BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0201.11
(131 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG455075 similar to GP|5139541|emb nodulin26-like major intrinsi... 171 6e-44
TC77955 similar to GP|5139541|emb|CAB45652.1 nodulin26-like majo... 156 3e-39
TC86110 multifunctional aquaporin [Medicago truncatula] 144 1e-35
TC79264 similar to PIR|B96840|B96840 hypothetical protein F23A5.... 87 1e-18
TC93480 similar to PIR|B96840|B96840 hypothetical protein F23A5.... 85 9e-18
BG644707 homologue to PIR|T04053|T04 nodulin-26 homolog F24G24.1... 69 5e-13
TC77008 similar to PIR|A84653|A84653 hypothetical protein At2g25... 53 4e-08
TC76601 aquaporin-like protein PIP2 [Medicago truncatula] 41 2e-04
TC77007 similar to PIR|T10804|T10804 tonoplast intrinsic protein... 40 2e-04
TC88670 similar to GP|535780|dbj|BAA05654.1| transmembrane prote... 40 2e-04
TC85305 aquaporin 40 3e-04
TC85259 homologue to PIR|T09260|T09260 aquaporin-like transmembr... 40 3e-04
TC89494 homologue to GP|13447793|gb|AAK26754.1 plasma membrane i... 39 5e-04
TC78780 similar to PIR|JQ1106|JQ1106 tonoplast intrinsic protein... 39 6e-04
TC77711 similar to PIR|T46160|T46160 caffeic acid O-methyltransf... 39 8e-04
TC78388 homologue to GP|8071620|gb|AAF71816.1| putative aquapori... 38 0.001
TC77005 similar to SP|P21653|TIP1_TOBAC Tonoplast intrinsic prot... 38 0.001
TC85260 homologue to GP|3158476|gb|AAC17529.1| aquaporin 2 {Sama... 38 0.001
TC76539 aquaporin [Medicago truncatula] 36 0.004
AW736472 homologue to SP|P21653|TIP1 Tonoplast intrinsic protein... 35 0.007
>BG455075 similar to GP|5139541|emb nodulin26-like major intrinsic protein
{Pisum sativum}, partial (59%)
Length = 652
Score = 171 bits (434), Expect = 6e-44
Identities = 88/125 (70%), Positives = 104/125 (82%), Gaps = 2/125 (1%)
Frame = +3
Query: 1 MADYSGSN-EVILNVNNETSKKCDSIE-EDCVPLLQKLVAEVLGTYFLIFAGCASVVVNL 58
M D S N +V++N+N++ +KKCD +D VPLLQKLVAEV+GT+FLIFAGCA+VVVNL
Sbjct: 69 MGDISNGNLDVVMNINDDATKKCDDTTIDDHVPLLQKLVAEVVGTFFLIFAGCAAVVVNL 248
Query: 59 NNDKVVTLPGIAIVWGLAVMVLVYSIGHISGAHFNPAVTVAHATTKSFPVKQVFPLPLFK 118
NNDKVVTLPGI+IVWGLAVMVLVYSIGHISGAHFNPAVT+AH TT FP+KQ+ P +
Sbjct: 249 NNDKVVTLPGISIVWGLAVMVLVYSIGHISGAHFNPAVTIAHTTTGRFPLKQL-PAYIIA 425
Query: 119 HVGGS 123
V GS
Sbjct: 426 QVVGS 440
>TC77955 similar to GP|5139541|emb|CAB45652.1 nodulin26-like major intrinsic
protein {Pisum sativum}, partial (97%)
Length = 1272
Score = 156 bits (394), Expect = 3e-39
Identities = 81/115 (70%), Positives = 93/115 (80%), Gaps = 4/115 (3%)
Frame = +1
Query: 1 MADYSGSN---EVILNVNNETSKKC-DSIEEDCVPLLQKLVAEVLGTYFLIFAGCASVVV 56
MA+ S SN E++LNVN + S K DS LQKLVAEV+GTYFLIFAGCASV+V
Sbjct: 145 MAENSASNATNEIVLNVNKDVSNKSEDSTSHATASFLQKLVAEVIGTYFLIFAGCASVLV 324
Query: 57 NLNNDKVVTLPGIAIVWGLAVMVLVYSIGHISGAHFNPAVTVAHATTKSFPVKQV 111
N NN+ VVTLPGI+IVWGLAVMVLVYS+GHISGAHFNPAVT+A A+TK FP+KQV
Sbjct: 325 NKNNENVVTLPGISIVWGLAVMVLVYSLGHISGAHFNPAVTIAFASTKRFPLKQV 489
>TC86110 multifunctional aquaporin [Medicago truncatula]
Length = 1411
Score = 144 bits (363), Expect = 1e-35
Identities = 70/106 (66%), Positives = 88/106 (82%), Gaps = 1/106 (0%)
Frame = +3
Query: 7 SNEVILNVNNETSKKCD-SIEEDCVPLLQKLVAEVLGTYFLIFAGCASVVVNLNNDKVVT 65
++EV+L+ N ++S C S VP LQKL+AE++GTYFLIFAGCAS+VVN +ND VVT
Sbjct: 99 THEVVLDTNKDSSDTCKGSGSFVSVPFLQKLIAEMVGTYFLIFAGCASIVVNKDNDNVVT 278
Query: 66 LPGIAIVWGLAVMVLVYSIGHISGAHFNPAVTVAHATTKSFPVKQV 111
LPGIAIVWGL ++VL+YS+GHISGAHFNPAVT+A ATT+ FP+ QV
Sbjct: 279 LPGIAIVWGLTLLVLIYSLGHISGAHFNPAVTIAFATTRRFPLLQV 416
>TC79264 similar to PIR|B96840|B96840 hypothetical protein F23A5.11
[imported] - Arabidopsis thaliana, partial (82%)
Length = 861
Score = 87.4 bits (215), Expect = 1e-18
Identities = 48/93 (51%), Positives = 60/93 (63%)
Frame = +2
Query: 30 VPLLQKLVAEVLGTYFLIFAGCASVVVNLNNDKVVTLPGIAIVWGLAVMVLVYSIGHISG 89
VPL +K+ AE +GTY L+FAG A+ +VN TL G A GLAVM+++ S GHISG
Sbjct: 242 VPLAKKVGAEFIGTYILMFAGIATAIVNQKIHNSETLIGCAGATGLAVMIIILSTGHISG 421
Query: 90 AHFNPAVTVAHATTKSFPVKQVFPLPLFKHVGG 122
AH NPAVT++ A K FP K V PL + V G
Sbjct: 422 AHLNPAVTISFAALKHFPWKNV-PLDIAAQVFG 517
>TC93480 similar to PIR|B96840|B96840 hypothetical protein F23A5.11
[imported] - Arabidopsis thaliana, partial (78%)
Length = 902
Score = 84.7 bits (208), Expect = 9e-18
Identities = 43/82 (52%), Positives = 54/82 (65%)
Frame = +3
Query: 30 VPLLQKLVAEVLGTYFLIFAGCASVVVNLNNDKVVTLPGIAIVWGLAVMVLVYSIGHISG 89
VPL +K+ AE +GT L+FAG A+ +VN TL G A GLAVM+++ S GHISG
Sbjct: 288 VPLAKKIGAEFIGTLILMFAGAATAIVNQKTQGSETLIGCATSTGLAVMIIILSTGHISG 467
Query: 90 AHFNPAVTVAHATTKSFPVKQV 111
AH NPAVT++ A K FP K V
Sbjct: 468 AHLNPAVTISFAALKHFPWKHV 533
>BG644707 homologue to PIR|T04053|T04 nodulin-26 homolog F24G24.180 -
Arabidopsis thaliana, partial (39%)
Length = 731
Score = 68.9 bits (167), Expect = 5e-13
Identities = 35/73 (47%), Positives = 47/73 (63%)
Frame = +2
Query: 39 EVLGTYFLIFAGCASVVVNLNNDKVVTLPGIAIVWGLAVMVLVYSIGHISGAHFNPAVTV 98
E +GT+ LIFA A +VN + +L G A GLAVM+++ S GHISGAH NP++T+
Sbjct: 371 EFVGTFILIFAATAGPIVNQKYNGAESLIGNAACSGLAVMIVILSTGHISGAHLNPSLTI 550
Query: 99 AHATTKSFPVKQV 111
A A + FP QV
Sbjct: 551 AFAALRHFPWVQV 589
>TC77008 similar to PIR|A84653|A84653 hypothetical protein At2g25810
[imported] - Arabidopsis thaliana, partial (97%)
Length = 967
Score = 52.8 bits (125), Expect = 4e-08
Identities = 30/78 (38%), Positives = 45/78 (57%), Gaps = 2/78 (2%)
Frame = +2
Query: 23 DSIEEDCVPLLQKLVAEVLGTYFLIFAGCASVVV--NLNNDKVVTLPGIAIVWGLAVMVL 80
++ + DC+ Q L+ E + T+ +FAG S + L+ D +V L +AI L V V+
Sbjct: 53 EATQPDCI---QALIVEFIATFLFVFAGVGSAMTADKLSGDALVGLFFVAIAHALVVAVM 223
Query: 81 VYSIGHISGAHFNPAVTV 98
+ S HISG H NPAVT+
Sbjct: 224 I-SAAHISGGHLNPAVTL 274
>TC76601 aquaporin-like protein PIP2 [Medicago truncatula]
Length = 1497
Score = 40.8 bits (94), Expect = 2e-04
Identities = 23/64 (35%), Positives = 34/64 (52%), Gaps = 3/64 (4%)
Frame = +2
Query: 37 VAEVLGTYFLIFAGCASVVVNLNND---KVVTLPGIAIVWGLAVMVLVYSIGHISGAHFN 93
+AE + T+ ++ +V+ + D K V + GIA +G + LVY ISG H N
Sbjct: 479 IAEFVATFLFLYITVLTVMGVVRADSKCKTVGIQGIAWAFGGMIFALVYCTAGISGGHIN 658
Query: 94 PAVT 97
PAVT
Sbjct: 659 PAVT 670
>TC77007 similar to PIR|T10804|T10804 tonoplast intrinsic protein delta
type - upland cotton, complete
Length = 1041
Score = 40.4 bits (93), Expect = 2e-04
Identities = 24/70 (34%), Positives = 36/70 (51%), Gaps = 5/70 (7%)
Frame = +2
Query: 33 LQKLVAEVLGTYFLIFAGCASVVV--NLNNDKVVTLPG---IAIVWGLAVMVLVYSIGHI 87
++ +AE + T +FAG S + L +D + G +A+ G A+ V V +I
Sbjct: 128 IKAYIAEFISTLLFVFAGVGSAIAYGKLTSDAALDPAGLLAVAVCHGFALFVAVAVGANI 307
Query: 88 SGAHFNPAVT 97
SG H NPAVT
Sbjct: 308 SGGHVNPAVT 337
>TC88670 similar to GP|535780|dbj|BAA05654.1| transmembrane protein
{Arabidopsis thaliana}, complete
Length = 1106
Score = 40.4 bits (93), Expect = 2e-04
Identities = 24/64 (37%), Positives = 37/64 (57%), Gaps = 3/64 (4%)
Frame = +1
Query: 37 VAEVLGTYFLIFAGCASVV-VNLNNDKV--VTLPGIAIVWGLAVMVLVYSIGHISGAHFN 93
+AE + T+ ++ +V+ VN +++K V + GIA +G + LVY ISG H N
Sbjct: 217 IAEFVATFLFLYITVLTVMGVNKSSNKCSSVGVQGIAWAFGGMIFALVYCTAGISGGHIN 396
Query: 94 PAVT 97
PAVT
Sbjct: 397 PAVT 408
>TC85305 aquaporin
Length = 1221
Score = 40.0 bits (92), Expect = 3e-04
Identities = 25/66 (37%), Positives = 34/66 (50%), Gaps = 5/66 (7%)
Frame = +2
Query: 37 VAEVLGTYFLIFAGCASVVV--NLNNDKVVTLPGI---AIVWGLAVMVLVYSIGHISGAH 91
+AE + T+ +FAG S + L ND T G+ +I A+ V V +ISG H
Sbjct: 167 LAEFISTFIFVFAGSGSGIAYNKLTNDGAATPAGLISASIAHAFALFVAVSVGANISGGH 346
Query: 92 FNPAVT 97
NPAVT
Sbjct: 347 VNPAVT 364
>TC85259 homologue to PIR|T09260|T09260 aquaporin-like transmembrane channel
protein - alfalfa, complete
Length = 1502
Score = 39.7 bits (91), Expect = 3e-04
Identities = 24/64 (37%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
Frame = +3
Query: 37 VAEVLGTYFLIFAGCASVV-VNLNNDKVVT--LPGIAIVWGLAVMVLVYSIGHISGAHFN 93
+AE + T+ ++ +V+ VN + K T + GIA +G + LVY ISG H N
Sbjct: 252 IAEFVATFLFLYITILTVMGVNRSESKCKTVGIQGIAWSFGGMIFALVYCTAGISGGHIN 431
Query: 94 PAVT 97
PAVT
Sbjct: 432 PAVT 443
>TC89494 homologue to GP|13447793|gb|AAK26754.1 plasma membrane integral
protein ZmPIP1-3 {Zea mays}, partial (93%)
Length = 1132
Score = 39.3 bits (90), Expect = 5e-04
Identities = 24/64 (37%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
Frame = +1
Query: 37 VAEVLGTYFLIFAGCASVV-VNLNNDKV--VTLPGIAIVWGLAVMVLVYSIGHISGAHFN 93
+AE + T+ ++ +V+ VN + K V + GIA +G + LVY ISG H N
Sbjct: 205 IAEFVATFLFLYITILTVMGVNRSTSKCSSVGIQGIAWSFGGMIFALVYCTAGISGGHIN 384
Query: 94 PAVT 97
PAVT
Sbjct: 385 PAVT 396
>TC78780 similar to PIR|JQ1106|JQ1106 tonoplast intrinsic protein alpha -
kidney bean, partial (95%)
Length = 999
Score = 38.9 bits (89), Expect = 6e-04
Identities = 23/70 (32%), Positives = 35/70 (49%), Gaps = 5/70 (7%)
Frame = +1
Query: 33 LQKLVAEVLGTYFLIFAGCAS--VVVNLNNDKVVT---LPGIAIVWGLAVMVLVYSIGHI 87
++ +AE T+ +FAG S +V + D + L +A+ A+ V S H+
Sbjct: 76 IRATIAEFASTFIFVFAGEGSGLALVKIYQDSAFSAGELLAVALAHAFALFAAVSSSMHV 255
Query: 88 SGAHFNPAVT 97
SG H NPAVT
Sbjct: 256 SGGHVNPAVT 285
>TC77711 similar to PIR|T46160|T46160 caffeic acid O-methyltransferase-like
protein - Arabidopsis thaliana, partial (86%)
Length = 2361
Score = 38.5 bits (88), Expect = 8e-04
Identities = 25/73 (34%), Positives = 36/73 (49%), Gaps = 5/73 (6%)
Frame = +2
Query: 30 VPLLQKLVAEVLGTYFLIFAGCASVVV--NLNNDKVVTLPG---IAIVWGLAVMVLVYSI 84
V ++ VAE + T +FAG S + L + + G +A+ G A+ V V
Sbjct: 113 VSSIRAYVAEFISTLIFVFAGVGSAIAYAKLTSGAALDPAGLVAVAVCHGFALFVAVSVG 292
Query: 85 GHISGAHFNPAVT 97
+ISG H NPAVT
Sbjct: 293 ANISGGHVNPAVT 331
>TC78388 homologue to GP|8071620|gb|AAF71816.1| putative aquaporin PIP2-1
{Vitis berlandieri x Vitis rupestris}, partial (91%)
Length = 1120
Score = 38.1 bits (87), Expect = 0.001
Identities = 25/71 (35%), Positives = 34/71 (47%), Gaps = 9/71 (12%)
Frame = +2
Query: 36 LVAEVLGTYFLIFAGCASVVV---------NLNNDKVVTLPGIAIVWGLAVMVLVYSIGH 86
L+AE + T ++ +V+ N N V + GIA +G + VLVY
Sbjct: 152 LIAEFVATLLFLYVTVLTVIGYNAQTDPAHNGTNCDGVGILGIAWAFGGMIFVLVYCTAG 331
Query: 87 ISGAHFNPAVT 97
ISG H NPAVT
Sbjct: 332 ISGGHINPAVT 364
>TC77005 similar to SP|P21653|TIP1_TOBAC Tonoplast intrinsic protein
root-specific RB7-5A (RT-TIP). [Common tobacco]
{Nicotiana tabacum}, partial (97%)
Length = 1055
Score = 38.1 bits (87), Expect = 0.001
Identities = 23/70 (32%), Positives = 36/70 (50%), Gaps = 5/70 (7%)
Frame = +1
Query: 33 LQKLVAEVLGTYFLIFAGCASVVV--NLNNDKVVTLPG---IAIVWGLAVMVLVYSIGHI 87
L+ ++E + T +FAG S + +L +D + G +A+ A+ V V +I
Sbjct: 163 LKAYLSEFIATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANI 342
Query: 88 SGAHFNPAVT 97
SG H NPAVT
Sbjct: 343 SGGHLNPAVT 372
>TC85260 homologue to GP|3158476|gb|AAC17529.1| aquaporin 2 {Samanea saman},
complete
Length = 1282
Score = 37.7 bits (86), Expect = 0.001
Identities = 25/71 (35%), Positives = 34/71 (47%), Gaps = 9/71 (12%)
Frame = +3
Query: 36 LVAEVLGTYFLIFAGCASVV-------VNLNNDKV--VTLPGIAIVWGLAVMVLVYSIGH 86
L+AE + T ++ +V+ V D V + GIA +G + VLVY
Sbjct: 207 LIAEFIATLLFLYVTVLTVIGYSIQTDVKAGGDACGGVGILGIAWAFGGMIFVLVYCTAG 386
Query: 87 ISGAHFNPAVT 97
ISG H NPAVT
Sbjct: 387 ISGGHINPAVT 419
>TC76539 aquaporin [Medicago truncatula]
Length = 1423
Score = 36.2 bits (82), Expect = 0.004
Identities = 31/112 (27%), Positives = 49/112 (43%), Gaps = 16/112 (14%)
Frame = +1
Query: 32 LLQKLVAEVLGTYFLIFAGCASVVV-----------NLNNDKVVTLPGIAIVWGLAVMVL 80
L + ++AE + T ++ +++ N D V L GIA +G + +L
Sbjct: 265 LYRAVIAEFVATLLFLYITVLTIIGYSRQSDTTIKGNTECDGVGVL-GIAWAFGGMIFIL 441
Query: 81 VYSIGHISGAHFNPAVTVAHATTKSFPVKQVFPLPLFKHVG-----GSAKGF 127
VY ISG H NPAVT + + + F + + +G G AKGF
Sbjct: 442 VYCTAGISGGHINPAVTFGLFVGRKVSLIRAFLYIVAQCLGAICGAGLAKGF 597
>AW736472 homologue to SP|P21653|TIP1 Tonoplast intrinsic protein
root-specific RB7-5A (RT-TIP). [Common tobacco]
{Nicotiana tabacum}, partial (43%)
Length = 691
Score = 35.4 bits (80), Expect = 0.007
Identities = 22/65 (33%), Positives = 32/65 (48%), Gaps = 5/65 (7%)
Frame = -2
Query: 38 AEVLGTYFLIFAGCASVVV--NLNNDKVVTLPG---IAIVWGLAVMVLVYSIGHISGAHF 92
A + T +FAG S + +L +D + G +A+ A+ V V +ISG H
Sbjct: 606 ARCVATLIFVFAGVGSAIAYNDLTSDAALDPAGLVAVAVAHAFALFVGVAIAANISGGHL 427
Query: 93 NPAVT 97
NPAVT
Sbjct: 426 NPAVT 412
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.138 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,203,722
Number of Sequences: 36976
Number of extensions: 50993
Number of successful extensions: 281
Number of sequences better than 10.0: 55
Number of HSP's better than 10.0 without gapping: 278
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 278
length of query: 131
length of database: 9,014,727
effective HSP length: 85
effective length of query: 46
effective length of database: 5,871,767
effective search space: 270101282
effective search space used: 270101282
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0201.11