BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0197.6
(158 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77214 similar to PIR|T11855|T11855 DnaJ protein homolog - kidn... 159 3e-40
TC77215 similar to PIR|T11855|T11855 DnaJ protein homolog - kidn... 158 1e-39
TC83173 similar to PIR|T11855|T11855 DnaJ protein homolog - kidn... 156 4e-39
BQ140212 similar to PIR|T11855|T118 DnaJ protein homolog - kidne... 100 2e-22
TC79039 similar to GP|9294537|dbj|BAB02800.1 DnaJ-like protein {... 87 3e-18
TC80547 similar to GP|7268087|emb|CAB78425.1 DnaJ-like protein {... 70 3e-13
TC89246 homologue to PIR|T06594|T06594 heat shock protein dnaJ -... 64 3e-11
TC86700 similar to GP|18377835|gb|AAL67104.1 At1g24120/F3I6_4 {A... 61 2e-10
TC77797 similar to GP|6714354|gb|AAF26045.1| ARG1 protein (Alter... 59 8e-10
TC77858 similar to GP|20197886|gb|AAD22362.2 putative DnaJ prote... 59 1e-09
AJ503880 similar to GP|12838381|dbj DnaJ (Hsp40) homolog subfam... 58 1e-09
BG452391 weakly similar to GP|22135874|gb| DnaJ protein putativ... 57 3e-09
TC85313 PIR|T09338|T09338 DnaJ-like protein MsJ1 - alfalfa, comp... 57 4e-09
TC85315 similar to GP|9758827|dbj|BAB09499.1 gene_id:MTH12.18~un... 57 4e-09
TC90060 similar to GP|23428796|gb|AAM23263.1 DnaJ-like protein {... 56 5e-09
TC87379 similar to PIR|T04618|T04618 heat shock protein homolog ... 56 5e-09
TC90074 similar to PIR|G84590|G84590 probable heat shock protein... 56 5e-09
TC91543 similar to PIR|H96808|H96808 protein F28K19.14 [imported... 56 7e-09
TC78760 similar to PIR|T48161|T48161 heat shock protein 40-like ... 55 9e-09
TC78759 similar to GP|21618097|gb|AAM67147.1 putative heat shock... 55 9e-09
>TC77214 similar to PIR|T11855|T11855 DnaJ protein homolog - kidney bean
(fragment), partial (61%)
Length = 750
Score = 159 bits (403), Expect = 3e-40
Identities = 95/165 (57%), Positives = 114/165 (68%), Gaps = 8/165 (4%)
Frame = +1
Query: 2 LSPSFTTATTFSGQIATSPTGHVRSRPVLAFATATE-ADYRPT------PSLHLSTHGDS 54
L S A FSG T P VRS+P+L A+AT A+ R T PS + +S
Sbjct: 58 LPSSLYAAGNFSGATVTPPPCRVRSKPILITASATATAEARSTWTEQQRPSY---LNMNS 228
Query: 55 GMASCSSSLYEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYS 114
S S YEILGI+A AS+QEIKAAYRRLARV HP+VAA+DRK+SSAD+FMKIH+AYS
Sbjct: 229 FSYSSPGSHYEILGISAGASNQEIKAAYRRLARVCHPDVAAIDRKNSSADDFMKIHSAYS 408
Query: 115 TLSDPEKRASYDRSMNR-RRRPLTAAVTSRFSGYKGRNWETDQCW 158
TLSDP+KRA+YDRS+ R +RRPL+ V+ SGY R WETDQCW
Sbjct: 409 TLSDPDKRANYDRSLFRQQRRPLSTMVS---SGYSSRKWETDQCW 534
>TC77215 similar to PIR|T11855|T11855 DnaJ protein homolog - kidney bean
(fragment), partial (70%)
Length = 858
Score = 158 bits (399), Expect = 1e-39
Identities = 91/165 (55%), Positives = 117/165 (70%), Gaps = 8/165 (4%)
Frame = +3
Query: 2 LSPSFTTATTFSGQIATSPTGHVRSRPVLAFATATE-ADYRPT------PSLHLSTHGDS 54
L S A FSG T P+ V+S+P++ ++AT A+ R T PS + +S
Sbjct: 69 LPSSLYAAGNFSGTSVTPPSCRVKSKPIVITSSATATAEARSTWTEQQRPSY---LNMNS 239
Query: 55 GMASCSSSLYEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYS 114
S +SLY+ILGI+A AS+QEIKAAYRRLARV HP+VAA+DRK+SSAD+FMKIH+AYS
Sbjct: 240 SSHSSPASLYQILGISAGASNQEIKAAYRRLARVCHPDVAAIDRKNSSADDFMKIHSAYS 419
Query: 115 TLSDPEKRASYDRSMNRRR-RPLTAAVTSRFSGYKGRNWETDQCW 158
TLSDP+KRA+YDRS+ R++ RPL+ V+ SGY R WETDQCW
Sbjct: 420 TLSDPDKRANYDRSLFRQQLRPLSTMVS---SGYSSRKWETDQCW 545
>TC83173 similar to PIR|T11855|T11855 DnaJ protein homolog - kidney bean
(fragment), partial (83%)
Length = 675
Score = 156 bits (394), Expect = 4e-39
Identities = 90/163 (55%), Positives = 113/163 (69%), Gaps = 6/163 (3%)
Frame = +1
Query: 2 LSPSFTTATTFSGQIATSPTGHVRSRPVLAFATATE-ADYRPTPSLHLSTHGDSGMASCS 60
L S A FSG T P VRS+P++ A++T A+ R T + M+S S
Sbjct: 1 LPSSLYAAGNFSGTTVTPPPCRVRSKPIITTASSTSTAEPRSTWTTEQQRPSYLNMSSFS 180
Query: 61 SSL----YEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTL 116
S Y+ILGI+AAAS+QEIKAAYRRLAR+ HP+VA+VDRK+SSAD+FMKI AYSTL
Sbjct: 181 HSSPGSHYDILGISAAASNQEIKAAYRRLARLCHPDVASVDRKNSSADDFMKIQVAYSTL 360
Query: 117 SDPEKRASYDRSMN-RRRRPLTAAVTSRFSGYKGRNWETDQCW 158
SDP+KRA+YDRS+ RR+RPL+++ SGY GR WETDQCW
Sbjct: 361 SDPDKRANYDRSLFLRRQRPLSSSAIIS-SGYSGRKWETDQCW 486
>BQ140212 similar to PIR|T11855|T118 DnaJ protein homolog - kidney bean
(fragment), partial (32%)
Length = 317
Score = 100 bits (249), Expect = 2e-22
Identities = 63/114 (55%), Positives = 73/114 (63%), Gaps = 3/114 (2%)
Frame = +3
Query: 1 MLSPSFT---TATTFSGQIATSPTGHVRSRPVLAFATATEADYRPTPSLHLSTHGDSGMA 57
MLS SF + TF+G+ +SP H +SR VL ATA+ +
Sbjct: 30 MLSTSFPAPLSTATFAGKTISSPPFHAKSRRVLISATASTTE-----------------P 158
Query: 58 SCSSSLYEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHA 111
SC+S LYEILG+AA ASDQEIKAAYRRLARV HP+VAAVDRK SSAD MKIHA
Sbjct: 159 SCTS-LYEILGVAAVASDQEIKAAYRRLARVSHPDVAAVDRKVSSADXXMKIHA 317
>TC79039 similar to GP|9294537|dbj|BAB02800.1 DnaJ-like protein {Arabidopsis
thaliana}, partial (30%)
Length = 807
Score = 87.0 bits (214), Expect = 3e-18
Identities = 50/105 (47%), Positives = 65/105 (61%), Gaps = 6/105 (5%)
Frame = +1
Query: 60 SSSLYEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTLSDP 119
++SLYE+L + AS EIK+AYR LA+V HP+ AA ++ + +F++I AY TLSDP
Sbjct: 241 ATSLYEVLRLNPGASAMEIKSAYRSLAKVYHPDAAARRLQECNDGDFIEIRNAYETLSDP 420
Query: 120 EKRASYDRSM---NRRRRPLTAA--VTSRFSG-YKGRNWETDQCW 158
R YD S+ R R TAA + R SG Y R WETDQCW
Sbjct: 421 SSRQIYDLSLMVHGGRNRRFTAAPVMQKRNSGFYTNRRWETDQCW 555
>TC80547 similar to GP|7268087|emb|CAB78425.1 DnaJ-like protein {Arabidopsis
thaliana}, partial (34%)
Length = 776
Score = 70.5 bits (171), Expect = 3e-13
Identities = 33/79 (41%), Positives = 50/79 (62%)
Frame = +2
Query: 53 DSGMASCSSSLYEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAA 112
+ G+ + S Y++LGI + S +IK+AY++LAR HP+V+ DR + F+++ A
Sbjct: 179 NDGVVAEERSFYDLLGIPESGSLMDIKSAYKQLARKYHPDVSPPDRVEEYTKRFIQVQEA 358
Query: 113 YSTLSDPEKRASYDRSMNR 131
Y TLSDP +R YDR M R
Sbjct: 359 YETLSDPSRRIMYDRDMAR 415
>TC89246 homologue to PIR|T06594|T06594 heat shock protein dnaJ - garden
pea, partial (50%)
Length = 1119
Score = 63.9 bits (154), Expect = 3e-11
Identities = 33/70 (47%), Positives = 47/70 (67%)
Frame = +1
Query: 58 SCSSSLYEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTLS 117
+ SS Y LG+ +A+ +EIKAAYRRLAR HP+ V+++ + D+F +I AY LS
Sbjct: 457 AASSDYYSTLGVPKSATGKEIKAAYRRLARQYHPD---VNKEPGATDKFKEISNAYEVLS 627
Query: 118 DPEKRASYDR 127
D +KRA YD+
Sbjct: 628 DDKKRALYDQ 657
>TC86700 similar to GP|18377835|gb|AAL67104.1 At1g24120/F3I6_4 {Arabidopsis
thaliana}, partial (83%)
Length = 1929
Score = 61.2 bits (147), Expect = 2e-10
Identities = 32/65 (49%), Positives = 45/65 (69%)
Frame = +1
Query: 64 YEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTLSDPEKRA 123
YE+LG++ ++DQEIK+AYR+LA HP+ A D K +AD F + +Y+ LSDP+KR
Sbjct: 274 YEVLGVSRNSTDQEIKSAYRKLALKFHPDKNANDPK--AADLFKEATFSYNLLSDPDKRR 447
Query: 124 SYDRS 128
YD S
Sbjct: 448 QYDSS 462
>TC77797 similar to GP|6714354|gb|AAF26045.1| ARG1 protein (Altered Response
to Gravity); 32591-35072 {Arabidopsis thaliana}, partial
(93%)
Length = 2063
Score = 58.9 bits (141), Expect = 8e-10
Identities = 29/63 (46%), Positives = 42/63 (66%)
Frame = +3
Query: 64 YEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTLSDPEKRA 123
YE+LG++ +SDQEIK AYR+LA HP+ A +++ F ++ +Y+ LSDPEKR
Sbjct: 420 YEVLGVSKESSDQEIKTAYRKLALKYHPDKNA--NNPEASELFKEVAYSYNILSDPEKRR 593
Query: 124 SYD 126
YD
Sbjct: 594 QYD 602
>TC77858 similar to GP|20197886|gb|AAD22362.2 putative DnaJ protein
{Arabidopsis thaliana}, partial (78%)
Length = 1869
Score = 58.5 bits (140), Expect = 1e-09
Identities = 37/108 (34%), Positives = 55/108 (50%), Gaps = 10/108 (9%)
Frame = +3
Query: 30 LAFATATEADYRPTPSLHLST---------HGDSGMASCSSSLYEILGIAAAASDQEIKA 80
++F A + + SLH + G + + Y +LG++ ++ EIK
Sbjct: 375 ISFMAAPSSTFFARDSLHFNIGSHQTLHRRRGSRLIVRADADYYTVLGVSKNSTKSEIKT 554
Query: 81 AYRRLARVRHPNVAAVDRKDSSADE-FMKIHAAYSTLSDPEKRASYDR 127
AYR+LAR HP+V KD A+E F +I AY LSD EKR+ YD+
Sbjct: 555 AYRKLARNYHPDV----NKDPGAEEKFKEISNAYEVLSDDEKRSIYDK 686
>AJ503880 similar to GP|12838381|dbj DnaJ (Hsp40) homolog subfamily B
member 6~data source:MGD source key:MGI:1344381
evidence:ISS~, partial (26%)
Length = 466
Score = 58.2 bits (139), Expect = 1e-09
Identities = 32/69 (46%), Positives = 43/69 (61%)
Frame = +1
Query: 59 CSSSLYEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTLSD 118
C+ S YEILGI AS EI+ AYR+ A + HP+ V RK+ + +F KI AY LSD
Sbjct: 31 CTMSYYEILGIEVDASVDEIRKAYRKQALLWHPD-KNVQRKEEAEAKFKKIAEAYEVLSD 207
Query: 119 PEKRASYDR 127
+KR Y++
Sbjct: 208 VDKRQIYNK 234
>BG452391 weakly similar to GP|22135874|gb| DnaJ protein putative
{Arabidopsis thaliana}, partial (59%)
Length = 612
Score = 57.0 bits (136), Expect = 3e-09
Identities = 34/84 (40%), Positives = 45/84 (53%), Gaps = 2/84 (2%)
Frame = +1
Query: 48 LSTHGDSGMASCSSSLYEILGIAAAASDQEIKAAYRRLARVRHPN--VAAVDRKDSSADE 105
L T D S SSS Y ILG+++ +S EI+ AYR+LA HP+ + +
Sbjct: 163 LVTMDDYREGSLSSSYYSILGVSSDSSIDEIRRAYRKLAMQWHPDRWTRTPSLLSEAKCK 342
Query: 106 FMKIHAAYSTLSDPEKRASYDRSM 129
F I AYS LSDP KR+ YD +
Sbjct: 343 FQNIQEAYSVLSDPXKRSMYDAGL 414
>TC85313 PIR|T09338|T09338 DnaJ-like protein MsJ1 - alfalfa, complete
Length = 1927
Score = 56.6 bits (135), Expect = 4e-09
Identities = 28/70 (40%), Positives = 43/70 (61%)
Frame = +1
Query: 58 SCSSSLYEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTLS 117
S ++ Y+ILG++ +AS+ EIK AYR+ A HP+ K ++F ++ AY LS
Sbjct: 307 SDNTKYYDILGVSKSASEDEIKKAYRKAAMKNHPD------KGGDPEKFKELGQAYEVLS 468
Query: 118 DPEKRASYDR 127
DPEK+ YD+
Sbjct: 469 DPEKKELYDQ 498
>TC85315 similar to GP|9758827|dbj|BAB09499.1 gene_id:MTH12.18~unknown
protein {Arabidopsis thaliana}, partial (39%)
Length = 713
Score = 56.6 bits (135), Expect = 4e-09
Identities = 29/70 (41%), Positives = 46/70 (65%)
Frame = +2
Query: 57 ASCSSSLYEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTL 116
A S YE+LG++ +AS EIK AYR+LA HP+ V+++D++ ++F++I AY+TL
Sbjct: 311 AKRGESPYEVLGLSPSASVNEIKKAYRKLALKYHPD---VNKEDNAQEKFLRIKHAYNTL 481
Query: 117 SDPEKRASYD 126
+ R YD
Sbjct: 482 LNSSSRRKYD 511
>TC90060 similar to GP|23428796|gb|AAM23263.1 DnaJ-like protein {Glycine
max}, partial (90%)
Length = 804
Score = 56.2 bits (134), Expect = 5e-09
Identities = 35/82 (42%), Positives = 46/82 (55%), Gaps = 4/82 (4%)
Frame = +1
Query: 52 GDSGMASCSSSLYEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADE----FM 107
G S S SS Y +LGI + AS +I+ AYR+LA HP+ A R ++A E F
Sbjct: 208 GGSNGGSRSSCYYSVLGIRSDASSSDIRTAYRKLAMRWHPDKFA--RNPTTAGEAKRRFQ 381
Query: 108 KIHAAYSTLSDPEKRASYDRSM 129
+I AYS LSD KR+ YD +
Sbjct: 382 QIQEAYSVLSDESKRSMYDAGL 447
>TC87379 similar to PIR|T04618|T04618 heat shock protein homolog F20O9.160 -
Arabidopsis thaliana, partial (75%)
Length = 1431
Score = 56.2 bits (134), Expect = 5e-09
Identities = 30/64 (46%), Positives = 40/64 (61%)
Frame = +2
Query: 64 YEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTLSDPEKRA 123
YEIL + A+D E+K AYR+LA HP+ D K+ + +F I AY LSDP+KRA
Sbjct: 98 YEILEVDKNATDDELKKAYRKLAMKWHPDKNP-DNKNDAETKFKLISEAYEVLSDPQKRA 274
Query: 124 SYDR 127
YD+
Sbjct: 275 IYDQ 286
>TC90074 similar to PIR|G84590|G84590 probable heat shock protein [imported]
- Arabidopsis thaliana, partial (88%)
Length = 1287
Score = 56.2 bits (134), Expect = 5e-09
Identities = 28/64 (43%), Positives = 44/64 (68%)
Frame = +1
Query: 64 YEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTLSDPEKRA 123
Y+IL + A+++E+K AYR+LA HP+ ++KD+ A +F +I AY LSDP+K+A
Sbjct: 16 YKILKVDKNATEEELKKAYRKLAMKWHPDKNPSNKKDAEA-KFKEISEAYEVLSDPQKKA 192
Query: 124 SYDR 127
YD+
Sbjct: 193 IYDQ 204
>TC91543 similar to PIR|H96808|H96808 protein F28K19.14 [imported] -
Arabidopsis thaliana, partial (52%)
Length = 659
Score = 55.8 bits (133), Expect = 7e-09
Identities = 36/106 (33%), Positives = 54/106 (49%), Gaps = 7/106 (6%)
Frame = +2
Query: 41 RPTPSLHLSTHGDSGMASC---SSSLYEILGIAAAASDQEIKAAYRRLARVRHPNV---- 93
RP P L+ A+ + YE L + A D++IK AYRRLA+ HP+V
Sbjct: 221 RPMPQFRLNQKRTVAKATNWDQQKTPYETLELEGDADDEQIKIAYRRLAKFYHPDVYDGR 400
Query: 94 AAVDRKDSSADEFMKIHAAYSTLSDPEKRASYDRSMNRRRRPLTAA 139
++ +++ F+KI AAY L D E+R YD M + P+ A+
Sbjct: 401 GILEEGETAEARFIKIQAAYELLIDSERRKKYD--MANQANPMKAS 532
>TC78760 similar to PIR|T48161|T48161 heat shock protein 40-like -
Arabidopsis thaliana, partial (56%)
Length = 762
Score = 55.5 bits (132), Expect = 9e-09
Identities = 29/64 (45%), Positives = 41/64 (63%)
Frame = +3
Query: 64 YEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTLSDPEKRA 123
Y++L I A D ++K AYR+LA HP+ ++KD+ A +F I AY LSDP+KRA
Sbjct: 30 YKVLQIDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKDAEA-KFKTISEAYDVLSDPQKRA 206
Query: 124 SYDR 127
YD+
Sbjct: 207 VYDQ 218
>TC78759 similar to GP|21618097|gb|AAM67147.1 putative heat shock protein
{Arabidopsis thaliana}, partial (89%)
Length = 1344
Score = 55.5 bits (132), Expect = 9e-09
Identities = 29/64 (45%), Positives = 41/64 (63%)
Frame = +3
Query: 64 YEILGIAAAASDQEIKAAYRRLARVRHPNVAAVDRKDSSADEFMKIHAAYSTLSDPEKRA 123
Y++L I A D ++K AYR+LA HP+ ++KD+ A +F I AY LSDP+KRA
Sbjct: 84 YKVLQIDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKDAEA-KFKTISEAYDVLSDPQKRA 260
Query: 124 SYDR 127
YD+
Sbjct: 261 VYDQ 272
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.125 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,166,299
Number of Sequences: 36976
Number of extensions: 48957
Number of successful extensions: 419
Number of sequences better than 10.0: 95
Number of HSP's better than 10.0 without gapping: 375
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 377
length of query: 158
length of database: 9,014,727
effective HSP length: 88
effective length of query: 70
effective length of database: 5,760,839
effective search space: 403258730
effective search space used: 403258730
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0197.6