BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0177.9
(113 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI310662 homologue to SP|P12227|RPO DNA-directed RNA polymerase ... 202 2e-53
TC78992 similar to GP|20466530|gb|AAM20582.1 protein kinase-like... 26 2.6
TC86941 similar to GP|15294290|gb|AAK95322.1 AT5g02240/T7H20_290... 25 4.5
TC81427 weakly similar to GP|11994216|dbj|BAB01338. disease resi... 25 5.8
TC92273 similar to GP|21622503|emb|CAD37050. related to OSBP-rel... 25 5.8
TC80383 similar to PIR|C85065|C85065 kinesin-like protein [impor... 25 5.8
TC77539 similar to GP|16118856|gb|AAL14629.1 growth-on protein G... 24 7.6
TC81990 24 10.0
TC92963 homologue to SP|O04865|PLD_VIGUN Phospholipase D precurs... 24 10.0
BG588641 similar to GP|20197696|gb| senescence-associated protei... 24 10.0
>BI310662 homologue to SP|P12227|RPO DNA-directed RNA polymerase beta' chain
(EC 2.7.7.6) (Fragment). [Garden pea] {Pisum sativum},
partial (19%)
Length = 688
Score = 202 bits (514), Expect = 2e-53
Identities = 105/113 (92%), Positives = 110/113 (96%)
Frame = +2
Query: 1 AQSRISLVNKIQRVYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLGELIGLLRA 60
AQSRISLVNKIQ+VYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVF GELIGLLRA
Sbjct: 344 AQSRISLVNKIQKVYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFLPGELIGLLRA 523
Query: 61 QRTGRALEESICYRTILLGITKTSMNTQSFISEASFQETARVLAKAALRGRID 113
+RTGRALEE+ICYR +LLGITKTS+NTQSFISEASFQETARVLAKAALRGRID
Sbjct: 524 ERTGRALEEAICYRALLLGITKTSLNTQSFISEASFQETARVLAKAALRGRID 682
>TC78992 similar to GP|20466530|gb|AAM20582.1 protein kinase-like protein
{Arabidopsis thaliana}, partial (73%)
Length = 1801
Score = 25.8 bits (55), Expect = 2.6
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = -1
Query: 6 SLVNKIQRVYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLG 52
S VNK ++ +++ IHN+HI V S + + N+ LG
Sbjct: 394 STVNK--KILKTRAQQIHNKHIVFPVYTTPSYAWDTTSSLKNIIELG 260
>TC86941 similar to GP|15294290|gb|AAK95322.1 AT5g02240/T7H20_290
{Arabidopsis thaliana}, partial (98%)
Length = 1121
Score = 25.0 bits (53), Expect = 4.5
Identities = 14/51 (27%), Positives = 25/51 (48%)
Frame = -2
Query: 5 ISLVNKIQRVYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLGELI 55
IS+ +QR+ R H N+H + R ++S L+ N+F + +I
Sbjct: 556 ISISQTVQRMVRVCSTHRPNQHNMLHSRSLSSIYLIFLSNPVNLFGICTII 404
>TC81427 weakly similar to GP|11994216|dbj|BAB01338. disease resistance
comples protein {Arabidopsis thaliana}, partial (6%)
Length = 1253
Score = 24.6 bits (52), Expect = 5.8
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3
Query: 33 QITSKVLVSEDGMSNVFSLGELIGL 57
Q + +VS DG SNV LGE++ L
Sbjct: 378 QSLPRFVVSNDGGSNVGELGEMLEL 452
>TC92273 similar to GP|21622503|emb|CAD37050. related to OSBP-related
protein 7 {Neurospora crassa}, partial (36%)
Length = 1517
Score = 24.6 bits (52), Expect = 5.8
Identities = 20/66 (30%), Positives = 28/66 (42%), Gaps = 4/66 (6%)
Frame = +3
Query: 51 LGELIGLLRAQRTG----RALEESICYRTILLGITKTSMNTQSFISEASFQETARVLAKA 106
LGE LLR + R L E +C+R + L + S N S A Q+ A+
Sbjct: 480 LGETFELLRTEGEVPGGFRLLVEKVCHRPVRLAMQADSSNWSFSQSPAPSQKFWGKSAEI 659
Query: 107 ALRGRI 112
GR+
Sbjct: 660 TTDGRV 677
>TC80383 similar to PIR|C85065|C85065 kinesin-like protein [imported] -
Arabidopsis thaliana, partial (26%)
Length = 897
Score = 24.6 bits (52), Expect = 5.8
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +3
Query: 67 LEESICYRTILLGITKTSMNTQSFIS 92
LEESIC + ITK S N++ S
Sbjct: 675 LEESICLAYVTTTITKISFNSKFLTS 752
>TC77539 similar to GP|16118856|gb|AAL14629.1 growth-on protein GRO10
{Euphorbia esula}, partial (44%)
Length = 2020
Score = 24.3 bits (51), Expect = 7.6
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +2
Query: 58 LRAQRTGRALEESICYRTILLGITK 82
LR + + LE IC+ T+ +G+TK
Sbjct: 1355 LRT*NSKKRLEGLICWNTLSIGVTK 1429
>TC81990
Length = 627
Score = 23.9 bits (50), Expect = 10.0
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -1
Query: 10 KIQRVYRSQGVHIHNRHIEIIVRQI 34
K RVY + VH+H I + VR I
Sbjct: 621 KKTRVYLYEHVHLHVSEISLFVRSI 547
>TC92963 homologue to SP|O04865|PLD_VIGUN Phospholipase D precursor (EC
3.1.4.4) (PLD) (Choline phosphatase), partial (28%)
Length = 741
Score = 23.9 bits (50), Expect = 10.0
Identities = 16/56 (28%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
Frame = +3
Query: 28 EIIVRQITSKVLVSEDGMSNVFSLGELIGLL-RAQRTGRALEESICYRTILLGITK 82
+I+++ + L E +S++ + LI +L QRT L+ SI +LLG+ +
Sbjct: 408 QILLKMLQEPGLSVERIISSIVAFKMLILMLFGVQRTSSILKISISLEALLLGVVR 575
>BG588641 similar to GP|20197696|gb| senescence-associated protein
{Arabidopsis thaliana}, partial (41%)
Length = 516
Score = 23.9 bits (50), Expect = 10.0
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = -3
Query: 22 IHNRHIEIIVRQITSKVLVSEDGMSNVFSL 51
+H+ HI+IIV + +S+ LV+ N++ L
Sbjct: 100 LHSPHIKIIVTR*SSRSLVAGHLRYNIYDL 11
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.134 0.352
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,431,024
Number of Sequences: 36976
Number of extensions: 21851
Number of successful extensions: 123
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of query: 113
length of database: 9,014,727
effective HSP length: 89
effective length of query: 24
effective length of database: 5,723,863
effective search space: 137372712
effective search space used: 137372712
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0177.9