TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0180a.12
         (1023 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC77760 similar to GP|10177333|dbj|BAB10682. 2-oxoglutarate dehy...   424  0.0
TC78694 similar to GP|10177333|dbj|BAB10682. 2-oxoglutarate dehy...   583  e-167
TC77795 similar to GP|15450707|gb|AAK96625.1 At1g01090/T25K16_8 ...    44  4e-04
TC89799 weakly similar to GP|15292931|gb|AAK93576.1 unknown prot...    31  2.1
TC84884 weakly similar to GP|10176728|dbj|BAB09958. gene_id:MXM1...    31  2.8
TC89988 weakly similar to PIR|T49238|T49238 hypothetical protein...    30  3.6
BF005255 similar to GP|18086393|gb At2g31890/F20M17.7 {Arabidops...    30  6.2
TC79475 homologue to PIR|T03406|T03406 probable isocitrate dehyd...    29  8.1

>TC77760 similar to GP|10177333|dbj|BAB10682. 2-oxoglutarate dehydrogenase  E1
            component {Arabidopsis thaliana}, partial (61%)
          Length = 2283

 Score =  424 bits (1091), Expect(5) = 0.0
 Identities = 199/228 (87%), Positives = 214/228 (93%)
 Frame = +2

Query: 462  PIFHVNGDDVESVVHVCELAAEWRQTFHSDVVVDLVCYRRFGHNEIDEPSFTQPKMYKVI 521
            P F + GDDVE+VVH CELAAEWRQTFHSDVVVDLVCYRRFGHNEIDEPSFTQPKMYKVI
Sbjct: 1526 PSFMLTGDDVEAVVHACELAAEWRQTFHSDVVVDLVCYRRFGHNEIDEPSFTQPKMYKVI 1705

Query: 522  RNHPSALEIYQKKLLELGELTQEDIDKIHKKVTSILNDEFLASKDYIPKRRDWLSAYWSG 581
            R+HPS LEIYQKKLLE GELTQ+DIDKIHKKVTSILN+EF ASKDYIPKRRDWLSAYW G
Sbjct: 1706 RSHPSTLEIYQKKLLETGELTQDDIDKIHKKVTSILNEEFQASKDYIPKRRDWLSAYWLG 1885

Query: 582  FKSPEQLSRIRNTGVKPDILKNVGKAITALPESLNPHRAVKKVYEQRAQMVETGEDIDWG 641
            FKSPEQLSR+RNTGVKP+ILK VGKAIT +PE+  PH+AVK++YEQRAQM+ETGEDIDWG
Sbjct: 1886 FKSPEQLSRVRNTGVKPEILKTVGKAITTIPENFTPHKAVKRIYEQRAQMIETGEDIDWG 2065

Query: 642  FAEALAFATLIVEGNHVRLSGQDVERGTFSHRHAVVHDQATGEKYCPL 689
            F EALAFATL+VEGNHVRLSGQDVERGTFSHRH+VVHDQ TGEKYCPL
Sbjct: 2066 FGEALAFATLLVEGNHVRLSGQDVERGTFSHRHSVVHDQTTGEKYCPL 2209



 Score =  282 bits (721), Expect = 5e-76
 Identities = 145/183 (79%), Positives = 160/183 (87%), Gaps = 2/183 (1%)
 Frame = +3

Query: 1   MAWFRAAALSIAKHAIRRNLFRG-GSSYYVTRTTNLPST-SRKLHTTIFKSEAHAAPVPR 58
           MAWFRA A SIAK+AI+R + +   SSY V+R+  LPST  RK HTT+FK +A  APVPR
Sbjct: 138 MAWFRAGA-SIAKNAIKRTISQNRSSSYLVSRSRILPSTHGRKFHTTVFKQQA--APVPR 308

Query: 59  PVPLSRLTDNFLDGTSSAYLEELQRAWEADPSSVDESWDNFFRNFVGQASTSPGISGQTI 118
            VPLSRLTD+FLDGTSS YLEELQRAWEADP+SVDESWDNFFRNFVGQASTSPGISGQTI
Sbjct: 309 AVPLSRLTDSFLDGTSSVYLEELQRAWEADPNSVDESWDNFFRNFVGQASTSPGISGQTI 488

Query: 119 QESMRLLLLVRAYQVNGHMKAKLDPLGLKERNIPDELDPGLYGFTEADLDREFFLGVWNM 178
           QESMRLLL+VRAYQVNGHMKAKLDPL L+ R IPD+LDP LYGF+EADLDREFFLGVW M
Sbjct: 489 QESMRLLLMVRAYQVNGHMKAKLDPLNLEARQIPDDLDPALYGFSEADLDREFFLGVWRM 668

Query: 179 SGF 181
           +GF
Sbjct: 669 AGF 677



 Score =  274 bits (700), Expect(5) = 0.0
 Identities = 132/152 (86%), Positives = 142/152 (92%), Gaps = 1/152 (0%)
 Frame = +1

Query: 317  IFCEFSGGL-PQDEVGLYTGTGDVKYHLGTSYDRPTRGGRRIHLSLMANPSHLEAVNPLV 375
            +F    GGL P+DEVGLYTGTGDVKYHLGTSYDRPTRGG+RIHLSL+ANPSHLEAV+P+V
Sbjct: 1087 LFVSSGGGLSPEDEVGLYTGTGDVKYHLGTSYDRPTRGGKRIHLSLVANPSHLEAVDPVV 1266

Query: 376  VGKTRAKQYYSNDAERMKNMGVLIHGDGSFAGQGVVYETLHLSALPNYTTGGTIHIVFNN 435
            VGKTRAKQYYSND +R KNMG+L+HGDGSFAGQGVVYETLHLSALPNYTTGGTIHIV NN
Sbjct: 1267 VGKTRAKQYYSNDVDRTKNMGILLHGDGSFAGQGVVYETLHLSALPNYTTGGTIHIVLNN 1446

Query: 436  QVAFTTDPESGRSSQYSTDVAKALNAPIFHVN 467
            QVAFTTDP SGRSSQY TDVAKAL+APIFHVN
Sbjct: 1447 QVAFTTDPMSGRSSQYCTDVAKALDAPIFHVN 1542



 Score =  157 bits (396), Expect(5) = 0.0
 Identities = 76/80 (95%), Positives = 79/80 (98%)
 Frame = +2

Query: 239 REVIFDRLAWSTLFENFLATKWTSAKRFGLEGGETLIPGMKEMFDRASDLGVENIVMGMA 298
           RE IFDRLAWS+LFENFLATKWTSAKRFGLEGGETLIPGMKEMFDRASDLGVE+IV+GMA
Sbjct: 851 REAIFDRLAWSSLFENFLATKWTSAKRFGLEGGETLIPGMKEMFDRASDLGVESIVIGMA 1030

Query: 299 HRGRLNVLGNVVRKPLRQIF 318
           HRGRLNVLGNVVRKPLRQIF
Sbjct: 1031HRGRLNVLGNVVRKPLRQIF 1090



 Score =  118 bits (296), Expect(5) = 0.0
 Identities = 57/78 (73%), Positives = 61/78 (78%)
 Frame = +1

Query: 161 GFTEADLDREFFLGVWNMSGFLSENRPVQTLRSILTRLEQAYCGSIGYEYMHIPDRDKCN 220
           G     L   FF       GFLSENRPVQTLRSILTRLEQAYCGSIG+EYMHI DR+KCN
Sbjct: 616 GLVRRILIGSFFWVFGGWPGFLSENRPVQTLRSILTRLEQAYCGSIGFEYMHISDREKCN 795

Query: 221 WLRDKIETPSPTQFSRER 238
           WLRDKIETP+P QF+RER
Sbjct: 796 WLRDKIETPTPVQFNRER 849



 Score = 41.6 bits (96), Expect(5) = 0.0
 Identities = 19/24 (79%), Positives = 22/24 (91%)
 Frame = +1

Query: 688  PLDHVIMNQNEEMFTVSNSSLSEF 711
            PLD+VI+NQ+EEMFT  NSSLSEF
Sbjct: 2203 PLDNVILNQDEEMFTGXNSSLSEF 2274


>TC78694 similar to GP|10177333|dbj|BAB10682. 2-oxoglutarate dehydrogenase  E1
            component {Arabidopsis thaliana}, partial (29%)
          Length = 1224

 Score =  583 bits (1504), Expect = e-167
 Identities = 274/306 (89%), Positives = 296/306 (96%)
 Frame = +3

Query: 718  LGYSMENPNSLVIWEAQFGDFANGAQVIFDNFLSSGESKWLRQTGLVVLLPHGYDGQGPE 777
            LGYSMENPNSL+IWEAQFGDFANGA VIFDNFL+SGE+KWLRQTGLVVLLPHGYDGQGPE
Sbjct: 3    LGYSMENPNSLIIWEAQFGDFANGAHVIFDNFLASGEAKWLRQTGLVVLLPHGYDGQGPE 182

Query: 778  HSSGRLERYLQMADDHPYIIPEMDPTLRKQIQECNLQIVNVTTPANFFHVLRRQIHREFR 837
            HSS RLER+LQMADD+PYIIPEMDPTLRKQIQECNLQIVNVTTPANFFHVLRRQIHREFR
Sbjct: 183  HSSARLERFLQMADDNPYIIPEMDPTLRKQIQECNLQIVNVTTPANFFHVLRRQIHREFR 362

Query: 838  KPLIVMSPKNLLRSKVCRSNLSEFDDVQGHPGFDKQGTRFKRLIKDQNDHSNVEEGIRRL 897
            KPLIVMSPKNLLRSK CRSNLSEFDDVQGHPGFDKQGTRFKRLIKD+NDH+ VEEGIRRL
Sbjct: 363  KPLIVMSPKNLLRSKSCRSNLSEFDDVQGHPGFDKQGTRFKRLIKDRNDHNTVEEGIRRL 542

Query: 898  ILCSGKVYYELDEQRTKDDAKDVAICRVEQLCPFPYDLVQRELKRYPNAEVVWCQEEPMN 957
            +LCSGKVYYELD+ R+K DA DVAICRVEQLCPFPYDLVQRELKRYPNAEVVWCQEEPMN
Sbjct: 543  VLCSGKVYYELDDHRSKVDASDVAICRVEQLCPFPYDLVQRELKRYPNAEVVWCQEEPMN 722

Query: 958  MGGYSYILPRLITSMKALGRGGYEDVKYVGRAPSAATATGFLKVHQREQAELVHKAMQHK 1017
            MGGY+YILPRL++SMKA+GRGG++DVKYVGRAPSAATATGFLKVHQ+EQAE+  KA+Q +
Sbjct: 723  MGGYTYILPRLVSSMKAVGRGGFDDVKYVGRAPSAATATGFLKVHQKEQAEIAEKALQRE 902

Query: 1018 PINFPY 1023
            P+NFP+
Sbjct: 903  PVNFPF 920


>TC77795 similar to GP|15450707|gb|AAK96625.1 At1g01090/T25K16_8 {Arabidopsis
            thaliana}, partial (82%)
          Length = 1690

 Score = 43.5 bits (101), Expect = 4e-04
 Identities = 51/192 (26%), Positives = 80/192 (41%), Gaps = 10/192 (5%)
 Frame = +2

Query: 394  NMGVLIHGDGSFAGQGVVYETLHLSALPNYTTGGTIHIVFNNQVAF-------TTDPESG 446
            N+ +   GDG+    G  +E L+++AL        + +V NN  A        T+DPE  
Sbjct: 830  NVTLAFFGDGT-CNNGQFFECLNMAALWKLPI---VFVVENNLWAIGMSHLRATSDPEIW 997

Query: 447  RSSQYSTDVAKALNAPIFHVNGDDVESVVHVCELAAEWRQTFHSDVVVDLVCYRRFGHNE 506
            +          A   P  HV+G DV  V  V + A +  +      +V+   YR  GH+ 
Sbjct: 998  KKGP-------AFGMPGVHVDGMDVLKVREVAKEAIDRARRGEGPSLVECETYRFRGHSL 1156

Query: 507  IDEPSFTQP--KMYKVIRNHPSALEIYQKKLLELGELTQEDIDKIHKKVTSILNDEF-LA 563
             D      P  K +   R+  +AL+ Y   L E    T++++  I KK+  +L +    A
Sbjct: 1157 ADPDELRNPAEKQHYAGRDPITALKKY---LFENKLATEQELKAIDKKIDEVLEEAVDFA 1327

Query: 564  SKDYIPKRRDWL 575
             K   P R   L
Sbjct: 1328 EKSPAPARSQLL 1363


>TC89799 weakly similar to GP|15292931|gb|AAK93576.1 unknown protein
           {Arabidopsis thaliana}, partial (92%)
          Length = 622

 Score = 31.2 bits (69), Expect = 2.1
 Identities = 13/30 (43%), Positives = 18/30 (59%)
 Frame = +2

Query: 640 WGFAEALAFATLIVEGNHVRLSGQDVERGT 669
           WG  E ++ AT   E NH  + GQ++ RGT
Sbjct: 437 WGVCEQISQATCDKERNHATIFGQELRRGT 526


>TC84884 weakly similar to GP|10176728|dbj|BAB09958.
           gene_id:MXM12.18~unknown protein {Arabidopsis thaliana},
           partial (5%)
          Length = 688

 Score = 30.8 bits (68), Expect = 2.8
 Identities = 24/76 (31%), Positives = 34/76 (44%), Gaps = 4/76 (5%)
 Frame = +2

Query: 671 SHRHAVVHD----QATGEKYCPLDHVIMNQNEEMFTVSNSSLSEFGVLGFELGYSMENPN 726
           SH  A  HD     AT ++      VI +  E    VS+S+ +    +    G+S+ NP 
Sbjct: 56  SHLSAKPHDGHDTNATSQE------VIGDDQENYLNVSDSNKA----ISTRSGHSLINPQ 205

Query: 727 SLVIWEAQFGDFANGA 742
               W  Q+G F NGA
Sbjct: 206 MAPSWFEQYGTFKNGA 253


>TC89988 weakly similar to PIR|T49238|T49238 hypothetical protein F7K15.90 -
           Arabidopsis thaliana, partial (12%)
          Length = 799

 Score = 30.4 bits (67), Expect = 3.6
 Identities = 36/129 (27%), Positives = 55/129 (41%), Gaps = 2/129 (1%)
 Frame = +1

Query: 667 RGTFSHRHAVVHDQATGEKYCPLDHVIMNQNEEMFTVSNSSLSEF--GVLGFELGYSMEN 724
           +G  S  H  + ++       PL HV++N++    + S  SLS F  GV  F+L +++  
Sbjct: 175 KGCCSFSHKELLNKLVLFSLLPLKHVLLNRSSYRISRSILSLSWFLRGVSRFKL-FAIRK 351

Query: 725 PNSLVIWEAQFGDFANGAQVIFDNFLSSGESKWLRQTGLVVLLPHGYDGQGPEHSSGRLE 784
            NS V        F N    I   F   G S W+R+      L H +   G   +S  L+
Sbjct: 352 RNSFV-------KFLNRVNQIL--FTFKGSSYWMRK------LVHWF---GKVENSPTLK 477

Query: 785 RYLQMADDH 793
           RY   +  H
Sbjct: 478 RYQSSSIPH 504


>BF005255 similar to GP|18086393|gb At2g31890/F20M17.7 {Arabidopsis
           thaliana}, partial (28%)
          Length = 702

 Score = 29.6 bits (65), Expect = 6.2
 Identities = 18/69 (26%), Positives = 30/69 (43%), Gaps = 2/69 (2%)
 Frame = +2

Query: 167 LDREFFLGVWNMSGFLSENRPVQTLRSILTRLEQAYCGS--IGYEYMHIPDRDKCNWLRD 224
           +DR FF  VW   G+  E R  +  R  +    Q +  +  +  E+ H+     C  L D
Sbjct: 209 MDRSFFSYVWKTLGYFEEQRVSELYREDIMFASQVHLVNQCLKLEFPHL-QLSLCGELED 385

Query: 225 KIETPSPTQ 233
           K+ +   T+
Sbjct: 386 KVSSAGKTK 412


>TC79475 homologue to PIR|T03406|T03406 probable isocitrate dehydrogenase
            (NAD+) (EC 1.1.1.41) precursor - common tobacco, partial
            (90%)
          Length = 1495

 Score = 29.3 bits (64), Expect = 8.1
 Identities = 22/75 (29%), Positives = 35/75 (46%), Gaps = 3/75 (4%)
 Frame = +2

Query: 942  RYPNAEVVWCQEEPMNMGGYSYILPRLITSMKALGRGGYEDVKYVGRAPS---AATATGF 998
            RY N +++  +E     G YS +  +++       RG  E +K + R  S   A  A  +
Sbjct: 482  RYDNVDLITIREN--TEGEYSGLEHQVV-------RGVVESIKIITRQASLRVAEYAFHY 634

Query: 999  LKVHQREQAELVHKA 1013
             K H RE+   +HKA
Sbjct: 635  AKAHGRERVSAIHKA 679


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.319    0.137    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,652,390
Number of Sequences: 36976
Number of extensions: 423188
Number of successful extensions: 1841
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1818
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1839
length of query: 1023
length of database: 9,014,727
effective HSP length: 106
effective length of query: 917
effective length of database: 5,095,271
effective search space: 4672363507
effective search space used: 4672363507
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)