BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0195b.8
(83 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE998753 similar to PIR|T45958|T45 oligopeptide transporter-like... 81 8e-17
TC84411 similar to GP|4102839|gb|AAD01600.1| LeOPT1 {Lycopersico... 80 1e-16
TC79810 similar to GP|11994403|dbj|BAB02362. nitrate transporter... 79 3e-16
TC82302 similar to PIR|F84663|F84663 probable nitrate transporte... 72 3e-14
TC88300 similar to GP|9581817|emb|CAC00544.1 putative low-affini... 70 2e-13
TC93408 similar to GP|11933407|dbj|BAB19758. putative nitrate tr... 65 6e-12
BF521028 weakly similar to PIR|E84798|E84 probable peptide/amino... 63 2e-11
TC87099 similar to PIR|G86449|G86449 hypothetical protein AAF813... 61 9e-11
BI308697 similar to PIR|T47604|T476 oligopeptide transporter-lik... 58 7e-10
BE205238 similar to GP|9581817|emb putative low-affinity nitrate... 57 1e-09
AL387494 weakly similar to GP|17381202|gb putative peptide trans... 56 3e-09
BQ147149 similar to PIR|G96720|G967 nitrate transporter (NTL1) ... 55 4e-09
TC79430 similar to PIR|F86358|F86358 Similar to peptide transpor... 54 8e-09
TC79608 similar to GP|20147231|gb|AAM10330.1 At1g68570/F24J5_7 {... 52 3e-08
TC78128 weakly similar to GP|21928025|gb|AAM78041.1 At1g69870/T1... 52 4e-08
TC79437 similar to PIR|G96720|G96720 nitrate transporter (NTL1) ... 52 5e-08
AW684979 similar to GP|15391731|em nitrite transporter {Cucumis ... 51 7e-08
TC78362 similar to GP|15391731|emb|CAA93316. nitrite transporter... 51 7e-08
TC93574 weakly similar to GP|21536862|gb|AAM61194.1 unknown {Ara... 50 1e-07
TC84777 weakly similar to GP|20147231|gb|AAM10330.1 At1g68570/F2... 50 2e-07
>BE998753 similar to PIR|T45958|T45 oligopeptide transporter-like protein -
Arabidopsis thaliana, partial (44%)
Length = 809
Score = 80.9 bits (198), Expect = 8e-17
Identities = 40/63 (63%), Positives = 47/63 (74%), Gaps = 2/63 (3%)
Frame = +3
Query: 21 NPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFG--SFNIP 78
NPWRL TVTQVEELK ++R+LP+WAT I+F+ VY +STL V QG MN G SF IP
Sbjct: 213 NPWRLCTVTQVEELKSILRLLPVWATGIIFATVYGQMSTLFVLQGQTMNTHVGNSSFKIP 392
Query: 79 PAS 81
PAS
Sbjct: 393 PAS 401
>TC84411 similar to GP|4102839|gb|AAD01600.1| LeOPT1 {Lycopersicon
esculentum}, partial (38%)
Length = 804
Score = 80.5 bits (197), Expect = 1e-16
Identities = 39/52 (75%), Positives = 44/52 (84%)
Frame = +1
Query: 30 QVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFGSFNIPPAS 81
QVEELKILIR+ PIWAT I+FS+VYA +STL VEQGTMMN S GSF + PAS
Sbjct: 1 QVEELKILIRMFPIWATGIIFSSVYAQMSTLFVEQGTMMNTSIGSFKLSPAS 156
>TC79810 similar to GP|11994403|dbj|BAB02362. nitrate transporter {Arabidopsis
thaliana}, partial (60%)
Length = 1236
Score = 79.0 bits (193), Expect = 3e-16
Identities = 34/74 (45%), Positives = 54/74 (72%)
Frame = +3
Query: 8 LVSDSESRSGDYANPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTM 67
++ +++S+ G+ NPW + T+TQVEE+K++I++LPIW+T I+F VY+ ++T +EQ T
Sbjct: 960 ILDETKSKDGNNENPWLVSTMTQVEEVKMVIKLLPIWSTCILFWTVYSQMNTFTIEQATF 1139
Query: 68 MNRSFGSFNIPPAS 81
MNR GS IP S
Sbjct: 1140 MNRKVGSLEIPAGS 1181
>TC82302 similar to PIR|F84663|F84663 probable nitrate transporter
[imported] - Arabidopsis thaliana, partial (51%)
Length = 1133
Score = 72.4 bits (176), Expect = 3e-14
Identities = 32/65 (49%), Positives = 45/65 (69%)
Frame = +3
Query: 17 GDYANPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFGSFN 76
G NPW+L ++T+VEE+K+++R+LPIWAT I+F YA + T VEQ M R+ G+F
Sbjct: 177 GSGPNPWKLCSLTRVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQAATMERNVGNFQ 356
Query: 77 IPPAS 81
IP S
Sbjct: 357 IPAGS 371
>TC88300 similar to GP|9581817|emb|CAC00544.1 putative low-affinity nitrate
transporter {Nicotiana plumbaginifolia}, partial (56%)
Length = 1169
Score = 69.7 bits (169), Expect = 2e-13
Identities = 34/60 (56%), Positives = 44/60 (72%), Gaps = 1/60 (1%)
Frame = +1
Query: 23 WRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFG-SFNIPPAS 81
W L T+T VEE+K+++R+LPIWAT I+F VYA ++T V Q T +NR G SF IPPAS
Sbjct: 286 WYLSTLTDVEEVKLVLRMLPIWATTIMFWTVYAQMTTFSVSQATTLNRHIGKSFQIPPAS 465
>TC93408 similar to GP|11933407|dbj|BAB19758. putative nitrate transporter
NRT1-3 {Glycine max}, partial (44%)
Length = 909
Score = 64.7 bits (156), Expect = 6e-12
Identities = 33/71 (46%), Positives = 44/71 (61%)
Frame = +1
Query: 11 DSESRSGDYANPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNR 70
D S +PW L TVT VEE K ++R++PI V S + A ++TL V+QGT ++R
Sbjct: 304 DKASVKTGSTSPWMLCTVTHVEETKQMLRMIPILVATFVPSTMMAQVNTLFVKQGTTLDR 483
Query: 71 SFGSFNIPPAS 81
GSF IPPAS
Sbjct: 484 HIGSFKIPPAS 516
>BF521028 weakly similar to PIR|E84798|E84 probable peptide/amino acid
transporter [imported] - Arabidopsis thaliana, partial
(24%)
Length = 539
Score = 63.2 bits (152), Expect = 2e-11
Identities = 32/62 (51%), Positives = 39/62 (62%), Gaps = 1/62 (1%)
Frame = +3
Query: 21 NPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFG-SFNIPP 79
NPWRL TVT+VEE K+++ V+PIW T + A STL V+Q MN SF IPP
Sbjct: 318 NPWRLATVTRVEETKLILNVIPIWLTSLTTGICVAQGSTLFVKQAASMNLKISDSFTIPP 497
Query: 80 AS 81
AS
Sbjct: 498 AS 503
>TC87099 similar to PIR|G86449|G86449 hypothetical protein AAF81343.1
[imported] - Arabidopsis thaliana, partial (86%)
Length = 2205
Score = 60.8 bits (146), Expect = 9e-11
Identities = 26/55 (47%), Positives = 38/55 (68%)
Frame = +3
Query: 11 DSESRSGDYANPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQG 65
D E + G NPW L +TQVEE+K ++R+LPIW I++S V+ +++L VEQG
Sbjct: 1044 DLEVQKGGQHNPWXLCPITQVEEVKCILRLLPIWLCTIIYSVVFTQMASLFVEQG 1208
>BI308697 similar to PIR|T47604|T476 oligopeptide transporter-like protein -
Arabidopsis thaliana, partial (12%)
Length = 423
Score = 57.8 bits (138), Expect = 7e-10
Identities = 25/53 (47%), Positives = 39/53 (73%)
Frame = +2
Query: 21 NPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFG 73
NPWRL +VTQVEE+K+++R++PIW + I+F+ V A L T ++QG + + G
Sbjct: 263 NPWRLCSVTQVEEVKLVVRLIPIWLSCIMFTVVQAQLHTYFLKQGGTLIHTLG 421
>BE205238 similar to GP|9581817|emb putative low-affinity nitrate transporter
{Nicotiana plumbaginifolia}, partial (27%)
Length = 507
Score = 57.0 bits (136), Expect = 1e-09
Identities = 25/53 (47%), Positives = 38/53 (71%)
Frame = +3
Query: 23 WRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFGSF 75
W + T+T VEE+K++IR+LPIWAT I+F ++A ++T V Q T M+ G+F
Sbjct: 345 WYISTLTDVEEVKMVIRMLPIWATTIMFWTIHAQMTTFSVSQATTMDCQIGTF 503
>AL387494 weakly similar to GP|17381202|gb putative peptide transporter
protein {Arabidopsis thaliana}, partial (25%)
Length = 495
Score = 55.8 bits (133), Expect = 3e-09
Identities = 29/71 (40%), Positives = 43/71 (59%), Gaps = 1/71 (1%)
Frame = +2
Query: 11 DSESRSGDYANPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNR 70
D + +PW L TVTQVEE K + +++PI T ++ S + +TL V+QGT ++R
Sbjct: 50 DKAATKTGQTSPWMLCTVTQVEETKQMTKMIPILITTLIPSTLLVQSTTLFVKQGTTLDR 229
Query: 71 SFG-SFNIPPA 80
G F+IPPA
Sbjct: 230 RMGPHFDIPPA 262
>BQ147149 similar to PIR|G96720|G967 nitrate transporter (NTL1) 53025-56402
[imported] - Arabidopsis thaliana, partial (14%)
Length = 692
Score = 55.5 bits (132), Expect = 4e-09
Identities = 25/55 (45%), Positives = 38/55 (68%)
Frame = +2
Query: 27 TVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFGSFNIPPAS 81
T+ +VEE KI++++LPI+ + I+ + A LST V+Q + MN GSF +PPAS
Sbjct: 449 TIKEVEEAKIVLKILPIFMSTIMLNCCLAQLSTFSVQQSSTMNTMIGSFRVPPAS 613
>TC79430 similar to PIR|F86358|F86358 Similar to peptide transporter
[imported] - Arabidopsis thaliana, partial (55%)
Length = 1303
Score = 54.3 bits (129), Expect = 8e-09
Identities = 26/77 (33%), Positives = 47/77 (60%), Gaps = 1/77 (1%)
Frame = +2
Query: 8 LVSDSESRSGDYANPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTM 67
+ SD +G ++ +V++VEE K ++R++PIWAT ++F+ V++ ST +QG
Sbjct: 890 IASDGSKENG------KVCSVSEVEEAKAILRLVPIWATSLIFAIVFSQSSTFFTKQGVT 1051
Query: 68 MNRS-FGSFNIPPASCE 83
++R F +PPAS +
Sbjct: 1052LDRKILPGFYVPPASLQ 1102
>TC79608 similar to GP|20147231|gb|AAM10330.1 At1g68570/F24J5_7 {Arabidopsis
thaliana}, partial (59%)
Length = 1489
Score = 52.4 bits (124), Expect = 3e-08
Identities = 29/60 (48%), Positives = 36/60 (59%), Gaps = 1/60 (1%)
Frame = +2
Query: 23 WRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSF-GSFNIPPAS 81
WRL TV +VEELK +IR+ PIWA+ I+ YA T ++Q MNR SF IP S
Sbjct: 299 WRLSTVHRVEELKSIIRMGPIWASGILLITAYAQQGTFSLQQAKTMNRHLTKSFEIPAGS 478
>TC78128 weakly similar to GP|21928025|gb|AAM78041.1 At1g69870/T17F3_10
{Arabidopsis thaliana}, partial (77%)
Length = 2022
Score = 52.0 bits (123), Expect = 4e-08
Identities = 28/66 (42%), Positives = 34/66 (51%), Gaps = 1/66 (1%)
Frame = +2
Query: 17 GDYANPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFG-SF 75
G N W L ++ QVEE+K L R LPIWA I+ A T +V Q M+R G F
Sbjct: 1109 GTIVNQWNLVSIQQVEEVKCLARTLPIWAAGILGFTAMAQQGTFIVSQAMKMDRHLGPKF 1288
Query: 76 NIPPAS 81
IP S
Sbjct: 1289 QIPAGS 1306
>TC79437 similar to PIR|G96720|G96720 nitrate transporter (NTL1) 53025-56402
[imported] - Arabidopsis thaliana, partial (61%)
Length = 1424
Score = 51.6 bits (122), Expect = 5e-08
Identities = 25/56 (44%), Positives = 38/56 (67%), Gaps = 1/56 (1%)
Frame = +1
Query: 27 TVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRS-FGSFNIPPAS 81
T+ Q+E++K++ ++ PI+A I+ +A A LST VEQ MN + F SF +PPAS
Sbjct: 1018 TIQQLEDVKLVFKIFPIFACTIMLNACLAQLSTFSVEQAATMNTTLFSSFKVPPAS 1185
>AW684979 similar to GP|15391731|em nitrite transporter {Cucumis sativus},
partial (23%)
Length = 455
Score = 51.2 bits (121), Expect = 7e-08
Identities = 28/76 (36%), Positives = 47/76 (61%), Gaps = 2/76 (2%)
Frame = +3
Query: 8 LVSDSESRSGDYA-NPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGT 66
++++ E++ + N W L TV +VEELK +IR+LPIWA+ I+ + L + +++Q
Sbjct: 171 IITEEEAKDSNAPPNLWNLVTVHRVEELKAIIRMLPIWASGILLIMSSSHLGSFVIQQAR 350
Query: 67 MMNRSF-GSFNIPPAS 81
M+R SF I PA+
Sbjct: 351 SMDRHLTHSFQIAPAN 398
>TC78362 similar to GP|15391731|emb|CAA93316. nitrite transporter {Cucumis
sativus}, partial (88%)
Length = 1871
Score = 51.2 bits (121), Expect = 7e-08
Identities = 29/76 (38%), Positives = 45/76 (59%), Gaps = 2/76 (2%)
Frame = +3
Query: 8 LVSDSESRS-GDYANPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGT 66
+V+D E+++ N W L TV +VEELK L+R+LPIWA+ I+ + + ++ Q
Sbjct: 915 IVTDEEAKNLNKLPNLWNLATVHRVEELKCLVRMLPIWASGILLITASSSQHSFVIVQAR 1094
Query: 67 MMNRSFG-SFNIPPAS 81
M+R +F I PAS
Sbjct: 1095TMDRHLSHTFEISPAS 1142
>TC93574 weakly similar to GP|21536862|gb|AAM61194.1 unknown {Arabidopsis
thaliana}, partial (35%)
Length = 980
Score = 50.4 bits (119), Expect = 1e-07
Identities = 29/57 (50%), Positives = 35/57 (60%)
Frame = +1
Query: 25 LFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFGSFNIPPAS 81
L TV QVEELK +I+VLP+W+T I AV + V Q T MNR +F IPP S
Sbjct: 751 LCTVRQVEELKAVIQVLPLWSTGITI-AVTISQQSFSVVQATTMNRMVHNFEIPPTS 918
>TC84777 weakly similar to GP|20147231|gb|AAM10330.1 At1g68570/F24J5_7
{Arabidopsis thaliana}, partial (29%)
Length = 684
Score = 50.1 bits (118), Expect = 2e-07
Identities = 28/63 (44%), Positives = 41/63 (64%), Gaps = 1/63 (1%)
Frame = +3
Query: 20 ANPWRLFTVTQVEELKILIRVLPIWATEIVFSAVYALLSTLLVEQGTMMNRSFG-SFNIP 78
+N W+L TV +VEELK +IR+LPI A+ I+ A A L + ++EQ M+R +F I
Sbjct: 102 SNFWKLATVHRVEELKSIIRILPISASGILLIAASAHLPSFVIEQARTMDRHLSHTFQIS 281
Query: 79 PAS 81
PA+
Sbjct: 282 PAN 290
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.134 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,404,032
Number of Sequences: 36976
Number of extensions: 23734
Number of successful extensions: 145
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of query: 83
length of database: 9,014,727
effective HSP length: 59
effective length of query: 24
effective length of database: 6,833,143
effective search space: 163995432
effective search space used: 163995432
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0195b.8