BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0172.11
(148 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG583240 homologue to GP|3608481|gb| ribosomal protein L27a {Pet... 268 6e-73
TC85887 similar to SP|P49637|R27C_ARATH 60S ribosomal protein L2... 260 2e-70
TC85888 similar to SP|P49637|R27C_ARATH 60S ribosomal protein L2... 259 4e-70
BQ154254 similar to SP|P49637|R27C_ 60S ribosomal protein L27a-C... 145 8e-36
BG454473 similar to GP|12329967|emb serine-threonine protein kin... 30 0.47
BF004258 similar to GP|4586596|dbj| fructose-bisphosphate aldola... 29 0.80
TC81693 28 1.0
TC81272 similar to PIR|T05225|T05225 extensin homolog F17I5.160 ... 28 1.8
AW585816 homologue to SP|P46257|ALF2 Fructose-bisphosphate aldol... 28 1.8
TC85308 homologue to SP|O65735|ALF_CICAR Fructose-bisphosphate a... 28 1.8
TC88014 similar to GP|15215786|gb|AAK91438.1 AT3g43520/T18D12_90... 27 3.0
TC92570 similar to GP|15290605|gb|AAK94907.1 seven transmembrane... 27 4.0
TC81307 weakly similar to GP|4335772|gb|AAD17449.1| unknown prot... 27 4.0
BQ135963 27 4.0
TC77084 similar to GP|15529155|gb|AAK97672.1 AT3g30390/T6J22_16 ... 26 6.8
AW587010 similar to PIR|E96613|E966 hypothetical protein T15M6.2... 25 8.8
TC88887 weakly similar to GP|6523547|emb|CAB62280.1 hydroxyproli... 25 8.8
TC84613 similar to GP|10176939|dbj|BAB10088. DNAJ-like protein {... 25 8.8
>BG583240 homologue to GP|3608481|gb| ribosomal protein L27a {Petunia x
hybrida}, partial (93%)
Length = 722
Score = 268 bits (685), Expect = 6e-73
Identities = 128/147 (87%), Positives = 134/147 (91%)
Frame = +3
Query: 2 TTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYF 61
TT KKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYF
Sbjct: 3 TTSLKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYF 182
Query: 62 HKLRNQFFCPTVNIDKLWSLLPQEVKDQAANDKSKAPVIDVTNYGYFKVLGKGVLPANQP 121
HKLRN+F PTVNID LWSLLPQEVKD+A+ K APVIDVT +G+FKVLGKGVLP NQ
Sbjct: 183 HKLRNKFHSPTVNIDTLWSLLPQEVKDKASKSKDVAPVIDVTQFGFFKVLGKGVLPKNQS 362
Query: 122 VVVKAKLISKIAEKKIKENGGAVLLTA 148
VVVKAKL+SKIAEKKIKE GGAVLLTA
Sbjct: 363 VVVKAKLVSKIAEKKIKEAGGAVLLTA 443
>TC85887 similar to SP|P49637|R27C_ARATH 60S ribosomal protein L27a-C.
[Mouse-ear cress] {Arabidopsis thaliana}, complete
Length = 736
Score = 260 bits (664), Expect = 2e-70
Identities = 124/148 (83%), Positives = 137/148 (91%)
Frame = +3
Query: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY
Sbjct: 54 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 233
Query: 61 FHKLRNQFFCPTVNIDKLWSLLPQEVKDQAANDKSKAPVIDVTNYGYFKVLGKGVLPANQ 120
FHKLRN+F+ P VNIDK+ SL+PQ+VK++A + +KAPVIDVT +G+FK+LGKGVLP NQ
Sbjct: 234 FHKLRNKFYNPIVNIDKIASLIPQDVKEKALKE-NKAPVIDVTQFGFFKLLGKGVLPQNQ 410
Query: 121 PVVVKAKLISKIAEKKIKENGGAVLLTA 148
P VVK KLISKIAEKKIKE GGAV+LTA
Sbjct: 411 PFVVKTKLISKIAEKKIKEAGGAVVLTA 494
>TC85888 similar to SP|P49637|R27C_ARATH 60S ribosomal protein L27a-C.
[Mouse-ear cress] {Arabidopsis thaliana}, complete
Length = 585
Score = 259 bits (661), Expect = 4e-70
Identities = 123/148 (83%), Positives = 137/148 (92%)
Frame = +1
Query: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY
Sbjct: 40 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 219
Query: 61 FHKLRNQFFCPTVNIDKLWSLLPQEVKDQAANDKSKAPVIDVTNYGYFKVLGKGVLPANQ 120
FHKLRN+F+ P VNIDK+ SL+PQ+VK++A + +KAP+IDVT +G+FK+LGKGVLP NQ
Sbjct: 220 FHKLRNKFYNPIVNIDKIASLIPQDVKEKALKE-NKAPMIDVTQFGFFKLLGKGVLPQNQ 396
Query: 121 PVVVKAKLISKIAEKKIKENGGAVLLTA 148
P VVK KLISKIAEKKIKE GGAV+LTA
Sbjct: 397 PFVVKTKLISKIAEKKIKEAGGAVVLTA 480
>BQ154254 similar to SP|P49637|R27C_ 60S ribosomal protein L27a-C. [Mouse-ear
cress] {Arabidopsis thaliana}, partial (64%)
Length = 516
Score = 145 bits (365), Expect = 8e-36
Identities = 72/97 (74%), Positives = 85/97 (87%)
Frame = +3
Query: 52 YFGKVGMRYFHKLRNQFFCPTVNIDKLWSLLPQEVKDQAANDKSKAPVIDVTNYGYFKVL 111
YFGKVGMRYFHKLRN+F+ P VNIDK+ SL+PQ+VK++A + +KAPVIDVT +G+FK+L
Sbjct: 9 YFGKVGMRYFHKLRNKFYNPIVNIDKIASLIPQDVKEKALKE-NKAPVIDVTQFGFFKLL 185
Query: 112 GKGVLPANQPVVVKAKLISKIAEKKIKENGGAVLLTA 148
GKGVLP NQP VVK KLISKIAE KIKE GGAV+LTA
Sbjct: 186 GKGVLPQNQPFVVKTKLISKIAEXKIKEAGGAVVLTA 296
>BG454473 similar to GP|12329967|emb serine-threonine protein kinase
{Leishmania major}, partial (3%)
Length = 638
Score = 29.6 bits (65), Expect = 0.47
Identities = 14/32 (43%), Positives = 19/32 (58%), Gaps = 3/32 (9%)
Frame = +1
Query: 11 KRGHVSAGHGRIGK---HRKHPGGRGNAGGMH 39
+R H AG + + HR H GGRG++GG H
Sbjct: 28 RRSHQCAGAQHLTEASHHRIHCGGRGSSGGQH 123
>BF004258 similar to GP|4586596|dbj| fructose-bisphosphate aldolase {Cicer
arietinum}, partial (45%)
Length = 229
Score = 28.9 bits (63), Expect = 0.80
Identities = 16/54 (29%), Positives = 28/54 (51%)
Frame = +1
Query: 91 ANDKSKAPVIDVTNYGYFKVLGKGVLPANQPVVVKAKLISKIAEKKIKENGGAV 144
+N KSKA + + Y GKG+L A++ K +S I++ ++ N A+
Sbjct: 28 SNFKSKAHDELIADAAYIGTPGKGILAADESTGTIGKRLSSISDDNVESNRRAL 189
>TC81693
Length = 1012
Score = 28.5 bits (62), Expect = 1.0
Identities = 15/35 (42%), Positives = 19/35 (53%)
Frame = -3
Query: 14 HVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKY 48
HV GHGR H KHP GN G + +++ KY
Sbjct: 530 HVYKGHGR---HNKHPWKTGNGG--NGSKMVLQKY 441
>TC81272 similar to PIR|T05225|T05225 extensin homolog F17I5.160 -
Arabidopsis thaliana, partial (3%)
Length = 757
Score = 27.7 bits (60), Expect = 1.8
Identities = 20/53 (37%), Positives = 24/53 (44%), Gaps = 7/53 (13%)
Frame = -2
Query: 20 GRIGKHRKHPG---GR----GNAGGMHHHRILFDKYHPGYFGKVGMRYFHKLR 65
GR G+HRKH G GR G A G H H+ G+ G R H+ R
Sbjct: 399 GRAGEHRKHQGRAEGRRKHQGRAEGRHWHQ-----------GRAGERQRHRDR 274
Score = 26.2 bits (56), Expect = 5.2
Identities = 16/56 (28%), Positives = 25/56 (44%), Gaps = 7/56 (12%)
Frame = -2
Query: 6 KKNRKKRGHVSAGHGRIGKHRKHPG-------GRGNAGGMHHHRILFDKYHPGYFG 54
+++R + G R G+HRKH G +G A HR ++H G +G
Sbjct: 291 QRHRDRAGEHRKRQDRAGEHRKHQGRAEEHRKHQGQAEEHRWHRGRAGEHHRGRWG 124
>AW585816 homologue to SP|P46257|ALF2 Fructose-bisphosphate aldolase
cytoplasmic isozyme 2 (EC 4.1.2.13). [Garden pea] {Pisum
sativum}, partial (54%)
Length = 614
Score = 27.7 bits (60), Expect = 1.8
Identities = 16/54 (29%), Positives = 27/54 (49%)
Frame = +2
Query: 91 ANDKSKAPVIDVTNYGYFKVLGKGVLPANQPVVVKAKLISKIAEKKIKENGGAV 144
+N KSK + N Y GKG+L A++ K +S I+ + ++ N A+
Sbjct: 23 SNFKSKYHDELIANAAYIGTPGKGILAADESTGTIGKRLSSISVENVESNRRAL 184
>TC85308 homologue to SP|O65735|ALF_CICAR Fructose-bisphosphate aldolase
cytoplasmic isozyme (EC 4.1.2.13). [Chickpea Garbanzo],
partial (98%)
Length = 1507
Score = 27.7 bits (60), Expect = 1.8
Identities = 16/54 (29%), Positives = 27/54 (49%)
Frame = +2
Query: 91 ANDKSKAPVIDVTNYGYFKVLGKGVLPANQPVVVKAKLISKIAEKKIKENGGAV 144
+N KSK + N Y GKG+L A++ K +S I+ + ++ N A+
Sbjct: 143 SNFKSKYHDELIANAAYIGTPGKGILAADESTGTIGKRLSSISVENVESNRRAL 304
>TC88014 similar to GP|15215786|gb|AAK91438.1 AT3g43520/T18D12_90
{Arabidopsis thaliana}, partial (44%)
Length = 1052
Score = 26.9 bits (58), Expect = 3.0
Identities = 19/65 (29%), Positives = 28/65 (42%)
Frame = -1
Query: 4 RFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRYFHK 63
RFK NR+++ + +G+ H HP N L+DK+ P + K FH
Sbjct: 944 RFKHNREQKRAMCVNNGQTKVHIYHPYFTTNV-------YLYDKHKPWVYSK--NPKFHN 792
Query: 64 LRNQF 68
R F
Sbjct: 791 KRGLF 777
>TC92570 similar to GP|15290605|gb|AAK94907.1 seven transmembrane protein
MLO2 {Oryza sativa} [Oryza sativa (indica
cultivar-group)], partial (47%)
Length = 944
Score = 26.6 bits (57), Expect = 4.0
Identities = 12/36 (33%), Positives = 17/36 (46%)
Frame = +3
Query: 41 HRILFDKYHPGYFGKVGMRYFHKLRNQFFCPTVNID 76
H+ F K H G+ K G+R+ QFF +D
Sbjct: 555 HQTTFVKRHSGWSEKPGIRWIVAFFRQFFASVSKVD 662
>TC81307 weakly similar to GP|4335772|gb|AAD17449.1| unknown protein
{Arabidopsis thaliana}, partial (31%)
Length = 1171
Score = 26.6 bits (57), Expect = 4.0
Identities = 13/24 (54%), Positives = 18/24 (74%)
Frame = -3
Query: 4 RFKKNRKKRGHVSAGHGRIGKHRK 27
R +KN+KKRG+ +G GR GK R+
Sbjct: 407 RERKNKKKRGNKRSGKGR-GKSRE 339
>BQ135963
Length = 716
Score = 26.6 bits (57), Expect = 4.0
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +2
Query: 60 YFHKLRNQFFCPTVNIDKLWSLL 82
Y+H R+++ CPT LW L+
Sbjct: 134 YYHGGRHKYICPTNTSPNLWELV 202
>TC77084 similar to GP|15529155|gb|AAK97672.1 AT3g30390/T6J22_16
{Arabidopsis thaliana}, partial (88%)
Length = 2353
Score = 25.8 bits (55), Expect = 6.8
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = -3
Query: 104 NYGYFKVLGKGVLPANQPVVVKAKL 128
N FKV GK ++PA P++V AKL
Sbjct: 563 NPNCFKVAGKAMIPA--PIIVVAKL 495
>AW587010 similar to PIR|E96613|E966 hypothetical protein T15M6.22 [imported]
- Arabidopsis thaliana, partial (3%)
Length = 542
Score = 25.4 bits (54), Expect = 8.8
Identities = 19/66 (28%), Positives = 28/66 (41%), Gaps = 12/66 (18%)
Frame = -1
Query: 28 HPGGRGNAGGMHHHR--ILFDKYHPGYF------GKVGMRYFHKLRNQF----FCPTVNI 75
HP G G+ HHH+ +L + GY + Y H+ +QF C T+N+
Sbjct: 500 HPMGHGSLPPSHHHQLHLLHASHQKGYSSSH*SPSSSPLLYHHQHLHQFPAIHLCLTMNL 321
Query: 76 DKLWSL 81
SL
Sbjct: 320 PHRGSL 303
>TC88887 weakly similar to GP|6523547|emb|CAB62280.1 hydroxyproline-rich
glycoprotein DZ-HRGP {Volvox carteri f. nagariensis},
partial (18%)
Length = 1681
Score = 25.4 bits (54), Expect = 8.8
Identities = 16/42 (38%), Positives = 17/42 (40%)
Frame = -2
Query: 16 SAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVG 57
+A G G PGG G GGM HPG FG G
Sbjct: 1101 TANRGGFGGGPGGPGGPGGPGGM----------HPGGFGGGG 1006
>TC84613 similar to GP|10176939|dbj|BAB10088. DNAJ-like protein {Arabidopsis
thaliana}, partial (10%)
Length = 628
Score = 25.4 bits (54), Expect = 8.8
Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 2/36 (5%)
Frame = +3
Query: 11 KRGHVSAGHGR--IGKHRKHPGGRGNAGGMHHHRIL 44
+R HV HG +G H H + GG HH +L
Sbjct: 147 RRNHVYRTHGGGGMGHHHHHQRPEVHGGGGHHLMLL 254
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.140 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,233,815
Number of Sequences: 36976
Number of extensions: 82490
Number of successful extensions: 406
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 396
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 399
length of query: 148
length of database: 9,014,727
effective HSP length: 87
effective length of query: 61
effective length of database: 5,797,815
effective search space: 353666715
effective search space used: 353666715
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0172.11