BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0162b.4
(228 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC78256 similar to GP|11385443|gb|AAG34804.1 glutathione S-trans... 351 1e-97
TC92451 similar to GP|11385449|gb|AAG34807.1 glutathione S-trans... 317 2e-87
TC87630 similar to GP|11385453|gb|AAG34809.1 glutathione S-trans... 242 1e-64
TC78052 similar to GP|11385441|gb|AAG34803.1 glutathione S-trans... 226 4e-60
TC90199 similar to GP|11385429|gb|AAG34797.1 glutathione S-trans... 213 4e-56
TC77359 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-t... 213 5e-56
TC85832 similar to GP|11385431|gb|AAG34798.1 glutathione S-trans... 212 1e-55
TC85833 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-t... 206 5e-54
TC86885 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-t... 205 1e-53
TC77358 similar to GP|11385429|gb|AAG34797.1 glutathione S-trans... 199 9e-52
TC93261 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-t... 199 9e-52
BQ146269 weakly similar to GP|11385445|gb glutathione S-transfer... 189 1e-48
TC81299 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-t... 172 2e-48
TC77361 similar to GP|11385429|gb|AAG34797.1 glutathione S-trans... 187 4e-48
TC79559 similar to GP|11385437|gb|AAG34801.1 glutathione S-trans... 187 4e-48
TC85907 similar to GP|11385445|gb|AAG34805.1 glutathione S-trans... 186 5e-48
TC77360 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-t... 171 6e-48
TC79139 weakly similar to GP|11385439|gb|AAG34802.1 glutathione ... 184 2e-47
TC77968 similar to GP|11385437|gb|AAG34801.1 glutathione S-trans... 181 2e-46
TC85908 similar to GP|11385445|gb|AAG34805.1 glutathione S-trans... 177 2e-45
>TC78256 similar to GP|11385443|gb|AAG34804.1 glutathione S-transferase GST
14 {Glycine max}, partial (96%)
Length = 883
Score = 351 bits (901), Expect = 1e-97
Identities = 168/228 (73%), Positives = 192/228 (83%)
Frame = +3
Query: 1 MGSQDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVH 60
MGSQDVKL+ FW SPF KRVEWALKLKGV YEYVEEDIFNK+ LLLELNPVHKK+PVLVH
Sbjct: 39 MGSQDVKLVGFWVSPFVKRVEWALKLKGVNYEYVEEDIFNKTPLLLELNPVHKKVPVLVH 218
Query: 61 GHKTIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGDE 120
K IAESFII+EYIDETWKQ PLLP PYERALARFWA+F EQKLL ++AMC +G+E
Sbjct: 219 AQKPIAESFIILEYIDETWKQSPLLPQHPYERALARFWADFAEQKLLNAAWIAMCNSGEE 398
Query: 121 QEKALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLK 180
QEKAL AREA+EKIEE I+GK+FFGG+NIGYLD+A+GWISYW+ ++EEVGS+ IIDPLK
Sbjct: 399 QEKALKVAREAIEKIEEEIKGKKFFGGDNIGYLDLALGWISYWLPVFEEVGSMQIIDPLK 578
Query: 181 FPATTAWMTNFLSHPVIKDTLPPRDKMIDYFHGRKKDLSETFRGRFKV 228
A TAWMTNFL+ PVI D+LPPRDKM+ YF R+ E F G FK+
Sbjct: 579 CSAITAWMTNFLNQPVINDSLPPRDKMLVYFQSRR----EAFHGLFKI 710
>TC92451 similar to GP|11385449|gb|AAG34807.1 glutathione S-transferase GST
17 {Glycine max}, partial (86%)
Length = 859
Score = 317 bits (813), Expect = 2e-87
Identities = 148/224 (66%), Positives = 185/224 (82%)
Frame = +2
Query: 1 MGSQDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVH 60
MG+QDVKLL+F SP +RVEWALKLKGVE++Y+EEDIFNKSSLLLE+NPVHKK+PVLVH
Sbjct: 29 MGNQDVKLLNFLLSPVGRRVEWALKLKGVEFDYIEEDIFNKSSLLLEMNPVHKKVPVLVH 208
Query: 61 GHKTIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGDE 120
G K+IAES II+EYIDETWKQYPLLP DPY+R+ ARFWA +++KL ++A+ G+E
Sbjct: 209 GQKSIAESLIILEYIDETWKQYPLLPPDPYQRSRARFWAKLSDEKLALGSWIALIKKGNE 388
Query: 121 QEKALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLK 180
EKAL EARE MEK+EE I+GK+FFGG+ IGYLD+ +GWI+ ++ IWEE+GS I+DPLK
Sbjct: 389 WEKALKEAREIMEKLEEDIKGKKFFGGDTIGYLDLTLGWITCFLPIWEEIGSTQILDPLK 568
Query: 181 FPATTAWMTNFLSHPVIKDTLPPRDKMIDYFHGRKKDLSETFRG 224
P+ ++W NFLSHP+IK+ LPPRD+MI Y H R K+ S T RG
Sbjct: 569 CPSISSWKINFLSHPIIKECLPPRDEMILYCHRRIKEYSSTHRG 700
>TC87630 similar to GP|11385453|gb|AAG34809.1 glutathione S-transferase GST
19 {Glycine max}, partial (98%)
Length = 967
Score = 242 bits (617), Expect = 1e-64
Identities = 111/207 (53%), Positives = 156/207 (74%)
Frame = +2
Query: 5 DVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHKT 64
+VKL++ S R+EWAL++KGVEYEY++ED+ NKS LLLE NPV+KK+PVL+H K+
Sbjct: 86 EVKLIATPQSFPCARIEWALRIKGVEYEYIQEDLLNKSPLLLESNPVYKKVPVLLHDGKS 265
Query: 65 IAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGDEQEKA 124
IAES +I+EYIDETWK PLLP DPYE+ALARFWA F ++K + V+ A G+E+ KA
Sbjct: 266 IAESLVILEYIDETWKDNPLLPLDPYEKALARFWAKFIDEKCIFGVWGACVAQGEEKAKA 445
Query: 125 LNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFPAT 184
++ A E++ +E+ I+GK+FFGGE IGYLDI GWISYW+++ EE+G + +I+ KFP+
Sbjct: 446 VDAAIESLAFLEKQIQGKKFFGGEKIGYLDIVAGWISYWLNVLEELGEMELINAEKFPSL 625
Query: 185 TAWMTNFLSHPVIKDTLPPRDKMIDYF 211
W NF+ IKD +PP++ +++YF
Sbjct: 626 HEWGHNFIQTSPIKDCIPPKEMVVEYF 706
>TC78052 similar to GP|11385441|gb|AAG34803.1 glutathione S-transferase GST
13 {Glycine max}, partial (97%)
Length = 1049
Score = 226 bits (577), Expect = 4e-60
Identities = 105/208 (50%), Positives = 147/208 (70%)
Frame = +3
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
++V+LL W SPFS R++ ALKLKGV Y+Y EE++ NKS LL+ NPV+KK+P+LVH
Sbjct: 165 EEVRLLGKWASPFSNRIDLALKLKGVPYKYSEENLANKSDDLLKYNPVYKKVPILVHNGN 344
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGDEQEK 123
IAES II+EYIDETWK PLL DPYERALARFW+ + K+L ++ A +EK
Sbjct: 345 PIAESLIILEYIDETWKNNPLLSEDPYERALARFWSKTLDDKILPAIWNACWSDEKGREK 524
Query: 124 ALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFPA 183
A+ EA EA++ +E+ I+ K+FFGGE +G +DIA +I YW++I +E+ + ++ KFP
Sbjct: 525 AVEEALEALKILEDEIKDKKFFGGETLGLVDIAANFIGYWVYILQEIAGLELLTLEKFPK 704
Query: 184 TTAWMTNFLSHPVIKDTLPPRDKMIDYF 211
W F++HPVIK+ LPPRD++ +F
Sbjct: 705 VYKWSQEFINHPVIKEGLPPRDELFAFF 788
>TC90199 similar to GP|11385429|gb|AAG34797.1 glutathione S-transferase GST
7 {Glycine max}, partial (90%)
Length = 785
Score = 213 bits (543), Expect = 4e-56
Identities = 97/216 (44%), Positives = 147/216 (67%), Gaps = 1/216 (0%)
Frame = +2
Query: 5 DVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHKT 64
+V+LL SPF +RV+ ALKLKG+EY Y+EE++ NKS LLL+ NP+HKK+PV VH K
Sbjct: 71 EVQLLGVGGSPFVRRVQIALKLKGIEYTYIEENLRNKSDLLLKYNPIHKKVPVFVHNGKP 250
Query: 65 IAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVF-VAMCITGDEQEK 123
+AES +I+EYIDETW+ YP+ P PYE+ALARFW+ F + K+L + A + +E+EK
Sbjct: 251 LAESLVILEYIDETWENYPIFPQQPYEKALARFWSRFIDDKILPAISKAAWTVNKEEREK 430
Query: 124 ALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFPA 183
+ E+ +A++ +E ++ K FFGGENI +DIA ++++W+ EE + ++ KFP
Sbjct: 431 GIEESLQALQFLENELKHK-FFGGENISIVDIAASYLAFWLPAIEEAVGLKVLSSDKFPK 607
Query: 184 TTAWMTNFLSHPVIKDTLPPRDKMIDYFHGRKKDLS 219
W F +HP++K+ LPP+D ++ +F R +S
Sbjct: 608 LYKWSQEFNNHPIVKENLPPKDGLLGFFKSRYAAIS 715
>TC77359 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-transferase
(EC 2.5.1.18) (Heat shock protein 26A) (G2-4).
[Soybean], complete
Length = 1034
Score = 213 bits (542), Expect = 5e-56
Identities = 103/220 (46%), Positives = 152/220 (68%), Gaps = 2/220 (0%)
Frame = +3
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
++VKLL SPF RV+ ALKLKG+EY+++EE++ NKS LLL+ NPV+KKIPV VH K
Sbjct: 51 EEVKLLGAIGSPFVCRVQIALKLKGIEYKFLEENMANKSELLLKYNPVYKKIPVFVHNEK 230
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGDEQEK 123
I+ES +IIEYIDETWKQ P+LP DPY+RALARFW+NF + K++ A+ DE+E+
Sbjct: 231 PISESLLIIEYIDETWKQNPILPSDPYQRALARFWSNFIDDKIIAASIKAVHTVHDEKER 410
Query: 124 ALN--EAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKF 181
N EA++ +E ++ K FFGGE IG +DIA +I++WI + +E+ + +++ K
Sbjct: 411 EKNVEGTIEALQILENELKDK-FFGGEEIGIVDIAAVYIAFWIPLIQEITGLQLLNAEKC 587
Query: 182 PATTAWMTNFLSHPVIKDTLPPRDKMIDYFHGRKKDLSET 221
P + W FL+HP++K+ +PPR+ + YF + L ++
Sbjct: 588 PKLSKWSQEFLNHPIVKENMPPREPLFAYFKAHYESLFDS 707
>TC85832 similar to GP|11385431|gb|AAG34798.1 glutathione S-transferase GST
8 {Glycine max}, partial (97%)
Length = 906
Score = 212 bits (539), Expect = 1e-55
Identities = 100/216 (46%), Positives = 147/216 (67%), Gaps = 1/216 (0%)
Frame = +1
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
+ VKLL SPF RV+ ALKLKG+EYE+VEE++ KS LL+ NPVHKK+PV VH K
Sbjct: 58 EHVKLLGATGSPFVCRVQIALKLKGIEYEFVEENLATKSEQLLKYNPVHKKVPVFVHNEK 237
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAM-CITGDEQE 122
I+ES +I+EYIDE WKQ P+LP DP++RALARFW+ F + K++ T F ++ + E+E
Sbjct: 238 PISESLVILEYIDEVWKQNPILPSDPHQRALARFWSKFIDDKIVSTSFKSVFSLDEKERE 417
Query: 123 KALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFP 182
K + EA EA+ +E ++ K +FGGE ++DIA ++++W+ + +++ + + KFP
Sbjct: 418 KNIEEATEALHFLENELKDK-YFGGEEFNFVDIAAVFVAFWVPLVQDITELQLFTAEKFP 594
Query: 183 ATTAWMTNFLSHPVIKDTLPPRDKMIDYFHGRKKDL 218
W FL HPV+K+TLPPR+ + +F GR + L
Sbjct: 595 KLYNWSQEFLDHPVVKETLPPREPLFAFFKGRYESL 702
>TC85833 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-transferase
(EC 2.5.1.18) (Heat shock protein 26A) (G2-4).
[Soybean], partial (98%)
Length = 928
Score = 206 bits (525), Expect = 5e-54
Identities = 97/216 (44%), Positives = 147/216 (67%), Gaps = 1/216 (0%)
Frame = +1
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
+ VKLL SPF RV+ ALKLKG+EYE++EE++ KS LLL+ NPVHKK+PV VH K
Sbjct: 28 EHVKLLGATGSPFVCRVQIALKLKGIEYEFLEENLATKSELLLKYNPVHKKVPVFVHNDK 207
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAM-CITGDEQE 122
I+ES +I+EYIDE WKQ P+LP DP++RALARFW+ F + K++ ++ + E+E
Sbjct: 208 PISESLVILEYIDEVWKQNPILPTDPHQRALARFWSKFIDDKIVSASSKSVFTLDEKERE 387
Query: 123 KALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFP 182
K + E EA++ ++ ++GK +FGGE ++DIA ++++WI + +++ + + KFP
Sbjct: 388 KNVEETTEALQFLQNELKGK-YFGGEEFNFVDIAAVFVAFWIPLVQDIIGLQLFTAEKFP 564
Query: 183 ATTAWMTNFLSHPVIKDTLPPRDKMIDYFHGRKKDL 218
W FL HPV+K+T+PPR+ + +F GR + L
Sbjct: 565 KLYNWSQEFLDHPVVKETIPPREPLFAFFKGRYESL 672
>TC86885 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-transferase
(EC 2.5.1.18) (Heat shock protein 26A) (G2-4).
[Soybean], complete
Length = 912
Score = 205 bits (522), Expect = 1e-53
Identities = 102/216 (47%), Positives = 140/216 (64%), Gaps = 1/216 (0%)
Frame = +2
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
++VKL SPF RVE ALKLKGVEY+Y E N S L + NP++KK+PVLVH K
Sbjct: 53 ENVKLFGMMASPFVSRVEIALKLKGVEYKYELEKGGNLSDTLKKYNPIYKKVPVLVHNDK 232
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETV-FVAMCITGDEQE 122
I+ES +I+EY+DETWKQ P+LP DPY+RALARFW F + K L A I E+E
Sbjct: 233 PISESLVILEYVDETWKQNPILPSDPYKRALARFWTKFIDDKCLSAARKAAFTIDEKERE 412
Query: 123 KALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFP 182
KA+ E EA + +E ++ + FFGGE IG +DIA +I++W I +E ++++ KFP
Sbjct: 413 KAIEETEEAFQVLENELK-ETFFGGEEIGIVDIAAVFIAFWFPIVQEATNLNLFTSEKFP 589
Query: 183 ATTAWMTNFLSHPVIKDTLPPRDKMIDYFHGRKKDL 218
W +F +HP++KD LPPR+ ++ YF R + L
Sbjct: 590 KLYKWSQDFTNHPIVKDKLPPREGLLTYFKARYESL 697
>TC77358 similar to GP|11385429|gb|AAG34797.1 glutathione S-transferase GST
7 {Glycine max}, partial (89%)
Length = 700
Score = 199 bits (505), Expect = 9e-52
Identities = 96/211 (45%), Positives = 140/211 (65%), Gaps = 1/211 (0%)
Frame = +3
Query: 1 MGSQDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVH 60
+ ++VKL SPFS RVE ALKLKGVEY Y EE + N S +++ NPV+KK+PVLVH
Sbjct: 69 LNQEEVKLFGLVGSPFSTRVEIALKLKGVEYRYEEEKVGNLSDTVIKYNPVYKKVPVLVH 248
Query: 61 GHKTIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVF-VAMCITGD 119
K IAES +IIEYIDETWKQ P+LP DPY+RALARFW+ F + K + + A +
Sbjct: 249 NDKPIAESLVIIEYIDETWKQNPILPSDPYKRALARFWSKFIDDKCMIPAWKTAFTLDEK 428
Query: 120 EQEKALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPL 179
E+EK+ E +A + +E ++ K FFGG++IG +DI +I++W I +EV + +
Sbjct: 429 EREKSFEETEDAFQFLENELKDK-FFGGDDIGLIDITAVFIAFWFPIVQEVTGLKLFTSE 605
Query: 180 KFPATTAWMTNFLSHPVIKDTLPPRDKMIDY 210
KFP W +F +HP++K+ LP +++++ +
Sbjct: 606 KFPKLYNWSQDFNNHPIVKENLPRKERLLAF 698
>TC93261 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-transferase
(EC 2.5.1.18) (Heat shock protein 26A) (G2-4).
[Soybean], partial (90%)
Length = 674
Score = 199 bits (505), Expect = 9e-52
Identities = 95/210 (45%), Positives = 141/210 (66%), Gaps = 2/210 (0%)
Frame = +3
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
+DVKLL SPF RV+ ALK KG+EYE+V+ED+ NKS LLL+ NPVHKKIPVLVH K
Sbjct: 45 EDVKLLGVVGSPFFCRVQIALKFKGIEYEFVKEDLTNKSDLLLKYNPVHKKIPVLVHNEK 224
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGDEQEK 123
I+ES +I+EYIDE WKQ P+L DPY+++LARFW+ F + K++ + F A+ DE+E+
Sbjct: 225 PISESLVILEYIDEVWKQNPILSSDPYQKSLARFWSKFIDDKIVTSSFKAVYAVNDEKER 404
Query: 124 ALN--EAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKF 181
N E+ EA++ +E ++ K +FGGE I ++DIA +I++WI + +++ + + + K+
Sbjct: 405 QKNVEESTEALQILENELKNK-YFGGEEINFVDIAAVFIAFWIPLIQDIAELKLFNAEKY 581
Query: 182 PATTAWMTNFLSHPVIKDTLPPRDKMIDYF 211
P W FL+HP + +P +F
Sbjct: 582 PKLYKWSQEFLNHPAXXEHVPAXXPXFAFF 671
>BQ146269 weakly similar to GP|11385445|gb glutathione S-transferase GST 15
{Glycine max}, partial (47%)
Length = 662
Score = 189 bits (479), Expect = 1e-48
Identities = 97/216 (44%), Positives = 132/216 (60%), Gaps = 6/216 (2%)
Frame = +1
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
+DVK+ W SP+++RV W LKLKGV YEY ED+ NKSSLLL+ NP++KK+PVLVH K
Sbjct: 19 EDVKVFGLWSSPYTQRVVWTLKLKGVSYEYYGEDLANKSSLLLQYNPIYKKVPVLVHNGK 198
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGDEQEK 123
+AES +I+EYIDETW +PLLP D YER+ RFW+ F E+K + + G+ QE+
Sbjct: 199 PMAESMVIVEYIDETWPPFPLLPQDAYERSNVRFWSKFIEEKSMH-MMEFFLYDGERQER 375
Query: 124 ALNEAREAMEKIEEV--IEGKRFFGGENIGYLDIAVGWISYWI----HIWEEVGSIHIID 177
A+ E E + IE+ + K F GG IG DIA+GW + W E SI I
Sbjct: 376 AIKETLETLRVIEKESGLXEKMFIGGNTIGLADIALGWGGTYSASHGRNWLESSSITID- 552
Query: 178 PLKFPATTAWMTNFLSHPVIKDTLPPRDKMIDYFHG 213
FP +W+ NFL + K+ L P + +++Y G
Sbjct: 553 --AFPHLHSWVKNFLGNSCYKNNLXPHELLVEYLQG 654
>TC81299 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-transferase
(EC 2.5.1.18) (Heat shock protein 26A) (G2-4).
[Soybean], partial (82%)
Length = 953
Score = 172 bits (436), Expect(2) = 2e-48
Identities = 91/181 (50%), Positives = 122/181 (67%), Gaps = 2/181 (1%)
Frame = +1
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
++VK+ SPFS RVE ALKLKGVEY+ E N S L NPV+KK+PVLV+ K
Sbjct: 166 EEVKVFGMVGSPFSTRVEIALKLKGVEYKCENEKFGNLSDTLKNYNPVYKKVPVLVYNDK 345
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGD--EQ 121
I+ES +I+EYIDETWKQ P+LP DPY+RA+ARFW+NF + K + V A C T D E+
Sbjct: 346 PISESLVILEYIDETWKQNPILPSDPYQRAMARFWSNFIDDKCMNDVRKA-CFTFDEKER 522
Query: 122 EKALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKF 181
EK++ E +EA+ +E ++ K FFGGE+IG++DIA IS+WI EE + + +F
Sbjct: 523 EKSIEETKEALRFLENELKDK-FFGGEDIGFVDIAAVIISFWISPIEEAFGLKLFTSEEF 699
Query: 182 P 182
P
Sbjct: 700 P 702
Score = 37.4 bits (85), Expect(2) = 2e-48
Identities = 12/37 (32%), Positives = 24/37 (64%)
Frame = +2
Query: 181 FPATTAWMTNFLSHPVIKDTLPPRDKMIDYFHGRKKD 217
FP+ W F +HP++ + LPPR+ ++ ++ GR ++
Sbjct: 698 FPSFYNWSQEFNNHPIVNEKLPPREILLTFYKGRYEE 808
>TC77361 similar to GP|11385429|gb|AAG34797.1 glutathione S-transferase GST
7 {Glycine max}, partial (88%)
Length = 715
Score = 187 bits (474), Expect = 4e-48
Identities = 92/208 (44%), Positives = 135/208 (64%), Gaps = 1/208 (0%)
Frame = +3
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
++VK+ SPF RVE ALKLKGVEY+Y +E + N S L + NPV+KK+PV +H K
Sbjct: 72 EEVKVFGLVESPFVTRVEIALKLKGVEYKYEQEKVGNLSETLKKYNPVYKKVPVFIHNDK 251
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETV-FVAMCITGDEQE 122
I+ES +I+EYIDETWKQ P+LP DP +RALARFW+NF + K V + E+E
Sbjct: 252 PISESLVILEYIDETWKQNPILPSDPSKRALARFWSNFIDDKCSNGARKVCFPLDEKERE 431
Query: 123 KALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFP 182
K++ E +EA+ +E ++ K FFGGE+IG++DIA IS+W +E + + +FP
Sbjct: 432 KSIEETKEALHFLENELKDK-FFGGEDIGFVDIAAVVISFWFSPIQEAFGLKLFTIEEFP 608
Query: 183 ATTAWMTNFLSHPVIKDTLPPRDKMIDY 210
W F +HP++K+ LPP++ ++ +
Sbjct: 609 KLYNWSQEFNNHPIVKEKLPPKETLLAF 692
>TC79559 similar to GP|11385437|gb|AAG34801.1 glutathione S-transferase GST
11 {Glycine max}, partial (78%)
Length = 775
Score = 187 bits (474), Expect = 4e-48
Identities = 98/208 (47%), Positives = 135/208 (64%), Gaps = 2/208 (0%)
Frame = +1
Query: 14 SPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHKTIAESFIIIE 73
SPF RVE ALKLKGVEY+Y E N S L+ NPV+KK+PVLVH K IAES +IIE
Sbjct: 13 SPFVCRVEIALKLKGVEYKYEVEKFQNLSDTLINYNPVYKKVPVLVHNDKPIAESLVIIE 192
Query: 74 YIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVF-VAMCITGDEQEKALNEAREAM 132
YIDETWKQ P+LP DP +RALARFW+ F + K L+ + V+ + E EK + E A+
Sbjct: 193 YIDETWKQNPILPSDPSKRALARFWSKFIDDKWLDAIIKVSSTLDEKEIEKGIEETEVAL 372
Query: 133 EKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFPATTAWMTNFL 192
+ +E ++ K FFGGE IG +DI +IS+W+ I +EV + + KFP W +F
Sbjct: 373 QFLEGELKDK-FFGGEEIGLVDITGVFISFWLPIVQEVIGLKLFTSEKFPKLYNWSQDFN 549
Query: 193 SHPVIKDTLPPR-DKMIDYFHGRKKDLS 219
+HP++K+ LPPR + ++ + R + L+
Sbjct: 550 NHPIVKEILPPRLETLLAFERSRSESLA 633
>TC85907 similar to GP|11385445|gb|AAG34805.1 glutathione S-transferase GST
15 {Glycine max}, partial (72%)
Length = 1156
Score = 186 bits (473), Expect = 5e-48
Identities = 92/210 (43%), Positives = 129/210 (60%), Gaps = 2/210 (0%)
Frame = +3
Query: 5 DVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHKT 64
+VKL FW SP++ RV W L LKG+ YE +EED FNKS LLL+ NP+HKK PVLVH K
Sbjct: 21 EVKLHGFWYSPYTLRVVWTLNLKGIPYENIEEDRFNKSPLLLQYNPIHKKTPVLVHDGKP 200
Query: 65 IAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGDEQEKA 124
I ES II+EYIDE W Q LLP DPY+RA ARFW + ++ + G+E+EKA
Sbjct: 201 ICESMIIVEYIDEIWPQNTLLPADPYDRAQARFWVKYVDELISVIAAFLHSKVGEEREKA 380
Query: 125 LNEAREAMEKIEE--VIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFP 182
E + IE+ + + K F+GG+ I +DIA+G +I + E++ + I+ +FP
Sbjct: 381 RENLWERLRVIEDQCLDDRKTFYGGDTINIVDIALGSFIKFIEVQEDMFKVKILQSERFP 560
Query: 183 ATTAWMTNFLSHPVIKDTLPPRDKMIDYFH 212
W NF PVIK+ P ++K++ + +
Sbjct: 561 RLHLWFNNFKDAPVIKENTPDQEKLVAFLN 650
>TC77360 similar to SP|P32110|GTX6_SOYBN Probable glutathione S-transferase
(EC 2.5.1.18) (Heat shock protein 26A) (G2-4).
[Soybean], complete
Length = 1140
Score = 171 bits (432), Expect(2) = 6e-48
Identities = 89/175 (50%), Positives = 119/175 (67%), Gaps = 2/175 (1%)
Frame = +3
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
++VK+ SPF RVE ALKLKGVEY Y +E N S L + NPV+KK+PV +H K
Sbjct: 51 EEVKVFGLVESPFVTRVEIALKLKGVEYIYEKEKFGNLSDTLKKYNPVYKKVPVFIHNDK 230
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGD--EQ 121
IAES +I+EYIDETWKQ P+LP DPY+RAL RFW+N + K L V A C T D E+
Sbjct: 231 PIAESLVILEYIDETWKQNPILPSDPYQRALVRFWSNLIDDKCLSGVRKA-CFTLDEKER 407
Query: 122 EKALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHII 176
EK++ E +EA++ +E ++ K FFGGE IG++DIA I++WI I +E + I+
Sbjct: 408 EKSIEETKEALQFLENELKDK-FFGGEEIGFVDIAAVLIAFWIPIIQEAFGLKIV 569
Score = 37.0 bits (84), Expect(2) = 6e-48
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = +1
Query: 180 KFPATTAWMTNFLSHPVIKDTLPPRDKMIDYFHGR 214
+FP W F +HP++K+ LPPR+ ++ ++ R
Sbjct: 580 EFPKLYNWSQEFNNHPIVKEKLPPRETLLAFYKAR 684
>TC79139 weakly similar to GP|11385439|gb|AAG34802.1 glutathione
S-transferase GST 12 {Glycine max}, partial (40%)
Length = 942
Score = 184 bits (468), Expect = 2e-47
Identities = 92/212 (43%), Positives = 131/212 (61%), Gaps = 6/212 (2%)
Frame = +2
Query: 3 SQDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGH 62
+ +V LL W S + +VE ALK+KG+ Y+YV+ED+ NKS LL+ NPVHKK+P+L+H
Sbjct: 5 TSEVVLLGNWASSYCTKVELALKVKGIPYKYVDEDLRNKSDSLLKYNPVHKKVPILLHKG 184
Query: 63 KTIAESFIIIEYIDETWKQYP-LLPHDPYERALARFWANFTEQKLLETVFVAMCITGDEQ 121
++I ES II+EYIDE W P LLP DPY+RA RFWAN+ +QK++ + + G+E+
Sbjct: 185 RSICESQIILEYIDEIWDHSPKLLPEDPYQRAKVRFWANYFDQKIIPSSYRIFQSRGEER 364
Query: 122 EKALNEAREAMEKIEEVI-----EGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHII 176
+KA E E + EE + E FF GE +G LDI V S ++E I +
Sbjct: 365 KKAAEEMEEILVVFEEGVKEGFNEKFHFFNGETLGLLDIVVAATSCTYEAFQEACEIEVF 544
Query: 177 DPLKFPATTAWMTNFLSHPVIKDTLPPRDKMI 208
P K P W+ N HP++K+TLPP +K++
Sbjct: 545 TPEKMPKFFIWVNNLKEHPLVKETLPPHEKLV 640
>TC77968 similar to GP|11385437|gb|AAG34801.1 glutathione S-transferase GST
11 {Glycine max}, partial (96%)
Length = 1009
Score = 181 bits (460), Expect = 2e-46
Identities = 94/217 (43%), Positives = 141/217 (64%), Gaps = 1/217 (0%)
Frame = +2
Query: 4 QDVKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHK 63
++V+LL SPF RV+ ALKLKG+E++YVEE + N S LL+ NPV++ +PVLVH
Sbjct: 110 EEVQLLGSVGSPFVIRVQIALKLKGIEHKYVEEKLGNLSETLLKYNPVYRMVPVLVHNGN 289
Query: 64 TIAESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGD-EQE 122
I+ES +IIEYIDE WKQ P+LP DPY+RAL RFW+ F ++K + ++ + + E+E
Sbjct: 290 PISESRVIIEYIDEAWKQNPILPSDPYKRALDRFWSKFIDEKCTIAAWKSVFMPDEKERE 469
Query: 123 KALNEAREAMEKIEEVIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFP 182
KA E EA++ +E ++ K FFGGE IG++DIA + +I +++EV + KFP
Sbjct: 470 KAREELFEALQFLENELKDK-FFGGEEIGFVDIA----ALFIPLFQEVAEKQLFPGDKFP 634
Query: 183 ATTAWMTNFLSHPVIKDTLPPRDKMIDYFHGRKKDLS 219
W +F +HPV+K+ +P +++ YF R L+
Sbjct: 635 KLQKWSLDFYNHPVVKEVMPSKEQQFGYFKARAASLA 745
>TC85908 similar to GP|11385445|gb|AAG34805.1 glutathione S-transferase GST
15 {Glycine max}, partial (65%)
Length = 970
Score = 177 bits (450), Expect = 2e-45
Identities = 89/207 (42%), Positives = 127/207 (60%), Gaps = 2/207 (0%)
Frame = +2
Query: 6 VKLLSFWPSPFSKRVEWALKLKGVEYEYVEEDIFNKSSLLLELNPVHKKIPVLVHGHKTI 65
VKL FW SPF+ RV W L LKG+ YE +EE FNKS LLL+ NP++KK PVL+H K I
Sbjct: 167 VKLHGFWYSPFTLRVVWTLNLKGIPYENIEEYRFNKSPLLLQYNPIYKKTPVLIHDGKPI 346
Query: 66 AESFIIIEYIDETWKQYPLLPHDPYERALARFWANFTEQKLLETVFVAMCITGDEQEKAL 125
ES II+EYIDE W LLP DPY+RA ARFW + ++ + I+G+E+EKA
Sbjct: 347 CESMIIVEYIDEIWPHNSLLPADPYDRAQARFWVKYVDELISAIEPFLNSISGEEREKAR 526
Query: 126 NEAREAMEKIEE--VIEGKRFFGGENIGYLDIAVGWISYWIHIWEEVGSIHIIDPLKFPA 183
E ++ IE+ + + K+ +GG+ I +DIA+G +I I E++ + ++ +FP
Sbjct: 527 ENLWERLKVIEDWCLDDKKKLYGGDTINIVDIALGSFVKFIEIQEDMFEVKVLQSERFPR 706
Query: 184 TTAWMTNFLSHPVIKDTLPPRDKMIDY 210
W NF PVIK P ++K++ +
Sbjct: 707 LHLWFNNFKDVPVIKGNTPGQEKLVAF 787
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.140 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,170,969
Number of Sequences: 36976
Number of extensions: 122320
Number of successful extensions: 659
Number of sequences better than 10.0: 94
Number of HSP's better than 10.0 without gapping: 613
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 617
length of query: 228
length of database: 9,014,727
effective HSP length: 93
effective length of query: 135
effective length of database: 5,575,959
effective search space: 752754465
effective search space used: 752754465
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0162b.4