BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0153b.6
(156 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86100 similar to GP|20521311|dbj|BAB91825. hypothetical protei... 254 1e-68
TC88825 weakly similar to GP|3193298|gb|AAC19282.1| T14P8.17 gen... 84 2e-17
BM815833 weakly similar to GP|15810603|gb unknown protein {Arabi... 75 8e-15
TC82684 weakly similar to GP|14423480|gb|AAK62422.1 Unknown prot... 75 8e-15
TC77770 weakly similar to GP|18419639|gb|AAL69386.1 unknown {Nar... 61 2e-10
TC77634 weakly similar to GP|22136054|gb|AAM91609.1 unknown prot... 50 5e-07
BE240622 similar to GP|10177986|db gb|AAF07835.1~gene_id:MJG14.7... 49 8e-07
BF649108 similar to GP|21592465|gb| unknown {Arabidopsis thalian... 33 2e-05
TC85562 similar to GP|6403495|gb|AAF07835.1| unknown protein {Ar... 44 2e-05
TC92680 weakly similar to GP|9758945|dbj|BAB09332.1 gb|AAF02153.... 37 0.002
BE999754 similar to GP|4335763|gb| expressed protein {Arabidopsi... 36 0.007
BE248698 weakly similar to GP|12836661|db H6 homeo box 3~data so... 29 0.68
TC78493 weakly similar to GP|15010698|gb|AAK74008.1 AT3g22600/F1... 28 2.0
TC80462 similar to PIR|S18973|S18973 glutathione reductase (NADP... 27 2.6
TC89433 similar to GP|13277220|emb|CAC34409. ZF-HD homeobox prot... 27 2.6
TC89583 similar to GP|2108311|emb|CAB08855.1 IgG1/kappa antibody... 27 2.6
TC78080 similar to GP|16904226|gb|AAL30820.1 calcium/calmodulin-... 27 3.4
BE322935 similar to GP|18073605|emb hypothetical protein {Grapev... 27 3.4
BQ149793 27 3.4
TC77478 similar to GP|9884651|dbj|BAB11979.1 MGDG synthase type ... 27 4.4
>TC86100 similar to GP|20521311|dbj|BAB91825. hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (23%)
Length = 955
Score = 254 bits (649), Expect = 1e-68
Identities = 121/155 (78%), Positives = 136/155 (87%)
Frame = +1
Query: 1 MNPKILILFALFLNLTSKSFQNPNPKPPKSTPSSAHIELTNYGFPVGLLPDTTVLGFAVN 60
MNPKILI+F L LNLTSKS QNPNPK PK PS AHIEL YGFP+GLLP TTVL +N
Sbjct: 31 MNPKILIVFILLLNLTSKSIQNPNPKTPKPIPSPAHIELATYGFPIGLLPATTVLNHFIN 210
Query: 61 QTSGDFSVRLGGACKITLPPDNYVATYSNTITGKIVKGRIAELNGIRVRAFFQWWSITGI 120
+TSG+FSV+L GACKITLPPDNYVATYS+TITGKIVKG+IAEL+GIRVRAFF+WW ITGI
Sbjct: 211 KTSGEFSVKLAGACKITLPPDNYVATYSDTITGKIVKGKIAELDGIRVRAFFKWWGITGI 390
Query: 121 RSSGDNIVFEVGMVTAKYPSKNFDDSPACEGQRSS 155
RSSGD++VFEVG+VTAKYP KNF DSP CEG+ S+
Sbjct: 391 RSSGDDVVFEVGVVTAKYPVKNFVDSPPCEGKHSA 495
>TC88825 weakly similar to GP|3193298|gb|AAC19282.1| T14P8.17 gene product
{Arabidopsis thaliana}, partial (79%)
Length = 638
Score = 84.0 bits (206), Expect = 2e-17
Identities = 51/155 (32%), Positives = 87/155 (55%), Gaps = 7/155 (4%)
Frame = +3
Query: 2 NPKILILFAL------FLNLT-SKSFQNPNPKPPKSTPSSAHIELTNYGFPVGLLPDTTV 54
N K++I+++L ++LT S S + PK+ SA+ L YGFP+GLLP +
Sbjct: 141 NSKLVIMYSLKSVLILLISLTISSSLSSCTLIEPKNL--SAYDLLMEYGFPMGLLPKGAI 314
Query: 55 LGFAVNQTSGDFSVRLGGACKITLPPDNYVATYSNTITGKIVKGRIAELNGIRVRAFFQW 114
G+++N+ +G FSV C + ++Y +Y +TI+G I + R+ +L G+ VR W
Sbjct: 315 -GYSLNRETGQFSVYFEKTCSFVI--ESYTLSYKSTISGVISQNRLYKLKGVSVRILLLW 485
Query: 115 WSITGIRSSGDNIVFEVGMVTAKYPSKNFDDSPAC 149
+I + G++I F VG+ +A + +NF + P C
Sbjct: 486 LNIVEVSRKGNDIDFSVGITSAGFGVENFLECPQC 590
>BM815833 weakly similar to GP|15810603|gb unknown protein {Arabidopsis
thaliana}, partial (64%)
Length = 778
Score = 75.5 bits (184), Expect = 8e-15
Identities = 45/145 (31%), Positives = 70/145 (48%)
Frame = +3
Query: 5 ILILFALFLNLTSKSFQNPNPKPPKSTPSSAHIELTNYGFPVGLLPDTTVLGFAVNQTSG 64
I + ++L L+ QN N + S + L Y FPVG+LP G+ +N+ +G
Sbjct: 153 IFLSILIYLTLSHALIQNEN------SSESVYEILKQYDFPVGILPQGAT-GYELNKNTG 311
Query: 65 DFSVRLGGACKITLPPDNYVATYSNTITGKIVKGRIAELNGIRVRAFFQWWSITGIRSSG 124
F+V C P +Y Y +TI G I KG++++L G+ V+ W I +
Sbjct: 312 KFTVYFDDTC--IFPIKSYDLKYKSTIKGVISKGKLSKLKGVTVKVELLWLKIVEVTRHD 485
Query: 125 DNIVFEVGMVTAKYPSKNFDDSPAC 149
D + F VG+ AK+ NF + P C
Sbjct: 486 DYLQFSVGIAYAKFGIDNFLEIPQC 560
>TC82684 weakly similar to GP|14423480|gb|AAK62422.1 Unknown protein
{Arabidopsis thaliana}, partial (65%)
Length = 683
Score = 75.5 bits (184), Expect = 8e-15
Identities = 39/110 (35%), Positives = 64/110 (57%)
Frame = +1
Query: 34 SAHIELTNYGFPVGLLPDTTVLGFAVNQTSGDFSVRLGGACKITLPPDNYVATYSNTITG 93
+A+ L Y FP G+LP V + ++++SG+F L +C +L +Y +Y +TITG
Sbjct: 169 TAYEALRTYNFPPGILP-IGVTNYELDKSSGNFRADLNRSCSFSLQ-GSYQLSYKSTITG 342
Query: 94 KIVKGRIAELNGIRVRAFFQWWSITGIRSSGDNIVFEVGMVTAKYPSKNF 143
KI + R+ +L GI V+ F W +I + DN+ F VG+ +A +P +F
Sbjct: 343 KITENRLTDLRGISVKVMFFWVNILEVVRKSDNLDFSVGVASAAFPLDDF 492
>TC77770 weakly similar to GP|18419639|gb|AAL69386.1 unknown {Narcissus
pseudonarcissus}, partial (75%)
Length = 916
Score = 60.8 bits (146), Expect = 2e-10
Identities = 38/114 (33%), Positives = 61/114 (53%), Gaps = 3/114 (2%)
Frame = +1
Query: 39 LTNYGFPVGLLPDTTVLGFAVNQTSGDFSVRLGGACKITLPPDNYVATYSNTITGKIVKG 98
L +G P GLLP++ V ++++ G F V L C I +Y+ Y TITGK+ G
Sbjct: 172 LPKFGLPSGLLPNS-VTDYSLSD-DGRFVVHLSDTCYIQF---DYLVYYEKTITGKLSYG 336
Query: 99 RIAELNGIRVRAFFQWWSITGIR---SSGDNIVFEVGMVTAKYPSKNFDDSPAC 149
I++L GI+V+ F W+++ IR ++I F+VG++ K F +C
Sbjct: 337 SISDLKGIQVQRVFIWFNVDEIRVDLPPSNSIYFQVGIINKKLNVDQFKTVHSC 498
>TC77634 weakly similar to GP|22136054|gb|AAM91609.1 unknown protein
{Arabidopsis thaliana}, partial (53%)
Length = 1134
Score = 49.7 bits (117), Expect = 5e-07
Identities = 36/128 (28%), Positives = 57/128 (44%), Gaps = 3/128 (2%)
Frame = +2
Query: 30 STPSSAHIELTNYGFPVGLLPDTTVLGFAVNQTSGDFSVRLGGACKITLPPDNYVATYSN 89
S +S + L N+G P+G+ P V F V + G F V L AC + + Y
Sbjct: 242 SNATSIYEVLFNHGLPMGIFP-KGVNEFNVGE-DGKFWVHLDQACNAKFENELH---YDR 406
Query: 90 TITGKIVKGRIAELNGIRVRAFFQWWSITGIR---SSGDNIVFEVGMVTAKYPSKNFDDS 146
++G + G+I L G+ + F W+ + IR S I F+VG ++ F+
Sbjct: 407 NVSGSLSYGKIDALTGLEAQDLFLWFPVMSIRVDVPSSGLIYFDVGAAYKQFSLSLFETP 586
Query: 147 PACEGQRS 154
P C R+
Sbjct: 587 PECVAVRT 610
>BE240622 similar to GP|10177986|db gb|AAF07835.1~gene_id:MJG14.7~similar to
unknown protein {Arabidopsis thaliana}, partial (94%)
Length = 544
Score = 48.9 bits (115), Expect = 8e-07
Identities = 27/96 (28%), Positives = 49/96 (50%), Gaps = 1/96 (1%)
Frame = +3
Query: 39 LTNYGFPVGLLP-DTTVLGFAVNQTSGDFSVRLGGACKITLPPDNYVATYSNTITGKIVK 97
L Y P+G+ P D T + N+ +G V + C+++ D+ V + T+TG + K
Sbjct: 147 LKEYDLPIGIFPRDAT--NYEFNEETGKLEVFIPQVCEVSYK-DSSVLRFFTTVTGYLEK 317
Query: 98 GRIAELNGIRVRAFFQWWSITGIRSSGDNIVFEVGM 133
G++A++ G++ + W +T I S G + GM
Sbjct: 318 GKLADIEGMKTKVII-WVKVTTIFSEGSKLYVTAGM 422
>BF649108 similar to GP|21592465|gb| unknown {Arabidopsis thaliana}, partial
(33%)
Length = 504
Score = 32.7 bits (73), Expect(2) = 2e-05
Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Frame = +3
Query: 98 GRIAELNGIRVRAFFQWWSITGIR---SSGDNIVFEVGMVTAKYPSKNFDDSPAC 149
G+I L+G+ + F W+ + GIR S I F+VG+ ++ +D P C
Sbjct: 300 GKIKGLSGMTAQELFLWFPVKGIRVDLPSSGLIHFDVGVADKQFSLSLXEDPPDC 464
Score = 31.2 bits (69), Expect(2) = 2e-05
Identities = 17/45 (37%), Positives = 25/45 (54%)
Frame = +2
Query: 30 STPSSAHIELTNYGFPVGLLPDTTVLGFAVNQTSGDFSVRLGGAC 74
S ++ + EL G PVGLLP + + +N TSG+F V + C
Sbjct: 107 SNSTTIYDELRAKGLPVGLLP-KGITRYEINGTSGEFQVWMKEPC 238
>TC85562 similar to GP|6403495|gb|AAF07835.1| unknown protein {Arabidopsis
thaliana}, partial (63%)
Length = 720
Score = 44.3 bits (103), Expect = 2e-05
Identities = 25/90 (27%), Positives = 46/90 (50%), Gaps = 1/90 (1%)
Frame = +2
Query: 45 PVGLLP-DTTVLGFAVNQTSGDFSVRLGGACKITLPPDNYVATYSNTITGKIVKGRIAEL 103
P+G+ P D T + N+ +G V + C++ D+ V + T+TG + KG++A++
Sbjct: 248 PIGIFPRDAT--NYEFNEETGKLEVFIPQVCEVGYK-DSSVLRFFTTVTGYLEKGKLADI 418
Query: 104 NGIRVRAFFQWWSITGIRSSGDNIVFEVGM 133
G++ + W +T I S G + GM
Sbjct: 419 EGMKTKVII-WVKVTTIFSEGSKLYVTAGM 505
>TC92680 weakly similar to GP|9758945|dbj|BAB09332.1
gb|AAF02153.1~gene_id:MRB17.3~similar to unknown protein
{Arabidopsis thaliana}, partial (74%)
Length = 708
Score = 37.4 bits (85), Expect = 0.002
Identities = 30/123 (24%), Positives = 51/123 (41%), Gaps = 3/123 (2%)
Frame = +1
Query: 33 SSAHIELTNYGFPVGLLPDTTVLGFAVNQTSGDFSVRLGGACKITLPPDNYVATYSNTIT 92
SS H L + G P GLLP+ + + ++ +G V L C Y + IT
Sbjct: 85 SSIHELLRSKGLPAGLLPE-EIKSYTFSE-NGHLEVFLESPCLTKYENRVY---FEQVIT 249
Query: 93 GKIVKGRIAELNGIRVRAFFQWWSITGI---RSSGDNIVFEVGMVTAKYPSKNFDDSPAC 149
+ G + + G++ F W + I S I+F++G+ + F+ P C
Sbjct: 250 ANLTYGSLIGVEGLQQEELFVWLPVKDIIVDDPSSGLILFDIGLAYKQLSFSLFEVPPHC 429
Query: 150 EGQ 152
+ Q
Sbjct: 430 KPQ 438
>BE999754 similar to GP|4335763|gb| expressed protein {Arabidopsis thaliana},
partial (78%)
Length = 593
Score = 35.8 bits (81), Expect = 0.007
Identities = 22/95 (23%), Positives = 43/95 (45%)
Frame = +1
Query: 26 KPPKSTPSSAHIELTNYGFPVGLLPDTTVLGFAVNQTSGDFSVRLGGACKITLPPDNYVA 85
K KS P L + P GL P + + +++ G V L ++ D+ +
Sbjct: 121 KTQKSLPDL----LREHNLPPGLFP-RNITCYEFDESKGKLMVHLSSPSEVCFK-DSSIV 282
Query: 86 TYSNTITGKIVKGRIAELNGIRVRAFFQWWSITGI 120
YSN + G + KG++ ++G++ + W +T +
Sbjct: 283 RYSNRVKGTLSKGKLIVIDGMKTKVLV-WVKVTSV 384
>BE248698 weakly similar to GP|12836661|db H6 homeo box 3~data source:MGD
source key:MGI:107160 evidence:ISS~putative {Mus
musculus}, partial (3%)
Length = 416
Score = 29.3 bits (64), Expect = 0.68
Identities = 14/30 (46%), Positives = 20/30 (66%)
Frame = +2
Query: 94 KIVKGRIAELNGIRVRAFFQWWSITGIRSS 123
K+V+G EL+ VR FF+ SITG+ +S
Sbjct: 278 KVVEGLKEELDEFSVRMFFKGLSITGVENS 367
>TC78493 weakly similar to GP|15010698|gb|AAK74008.1 AT3g22600/F16J14_17
{Arabidopsis thaliana}, partial (57%)
Length = 797
Score = 27.7 bits (60), Expect = 2.0
Identities = 13/32 (40%), Positives = 19/32 (58%)
Frame = +2
Query: 55 LGFAVNQTSGDFSVRLGGACKITLPPDNYVAT 86
LG ++NQT ++ L GACK+ PP + T
Sbjct: 305 LGISINQTQ---ALALPGACKVQTPPTSQCKT 391
>TC80462 similar to PIR|S18973|S18973 glutathione reductase (NADPH) (EC
1.6.4.2) - garden pea, partial (31%)
Length = 669
Score = 27.3 bits (59), Expect = 2.6
Identities = 14/32 (43%), Positives = 20/32 (61%)
Frame = +2
Query: 6 LILFALFLNLTSKSFQNPNPKPPKSTPSSAHI 37
L++F+L +LTS QNPN S PS+ H+
Sbjct: 104 LLIFSLLQSLTSSLHQNPN----LSIPSTPHL 187
>TC89433 similar to GP|13277220|emb|CAC34409. ZF-HD homeobox protein
{Flaveria bidentis}, partial (52%)
Length = 1430
Score = 27.3 bits (59), Expect = 2.6
Identities = 14/32 (43%), Positives = 19/32 (58%)
Frame = +1
Query: 17 SKSFQNPNPKPPKSTPSSAHIELTNYGFPVGL 48
S+S +P+P P S PS+ H+ L G VGL
Sbjct: 739 SRSPSSPSPPPISSYPSAPHMLLALSGAGVGL 834
>TC89583 similar to GP|2108311|emb|CAB08855.1 IgG1/kappa antibody {Mus
musculus}, partial (8%)
Length = 756
Score = 27.3 bits (59), Expect = 2.6
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -1
Query: 116 SITGIRSSGDNIVFEVGMVTAKYPSKNFDDSPACEGQRSSS 156
++TGI SG +V + +V P+ F +SP + SSS
Sbjct: 621 TVTGIIDSGMEVVLLIFLVFGSNPNLPFIESPRLSSKSSSS 499
>TC78080 similar to GP|16904226|gb|AAL30820.1 calcium/calmodulin-dependent
protein kinase CaMK3 {Nicotiana tabacum}, partial (46%)
Length = 1279
Score = 26.9 bits (58), Expect = 3.4
Identities = 13/26 (50%), Positives = 17/26 (65%), Gaps = 3/26 (11%)
Frame = -1
Query: 14 NLTSKSFQNPN---PKPPKSTPSSAH 36
NL +KSF+NPN P S+PSS +
Sbjct: 265 NLLAKSFENPNLLSKSPAPSSPSSGY 188
>BE322935 similar to GP|18073605|emb hypothetical protein {Grapevine fleck
virus}, partial (21%)
Length = 698
Score = 26.9 bits (58), Expect = 3.4
Identities = 19/55 (34%), Positives = 24/55 (43%)
Frame = +2
Query: 12 FLNLTSKSFQNPNPKPPKSTPSSAHIELTNYGFPVGLLPDTTVLGFAVNQTSGDF 66
F N +S S P P PP S PS+ L + P L T + F +N S F
Sbjct: 236 FTNPSSLSSPPPEPSPPPSPPSTVSPSLNIFSPPNVFL--TGLAPFRINTISPIF 394
>BQ149793
Length = 534
Score = 26.9 bits (58), Expect = 3.4
Identities = 14/38 (36%), Positives = 23/38 (59%)
Frame = -3
Query: 5 ILILFALFLNLTSKSFQNPNPKPPKSTPSSAHIELTNY 42
+L+ + L LT ++FQ+P PK TP+ ++EL Y
Sbjct: 427 VLLSKKVVLVLTIRTFQSP----PKPTPAVVYLELFKY 326
>TC77478 similar to GP|9884651|dbj|BAB11979.1 MGDG synthase type A {Glycine
max}, partial (85%)
Length = 2229
Score = 26.6 bits (57), Expect = 4.4
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = -2
Query: 19 SFQNPNPKPPKSTPSSAHIELTNYGFPVGLLPDTTV 54
SF +P P PP +P S + N P+G+L T+
Sbjct: 797 SFPSPTPPPPPPSPPSPTLNEAN---PIGILSQ*TL 699
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.136 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,920,168
Number of Sequences: 36976
Number of extensions: 68099
Number of successful extensions: 579
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 560
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 574
length of query: 156
length of database: 9,014,727
effective HSP length: 88
effective length of query: 68
effective length of database: 5,760,839
effective search space: 391737052
effective search space used: 391737052
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0153b.6