Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0171.3
         (96 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BI270896 similar to PIR|F96729|F967 probable alliinase F5A18.26 ...    67  1e-12
TC79770 weakly similar to GP|14334163|gb|AAK60564.1 armadillo re...    28  0.59
TC87549 similar to PIR|T01734|T01734 hypothetical protein A_IG00...    27  1.0
TC84107 similar to GP|22945215|gb|AAF50957.2 CG3047-PA {Drosophi...    27  1.3
TC79680 weakly similar to GP|21751020|dbj|BAC03887. unnamed prot...    27  1.7
TC78851 sucrose-phosphatase                                            26  2.2
BQ139251 similar to GP|396595|emb|C ACR1-protein {Saccharomyces ...    26  2.9
TC86716 similar to PIR|T14279|T14279 myosin-like protein my5 - c...    25  3.8
TC78295 similar to PIR|T48496|T48496 membrane protein - Arabidop...    25  3.8
AL377347 similar to PIR|T05452|T05 hypothetical protein F7K2.160...    25  3.8
BI266917 PIR|S01173|MC calmodulin [validated] - fruit fly (Droso...    25  5.0
BQ135422                                                               25  5.0
TC86588 similar to GP|5139814|dbj|BAA81736.1 GmMYB29B2 {Glycine ...    25  5.0
CA917294 similar to GP|6691680|dbj| Mga23 {Streptococcus pyogene...    25  5.0
CA859305                                                               25  6.5
TC87898 weakly similar to GP|1174199|gb|AAA86652.1| S25-PR6 {Nic...    24  8.5
TC82482 weakly similar to GP|15146210|gb|AAK83588.1 AT3g09210/F3...    24  8.5

>BI270896 similar to PIR|F96729|F967 probable alliinase F5A18.26 [imported] -
           Arabidopsis thaliana, partial (12%)
          Length = 608

 Score = 67.0 bits (162), Expect = 1e-12
 Identities = 41/96 (42%), Positives = 58/96 (59%)
 Frame = +1

Query: 1   MAKLPNVFSLRHLLVLSLALNVSFILRLLFEGEEGHDHCSCLRKERRAKIARADSNTETG 60
           MA  P + SLRHLLVLSLALNVS ILR+++EG++                 +  SN +  
Sbjct: 175 MANFPAMLSLRHLLVLSLALNVSLILRMVYEGDD-----------------QQGSNNQNM 303

Query: 61  EQSIKESRVAVSSSTSSLANSTCKDHPGGRNRVINL 96
            + I++SR+ + S+ S  ANST KDH G  +++INL
Sbjct: 304 GRIIQKSRLVIPSTIS--ANSTRKDHAGVMDKIINL 405


>TC79770 weakly similar to GP|14334163|gb|AAK60564.1 armadillo
           repeat-containing protein {Nicotiana tabacum}, partial
           (39%)
          Length = 1860

 Score = 28.1 bits (61), Expect = 0.59
 Identities = 15/44 (34%), Positives = 25/44 (56%)
 Frame = -2

Query: 42  LRKERRAKIARADSNTETGEQSIKESRVAVSSSTSSLANSTCKD 85
           L +E+R K + +DSN ETG   +  S   V +++  +  +TC D
Sbjct: 404 LTREKRLKTSSSDSNNETG---VCVSWEMVDAASCIIMGATCSD 282


>TC87549 similar to PIR|T01734|T01734 hypothetical protein A_IG002N01.30 -
            Arabidopsis thaliana (fragment), partial (38%)
          Length = 1766

 Score = 27.3 bits (59), Expect = 1.0
 Identities = 15/39 (38%), Positives = 25/39 (63%), Gaps = 5/39 (12%)
 Frame = -3

Query: 53   ADSNTETGEQS-----IKESRVAVSSSTSSLANSTCKDH 86
            A S++++G +S     I+ S ++ SSS+SSL  S+  DH
Sbjct: 1680 ASSSSKSGGESSSSSSIQSSSISSSSSSSSLIKSSASDH 1564


>TC84107 similar to GP|22945215|gb|AAF50957.2 CG3047-PA {Drosophila
           melanogaster}, partial (2%)
          Length = 522

 Score = 26.9 bits (58), Expect = 1.3
 Identities = 15/32 (46%), Positives = 17/32 (52%)
 Frame = -3

Query: 24  FILRLLFEGEEGHDHCSCLRKERRAKIARADS 55
           F LRL   GEEG D C C + +RR      DS
Sbjct: 319 FQLRLF--GEEGGDECRCTQDQRRR*EVEEDS 230


>TC79680 weakly similar to GP|21751020|dbj|BAC03887. unnamed protein product
            {Homo sapiens}, partial (13%)
          Length = 2636

 Score = 26.6 bits (57), Expect = 1.7
 Identities = 12/39 (30%), Positives = 20/39 (50%)
 Frame = -3

Query: 45   ERRAKIARADSNTETGEQSIKESRVAVSSSTSSLANSTC 83
            + ++K+     NT T EQS  +  V       SL++S+C
Sbjct: 1377 DAKSKVVGCSDNTSTTEQSESKVSVITLGDIDSLSHSSC 1261


>TC78851 sucrose-phosphatase
          Length = 1617

 Score = 26.2 bits (56), Expect = 2.2
 Identities = 19/82 (23%), Positives = 35/82 (42%), Gaps = 1/82 (1%)
 Frame = -2

Query: 3    KLPNVFSLR-HLLVLSLALNVSFILRLLFEGEEGHDHCSCLRKERRAKIARADSNTETGE 61
            +L N++  R H + L  AL   +I+   +     HD C+C++  +          T+   
Sbjct: 1481 ELHNIYCKRDHKIKLG*ALGTVYIIHSFWPDCSNHDWCTCIQVNQSESFLSMVVTTQPCR 1302

Query: 62   QSIKESRVAVSSSTSSLANSTC 83
             S   S   +S+ TS ++   C
Sbjct: 1301 SSSCNSH--LSNFTSHISELIC 1242


>BQ139251 similar to GP|396595|emb|C ACR1-protein {Saccharomyces cerevisiae},
           partial (24%)
          Length = 678

 Score = 25.8 bits (55), Expect = 2.9
 Identities = 9/20 (45%), Positives = 15/20 (75%)
 Frame = +3

Query: 34  EGHDHCSCLRKERRAKIARA 53
           E H+H SCL+ +R+  +AR+
Sbjct: 264 ELHNHTSCLQSQRKGLVARS 323


>TC86716 similar to PIR|T14279|T14279 myosin-like protein my5 - common
           sunflower, partial (38%)
          Length = 2675

 Score = 25.4 bits (54), Expect = 3.8
 Identities = 15/51 (29%), Positives = 27/51 (52%)
 Frame = +2

Query: 29  LFEGEEGHDHCSCLRKERRAKIARADSNTETGEQSIKESRVAVSSSTSSLA 79
           LF+  E  D  +   +E   ++    SN+E+  Q +++  +AVS +  SLA
Sbjct: 362 LFKKVEDSDRKADQLQELVQRLEEKISNSESENQVLRQQALAVSPTAKSLA 514


>TC78295 similar to PIR|T48496|T48496 membrane protein - Arabidopsis
           thaliana, partial (82%)
          Length = 1223

 Score = 25.4 bits (54), Expect = 3.8
 Identities = 13/30 (43%), Positives = 19/30 (63%), Gaps = 3/30 (10%)
 Frame = +1

Query: 13  LLVLSLALNVSFI---LRLLFEGEEGHDHC 39
           L V+SL L ++ +   L +L  GE G+DHC
Sbjct: 865 LWVVSLVLLIAGLCIGLVMLLRGESGYDHC 954


>AL377347 similar to PIR|T05452|T05 hypothetical protein F7K2.160 -
           Arabidopsis thaliana, partial (31%)
          Length = 462

 Score = 25.4 bits (54), Expect = 3.8
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = -1

Query: 5   PNVFSLRHLLVLSLALNVSFILRLLFEGEE 34
           PN F    LLVLSL+ ++  +LR+L   E+
Sbjct: 213 PNEFPFTLLLVLSLSHSILILLRMLGVAEK 124


>BI266917 PIR|S01173|MC calmodulin [validated] - fruit fly (Drosophila
           melanogaster), complete
          Length = 576

 Score = 25.0 bits (53), Expect = 5.0
 Identities = 13/35 (37%), Positives = 21/35 (59%)
 Frame = -1

Query: 1   MAKLPNVFSLRHLLVLSLALNVSFILRLLFEGEEG 35
           M  LP++ + R+   +S +L+VSFILR +     G
Sbjct: 312 MNPLPSLSNTRNASRISSSLSVSFILRAIIVRNSG 208


>BQ135422 
          Length = 779

 Score = 25.0 bits (53), Expect = 5.0
 Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 5/38 (13%)
 Frame = +2

Query: 11  RHLLVLSLA---LNVSFILRLLF--EGEEGHDHCSCLR 43
           RHLL  ++A   +N+ F  R  +   G   H HCSC R
Sbjct: 386 RHLLTFNIAD*IVNIPF*HRYSWIQRGATKHIHCSCYR 499


>TC86588 similar to GP|5139814|dbj|BAA81736.1 GmMYB29B2 {Glycine max},
           partial (85%)
          Length = 1389

 Score = 25.0 bits (53), Expect = 5.0
 Identities = 15/36 (41%), Positives = 23/36 (63%)
 Frame = +2

Query: 47  RAKIARADSNTETGEQSIKESRVAVSSSTSSLANST 82
           + KI R+DSN+ T  QS  ES  A ++S+S  ++ T
Sbjct: 494 KPKIKRSDSNSSTITQS--ESASACTTSSSDFSSVT 595


>CA917294 similar to GP|6691680|dbj| Mga23 {Streptococcus pyogenes}, partial
           (3%)
          Length = 662

 Score = 25.0 bits (53), Expect = 5.0
 Identities = 18/38 (47%), Positives = 23/38 (60%)
 Frame = -3

Query: 9   SLRHLLVLSLALNVSFILRLLFEGEEGHDHCSCLRKER 46
           S  HLL+L LAL +SF   L+F   EG +    L+KER
Sbjct: 135 SFLHLLLLLLAL-LSFFTHLIF--VEGDEF*VLLKKER 31


>CA859305 
          Length = 735

 Score = 24.6 bits (52), Expect = 6.5
 Identities = 17/64 (26%), Positives = 30/64 (46%)
 Frame = +3

Query: 12  HLLVLSLALNVSFILRLLFEGEEGHDHCSCLRKERRAKIARADSNTETGEQSIKESRVAV 71
           + L+L + ++ S  L  +     G D C+    E   K   A + + TG+ +I  S+   
Sbjct: 327 YFLILLITISSSPFLLFVSSKPNGADTCNV--SEMATKGHGASTTSGTGQYTITTSKSTT 500

Query: 72  SSST 75
           SSS+
Sbjct: 501 SSSS 512


>TC87898 weakly similar to GP|1174199|gb|AAA86652.1| S25-PR6 {Nicotiana
           tabacum}, partial (25%)
          Length = 1300

 Score = 24.3 bits (51), Expect = 8.5
 Identities = 11/25 (44%), Positives = 14/25 (56%)
 Frame = -2

Query: 71  VSSSTSSLANSTCKDHPGGRNRVIN 95
           V S   S  +STC  H GG + V+N
Sbjct: 237 VISERISTYHSTCSVHLGGHHHVVN 163


>TC82482 weakly similar to GP|15146210|gb|AAK83588.1 AT3g09210/F3L24_8
           {Arabidopsis thaliana}, partial (17%)
          Length = 672

 Score = 24.3 bits (51), Expect = 8.5
 Identities = 10/24 (41%), Positives = 18/24 (74%)
 Frame = +3

Query: 10  LRHLLVLSLALNVSFILRLLFEGE 33
           +RH+LVL++ +   ++L L FEG+
Sbjct: 480 VRHVLVLNIEI*DVYLLSLKFEGD 551


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.317    0.131    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,054,678
Number of Sequences: 36976
Number of extensions: 34520
Number of successful extensions: 248
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 247
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 247
length of query: 96
length of database: 9,014,727
effective HSP length: 72
effective length of query: 24
effective length of database: 6,352,455
effective search space: 152458920
effective search space used: 152458920
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)


Lotus: description of TM0171.3