BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0173b.2
(347 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82864 similar to GP|3695061|gb|AAC62625.1| rac GTPase activati... 33 0.12
TC92575 weakly similar to GP|21428434|gb|AAM49877.1 LD13350p {Dr... 32 0.28
TC82080 similar to GP|9757854|dbj|BAB08488.1 contains similarity... 32 0.28
AW683922 weakly similar to GP|10178134|db gene_id:MOP10.11~pir||... 32 0.36
AW690594 similar to GP|23498163|emb hypothetical protein {Plasmo... 32 0.36
TC92456 weakly similar to PIR|T46707|T46707 proteophosphoglycan ... 32 0.47
BF641220 weakly similar to PIR|G86203|G86 probable N-arginine di... 32 0.47
TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown prot... 32 0.47
TC88780 weakly similar to PIR|T05688|T05688 hypothetical protein... 32 0.47
TC89779 GP|12835923|dbj|BAB23419. data source:MGD source key:MG... 31 0.62
TC86645 similar to PIR|H86321|H86321 hypothetical protein AAF271... 31 0.62
TC87242 homologue to PIR|S56716|S56716 protein kinase SPK-3 (EC ... 31 0.81
TC79886 similar to GP|12963432|gb|AAK11263.1 ribosomal protein P... 30 1.1
AW683905 weakly similar to GP|9757854|dbj| contains similarity t... 30 1.1
TC81011 similar to GP|8489806|gb|AAF75761.1| chloroplast protein... 30 1.4
TC80464 weakly similar to GP|13548328|emb|CAC35875. putative pro... 30 1.4
TC77681 similar to GP|22597156|gb|AAN03465.1 nucleolar histone d... 30 1.4
CA920710 similar to PIR|S64314|S643 probable membrane protein YG... 30 1.8
TC77828 similar to GP|16648867|gb|AAL24285.1 HSP associated prot... 30 1.8
BG646141 weakly similar to GP|18568256|gb| putative protein {Sor... 29 2.3
>TC82864 similar to GP|3695061|gb|AAC62625.1| rac GTPase activating protein
2 {Lotus japonicus}, partial (14%)
Length = 583
Score = 33.5 bits (75), Expect = 0.12
Identities = 19/56 (33%), Positives = 29/56 (50%)
Frame = +2
Query: 144 YGVVEDMPSTPVKVYNSEEEEVEADDEVQEDEAQEDDNKDLGSATTIDPDEIDEIY 199
+ V E P+ V +EE+ E ++E +E+E + DDN D D+ DEIY
Sbjct: 224 FSVSEFNPAAQVTNNKKKEEQQEQEEEDEEEELESDDN---------DNDDDDEIY 364
>TC92575 weakly similar to GP|21428434|gb|AAM49877.1 LD13350p {Drosophila
melanogaster}, partial (3%)
Length = 557
Score = 32.3 bits (72), Expect = 0.28
Identities = 35/160 (21%), Positives = 65/160 (39%), Gaps = 19/160 (11%)
Frame = -1
Query: 161 EEEEVEADDEVQEDEAQ----EDD--------NKDLGSATTIDPDEIDEIYRDGGLEGLR 208
E+++V+A DE++ED+ EDD +K+ + E+D + + LEG
Sbjct: 521 EDKKVDAGDEIEEDKKDDNGFEDDKLSEETELSKEERESVEAKKPELDAMDEESILEGKD 342
Query: 209 SGGDARIVAVQGGDGISPEEVAVKNNQPE-------KKESFGPNSNSNSLSLMAALNSVA 261
G + D ++V K+N+ KK G + N M L +
Sbjct: 341 EGSEKEKSQKDRED--DKDKVDNKSNEENIKVEKRLKKRGKGKVNGENVKKKMKELKKIE 168
Query: 262 EQKEQLRDIIDAGYSLEEIEGWMENLKADGILSKDKQIMD 301
+ E + I+ S +E G E + + + K K++ +
Sbjct: 167 DIPEAKDESIEKERSRDEEVGDKEKVGGENVKKKMKELKE 48
>TC82080 similar to GP|9757854|dbj|BAB08488.1 contains similarity to
apoptosis antagonizing transcription
factor~gene_id:MFB13.10, partial (37%)
Length = 908
Score = 32.3 bits (72), Expect = 0.28
Identities = 37/168 (22%), Positives = 69/168 (41%)
Frame = +2
Query: 136 EKDVEAHDYGVVEDMPSTPVKVYNSEEEEVEADDEVQEDEAQEDDNKDLGSATTIDPDEI 195
+ D++ +D + + + +V EEE+ E DE +EDE QE+ ++ + +
Sbjct: 62 DSDLDEYDDDMSFNEVNDDSEVEEEEEEDDEGSDEEEEDERQEESRWKDDEMEQLEKEYM 241
Query: 196 DEIYRDGGLEGLRSGGDARIVAVQGGDGISPEEVAVKNNQPEKKESFGPNSNSNSLSLMA 255
D +++ L L++ + + G + ++ + P SNSN L +
Sbjct: 242 DLHHQEQELNTLKNLKHHKDEDLLKGQAVKTQKALWYKILELRFLLQKPFSNSNRLPQES 421
Query: 256 ALNSVAEQKEQLRDIIDAGYSLEEIEGWMENLKADGILSKDKQIMDKN 303
S E E +R G + I E L D IL + ++ KN
Sbjct: 422 IKESFCESDETVR-----GAYEDLITSSKETL--DSILELQEALIAKN 544
>AW683922 weakly similar to GP|10178134|db
gene_id:MOP10.11~pir||T04661~similar to unknown protein
{Arabidopsis thaliana}, partial (5%)
Length = 678
Score = 32.0 bits (71), Expect = 0.36
Identities = 41/166 (24%), Positives = 71/166 (42%), Gaps = 5/166 (3%)
Frame = +3
Query: 152 STPVKVYNSEEEEVEAD-DEVQEDEAQEDDNKDLGSATTIDPD----EIDEIYRDGGLEG 206
S P V S E+EVE D D++Q DE Q +A+T PD + D++ D E
Sbjct: 210 SLPTVVVVSNEKEVELDIDDIQIDEPQT-------TASTSSPDVKNKQKDKLQSDK--EK 362
Query: 207 LRSGGDARIVAVQGGDGISPEEVAVKNNQPEKKESFGPNSNSNSLSLMAALNSVAEQKEQ 266
L GG V + +PEE+ + S + + + L ++++
Sbjct: 363 LFQGGTNDDVKPRVR---TPEEIMAAYRKTGDAASVASQTRNKLMERQEKLERISQRTAD 533
Query: 267 LRDIIDAGYSLEEIEGWMENLKADGILSKDKQIMDKNIQNQEKRYS 312
L++ E + +L G K++ + + +IQN EK+Y+
Sbjct: 534 LQN------GAEILHH*QMSLSDHG---KEEMVANIDIQNSEKKYT 644
>AW690594 similar to GP|23498163|emb hypothetical protein {Plasmodium
falciparum 3D7}, partial (10%)
Length = 633
Score = 32.0 bits (71), Expect = 0.36
Identities = 18/64 (28%), Positives = 33/64 (51%)
Frame = +3
Query: 135 VEKDVEAHDYGVVEDMPSTPVKVYNSEEEEVEADDEVQEDEAQEDDNKDLGSATTIDPDE 194
VE++ + HD ++ + EEE+ D+E +E+E +E++ +D + DE
Sbjct: 78 VEEEEDEHDEEEEDEHDEEEEEEEEEEEEDEHDDEEEEEEEEEEEEEED--DDEEGEEDE 251
Query: 195 IDEI 198
ID I
Sbjct: 252 IDRI 263
Score = 29.6 bits (65), Expect = 1.8
Identities = 15/60 (25%), Positives = 30/60 (50%)
Frame = +3
Query: 136 EKDVEAHDYGVVEDMPSTPVKVYNSEEEEVEADDEVQEDEAQEDDNKDLGSATTIDPDEI 195
E++ + HD E+ ++ EEEE E ++E +E++ E+ +D + PD +
Sbjct: 105 EEEEDEHDEEEEEEEEEEEEDEHDDEEEEEEEEEEEEEEDDDEEGEEDEIDRISTLPDPL 284
>TC92456 weakly similar to PIR|T46707|T46707 proteophosphoglycan
membrane-associated [imported] - Leishmania major
(fragment), partial (10%)
Length = 538
Score = 31.6 bits (70), Expect = 0.47
Identities = 23/80 (28%), Positives = 35/80 (43%)
Frame = -1
Query: 118 HCFTMAVDVGGTSFIILVEKDVEAHDYGVVEDMPSTPVKVYNSEEEEVEADDEVQEDEAQ 177
H F + D+ +L K E + + + + PV E+E+V DE ED AQ
Sbjct: 316 HLFKLLNDI-----TVLFSKTAEQFNAFKSQHLINNPVVPQEQEQEQVV--DEDGEDAAQ 158
Query: 178 EDDNKDLGSATTIDPDEIDE 197
EDDN D +E ++
Sbjct: 157 EDDNAAAEEGDAADEEEEED 98
>BF641220 weakly similar to PIR|G86203|G86 probable N-arginine dibasic
convertase [imported] - Arabidopsis thaliana, partial
(5%)
Length = 634
Score = 31.6 bits (70), Expect = 0.47
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = +2
Query: 161 EEEEVEADDEVQEDEAQEDDNKDLGSATTIDPDEIDEIYRDGG 203
E++E E D++ ++DE +D+ +D D DE R+GG
Sbjct: 362 EDDEEEDDEDEEDDEEDDDEGEDDEDEEXEDEDEXXVXGREGG 490
>TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown protein
{Arabidopsis thaliana}, partial (19%)
Length = 679
Score = 31.6 bits (70), Expect = 0.47
Identities = 22/93 (23%), Positives = 40/93 (42%), Gaps = 12/93 (12%)
Frame = +1
Query: 159 NSEEEEVEADDEVQEDEAQEDDNKDLGSATTID-----------PDEIDEIYRDGGLEGL 207
+ +E+E E DD+ +D+ ++DD+ T ++ PD E ++ G+ L
Sbjct: 265 DEDEDEDEDDDDDDDDDDEDDDDDGFAEPTDLNAKDKRLKSKTVPDRQQEKEKEKGVRSL 444
Query: 208 RSGGDARIVAVQGGDGISPEEVA-VKNNQPEKK 239
+G R+ Q + A + N EKK
Sbjct: 445 NNGQSKRLPKSQRIASLQENNSAKFRRNSMEKK 543
>TC88780 weakly similar to PIR|T05688|T05688 hypothetical protein F20M13.160
- Arabidopsis thaliana, partial (22%)
Length = 800
Score = 31.6 bits (70), Expect = 0.47
Identities = 26/112 (23%), Positives = 50/112 (44%), Gaps = 3/112 (2%)
Frame = +1
Query: 148 EDMPSTPVKVYNSEEEEVEADDEVQEDEAQEDDNKDL---GSATTIDPDEIDEIYRDGGL 204
ED+ +PVK+ E +E DD++ +DE +DD+ D+ S P+++ ++
Sbjct: 1 EDLDISPVKI--DEALVIEDDDDISDDE--DDDHDDMLRDDSLPVCLPEKVHDVKLGDSA 168
Query: 205 EGLRSGGDARIVAVQGGDGISPEEVAVKNNQPEKKESFGPNSNSNSLSLMAA 256
E + G S + +V+ + P S +S+ ++S AA
Sbjct: 169 EESTATPATNDGQTNAASGSSSKVGSVRGSDPTDFRSGYSSSSRGAMSFAAA 324
>TC89779 GP|12835923|dbj|BAB23419. data source:MGD source key:MGI:1333811
evidence:ISS~methyl-CpG binding domain protein
1~putative, partial (2%)
Length = 824
Score = 31.2 bits (69), Expect = 0.62
Identities = 25/84 (29%), Positives = 43/84 (50%), Gaps = 15/84 (17%)
Frame = +1
Query: 130 SFIILVEKDVEAHDYG--VVEDMPSTPVKVYNSEEEEVEADDEVQ----EDEAQ---EDD 180
+F + E D + DY + ED S P Y+SEE++V+ E + E+E++ E D
Sbjct: 364 NFHHIEESDDDKRDYNERISEDRESVPQ--YSSEEKKVDESSERESVHGEEESESEGEQD 537
Query: 181 NKDLGSA------TTIDPDEIDEI 198
N D+G + + I+P D++
Sbjct: 538 NSDVGDSPGETERSHIEPSSPDDV 609
>TC86645 similar to PIR|H86321|H86321 hypothetical protein AAF27100.1
[imported] - Arabidopsis thaliana, partial (76%)
Length = 1116
Score = 31.2 bits (69), Expect = 0.62
Identities = 15/53 (28%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
Frame = +3
Query: 146 VVEDMPSTPVKVYNSEE-EEVEADDEVQEDEAQEDDNKDLGSATTIDPDEIDE 197
+ +D+ P+ +NSE+ +E E DD+ D ++DD+ + D D+ +E
Sbjct: 678 IKDDLWPNPLNYFNSEDPDEAEEDDDEAGDAGKDDDDSEDDDDQEDDDDDEEE 836
>TC87242 homologue to PIR|S56716|S56716 protein kinase SPK-3 (EC 2.7.1.-) -
soybean, complete
Length = 1659
Score = 30.8 bits (68), Expect = 0.81
Identities = 15/32 (46%), Positives = 22/32 (67%), Gaps = 3/32 (9%)
Frame = +1
Query: 152 STPVKVYN---SEEEEVEADDEVQEDEAQEDD 180
S PVK + EEEE E ++EV+E+E +ED+
Sbjct: 1384 SRPVKGFGWEGEEEEEEEVEEEVEEEEDEEDE 1479
>TC79886 similar to GP|12963432|gb|AAK11263.1 ribosomal protein P2
{Podospora anserina}, partial (82%)
Length = 592
Score = 30.4 bits (67), Expect = 1.1
Identities = 23/77 (29%), Positives = 34/77 (43%)
Frame = +2
Query: 165 VEADDEVQEDEAQEDDNKDLGSATTIDPDEIDEIYRDGGLEGLRSGGDARIVAVQGGDGI 224
+EAD D + D KD+ TI ++ + GG G+ SGG A G
Sbjct: 137 IEADSGRISDLLAQLDGKDINELATIGSTKLATVPAGGG--GVSSGGGG---AAASGGAP 301
Query: 225 SPEEVAVKNNQPEKKES 241
+ EE + + EK+ES
Sbjct: 302 AQEEKKEEAKEEEKEES 352
>AW683905 weakly similar to GP|9757854|dbj| contains similarity to apoptosis
antagonizing transcription factor~gene_id:MFB13.10,
partial (12%)
Length = 492
Score = 30.4 bits (67), Expect = 1.1
Identities = 18/66 (27%), Positives = 33/66 (49%)
Frame = +3
Query: 136 EKDVEAHDYGVVEDMPSTPVKVYNSEEEEVEADDEVQEDEAQEDDNKDLGSATTIDPDEI 195
+ D + +Y +DM V + EEE E D E DE +EDD ++ + +++
Sbjct: 117 KSDSDLDEYDDDDDMSYDEVNDDSELEEEKEEDGE---DEGEEDDGQEENGWRDDEMEQL 287
Query: 196 DEIYRD 201
++ Y+D
Sbjct: 288 EKEYKD 305
>TC81011 similar to GP|8489806|gb|AAF75761.1| chloroplast protein import
component Toc159 {Pisum sativum}, partial (17%)
Length = 808
Score = 30.0 bits (66), Expect = 1.4
Identities = 31/119 (26%), Positives = 56/119 (47%), Gaps = 4/119 (3%)
Frame = +3
Query: 156 KVYNSEEEEVEADDEVQEDEAQEDDNKDLGSATTIDPDEIDEIYRDGGLEGLRSGGDARI 215
++ +EEVE+DDE D + D L + E DGG + + +R+
Sbjct: 207 QIVTDSDEEVESDDE--GDSKELFDTATLAALLKAASGAGGE---DGGGITITAQDGSRL 371
Query: 216 VAVQGGDGISPE----EVAVKNNQPEKKESFGPNSNSNSLSLMAALNSVAEQKEQLRDI 270
+V+ G+ P + AV++N+P FGP S S + ++++ N E+K +L +
Sbjct: 372 FSVERPAGLGPSLQTGKPAVRSNRP---NLFGP-SMSRAGTVVSDTNLSVEEKMKLEKL 536
>TC80464 weakly similar to GP|13548328|emb|CAC35875. putative protein
{Arabidopsis thaliana}, partial (19%)
Length = 1437
Score = 30.0 bits (66), Expect = 1.4
Identities = 16/48 (33%), Positives = 24/48 (49%)
Frame = +3
Query: 142 HDYGVVEDMPSTPVKVYNSEEEEVEADDEVQEDEAQEDDNKDLGSATT 189
HD GV+ DM KV + +DD+ + + +DD+KD S T
Sbjct: 669 HD-GVISDMDYFKSKVTTEWSDSESSDDDSSDSASSDDDDKDKHSHAT 809
>TC77681 similar to GP|22597156|gb|AAN03465.1 nucleolar histone deacetylase
HD2-P39 {Glycine max}, partial (47%)
Length = 1233
Score = 30.0 bits (66), Expect = 1.4
Identities = 13/43 (30%), Positives = 24/43 (55%)
Frame = +2
Query: 155 VKVYNSEEEEVEADDEVQEDEAQEDDNKDLGSATTIDPDEIDE 197
VKV + ++E+ + DDE ++ DD + + + D D+ DE
Sbjct: 548 VKVVDPKDEDSDDDDESDDEIGSSDDEMENADSDSEDEDDSDE 676
>CA920710 similar to PIR|S64314|S643 probable membrane protein YGR023w -
yeast (Saccharomyces cerevisiae), partial (7%)
Length = 774
Score = 29.6 bits (65), Expect = 1.8
Identities = 23/59 (38%), Positives = 32/59 (53%), Gaps = 1/59 (1%)
Frame = -2
Query: 141 AHDYGVVEDMPSTPVKV-YNSEEEEVEADDEVQEDEAQEDDNKDLGSATTIDPDEIDEI 198
A DY VE + TP K Y E E E ++E ++DE ED+N+D D DE +E+
Sbjct: 437 AMDY--VESLVPTPWKSGYIEAEAEAEEEEEEEKDE-DEDENEDEDE----DEDEDEEV 282
>TC77828 similar to GP|16648867|gb|AAL24285.1 HSP associated protein like
{Arabidopsis thaliana}, partial (83%)
Length = 1587
Score = 29.6 bits (65), Expect = 1.8
Identities = 22/81 (27%), Positives = 39/81 (47%), Gaps = 7/81 (8%)
Frame = +3
Query: 137 KDVEAHDYGVVEDMPSTPVKVYNSEEEE--VEADDEVQEDEAQEDDNK-----DLGSATT 189
+ VE+ D +ED+ VKV EEE+ +E+D E++ D + DD+ D T
Sbjct: 258 RSVESDDD--IEDVEEEKVKVEEVEEEDEIIESDVELEGDTVEPDDDPPQKMGDSSVEVT 431
Query: 190 IDPDEIDEIYRDGGLEGLRSG 210
+ E ++ + +E + G
Sbjct: 432 EESRESSQLAKAKAMEAISEG 494
>BG646141 weakly similar to GP|18568256|gb| putative protein {Sorghum
bicolor}, partial (8%)
Length = 775
Score = 29.3 bits (64), Expect = 2.3
Identities = 19/62 (30%), Positives = 30/62 (47%)
Frame = +2
Query: 128 GTSFIILVEKDVEAHDYGVVEDMPSTPVKVYNSEEEEVEADDEVQEDEAQEDDNKDLGSA 187
G F+ L V +H+ + V +N++EEE+ DDE +EDE+ + L S
Sbjct: 422 GRLFLFLQVVSVPSHNNFFTKRQNGRLVLSFNNDEEEI--DDEFEEDESVIEKTHMLSSE 595
Query: 188 TT 189
T
Sbjct: 596 IT 601
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.135 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,616,889
Number of Sequences: 36976
Number of extensions: 138385
Number of successful extensions: 1204
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 999
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1119
length of query: 347
length of database: 9,014,727
effective HSP length: 97
effective length of query: 250
effective length of database: 5,428,055
effective search space: 1357013750
effective search space used: 1357013750
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0173b.2