BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0189.9
(214 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG586308 weakly similar to PIR|F84528|F8 probable retroelement p... 70 1e-21
TC77595 weakly similar to PIR|T18350|T18350 probable pol polypro... 56 1e-08
BG454871 weakly similar to GP|10140673|g putative gag-pol polypr... 46 1e-05
BF647164 weakly similar to GP|6691191|gb F7F22.15 {Arabidopsis t... 39 0.001
TC93066 weakly similar to GP|19920130|gb|AAM08562.1 Putative ret... 38 0.003
BF631997 weakly similar to GP|18542925|gb Putative pol polyprote... 32 0.14
TC83351 similar to PIR|T47788|T47788 hypothetical protein F17J16... 31 0.31
TC84633 similar to PIR|T49099|T49099 dihydrolipoamide S-acetyltr... 30 0.91
TC79495 similar to SP|P09886|HS2C_PEA Small heat shock protein ... 28 2.6
TC88617 similar to GP|10177610|dbj|BAB10957. UDP-glucuronyltrans... 28 2.6
TC79494 similar to SP|P09886|HS2C_PEA Small heat shock protein ... 28 2.6
BF636649 similar to GP|21628724|emb OSJNBa0033H08.7 {Oryza sativ... 27 4.5
AL366209 similar to PIR|D86142|D8 hypothetical protein AAF97333.... 27 5.9
TC85617 similar to GP|2369766|emb|CAA04664.1 hypothetical protei... 27 5.9
TC87755 similar to PIR|S52657|S52657 seed biotin-containing prot... 27 5.9
TC84361 similar to PIR|C86247|C86247 hypothetical protein [impor... 27 7.7
TC80683 homologue to GP|8777424|dbj|BAA97014.1 gb|AAF56406.1~gen... 27 7.7
BF003590 27 7.7
>BG586308 weakly similar to PIR|F84528|F8 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(7%)
Length = 686
Score = 70.1 bits (170), Expect(2) = 1e-21
Identities = 32/64 (50%), Positives = 44/64 (68%)
Frame = -2
Query: 1 VSDNGMQFASNQTKEFCEEMGIQRRFSSVEHPQTNGQADSANKVIQQGLKRPLSEAKGAW 60
V+DNG F SN+ +EFCE I+ +S +PQ+NGQA+++NK+I GLK+ L KG W
Sbjct: 664 VTDNGSHFISNKFREFCERWRIRLNTASPRYPQSNGQAEASNKIIIDGLKKRLDLKKGCW 485
Query: 61 LDEL 64
DEL
Sbjct: 484 ADEL 473
Score = 49.7 bits (117), Expect(2) = 1e-21
Identities = 32/119 (26%), Positives = 55/119 (45%), Gaps = 6/119 (5%)
Frame = -3
Query: 67 VFWSYNTTQHSTTGKTPFRMTYGADVMLPVEIDNSSW--RTTPKFEGENSSNMAVELDLL 124
V WS+ T T TPF M + + M P E++ +S P+ N+ + L+ +
Sbjct: 465 VLWSHRTNPRGATKSTPFSMAHRVEAMAPAEVNVTSL*RSRMPQNIELNNDRLFNALETI 286
Query: 125 SETHNEARLKEAAMKQRAAAKYDTKVKPREMQEGDLVLKK----RTGVTGNKLSPIWEG 179
E ++A L+ + + + Y+ VK + ++ GD+VL K + KL WEG
Sbjct: 285 EERRDQALLRIQNYQHQIESYYNKTVKSQPLKLGDIVLCKVFENTKELNAGKLGTNWEG 109
>TC77595 weakly similar to PIR|T18350|T18350 probable pol polyprotein - rice
blast fungus gypsy retroelement (fragment), partial
(14%)
Length = 1708
Score = 55.8 bits (133), Expect = 1e-08
Identities = 43/162 (26%), Positives = 78/162 (47%), Gaps = 1/162 (0%)
Frame = +2
Query: 1 VSDNGMQFASNQTKEFCEEMGIQRRFSSVEHPQTNGQADSANKVIQQGLKRPLSEAKGAW 60
VSD G + +EFC G+ + S+ HPQT+G + N+ IQ L+ + ++ W
Sbjct: 581 VSDRGSNWVGRFWREFCRLTGVTQLLSTSYHPQTDGGTERWNQEIQAVLRAYVCWSQDNW 760
Query: 61 LDELPIVFWSYNTTQHSTTGKTPFRMTYGADVMLPVEIDNSSWRTTPKFEGENSSNMAVE 120
D LP V + +S+ G TPF + +G V ++++ + EGE ++ + V+
Sbjct: 761 GDLLPTVQLALRNRHNSSIGATPFFVEHGYHVDPIPTVEDTGGVVS---EGEAAAQLLVK 931
Query: 121 LDLLSETHNEARLKEAAMKQRAAAKYDTKVKPRE-MQEGDLV 161
+ + + + A +QR+ A + + P + Q GD V
Sbjct: 932 --RMKDVTGFIQAEIVAAQQRSEASANKRRCPADRYQVGDKV 1051
>BG454871 weakly similar to GP|10140673|g putative gag-pol polyprotein {Oryza
sativa (japonica cultivar-group)}, partial (7%)
Length = 674
Score = 45.8 bits (107), Expect = 1e-05
Identities = 32/117 (27%), Positives = 54/117 (45%)
Frame = +2
Query: 10 SNQTKEFCEEMGIQRRFSSVEHPQTNGQADSANKVIQQGLKRPLSEAKGAWLDELPIVFW 69
SN K+ + G SS HP ++GQ+++ NK + L+ + W P +
Sbjct: 32 SNFWKQLFKLHGTILTMSSAYHP*SDGQSEALNKGXEMYLRCLMFTDPLKWSKAFPWAEY 211
Query: 70 SYNTTQHSTTGKTPFRMTYGADVMLPVEIDNSSWRTTPKFEGENSSNMAVELDLLSE 126
YNT+ + + TPF+ YG D+ + + SS K + S +A +LLS+
Sbjct: 212 WYNTSYNISAAMTPFKALYGRDLSMLIRSKGSS-----KDTADLQSQLAQREELLSQ 367
>BF647164 weakly similar to GP|6691191|gb F7F22.15 {Arabidopsis thaliana},
partial (6%)
Length = 469
Score = 38.9 bits (89), Expect = 0.001
Identities = 24/80 (30%), Positives = 39/80 (48%), Gaps = 2/80 (2%)
Frame = +1
Query: 119 VELDLLSETHNEARLKEAAMKQRAAAKYDTKVKPREMQEGDLVL--KKRTGVTGNKLSPI 176
+EL+ L E +A K+R +D ++ RE +EG+LVL R + KL
Sbjct: 100 LELNELEELRLDAYENAKIYKERTKKWHDRRIIRREFREGELVLLFNSRLKLFPGKLRSH 279
Query: 177 WEGPYRILKALGRGAYHLES 196
W GP+++ + GA + S
Sbjct: 280 WSGPFQVKNVMPSGAVEVWS 339
>TC93066 weakly similar to GP|19920130|gb|AAM08562.1 Putative retroelement
{Oryza sativa} [Oryza sativa (japonica cultivar-group)],
partial (10%)
Length = 823
Score = 38.1 bits (87), Expect = 0.003
Identities = 22/82 (26%), Positives = 38/82 (45%), Gaps = 5/82 (6%)
Frame = +1
Query: 1 VSDNGMQFASNQTKEFCEEMGIQRRFSSVEHPQTNGQADSANKVIQQGLKRPLSEA---- 56
++DN ++F S+ EFC GI R + +PQ NG A+ + + + + LS A
Sbjct: 298 ITDN*LEFCSSDFNEFCTNHGIARHKTIPRNPQQNGVAERMIRTLLERARCMLSNAGL*N 477
Query: 57 -KGAWLDELPIVFWSYNTTQHS 77
+ W++ N + HS
Sbjct: 478 *RDLWVEAASTACHLVNRSPHS 543
>BF631997 weakly similar to GP|18542925|gb Putative pol polyprotein {Oryza
sativa}, partial (6%)
Length = 650
Score = 32.3 bits (72), Expect = 0.14
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = -2
Query: 3 DNGMQFASNQTKEFCEEMGIQRRFSSVEHPQTNGQADSAN 42
+NG++ S + E C+E I R+++ PQ NG A+ N
Sbjct: 544 NNGLEICSAEFNELCKEEHITRQYTVRNTPQKNGVAERMN 425
>TC83351 similar to PIR|T47788|T47788 hypothetical protein F17J16.110 -
Arabidopsis thaliana, partial (18%)
Length = 882
Score = 31.2 bits (69), Expect = 0.31
Identities = 20/82 (24%), Positives = 36/82 (43%), Gaps = 1/82 (1%)
Frame = +1
Query: 97 EIDNSSWRTTPKFEGENSSNMAVELDLLSETHNEARLKEAAMKQRAAAKYDTKV-KPREM 155
E+ + + T + G N A E+ LSE R+ E + + +K KP +
Sbjct: 13 ELKSGVRKKTSQRSGSARRNRAAEVHNLSERRRRDRINEKMKALQQLIPHSSKTDKPSML 192
Query: 156 QEGDLVLKKRTGVTGNKLSPIW 177
+EG+ +L+ + T + IW
Sbjct: 193 EEGN*ILEITSVTTSGNVDGIW 258
>TC84633 similar to PIR|T49099|T49099 dihydrolipoamide S-acetyltransferase
precursor - Arabidopsis thaliana, partial (35%)
Length = 928
Score = 29.6 bits (65), Expect = 0.91
Identities = 20/77 (25%), Positives = 34/77 (43%)
Frame = +3
Query: 123 LLSETHNEARLKEAAMKQRAAAKYDTKVKPREMQEGDLVLKKRTGVTGNKLSPIWEGPYR 182
L + H + +K+ AA + K+KP+E Q G + +K I P
Sbjct: 309 LKNADHKTISAISSEVKELAAKAREGKLKPQEFQGGTFSISNLGMYPVDKFCAIINPPQA 488
Query: 183 ILKALGRGAYHLESLDG 199
+ A+GRG +E++ G
Sbjct: 489 CILAVGRGNKVVEAVIG 539
>TC79495 similar to SP|P09886|HS2C_PEA Small heat shock protein chloroplast
precursor. [Garden pea] {Pisum sativum}, partial (54%)
Length = 494
Score = 28.1 bits (61), Expect = 2.6
Identities = 21/84 (25%), Positives = 43/84 (51%), Gaps = 8/84 (9%)
Frame = +3
Query: 86 MTYGA-DVMLPVEIDNSSWRTTPKFE----GENSSNMAVELDLL---SETHNEARLKEAA 137
MT+G ++ +P +I + +F+ + ++VE D+L S+ H E +E +
Sbjct: 231 MTFGGGEIRVPWDIKDEENEIKMRFDMPGLSKEDVKVSVENDVLVIKSDMHKEESGEEDS 410
Query: 138 MKQRAAAKYDTKVKPREMQEGDLV 161
+++ + YDT++K + E D V
Sbjct: 411 WSRKSYSSYDTRLKLPDNCEKDKV 482
>TC88617 similar to GP|10177610|dbj|BAB10957. UDP-glucuronyltransferase-like
protein {Arabidopsis thaliana}, partial (75%)
Length = 1958
Score = 28.1 bits (61), Expect = 2.6
Identities = 19/75 (25%), Positives = 29/75 (38%), Gaps = 2/75 (2%)
Frame = +1
Query: 78 TTGKTPFRMTYGADVMLPVEIDNSSWRTTPKF--EGENSSNMAVELDLLSETHNEARLKE 135
T K P R + + V + W +P EN A ++ S TH
Sbjct: 1369 TDSKFPARWVIDPPLDITVPSKRTPWPDSPPELPSNENEKVFAAGAEVHSNTHTTKTKTP 1548
Query: 136 AAMKQRAAAKYDTKV 150
+ + R+ K+DTKV
Sbjct: 1549 RSRRSRSKRKHDTKV 1593
>TC79494 similar to SP|P09886|HS2C_PEA Small heat shock protein chloroplast
precursor. [Garden pea] {Pisum sativum}, partial (86%)
Length = 901
Score = 28.1 bits (61), Expect = 2.6
Identities = 21/84 (25%), Positives = 43/84 (51%), Gaps = 8/84 (9%)
Frame = +1
Query: 86 MTYGA-DVMLPVEIDNSSWRTTPKFE----GENSSNMAVELDLL---SETHNEARLKEAA 137
MT+G ++ +P +I + +F+ + ++VE D+L S+ H E +E +
Sbjct: 454 MTFGGGEIRVPWDIKDEENEIKMRFDMPGLSKEDVKVSVENDVLVIKSDMHKEESGEEDS 633
Query: 138 MKQRAAAKYDTKVKPREMQEGDLV 161
+++ + YDT++K + E D V
Sbjct: 634 WSRKSYSSYDTRLKLPDNCEKDKV 705
>BF636649 similar to GP|21628724|emb OSJNBa0033H08.7 {Oryza sativa},
partial (4%)
Length = 653
Score = 27.3 bits (59), Expect = 4.5
Identities = 18/52 (34%), Positives = 23/52 (43%)
Frame = +1
Query: 37 QADSANKVIQQGLKRPLSEAKGAWLDELPIVFWSYNTTQHSTTGKTPFRMTY 88
QA N ++ L SE +G L YNT H T G TPF++ Y
Sbjct: 16 QAGLLNHTLETHLLYFTSEQQGV*NFFLTWAECLYNTNFHRTAGCTPFKVVY 171
>AL366209 similar to PIR|D86142|D8 hypothetical protein AAF97333.1 [imported]
- Arabidopsis thaliana, partial (10%)
Length = 455
Score = 26.9 bits (58), Expect = 5.9
Identities = 33/116 (28%), Positives = 45/116 (38%), Gaps = 5/116 (4%)
Frame = +1
Query: 9 ASNQTKEFCEEMGIQRRFSSVEHPQTNGQADSANKVIQQGLKRPLSEA-KGAWLDELPIV 67
A N K CE +EH + AD ++ G K LSE+ DE I
Sbjct: 157 ACNDEKTACE----------LEHKVWDAVADETASAVRYGFKEHLSESPSSVSCDEQKI- 303
Query: 68 FWSYNTTQHSTTGKTPFRMTYGADVMLPVEID-NSSWRTTPKFEGENSS---NMAV 119
N+ + K PF + V LPV +D W TP + E + NMA+
Sbjct: 304 ----NSHDNGRIHK-PFHLRQ-VKVELPVRVDFVGGWSDTPPWSIERAGCVLNMAI 453
>TC85617 similar to GP|2369766|emb|CAA04664.1 hypothetical protein {Citrus x
paradisi}, partial (85%)
Length = 1489
Score = 26.9 bits (58), Expect = 5.9
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = -2
Query: 66 IVFWSYNTTQHSTTGKTP 83
I FWS N TQHS TP
Sbjct: 1455 IQFWSRNKTQHSINNHTP 1402
>TC87755 similar to PIR|S52657|S52657 seed biotin-containing protein SPB65
[validated] - garden pea, partial (52%)
Length = 1368
Score = 26.9 bits (58), Expect = 5.9
Identities = 16/74 (21%), Positives = 33/74 (43%)
Frame = +2
Query: 98 IDNSSWRTTPKFEGENSSNMAVELDLLSETHNEARLKEAAMKQRAAAKYDTKVKPREMQE 157
++ ++ PK G ++ V L + A+ A K + +Y+TK ++QE
Sbjct: 374 VEGAAGYVVPKAAGLALKSVDVVKGLAASAGETAKEFTAKKKDESWREYETKRASGQLQE 553
Query: 158 GDLVLKKRTGVTGN 171
G+ ++ G+ N
Sbjct: 554 GEEIMPSSGGIGEN 595
>TC84361 similar to PIR|C86247|C86247 hypothetical protein [imported] -
Arabidopsis thaliana, partial (13%)
Length = 761
Score = 26.6 bits (57), Expect = 7.7
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -1
Query: 45 IQQGLKRPLSEAKGAWLDELPIVFWS 70
I L+RP E+K +WLD + WS
Sbjct: 206 ISVDLRRPKMESKKSWLDSAKALNWS 129
>TC80683 homologue to GP|8777424|dbj|BAA97014.1
gb|AAF56406.1~gene_id:K9P8.7~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (16%)
Length = 1360
Score = 26.6 bits (57), Expect = 7.7
Identities = 25/101 (24%), Positives = 45/101 (43%)
Frame = +1
Query: 104 RTTPKFEGENSSNMAVELDLLSETHNEARLKEAAMKQRAAAKYDTKVKPREMQEGDLVLK 163
++ +E EN+ +LDL SE HNE+ K A + +K + + E + G+
Sbjct: 622 KSNKSYEAENT-----QLDLSSENHNESS-KPAVRDKPYISKAERRKLKNEPKHGEAHPS 783
Query: 164 KRTGVTGNKLSPIWEGPYRILKALGRGAYHLESLDGKRVPR 204
G +KL I + + A +L++ GK++ R
Sbjct: 784 DGNGKDKSKLKDISGDLH------AKDAENLKTGGGKKISR 888
>BF003590
Length = 531
Score = 26.6 bits (57), Expect = 7.7
Identities = 14/43 (32%), Positives = 23/43 (52%), Gaps = 5/43 (11%)
Frame = -1
Query: 92 VMLPVEIDNSSWRTTPKFEGENSSN-----MAVELDLLSETHN 129
+ + ++I ++ WR TPKF + N M ++LLSE N
Sbjct: 531 IHIQIQIHHTWWRGTPKFSIRSHCNCSNCRMV*TIELLSELQN 403
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.131 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,620,614
Number of Sequences: 36976
Number of extensions: 62462
Number of successful extensions: 260
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 258
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 260
length of query: 214
length of database: 9,014,727
effective HSP length: 92
effective length of query: 122
effective length of database: 5,612,935
effective search space: 684778070
effective search space used: 684778070
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0189.9