+
Sbjct: 1238ERLLAFDPKNRPSAEEALSDPY 1303
>CA917722 similar to GP|14164406|db putative protein kinase AFC3 {Oryza
sativa (japonica cultivar-group)}, partial (52%)
Length = 858
Score = 117 bits (292), Expect = 2e-26
Identities = 71/200 (35%), Positives = 108/200 (53%), Gaps = 22/200 (11%)
Frame = +2
Query: 470 LRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQCLEALQYLH 529
+++ ++F ++ H+ IV E L +L++F K N+ F ++ ++ RQ LE++ ++H
Sbjct: 44 VQILNWFDYRNHICIVFEKLGPSLFDFLKRNKYCP----FPVDLVREFGRQLLESVAFMH 211
Query: 530 NLGIVHCDLKPENILIKSY----------------------KKCEIKVIDLGSSCFQTDN 567
L ++H DLKPENIL+ S K IK+ID GS+ F +
Sbjct: 212 ELRLIHTDLKPENILLVSSDYVKLPSCKRVMSDETQFRCLPKSSAIKLIDFGSTAFANQS 391
Query: 568 LCLYVQSRSYRAPEVMLGLEYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGML 627
V +R YRAPEV+LGL + D+WS+GCIL ELC+G LF LA M +L
Sbjct: 392 HSSIVSTRHYRAPEVILGLGWSSPCDLWSVGCILIELCTGGALFQTHENLEHLAMMERVL 571
Query: 628 GPIDLEMLVKGKETHKYFTK 647
GP+ M+ +G T KYF +
Sbjct: 572 GPLPEHMVQRG--TEKYFKR 625
>TC82909 homologue to GP|17064906|gb|AAL32607.1 Unknown protein {Arabidopsis
thaliana}, partial (67%)
Length = 1274
Score = 115 bits (288), Expect = 6e-26
Identities = 87/261 (33%), Positives = 130/261 (49%), Gaps = 20/261 (7%)
Frame = +2
Query: 406 RYYITEYLGSAAFSRVVQAHDLQTGTDVCLKIIKNDKDFFDQSLD------EIKLLKLVN 459
RY I E +G ++ V A+D TG V +K I D F+ D EIKLL+L+
Sbjct: 182 RYKIEEVIGKGSYGVVCSAYDTHTGEKVAIKKIN---DIFEHVSDATRILREIKLLRLLR 352
Query: 460 KHDPADKHHILRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITR 519
D + HIL L + +++V EL+ ++L++ K N + E Y Q
Sbjct: 353 HPDIVEIKHIL-LPPSRREFKDIYVVFELMESDLHQVIKANDDLTPEHY------QFFLY 511
Query: 520 QCLEALQYLHNLGIVHCDLKPENILIKSYKKCEIKVIDLG----------SSCFQTDNLC 569
Q L L+Y+H + H DLKP+NIL + C++K+ D G ++ F TD
Sbjct: 512 QLLRGLKYIHTANVFHRDLKPKNILANA--DCKLKICDFGLARVAFNDTPTAIFWTD--- 676
Query: 570 LYVQSRSYRAPEVMLGL--EYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGML 627
YV +R YRAPE+ +Y IDIWS+GCI AEL +G+ LFP +V L M L
Sbjct: 677 -YVATRWYRAPELCGSFFSKYTPAIDIWSIGCIFAELLTGKPLFPGKNVVHQLDIMTDFL 853
Query: 628 GPIDLEML--VKGKETHKYFT 646
G + + V+ ++ +Y +
Sbjct: 854 GTPSPDAIARVRNEKARRYLS 916
>TC87466 homologue to GP|10185114|emb|CAC08564. wound-induced GSK-3-like
protein {Medicago sativa}, complete
Length = 1934
Score = 109 bits (273), Expect = 3e-24
Identities = 116/478 (24%), Positives = 201/478 (41%), Gaps = 21/478 (4%)
Frame = +3
Query: 254 SFCKSRPGSGDSYGGDPTNFSYPDLKEIHLNDFHLKPAGDINSVNQSFDYYTKNVTGEGY 313
S R GGD + K + L+ K N+++++ + + E
Sbjct: 48 SIASGRTSISSDPGGDSNS------KRVKLDQETEKAYEGTNTLDRNDREQRVDSSNEAT 209
Query: 314 KGPYDLTIKVSETDLPNGLDPYETRDDLEFDEECHDPKVSSVGEDPNDDELLKYIQEDEY 373
G + +++T+ +G D E +E H+ K+ DE K E +
Sbjct: 210 VGTSNEVSAITKTEKKSGFD--------ELPKELHEMKIR--------DEKSKNNNEKDI 341
Query: 374 EVFDLRIIHRKNRTGFEENKELPIVLNTVMAGR---------YYITEYLGSAAFSRVVQA 424
E + + G E + ++ T +AGR Y +G+ +F V QA
Sbjct: 342 EATTV------SGNGTETGQ----IITTTIAGRDGQPKQTISYMAERVVGTGSFGVVFQA 491
Query: 425 HDLQTGTDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADKHHILRLYDYFYH-----Q 479
L+T V +K + DK + ++ L ++++ D +I++L FY +
Sbjct: 492 KCLETNEAVAIKKVLQDKRYKNRELQVMRMV---------DHPNIVKLKHCFYSTTEKDE 644
Query: 480 EHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQCLEALQYLHN-LGIVHCDL 538
+L +V E + +Y+ K + + +Q+ T Q L L YLH +G+ H D+
Sbjct: 645 LYLNLVLEYVPETVYKVSKNYIRM--HQHMPIIHVQLYTYQILRGLNYLHEVIGVCHRDI 818
Query: 539 KPENILIKSYKKCEIKVIDLGSSCFQT--DNLCLYVQSRSYRAPEVMLGL-EYDEKIDIW 595
KP+N+L+ + ++K+ D GS+ + Y+ SR YRAPE++ G EY ID+W
Sbjct: 819 KPQNLLVNAQTH-QLKICDFGSAKMLVPGEPNISYICSRYYRAPELIFGATEYTTAIDMW 995
Query: 596 SLGCILAELCSGEVLFPNDSVALILARMIGMLGPIDLEMLVKGKETHKYFTKEYDVYYAN 655
S+GC+LAEL G+ +FP +S L +I +LG +E + Y+
Sbjct: 996 SVGCVLAELLLGQAMFPGESGVDQLVEIIKVLG-------TPTREEIRCMNPNYN----- 1139
Query: 656 EETDQLEYIIPEESSLEQHLQVTDAM---FIDFVKYLLNINPKRRPTTKQALKHPWLS 710
E+ P+ + H M +D V LL +P R T A HP+ +
Sbjct: 1140------EFKFPQIKAHPWHKLFHKRMPSEAVDLVSRLLQYSPHLRCTALAACAHPFFN 1295
>TC77039 GP|313148|emb|CAA48472.1| protein kinase {Medicago sativa},
complete
Length = 1734
Score = 108 bits (271), Expect = 5e-24
Identities = 90/309 (29%), Positives = 138/309 (44%), Gaps = 7/309 (2%)
Frame = +2
Query: 407 YYITEYLGSAAFSRVVQAHDLQTGTDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADK 466
Y +G +F V QA L+TG V +K + DK + ++ L ++LL N
Sbjct: 452 YMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRLLDHPNVVSLKHC 631
Query: 467 HHILRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQCLEALQ 526
D Y L V E + + + K NQ + +++ + Q AL
Sbjct: 632 FFSTTEKDELYLNLVLEYVPETVSRVIRHYNKMNQR------MPMIYVKLYSYQICRALA 793
Query: 527 YLHN-LGIVHCDLKPENILIKSYKKCEIKVIDLGSS--CFQTDNLCLYVQSRSYRAPEVM 583
Y+HN +G+ H D+KP+N+L+ + ++K+ D GS+ + + Y+ SR YRAPE++
Sbjct: 794 YIHNSIGVCHRDIKPQNLLVNPHTH-QLKICDFGSAKVLVKGEPNISYICSRYYRAPELI 970
Query: 584 LGL-EYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGMLGPIDLEMLVKGKETH 642
G EY IDIWS GC+L EL G+ LFP +S L +I +LG +E
Sbjct: 971 FGATEYTTAIDIWSAGCVLGELLLGQPLFPGESGVDQLVEIIKVLG-------TPTREEI 1129
Query: 643 KYFTKEYDVYYANEETDQLEYIIPEESSLEQHLQVTDAM---FIDFVKYLLNINPKRRPT 699
K Y E+ P+ + H M +D V LL +P R T
Sbjct: 1130KCMNPNY-----------TEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRST 1276
Query: 700 TKQALKHPW 708
+AL HP+
Sbjct: 1277ALEALVHPF 1303
>TC83072 similar to GP|7211773|gb|AAF40430.1| protein kinase MK5
{Mesembryanthemum crystallinum}, partial (51%)
Length = 873
Score = 108 bits (271), Expect = 5e-24
Identities = 70/217 (32%), Positives = 112/217 (51%), Gaps = 28/217 (12%)
Frame = +2
Query: 520 QCLEALQYLHNLGIVHCDLKPENILIKSY-----------------------KKCEIKVI 556
+ LE + ++H+L ++H DLKPENIL+ S K IKVI
Sbjct: 2 ELLECVAFMHDLHMIHTDLKPENILLVSSEYLRIPDYKSSSRAPCSFYKRVPKSSAIKVI 181
Query: 557 DLGSSCFQTDNLCLYVQSRSYRAPEVMLGLEYDEKIDIWSLGCILAELCSGEVLFPNDSV 616
D GS+ ++ +N V +R YRAPEV+LGL + D+WS+GCIL ELC+GE LF
Sbjct: 182 DFGSTTYERENQNYIVSTRHYRAPEVILGLGWSYPCDVWSVGCILVELCTGEALFQTHEN 361
Query: 617 ALILARMIGMLGPIDLEMLVK-GKETHKYFTK---EYDVYYANEETDQLEYIIPE-ESSL 671
LA M +LG + ++L + + KY + ++ + E+ + +P ++ +
Sbjct: 362 LEHLAMMERVLGLLPQQLLKRVDRHAEKYVRRGRLDWPEGANSRESIKAVMKLPRLQNLI 541
Query: 672 EQHLQVTDAMFIDFVKYLLNINPKRRPTTKQALKHPW 708
QH+ + I ++ LL +P R T + AL+HP+
Sbjct: 542 MQHVDHSAGDLIHLLQGLLRYDPFERVTARDALRHPF 652
>TC86213 homologue to PIR|S60121|S60121 mitogen-activated protein kinase
MMK2 (EC 2.7.1.-) - alfalfa, complete
Length = 1596
Score = 108 bits (269), Expect = 9e-24
Identities = 80/310 (25%), Positives = 149/310 (47%), Gaps = 9/310 (2%)
Frame = +2
Query: 413 LGSAAFSRVVQAHDLQTGTDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADKHHILRL 472
+G A+ V A + +T +V +K I N FD +D + L+ + D +++ +
Sbjct: 368 VGRGAYGIVCAAVNAETREEVAIKKIGNA---FDNRIDAKRTLREIKLLRHMDHENVMSI 538
Query: 473 YDYFYHQEH-----LFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQCLEALQY 527
D + ++IV+EL+ +L++ + NQ T + + Q L L+Y
Sbjct: 539 KDIIRPPQKENFNDVYIVSELMDTDLHQIIRSNQP------MTDDHCRYFVYQLLRGLKY 700
Query: 528 LHNLGIVHCDLKPENILIKSYKKCEIKVIDLG--SSCFQTDNLCLYVQSRSYRAPEVMLG 585
+H+ ++H DLKP N+L+ + C++K+ D G + +TD + YV +R YRAPE++L
Sbjct: 701 VHSANVLHRDLKPSNLLLNA--NCDLKIGDFGLARTTSETDFMTEYVVTRWYRAPELLLN 874
Query: 586 L-EYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGMLG-PIDLEMLVKGKETHK 643
EY IDIWS+GCIL E+ + + LFP L + ++G P D + E +
Sbjct: 875 CSEYTAAIDIWSVGCILGEIVTRQPLFPGRDYVHQLRLVTELIGSPDDASLGFLRSENAR 1054
Query: 644 YFTKEYDVYYANEETDQLEYIIPEESSLEQHLQVTDAMFIDFVKYLLNINPKRRPTTKQA 703
+ ++ Y + + + P +D ++ +L +P +R +A
Sbjct: 1055RYVRQLPQYPQQNFSTRFPSMSPGA--------------VDLLEKMLIFDPSKRIRVDEA 1192
Query: 704 LKHPWLSHVY 713
L HP+++ ++
Sbjct: 1193LCHPYMAPLH 1222
>TC77816 similar to GP|14532760|gb|AAK64081.1 putative kinase {Arabidopsis
thaliana}, partial (75%)
Length = 2695
Score = 107 bits (268), Expect = 1e-23
Identities = 88/313 (28%), Positives = 151/313 (48%), Gaps = 5/313 (1%)
Frame = +3
Query: 406 RYYITEYLGSAAFSRVVQAHDLQTGTDVCLKIIKNDKDFFDQ--SLDEIKLLKLVNKHDP 463
R + E+LG + + +A G V +K +K +++ +L E+K L+ +N +
Sbjct: 1026 RKLVMEHLGLSGEPLISKA-----GEVVAIKKMKKKYYSWEECVNLREVKSLRKMNHSN- 1187
Query: 464 ADKHHILRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQCLE 523
+++L + + L++V E + NLY+ K E F+ + ++ + Q +
Sbjct: 1188 -----VVKLKEVIRESDILYLVFEYMECNLYQLMKKR-----EKLFSDDEVRNLCFQVFQ 1337
Query: 524 ALQYLHNLGIVHCDLKPENILIKSYKKCEIKVIDLG--SSCFQTDNLCLYVQSRSYRAPE 581
L Y+H G H DLKPEN+L+ K IKV D G YV +R YRAPE
Sbjct: 1338 GLAYMHQRGYFHRDLKPENLLVT---KDIIKVSDFGLVREISSQPPYTEYVSTRWYRAPE 1508
Query: 582 VMLGLE-YDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGMLGPIDLEMLVKGKE 640
V+L Y K+D+W++G I+AEL + LFP S A + ++ G++G E G +
Sbjct: 1509 VLLQSSLYSSKVDMWAMGAIMAELFTLRPLFPGTSEADEIYKICGVIGSPTAESWADGLK 1688
Query: 641 THKYFTKEYDVYYANEETDQLEYIIPEESSLEQHLQVTDAMFIDFVKYLLNINPKRRPTT 700
+++ + + T L +IP S DA I+ +K L + +P +RP+
Sbjct: 1689 ----LSRDINYQFPQVATADLSVLIPSRSD--------DA--INLIKSLCSWDPCKRPSA 1826
Query: 701 KQALKHPWLSHVY 713
+AL+HP+ +
Sbjct: 1827 AEALQHPFFKSCF 1865
>TC86699 SP|P51137|MSK1_MEDSA Glycogen synthase kinase-3 homolog MsK-1 (EC
2.7.1.-). [Alfalfa] {Medicago sativa}, complete
Length = 1616
Score = 106 bits (265), Expect = 3e-23
Identities = 88/313 (28%), Positives = 138/313 (43%), Gaps = 7/313 (2%)
Frame = +1
Query: 407 YYITEYLGSAAFSRVVQAHDLQTGTDVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPADK 466
Y +G +F V QA L+TG V +K + DK + ++ L ++LL N
Sbjct: 358 YMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRLLDHPNVVSLKHC 537
Query: 467 HHILRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGEAYFTLNRLQVITRQCLEALQ 526
D Y L V E + + + K NQ + +++ T Q AL
Sbjct: 538 FFSTTEKDELYLNLVLEYVPETVHRVIKHYSKLNQR------MPMIYVKLYTYQIFRALS 699
Query: 527 YLHN-LGIVHCDLKPENILIKSYKKCEIKVIDLGSS--CFQTDNLCLYVQSRSYRAPEVM 583
Y+H +G+ H D+KP+N+L+ + ++K+ D GS+ + + Y+ SR YRAPE++
Sbjct: 700 YIHRCIGVCHRDIKPQNLLVNPHTH-QVKLCDFGSAKVLVKGEPNISYICSRYYRAPELI 876
Query: 584 LGL-EYDEKIDIWSLGCILAELCSGEVLFPNDSVALILARMIGMLGPIDLEMLVKGKETH 642
G EY ID+WS+GC+LAEL G+ LFP +S L +I +LG +E
Sbjct: 877 FGATEYTTAIDVWSVGCVLAELLLGQPLFPGESGVDQLVEIIKVLG-------TPTREEI 1035
Query: 643 KYFTKEYDVYYANEETDQLEYIIPEESSLEQHL---QVTDAMFIDFVKYLLNINPKRRPT 699
K Y E+ P+ + H + A +D V LL +P R
Sbjct: 1036KCMNPNY-----------TEFKFPQIKAHPWHKIFHKRMPAEAVDLVSRLLQYSPNLRCQ 1182
Query: 700 TKQALKHPWLSHV 712
L HP+ +
Sbjct: 1183ALDCLTHPFFDEL 1221
>TC78167 homologue to GP|9294393|dbj|BAB02403.1 mitogen-activated protein
kinase {Arabidopsis thaliana}, partial (36%)
Length = 927
Score = 103 bits (257), Expect = 2e-22
Identities = 76/230 (33%), Positives = 115/230 (49%), Gaps = 15/230 (6%)
Frame = +1
Query: 390 EENKELPIVLNTVMAGRYYITEYLGSAAFSRVVQAHDLQTGTDVCLKIIKNDKDFFDQS- 448
+ +KEL A RY I E +G ++ V A D TG V +K I++ + +
Sbjct: 271 KNSKELEFFTEYGEANRYKIIEIIGKGSYGVVCAAIDTHTGEKVAIKKIQDAFEHISDAV 450
Query: 449 --LDEIKLLKLVNKHDPADKHHILRLYDYFYHQEHLFIVTELLRANLYEFQKFNQESGGE 506
L E+KLL+L+ D D I+ L + +++V EL+ ++L++ K N +
Sbjct: 451 RILREVKLLRLLRHPDIVDIKRIM-LPPSKREFKDIYVVFELMESDLHQVIKANDD---- 615
Query: 507 AYFTLNRLQVITRQCLEALQYLHNLGIVHCDLKPENILIKSYKKCEIKVIDLG------- 559
T Q Q L AL+Y+H+ + H DLKP+NIL + C++KV D G
Sbjct: 616 --LTREHHQFFLYQMLRALKYMHSANVYHRDLKPKNILANA--NCKLKVCDFGLARVAFN 783
Query: 560 ---SSCFQTDNLCLYVQSRSYRAPEV--MLGLEYDEKIDIWSLGCILAEL 604
+S F TD YV +R YRAPE+ +Y ID+WS+GCI AE+
Sbjct: 784 DAPTSIFWTD----YVATRWYRAPELCGSFASKYTPAIDLWSIGCIFAEV 921
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.137 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,426,463
Number of Sequences: 36976
Number of extensions: 366903
Number of successful extensions: 3096
Number of sequences better than 10.0: 384
Number of HSP's better than 10.0 without gapping: 2697
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2900
length of query: 716
length of database: 9,014,727
effective HSP length: 103
effective length of query: 613
effective length of database: 5,206,199
effective search space: 3191399987
effective search space used: 3191399987
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0204.9