BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0173a.1
(217 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 74 4e-14
BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [impo... 66 1e-11
BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - ... 64 6e-11
TC90516 55 2e-08
BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At... 55 2e-08
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 52 1e-07
AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase... 50 5e-07
TC79660 similar to PIR|T05057|T05057 hypothetical protein M3E9.1... 29 1.6
TC90966 similar to GP|9758285|dbj|BAB08809.1 gene_id:MLE2.7~unkn... 28 2.7
AL380167 homologue to PIR|S55887|S558 CCHH finger protein 7 - Ar... 28 3.5
TC81941 similar to GP|15420162|gb|AAK97303.1 F-box containing pr... 27 6.0
TC77390 similar to GP|2462925|emb|CAA03884.1 GTP cyclohydrolase ... 27 6.0
TC78188 similar to PIR|C84870|C84870 probable splicing factor [i... 27 7.9
BG648169 similar to GP|8953386|emb| putative protein {Arabidopsi... 27 7.9
TC89782 similar to GP|21618145|gb|AAM67195.1 unknown {Arabidopsi... 27 7.9
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 73.9 bits (180), Expect = 4e-14
Identities = 38/116 (32%), Positives = 70/116 (59%)
Frame = +3
Query: 102 FTSEEIWEVVIACDGNRALGPYGFNFIFFREFWDIIKDDMMKLFVEFYEFGKLVEGLNLK 161
FT+ E+ + + D ++A G G+N FF+ W+II D ++ ++F++ G + + +N
Sbjct: 24 FTAVEVKNALFSMDSSKAPGIDGYNVHFFKCSWNIIGDSVIDAILDFFKTGFMPKIINCT 203
Query: 162 FIALIPKKKILEAVSVYRPISLTGSIYILIAKDLT*RFHMVMPYLLSWNQFSFTKG 217
++ L+PK+ + +V +RPI+ IY +I+K LT R V+ ++S NQ +F KG
Sbjct: 204 YVTLLPKEVNVTSVKNFRPIACCSVIYKIISKILTSRMQGVLNSVVSENQSAFVKG 371
>BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [imported] -
Arabidopsis thaliana, partial (7%)
Length = 687
Score = 65.9 bits (159), Expect = 1e-11
Identities = 37/119 (31%), Positives = 59/119 (49%)
Frame = +2
Query: 99 EVAFTSEEIWEVVIACDGNRALGPYGFNFIFFREFWDIIKDDMMKLFVEFYEFGKLVEGL 158
E FT EE+ E + +A GP G +FF+++W I+ ++ ++ ++ E L
Sbjct: 248 EKEFTEEEVLEAINQMHPVKAPGPDGLPALFFQKYWHIVGKEVQQMVLQVLNNSMETEEL 427
Query: 159 NLKFIALIPKKKILEAVSVYRPISLTGSIYILIAKDLT*RFHMVMPYLLSWNQFSFTKG 217
N FI LIPK K YRPISL + +I K + R +P ++ Q +F +G
Sbjct: 428 NKTFIVLIPKGKNPNTPKDYRPISLCNVVMKIITKVIANRVKQTLPDVIDVEQSAFVQG 604
>BG587108 similar to PIR|G96509|G9 protein F27F5.21 [imported] - Arabidopsis
thaliana, partial (17%)
Length = 677
Score = 63.5 bits (153), Expect = 6e-11
Identities = 40/104 (38%), Positives = 53/104 (50%)
Frame = +3
Query: 114 CDGNRALGPYGFNFIFFREFWDIIKDDMMKLFVEFYEFGKLVEGLNLKFIALIPKKKILE 173
C + LG FF+ W IIK D++K+ F GKL LN I LIPKKK
Sbjct: 366 CTRKKLLGRME*QLSFFQHSWHIIKMDLLKMVNSFLASGKLDTRLNTTNICLIPKKKRPT 545
Query: 174 AVSVYRPISLTGSIYILIAKDLT*RFHMVMPYLLSWNQFSFTKG 217
++ RPISL Y +I+K L R + +P L+S Q +F G
Sbjct: 546 RMTELRPISLCNVGYKIISKVLCQRLKVCLPSLISETQSAFVHG 677
>TC90516
Length = 983
Score = 55.5 bits (132), Expect = 2e-08
Identities = 37/124 (29%), Positives = 63/124 (49%)
Frame = -2
Query: 94 DKSMLEVAFTSEEIWEVVIACDGNRALGPYGFNFIFFREFWDIIKDDMMKLFVEFYEFGK 153
D +L F ++ VV + DGN + P GFN FF +++K D+ +F +FY
Sbjct: 544 DNVLLSAQFLVSKMELVVSSLDGNESPRPDGFNLNFFIRLRNMLKADIEIMFEQFYTPAN 365
Query: 154 LVEGLNLKFIALIPKKKILEAVSVYRPISLTGSIYILIAKDLT*RFHMVMPYLLSWNQFS 213
L++ + F+ LI K + + + +S GS+ L+AK L R +M ++ NQ +
Sbjct: 364 LLKIFS*YFLTLISKVEYPILLGDFSLMSFLGSL*KLMAKVLALRLAHIMEKIIFVNQST 185
Query: 214 FTKG 217
F +G
Sbjct: 184 FVRG 173
>BG586267 weakly similar to PIR|A84888|A8 hypothetical protein At2g45230
[imported] - Arabidopsis thaliana, partial (16%)
Length = 794
Score = 55.1 bits (131), Expect = 2e-08
Identities = 26/70 (37%), Positives = 38/70 (54%)
Frame = +3
Query: 90 VKVEDKSMLEVAFTSEEIWEVVIACDGNRALGPYGFNFIFFREFWDIIKDDMMKLFVEFY 149
V E + L + EE+ E V + ++ GP G N FF++FWD + DD+ + EF
Sbjct: 375 VTEEQNAQLMAQISREEVREAVFDINPHKCPGPDGMNVFFFQQFWDTMGDDLTSMAQEFL 554
Query: 150 EFGKLVEGLN 159
GKL EG+N
Sbjct: 555 RTGKLEEGIN 584
Score = 36.2 bits (82), Expect = 0.010
Identities = 20/65 (30%), Positives = 35/65 (53%)
Frame = +1
Query: 150 EFGKLVEGLNLKFIALIPKKKILEAVSVYRPISLTGSIYILIAKDLT*RFHMVMPYLLSW 209
E G L + L+PKK + + +RPISL Y +++K L+ R V+P++++
Sbjct: 556 EQGNLKRESTKQTSGLVPKKLEAKRLVEFRPISLCNVAYKIVSKVLSKRLKSVLPWIITE 735
Query: 210 NQFSF 214
Q +F
Sbjct: 736 TQAAF 750
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 52.4 bits (124), Expect = 1e-07
Identities = 29/70 (41%), Positives = 44/70 (62%)
Frame = +2
Query: 147 EFYEFGKLVEGLNLKFIALIPKKKILEAVSVYRPISLTGSIYILIAKDLT*RFHMVMPYL 206
EF+ KL +G+N FIALIPK + ++ +RPISL GS+Y ++ K L R +V+ +
Sbjct: 17 EFHRNRKLFKGINSTFIALIPKVDNPQRLNDFRPISLVGSLYKILGKLLANRLRVVIGSV 196
Query: 207 LSWNQFSFTK 216
+S Q +F K
Sbjct: 197 ISDAQSAFVK 226
>AW736247 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (4%)
Length = 305
Score = 50.4 bits (119), Expect = 5e-07
Identities = 23/58 (39%), Positives = 38/58 (64%), Gaps = 2/58 (3%)
Frame = +1
Query: 102 FTSEEIWEVVIACDGNRA--LGPYGFNFIFFREFWDIIKDDMMKLFVEFYEFGKLVEG 157
F+ EE+ + V CD + + GP G NF F +E+W++IK D +++ +EF+ GKL +G
Sbjct: 121 FSEEEVRKAVWDCDSSHS*NPGPDGVNFTFVKEYWELIKVDFLRVVMEFHTNGKLGKG 294
>TC79660 similar to PIR|T05057|T05057 hypothetical protein M3E9.100 -
Arabidopsis thaliana, partial (85%)
Length = 1161
Score = 28.9 bits (63), Expect = 1.6
Identities = 17/45 (37%), Positives = 24/45 (52%)
Frame = +2
Query: 96 SMLEVAFTSEEIWEVVIACDGNRALGPYGFNFIFFREFWDIIKDD 140
S LE++F+ EE ++ ACD N LG FI ++KDD
Sbjct: 368 SKLEISFSEEETNDLFEACDINDDLGMKFSEFIVLLCLVYLLKDD 502
>TC90966 similar to GP|9758285|dbj|BAB08809.1 gene_id:MLE2.7~unknown protein
{Arabidopsis thaliana}, partial (87%)
Length = 896
Score = 28.1 bits (61), Expect = 2.7
Identities = 24/89 (26%), Positives = 43/89 (47%), Gaps = 2/89 (2%)
Frame = +2
Query: 94 DKSMLEVA--FTSEEIWEVVIACDGNRALGPYGFNFIFFREFWDIIKDDMMKLFVEFYEF 151
+KS L V T+ +++E ++ A+ P ++F W ++ M+ LF +
Sbjct: 602 NKSKLLVVEDLTARQVYEKLL-----EAVQP*CCPLLYFYYAWVLVSL*MLNLFHK---- 754
Query: 152 GKLVEGLNLKFIALIPKKKILEAVSVYRP 180
KLV GLN FI+ I + +A+ + P
Sbjct: 755 SKLVWGLNFSFISSILPRYTYKAIGLLHP 841
>AL380167 homologue to PIR|S55887|S558 CCHH finger protein 7 - Arabidopsis
thaliana, partial (11%)
Length = 476
Score = 27.7 bits (60), Expect = 3.5
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = -2
Query: 127 FIFFREFWDIIKDDMMKLFVEFYEFGKLVEGLNLK 161
F F + W + DM ++F ++ F K EG NLK
Sbjct: 148 FSFQVQIWSLHHVDMEQIFT*YFFFDKRKEGTNLK 44
>TC81941 similar to GP|15420162|gb|AAK97303.1 F-box containing protein ORE9
{Arabidopsis thaliana}, partial (35%)
Length = 1017
Score = 26.9 bits (58), Expect = 6.0
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = -3
Query: 158 LNLKFIALIPKKKILEAVSVYRPISLTGSIYILIAKDL 195
L+ KF L PK+KIL+ + +S+ +I I + K +
Sbjct: 694 LSQKFTNLYPKRKILQPIPAIAIVSIAVTIAIALHKQI 581
>TC77390 similar to GP|2462925|emb|CAA03884.1 GTP cyclohydrolase II / 3
4-dihydroxy-2-butanone-4-phoshate synthase {Arabidopsis
thaliana}, partial (74%)
Length = 2311
Score = 26.9 bits (58), Expect = 6.0
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +3
Query: 198 RFHMVMPYLLSWNQFSF 214
+ +++PYLL WNQ+ F
Sbjct: 1011 KLQLILPYLLVWNQWQF 1061
>TC78188 similar to PIR|C84870|C84870 probable splicing factor [imported] -
Arabidopsis thaliana, partial (95%)
Length = 1436
Score = 26.6 bits (57), Expect = 7.9
Identities = 13/51 (25%), Positives = 24/51 (46%), Gaps = 2/51 (3%)
Frame = -1
Query: 109 EVVIACDGNRALGPYGFNFIFFREFWDIIKDDM--MKLFVEFYEFGKLVEG 157
++ ++CD + P G NF+ + W + + + KL V + FG G
Sbjct: 389 KISLSCDPDATTDPVGLNFMVYTALWCPVSNMIGDSKLEVRCFPFGPGARG 237
>BG648169 similar to GP|8953386|emb| putative protein {Arabidopsis thaliana},
partial (4%)
Length = 635
Score = 26.6 bits (57), Expect = 7.9
Identities = 17/58 (29%), Positives = 34/58 (58%), Gaps = 2/58 (3%)
Frame = +1
Query: 153 KLVEGLNLKFIALIPKKKILEAVSVYRPISLTGSIYILIA--KDLT*RFHMVMPYLLS 208
KL++G++L+ +++PKKK+ AV P + +L+A + + R V+P++ S
Sbjct: 148 KLLKGIDLE--SILPKKKVRAAVLSIEPSEAFKLLEVLLAIWQSRSSRREYVLPWMYS 315
>TC89782 similar to GP|21618145|gb|AAM67195.1 unknown {Arabidopsis
thaliana}, partial (88%)
Length = 870
Score = 26.6 bits (57), Expect = 7.9
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +2
Query: 103 TSEEIWEVVIACDGNRALGPYGFNFIFFREFWDIIKDDMMKLFVEFYEFG 152
+ +EI E ++A N A PY FNF+ ++ D + + + EFG
Sbjct: 299 SEDEIKEKILANLANFAYDPYNFNFLRQLNVLELFLDCVTEPNEKIIEFG 448
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.346 0.159 0.520
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,084,903
Number of Sequences: 36976
Number of extensions: 72350
Number of successful extensions: 631
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 629
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 631
length of query: 217
length of database: 9,014,727
effective HSP length: 92
effective length of query: 125
effective length of database: 5,612,935
effective search space: 701616875
effective search space used: 701616875
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0173a.1