BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0181.4
(987 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF649369 46 8e-05
AL376506 homologue to GP|15021744|gb root nodule extensin {Pisum... 40 0.004
TC76689 MtN12 39 0.008
TC76718 homologue to GP|8132441|gb|AAF73291.1| extensin {Pisum s... 39 0.010
BF642399 similar to PIR|T10863|T10 extensin precursor - kidney b... 39 0.010
BF639374 similar to GP|8132441|gb| extensin {Pisum sativum}, par... 38 0.017
BQ141029 similar to GP|8132441|gb| extensin {Pisum sativum}, par... 36 0.084
TC92009 35 0.14
BE998536 similar to GP|2598593|emb| MtN12 {Medicago truncatula},... 35 0.14
TC86164 weakly similar to GP|20514261|gb|AAM22959.1 tubulin fold... 35 0.14
TC82241 similar to PIR|B84620|B84620 hypothetical protein At2g23... 34 0.24
BE325851 similar to PIR|T02573|T025 CDP-diacylglycerol--glycerol... 33 0.54
TC76721 homologue to GP|8132441|gb|AAF73291.1| extensin {Pisum s... 33 0.71
TC93091 similar to GP|4115538|dbj|BAA36412.1 UDP-glycose:flavono... 32 0.92
TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein... 32 0.92
BQ144271 weakly similar to GP|21322711|em pherophorin-dz1 protei... 32 1.2
AL368332 weakly similar to GP|21322711|e pherophorin-dz1 protein... 32 1.2
TC81780 homologue to GP|20521948|dbj|BAB13408. KIAA1582 protein ... 32 1.2
TC76431 similar to GP|8132441|gb|AAF73291.1| extensin {Pisum sat... 32 1.6
BQ141680 similar to GP|11993889|gb| virion-associated nuclear-sh... 31 2.1
>BF649369
Length = 631
Score = 45.8 bits (107), Expect = 8e-05
Identities = 27/90 (30%), Positives = 42/90 (46%), Gaps = 2/90 (2%)
Frame = +3
Query: 79 SQTQHSALSSPPLKLIKFSGSDPTFWLLNTEVFFLQHPWPLELRFQFIALYLEGQALTWF 138
S S L+ +KL F G DP W+ E++F P ++R + L +EG + WF
Sbjct: 90 SSQNESRLAGKKVKLPLFEGDDPVAWITRAEIYFDVQNTPDDMRVKLSRLSMEGPTIHWF 269
Query: 139 NLWRHQLD--SWEKFREVFKLQFIQFRSEH 166
NL D S EK ++ ++ R E+
Sbjct: 270 NLLMETEDDLSREKLKKALIARYDGRRLEN 359
>AL376506 homologue to GP|15021744|gb root nodule extensin {Pisum sativum},
partial (68%)
Length = 470
Score = 40.0 bits (92), Expect = 0.004
Identities = 23/68 (33%), Positives = 28/68 (40%), Gaps = 6/68 (8%)
Frame = +2
Query: 58 SIPPQPP------PIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDPTFWLLNTEVF 111
S PP PP P+YH P P H YP H SPP + K+ P + V
Sbjct: 128 SSPPPPPVHTYPKPVYHSPPPPVHTYPKPVYH----SPPPPVHKYPHPKPVYHSPPPPVH 295
Query: 112 FLQHPWPL 119
HP P+
Sbjct: 296 KYPHPHPV 319
Score = 35.4 bits (80), Expect = 0.11
Identities = 16/38 (42%), Positives = 18/38 (47%), Gaps = 2/38 (5%)
Frame = +2
Query: 61 PQPPPIYHHNPAPYHNY--PSQTQHSALSSPPLKLIKF 96
P P P+YH P P H Y P HS PP K K+
Sbjct: 302 PHPHPVYHSPPPPVHKYPHPHPVYHSPPPPPPKKSYKY 415
>TC76689 MtN12
Length = 759
Score = 39.3 bits (90), Expect = 0.008
Identities = 14/30 (46%), Positives = 15/30 (49%)
Frame = +1
Query: 61 PQPPPIYHHNPAPYHNYPSQTQHSALSSPP 90
P P P+YH P P H YP H SPP
Sbjct: 250 PHPKPVYHSPPPPVHTYPPHVPHPVYHSPP 339
Score = 35.0 bits (79), Expect = 0.14
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = +1
Query: 61 PQPPPIYHHNPAPYHNYPSQTQHS 84
P P P+YH P P H YP HS
Sbjct: 151 PHPKPVYHSPPPPVHTYPKPVYHS 222
>TC76718 homologue to GP|8132441|gb|AAF73291.1| extensin {Pisum sativum},
partial (63%)
Length = 712
Score = 38.9 bits (89), Expect = 0.010
Identities = 21/65 (32%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
Frame = +1
Query: 39 ISAISGLIDQVSFLNGHLRSIPPQPP-PIYHHNP-APYHNYPSQTQHSALSSPPLKLIKF 96
++ IS + + N ++ S PP PP P Y+H+P P H+ P +S+ PP K K+
Sbjct: 70 LAIISLTLPSQTSANSYIYSSPPPPPKPYYYHSPPPPVHSPPPPYHYSSPPPPPKKPYKY 249
Query: 97 SGSDP 101
+ P
Sbjct: 250 ASPPP 264
>BF642399 similar to PIR|T10863|T10 extensin precursor - kidney bean, partial
(33%)
Length = 653
Score = 38.9 bits (89), Expect = 0.010
Identities = 21/65 (32%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
Frame = +3
Query: 39 ISAISGLIDQVSFLNGHLRSIPPQPP-PIYHHNP-APYHNYPSQTQHSALSSPPLKLIKF 96
++ IS + + N ++ S PP PP P Y+H+P P H+ P +S+ PP K K+
Sbjct: 45 LAIISLTLPSQTSANSYIYSSPPPPPKPYYYHSPPPPVHSPPPPYHYSSPPPPPKKPYKY 224
Query: 97 SGSDP 101
+ P
Sbjct: 225 ASPPP 239
>BF639374 similar to GP|8132441|gb| extensin {Pisum sativum}, partial (45%)
Length = 381
Score = 38.1 bits (87), Expect = 0.017
Identities = 19/51 (37%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
Frame = +3
Query: 53 NGHLRSIPPQPP-PIYHHNP-APYHNYPSQTQHSALSSPPLKLIKFSGSDP 101
N ++ S PP PP P Y+H+P P H+ P +S+ PP K K++ P
Sbjct: 30 NSYIYSSPPPPPKPYYYHSPPPPVHSPPPPYHYSSPPPPPKKPYKYASPPP 182
>BQ141029 similar to GP|8132441|gb| extensin {Pisum sativum}, partial (54%)
Length = 613
Score = 35.8 bits (81), Expect = 0.084
Identities = 20/64 (31%), Positives = 32/64 (49%), Gaps = 1/64 (1%)
Frame = +2
Query: 39 ISAISGLIDQVSFLNGHLRSIPPQPP-PIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFS 97
++ IS + + N ++ S PP PP P Y+H+P P + P H + S PP + K+
Sbjct: 53 LAIISLTLPSQTSANSYIYSSPPPPPKPYYYHSPPPPVHSPPPPYHYS-SPPPPPVYKYK 229
Query: 98 GSDP 101
P
Sbjct: 230 SPPP 241
>TC92009
Length = 974
Score = 35.0 bits (79), Expect = 0.14
Identities = 23/78 (29%), Positives = 37/78 (46%), Gaps = 3/78 (3%)
Frame = +2
Query: 92 KLIKFSGSDPTFWLLNTEVFFLQHPWPLELRFQFIALYLEGQ-ALTWFNLWRHQLD--SW 148
KL +F+G+DP W+ E+FF + + Q+ + +E + AL WF W + W
Sbjct: 686 KLSQFTGTDPAGWITRAEMFFADNEIHSCDKLQWAFMSMEDEKALLWFYSWCQENPDADW 865
Query: 149 EKFREVFKLQFIQFRSEH 166
+ F +F R EH
Sbjct: 866 KSFSMAMIREF-GARMEH 916
>BE998536 similar to GP|2598593|emb| MtN12 {Medicago truncatula}, partial
(64%)
Length = 448
Score = 35.0 bits (79), Expect = 0.14
Identities = 14/30 (46%), Positives = 14/30 (46%)
Frame = +3
Query: 61 PQPPPIYHHNPAPYHNYPSQTQHSALSSPP 90
P P P YH P P H YP H SPP
Sbjct: 153 PHPKPGYHSPPPPGHTYPPHGPHPGYHSPP 242
>TC86164 weakly similar to GP|20514261|gb|AAM22959.1 tubulin folding cofactor
C {Arabidopsis thaliana}, partial (8%)
Length = 1369
Score = 35.0 bits (79), Expect = 0.14
Identities = 18/47 (38%), Positives = 26/47 (55%)
Frame = -1
Query: 56 LRSIPPQPPPIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDPT 102
++S+ +PP YH +P P PS ++ L+SPPL S S PT
Sbjct: 1315 VKSLESRPPSGYHADPCPSPPAPSLSKSRWLASPPLATRSSSDSVPT 1175
>TC82241 similar to PIR|B84620|B84620 hypothetical protein At2g23070
[imported] - Arabidopsis thaliana, partial (79%)
Length = 1468
Score = 34.3 bits (77), Expect = 0.24
Identities = 26/89 (29%), Positives = 41/89 (45%), Gaps = 4/89 (4%)
Frame = +2
Query: 56 LRSIPPQPPPIYHHNPAPYHNYP----SQTQHSALSSPPLKLIKFSGSDPTFWLLNTEVF 111
++S PP PPP P YH +P SQ + L++P + L + PT WL +E
Sbjct: 62 IQSPPPPPPP----PPTFYHPFPSSVASQLRSHNLAAPNILLRR---RGPTLWLNESENP 220
Query: 112 FLQHPWPLELRFQFIALYLEGQALTWFNL 140
F PL F +++ + + + NL
Sbjct: 221 FAAPALPLRPEFTLMSMLFDLRNIGIMNL 307
>BE325851 similar to PIR|T02573|T025 CDP-diacylglycerol--glycerol-3-phosphate
3-phosphatidyltransferase homolog At2g39290 -
Arabidopsis, partial (29%)
Length = 451
Score = 33.1 bits (74), Expect = 0.54
Identities = 16/59 (27%), Positives = 27/59 (45%)
Frame = +1
Query: 74 YHNYPSQTQHSALSSPPLKLIKFSGSDPTFWLLNTEVFFLQHPWPLELRFQFIALYLEG 132
+HN S+ + PP L+ S P L++ +F LQ + + F F+ +L G
Sbjct: 97 HHNLNSRNLRNCSLCPPFSLLAVSPQSPFLLLVSLSIFHLQLQFHIHFLFMFVYSFLYG 273
>TC76721 homologue to GP|8132441|gb|AAF73291.1| extensin {Pisum sativum},
partial (46%)
Length = 674
Score = 32.7 bits (73), Expect = 0.71
Identities = 21/68 (30%), Positives = 32/68 (46%), Gaps = 5/68 (7%)
Frame = +1
Query: 39 ISAISGLIDQVSFLNGHLRSIPPQPP-PIYHHNPAP---YHNYPSQTQH-SALSSPPLKL 93
++ IS + + N ++ S PP PP P Y+H+P P + YPS SPP +
Sbjct: 40 LAIISLTLPSQTSANSYIYSSPPPPPKPYYYHSPPPPKKPYKYPSPPPPVYKYKSPPPPV 219
Query: 94 IKFSGSDP 101
K+ P
Sbjct: 220 YKYKSPPP 243
>TC93091 similar to GP|4115538|dbj|BAA36412.1 UDP-glycose:flavonoid
glycosyltransferase {Vigna mungo}, partial (37%)
Length = 845
Score = 32.3 bits (72), Expect = 0.92
Identities = 21/52 (40%), Positives = 23/52 (43%), Gaps = 6/52 (11%)
Frame = -3
Query: 55 HLRSIPPQPPPIYHHNPAPYHNYPSQTQHS------ALSSPPLKLIKFSGSD 100
HL PPQ PP Y P P H+Y H+ AL PPL S SD
Sbjct: 441 HLPFAPPQQPPSY---PPPSHSY*HAALHAMAMQSLALQQPPLPTPSSSHSD 295
>TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein T28I19.100
- Arabidopsis thaliana, partial (14%)
Length = 1460
Score = 32.3 bits (72), Expect = 0.92
Identities = 25/77 (32%), Positives = 35/77 (44%)
Frame = -3
Query: 58 SIPPQPPPIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDPTFWLLNTEVFFLQHPW 117
S+ PQP P H+ P H++ +QH L PPL F P LL+ +FL HP
Sbjct: 708 SLHPQPHPHDHYLPLISHDHFHLSQHLLLHLPPL---HFCFHFPPALLLH--AYFLLHPV 544
Query: 118 PLELRFQFIALYLEGQA 134
L + L+ +A
Sbjct: 543 LLSFHAHCLHFLLQFRA 493
>BQ144271 weakly similar to GP|21322711|em pherophorin-dz1 protein {Volvox
carteri f. nagariensis}, partial (5%)
Length = 919
Score = 32.0 bits (71), Expect = 1.2
Identities = 16/39 (41%), Positives = 20/39 (51%), Gaps = 5/39 (12%)
Frame = +1
Query: 56 LRSIPPQPPPIYHH-----NPAPYHNYPSQTQHSALSSP 89
L S PP PPP HH +P P Y S++QH + P
Sbjct: 190 LPSDPPHPPPNTHHPIQAPSPGPPSQYESKSQHINTTHP 306
>AL368332 weakly similar to GP|21322711|e pherophorin-dz1 protein {Volvox
carteri f. nagariensis}, partial (10%)
Length = 478
Score = 32.0 bits (71), Expect = 1.2
Identities = 13/31 (41%), Positives = 19/31 (60%), Gaps = 1/31 (3%)
Frame = +1
Query: 61 PQPPPIYHHNPAPYHNYPSQTQHSALS-SPP 90
P PPP + +P P HN+P + H ++ SPP
Sbjct: 238 PPPPPSHPFSPPPPHNHPPPSPHHPIAPSPP 330
>TC81780 homologue to GP|20521948|dbj|BAB13408. KIAA1582 protein {Homo
sapiens}, partial (1%)
Length = 787
Score = 32.0 bits (71), Expect = 1.2
Identities = 13/32 (40%), Positives = 17/32 (52%)
Frame = +1
Query: 59 IPPQPPPIYHHNPAPYHNYPSQTQHSALSSPP 90
+PP PPP P PY N+ S + + L PP
Sbjct: 55 LPPPPPPQQPPQPPPYQNHSSHSNSNNL*PPP 150
>TC76431 similar to GP|8132441|gb|AAF73291.1| extensin {Pisum sativum},
partial (30%)
Length = 890
Score = 31.6 bits (70), Expect = 1.6
Identities = 17/59 (28%), Positives = 24/59 (39%)
Frame = +1
Query: 60 PPQPPPIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDPTFWLLNTEVFFLQHPWP 118
P PPP +HH P P H SPP K+S P ++ + ++ P P
Sbjct: 136 PSPPPPTHHHYPHP---------HPVYHSPPPPKYKYSSPPPPVHHVSPKPYYHSPPPP 285
Score = 31.2 bits (69), Expect = 2.1
Identities = 14/39 (35%), Positives = 20/39 (50%)
Frame = +1
Query: 63 PPPIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDP 101
PPP++H +P PY++ P PP K K+S P
Sbjct: 229 PPPVHHVSPKPYYHSP---------PPPKKPYKYSSPPP 318
>BQ141680 similar to GP|11993889|gb| virion-associated nuclear-shuttling
protein {Mus musculus}, partial (3%)
Length = 1156
Score = 31.2 bits (69), Expect = 2.1
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -3
Query: 52 LNGHLRSIPPQPPPIYHHNPAP 73
+ G+ RS+PP PPP H P P
Sbjct: 101 VQGYFRSLPPPPPPSVHPTPHP 36
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.137 0.431
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,426,268
Number of Sequences: 36976
Number of extensions: 699459
Number of successful extensions: 5324
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 4667
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5134
length of query: 987
length of database: 9,014,727
effective HSP length: 105
effective length of query: 882
effective length of database: 5,132,247
effective search space: 4526641854
effective search space used: 4526641854
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0181.4