BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0150.6
(396 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80444 similar to GP|15450515|gb|AAK96550.1 At2g17400 {Arabidop... 363 e-101
BG585603 similar to PIR|A96793|A967 probable DNA-binding protein... 107 6e-24
BF004903 similar to PIR|B96789|B96 protein T23E18.4 [imported] -... 49 3e-06
TC81814 weakly similar to GP|9294541|dbj|BAB02804.1 high mobilit... 45 6e-05
TC92211 similar to PIR|B86182|B86182 hypothetical protein [impor... 44 1e-04
TC83932 similar to GP|15294292|gb|AAK95323.1 At2g25920/F17H15.5 ... 34 0.11
TC89603 similar to PIR|S71566|S71566 heat shock protein 17.7K -... 33 0.19
TC77395 similar to GP|4874305|gb|AAD31367.1| expressed protein {... 31 0.73
TC80066 similar to PIR|T50518|T50518 ABC transporter-like protei... 31 0.73
TC92903 weakly similar to GP|6137153|gb|AAF04361.1| low molecula... 31 0.73
TC87543 similar to PIR|A96745|A96745 probable cytosolic factor T... 31 0.95
TC82415 homologue to GP|9759314|dbj|BAB09681.1 splicing factor 3... 30 1.2
CB894389 similar to SP|P27879|HS11_ 18.1 kDa class I heat shock ... 30 1.6
TC79995 similar to GP|10178093|dbj|BAB11480. gene_id:MVP2.2~pir|... 30 2.1
TC80099 similar to GP|21553676|gb|AAM62769.1 50S ribosomal prote... 29 2.8
TC88992 similar to SP|P19243|HS11_PEA 18.1 kDa class I heat shoc... 28 4.7
AW586945 homologue to GP|23496297|gb hypothetical protein {Plasm... 28 6.2
TC77261 homologue to GP|8777330|dbj|BAA96920.1 26S proteasome AA... 28 6.2
TC85568 homologue to SP|P27880|HS12_MEDSA 18.2 kDa class I heat ... 28 6.2
TC81511 similar to GP|20259627|gb|AAM14170.1 unknown protein {Ar... 28 6.2
>TC80444 similar to GP|15450515|gb|AAK96550.1 At2g17400 {Arabidopsis
thaliana}, partial (31%)
Length = 1118
Score = 363 bits (933), Expect = e-101
Identities = 183/282 (64%), Positives = 213/282 (74%), Gaps = 29/282 (10%)
Frame = +1
Query: 141 ERSLEFKPPKFYGEPLNCLKLWRAVIRLGGYDVVTGSKLWRQVGESFHPPKTCTTVSWTF 200
ERS++FK PKFYGE LNCLKLWRAV+RLGGY+ VT KLWR VGESF PPKTCTTVSWTF
Sbjct: 19 ERSMDFKHPKFYGEWLNCLKLWRAVMRLGGYEKVTSCKLWRSVGESFKPPKTCTTVSWTF 198
Query: 201 RIFYEKALLEYEKHKREIGELQLPVGVFPQPSSVEKETTVYQAPGSGRARRDAAARAMQG 260
R FYEKALL+YE+H + GEL +P+ P+P +VE Q SGR+RR AA +AM+G
Sbjct: 199 RGFYEKALLDYERHNIKGGELNVPIASPPEPVNVEN-----QGLASGRSRRYAAEQAMRG 363
Query: 261 WHAQRLLGYGEVAEPVIKDKNFNPTTKREKNLKSI------------------------- 295
WH+QRLLG GEV +P+IKD+N KREK LKSI
Sbjct: 364 WHSQRLLGNGEVGDPIIKDRNAVSVQKREKQLKSINVPKRKSPSYMDNAVKAARSKPSRP 543
Query: 296 ----AVVDVGPPADWVKINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITGMPEHI 351
AVVD+GP ADWVK+NV++TKDCFE+YALVPGLLREEVRVQSDP GRLVI+G PEH
Sbjct: 544 QLGTAVVDIGPSADWVKVNVQKTKDCFEIYALVPGLLREEVRVQSDPAGRLVISGEPEHP 723
Query: 352 DNPWGITPFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFEQ 393
+NPWG+TPFKKVV+LP+RIDP QTSAVV+LHG+LFVRVPFEQ
Sbjct: 724 NNPWGVTPFKKVVSLPSRIDPHQTSAVVTLHGQLFVRVPFEQ 849
>BG585603 similar to PIR|A96793|A967 probable DNA-binding protein
48797-45229 [imported] - Arabidopsis thaliana, partial
(10%)
Length = 256
Score = 107 bits (268), Expect = 6e-24
Identities = 51/53 (96%), Positives = 52/53 (97%)
Frame = -1
Query: 344 ITGMPEHIDNPWGITPFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFEQGAV 396
ITGMPE+ DNPWGITPFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFEQGAV
Sbjct: 256 ITGMPENADNPWGITPFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFEQGAV 98
>BF004903 similar to PIR|B96789|B96 protein T23E18.4 [imported] - Arabidopsis
thaliana, partial (30%)
Length = 709
Score = 49.3 bits (116), Expect = 3e-06
Identities = 34/107 (31%), Positives = 46/107 (42%), Gaps = 5/107 (4%)
Frame = +2
Query: 112 NDTEVVDYDEPGASL-----EREAFMKELENFYRERSLEFKPPKFYGEPLNCLKLWRAVI 166
ND E Y P AS + F L F+ + +F P G+ L+ L+ V
Sbjct: 383 NDDEAKHYPPPLASHNDVVNDPTLFWDTLRRFHFLMATKFMIPVIGGKELDLHVLYVEVT 562
Query: 167 RLGGYDVVTGSKLWRQVGESFHPPKTCTTVSWTFRIFYEKALLEYEK 213
R GY+ V K WR+VG F T T+ S+ R Y L YE+
Sbjct: 563 RRSGYEKVVAEKKWREVGSVFRFSSTTTSASFVLRKHYLNLLYHYEQ 703
>TC81814 weakly similar to GP|9294541|dbj|BAB02804.1 high mobility group
protein-like {Arabidopsis thaliana}, partial (33%)
Length = 867
Score = 44.7 bits (104), Expect = 6e-05
Identities = 33/131 (25%), Positives = 62/131 (47%), Gaps = 2/131 (1%)
Frame = +1
Query: 85 DVKTPLHQELVTPKSRERNVREMKSVLNDTEVVDYDEPGASLEREA--FMKELENFYRER 142
++K ++Q ++ +N E +S N P + L R++ F ++L++F+
Sbjct: 88 EIKMSINQNQNQNQNPNQNQCESESKSNTNPYPPPTAPYSDLVRDSNLFQQKLQSFHDSL 267
Query: 143 SLEFKPPKFYGEPLNCLKLWRAVIRLGGYDVVTGSKLWRQVGESFHPPKTCTTVSWTFRI 202
+ K P G+PL+ L+ V GG + V + W++V SF+ T T+ S+ R
Sbjct: 268 GTKLKIPTIGGKPLDLHHLFVEVTSRGGIEKVIVDRKWKEVIMSFNFRDTITSGSFMVRK 447
Query: 203 FYEKALLEYEK 213
Y L +E+
Sbjct: 448 TYLSLLYHFEQ 480
>TC92211 similar to PIR|B86182|B86182 hypothetical protein [imported] -
Arabidopsis thaliana, partial (31%)
Length = 798
Score = 43.9 bits (102), Expect = 1e-04
Identities = 27/101 (26%), Positives = 46/101 (44%), Gaps = 5/101 (4%)
Frame = +3
Query: 118 DYDEPGASLER-----EAFMKELENFYRERSLEFKPPKFYGEPLNCLKLWRAVIRLGGYD 172
+Y P A+ E + F+ LE + +F P G L+ +L+ V GG++
Sbjct: 123 EYPPPMATYEEVVDNPKLFILCLEKLHTLMGTKFMIPVIGGRELDLHRLFVEVTSRGGFE 302
Query: 173 VVTGSKLWRQVGESFHPPKTCTTVSWTFRIFYEKALLEYEK 213
+ + W++V F+ P T T S+ R +Y L YE+
Sbjct: 303 KIIKDRKWKEVTLVFNFPSTATNASFVLRKYYTSLLYHYEQ 425
>TC83932 similar to GP|15294292|gb|AAK95323.1 At2g25920/F17H15.5
{Arabidopsis thaliana}, partial (45%)
Length = 871
Score = 33.9 bits (76), Expect = 0.11
Identities = 23/73 (31%), Positives = 33/73 (44%), Gaps = 15/73 (20%)
Frame = +2
Query: 28 DQNAGSSPMVLAPNSL-AQIPKDEVMVDQGDDDNR--------------IGDSTPNNMLE 72
D N G SP +P+S + P++EV VD R +GDS P+N++E
Sbjct: 107 DSNLGGSPSSSSPSSKRTRDPEEEVYVDNLRSHKRYLSEIMASSLNGLTVGDSLPDNLME 286
Query: 73 VKTGSENQLELED 85
SE+ L D
Sbjct: 287 SPARSESAFSLRD 325
>TC89603 similar to PIR|S71566|S71566 heat shock protein 17.7K - common
sunflower, partial (64%)
Length = 1320
Score = 33.1 bits (74), Expect = 0.19
Identities = 31/104 (29%), Positives = 47/104 (44%), Gaps = 14/104 (13%)
Frame = +1
Query: 303 PADWVKINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITGMPEH-------IDNPW 355
P DW +ETKD + +PGL +E+V V+ D L I+G H DN W
Sbjct: 85 PTDW-----KETKDAHVFISDLPGLKKEDVNVEIDEGKVLQISGERTHNVDENDEKDNKW 249
Query: 356 GIT-----PFKKVVNLP--ARIDPLQTSAVVSLHGRLFVRVPFE 392
F++ LP A++D ++ + +G L V +P E
Sbjct: 250 HHVERCRGKFQRRFRLPQNAKVDQVKANME---NGVLIVTIPKE 372
>TC77395 similar to GP|4874305|gb|AAD31367.1| expressed protein {Arabidopsis
thaliana}, partial (65%)
Length = 1649
Score = 31.2 bits (69), Expect = 0.73
Identities = 29/125 (23%), Positives = 50/125 (39%), Gaps = 8/125 (6%)
Frame = +2
Query: 14 VDDD------TTLLPQNSDKDQNAGSSPMVLAPNSLAQIPKDEVMVDQGDDDNRIGDSTP 67
VDDD TT PQ SD P P +E ++D GD++ D P
Sbjct: 512 VDDDDDDYYLTTAPPQFSDPQ------PSEDKPEHFEDTESEEDILDDGDEEEEEQDHEP 673
Query: 68 NNMLEVKTGSE--NQLELEDVKTPLHQELVTPKSRERNVREMKSVLNDTEVVDYDEPGAS 125
VKT E E+ + ++L + +++ + E++++L D V +
Sbjct: 674 EPEHAVKTEPEVKKHAEVPVMPKEAERQLSKKERKKKELEELEALLADFGVTQKESNDGQ 853
Query: 126 LEREA 130
+ E+
Sbjct: 854 SQDES 868
>TC80066 similar to PIR|T50518|T50518 ABC transporter-like protein -
Arabidopsis thaliana, partial (14%)
Length = 992
Score = 31.2 bits (69), Expect = 0.73
Identities = 17/38 (44%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = +3
Query: 110 VLNDTEVVDYDEPGASLERE-AFMKELENFYRERSLEF 146
VL+D + +YDEP LERE +F +L Y RS F
Sbjct: 345 VLSDGRIAEYDEPSKLLEREDSFFYKLIKEYSSRSHSF 458
>TC92903 weakly similar to GP|6137153|gb|AAF04361.1| low molecular weight
heat stress protein {Streptococcus thermophilus},
partial (25%)
Length = 503
Score = 31.2 bits (69), Expect = 0.73
Identities = 24/78 (30%), Positives = 38/78 (47%), Gaps = 9/78 (11%)
Frame = +2
Query: 309 INVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITGMPEHIDNPWGIT---------P 359
++V ETKD F V A +PG+ ++++ V LVI+G + +N G +
Sbjct: 215 LDVHETKDEFVVNAELPGIKKDKISVDIKD-NNLVISGENKRDENFKGASVHIQERQYGN 391
Query: 360 FKKVVNLPARIDPLQTSA 377
F + V+LP I SA
Sbjct: 392 FSRTVSLPPNIKTTNISA 445
>TC87543 similar to PIR|A96745|A96745 probable cytosolic factor T9N14.8
[imported] - Arabidopsis thaliana, partial (74%)
Length = 1718
Score = 30.8 bits (68), Expect = 0.95
Identities = 29/111 (26%), Positives = 43/111 (38%), Gaps = 14/111 (12%)
Frame = +1
Query: 269 YGEVAEPVIKDKNFNPTTKREKNL--------KSIAVVDVGPPADWVKINVRETKDCFEV 320
YGE + +K F KRE+ L KSI +D P V + K+
Sbjct: 703 YGEFQNKELYEKTFGSEEKRERFLRWRVQFLEKSIRKLDFSPGGVNTLFQVNDLKNS--- 873
Query: 321 YALVPGLLREEVRVQSDPVGRLVITGMPE------HIDNPWGITPFKKVVN 365
PG ++E+RV + L+ PE I+ PW F ++N
Sbjct: 874 ----PGPAKKELRVATKMALELLQDNYPEFVAKQVFINVPWWYLAFYTILN 1014
>TC82415 homologue to GP|9759314|dbj|BAB09681.1 splicing factor 3a
{Arabidopsis thaliana}, partial (16%)
Length = 654
Score = 30.4 bits (67), Expect = 1.2
Identities = 20/74 (27%), Positives = 36/74 (48%)
Frame = +1
Query: 69 NMLEVKTGSENQLELEDVKTPLHQELVTPKSRERNVREMKSVLNDTEVVDYDEPGASLER 128
N + V GS+ LE + +T E V P+SR R R+ + L ++ + PGA ++R
Sbjct: 88 NEITVN*GSKRALEEDTAET--RSEQVAPRSRGRV*RQRRQHL**EDIY*FTTPGADMKR 261
Query: 129 EAFMKELENFYRER 142
+ + + + R
Sbjct: 262 KNLLSNSSSISKRR 303
>CB894389 similar to SP|P27879|HS11_ 18.1 kDa class I heat shock protein
(Fragment). [Alfalfa] {Medicago sativa}, partial (41%)
Length = 189
Score = 30.0 bits (66), Expect = 1.6
Identities = 16/30 (53%), Positives = 19/30 (63%)
Frame = +1
Query: 308 KINVRETKDCFEVYALVPGLLREEVRVQSD 337
+I+ RET D A +PGL REEVRV D
Sbjct: 67 RIDWRETPDAHVFRADLPGLRREEVRVDFD 156
>TC79995 similar to GP|10178093|dbj|BAB11480.
gene_id:MVP2.2~pir||T01176~similar to unknown protein
{Arabidopsis thaliana}, partial (48%)
Length = 1028
Score = 29.6 bits (65), Expect = 2.1
Identities = 22/84 (26%), Positives = 36/84 (42%)
Frame = +2
Query: 19 TLLPQNSDKDQNAGSSPMVLAPNSLAQIPKDEVMVDQGDDDNRIGDSTPNNMLEVKTGSE 78
+L P+N KDQ S PN+ + D + V DD+N + + + L V E
Sbjct: 191 SLTPKNQVKDQTGSS-----LPNNQHRSLPDPIYVSSSDDENNLSNDDASKQL-VLYDPE 352
Query: 79 NQLELEDVKTPLHQELVTPKSRER 102
++ + TP + P+ R R
Sbjct: 353 TNVDNANQLTPDPIQCRPPRPRSR 424
>TC80099 similar to GP|21553676|gb|AAM62769.1 50S ribosomal protein L4
{Arabidopsis thaliana}, partial (78%)
Length = 1231
Score = 29.3 bits (64), Expect = 2.8
Identities = 25/97 (25%), Positives = 46/97 (46%), Gaps = 7/97 (7%)
Frame = +2
Query: 254 AARAMQGWHAQRLLGYGEVAEPVIKDKNFNPTTKREKNLKSIAVVDVGPPADWVKINVRE 313
+ARA +G +LL + ++ P K KNF + ++ K + +VD GP + +K+ +
Sbjct: 704 SARAAEG----KLLVFEDLEVPTHKTKNFLNFFNQLEDTKKLLIVDDGPVNEKLKLATQN 871
Query: 314 TKDC-------FEVYALVPGLLREEVRVQSDPVGRLV 343
VY++ LL + + + D V R+V
Sbjct: 872 LHYVNLLPSIGLNVYSI---LLHDTLVMSKDAVNRIV 973
>TC88992 similar to SP|P19243|HS11_PEA 18.1 kDa class I heat shock protein
(HSP 18.1). [Garden pea] {Pisum sativum}, partial (94%)
Length = 658
Score = 28.5 bits (62), Expect = 4.7
Identities = 26/95 (27%), Positives = 44/95 (45%), Gaps = 9/95 (9%)
Frame = +1
Query: 308 KINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITG----MPEHIDNPW-----GIT 358
+++ +ET++ +PGL +EEV+V+ + L I+G E D+ W
Sbjct: 169 RVDWKETQEAHVFSVDLPGLKKEEVKVEIEDGNVLQISGERNKEQEEKDDKWHRVERSSG 348
Query: 359 PFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFEQ 393
F + LP + Q A + +G L V VP E+
Sbjct: 349 KFMRRFRLPENVKMDQVKAGME-NGVLTVTVPKEE 450
>AW586945 homologue to GP|23496297|gb hypothetical protein {Plasmodium
falciparum 3D7}, partial (0%)
Length = 677
Score = 28.1 bits (61), Expect = 6.2
Identities = 17/56 (30%), Positives = 31/56 (55%), Gaps = 3/56 (5%)
Frame = +1
Query: 9 DNMEIVDDDTTLLPQNSDKDQNAGSSPMVLAPNSLAQIPKDEVMVDQGD---DDNR 61
+++ + +++ LL QNS + QN+ SSP + K + +VD+ D D+NR
Sbjct: 130 NSLNLNNNELLLLSQNSQQFQNSDSSPFAITAE------KQKTLVDRIDRMQDENR 279
>TC77261 homologue to GP|8777330|dbj|BAA96920.1 26S proteasome AAA-ATPase
subunit RPT3 {Arabidopsis thaliana}, partial (94%)
Length = 1575
Score = 28.1 bits (61), Expect = 6.2
Identities = 28/121 (23%), Positives = 52/121 (42%), Gaps = 11/121 (9%)
Frame = +2
Query: 32 GSSPMVLAPNSLAQIPKDEVMVDQGDDDNRIGDSTPNNMLEVKTGSENQLELEDVKTP-- 89
GSS MVL P L + P D D+ ST +++ + + QLE D++
Sbjct: 95 GSSAMVLDPKPLTEPPPSLSSSSPADTDS---SSTSDDLYTLLKSLQRQLEFIDIQEEYV 265
Query: 90 ------LHQELVTPKSRERNVREMKSVLND-TEVVDYDE--PGASLEREAFMKELENFYR 140
L +EL+ + + ++ + V+ E+VD + G++ +++ L R
Sbjct: 266 KDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDTNNGIVGSTTGSNYYVRILSTINR 445
Query: 141 E 141
E
Sbjct: 446 E 448
>TC85568 homologue to SP|P27880|HS12_MEDSA 18.2 kDa class I heat shock
protein. [Alfalfa] {Medicago sativa}, complete
Length = 982
Score = 28.1 bits (61), Expect = 6.2
Identities = 28/95 (29%), Positives = 43/95 (44%), Gaps = 9/95 (9%)
Frame = +3
Query: 308 KINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITG----MPEHIDNPW-----GIT 358
+I+ +ET + A +PGL +EEV+V+ + L I+G E +N W
Sbjct: 246 RIDWKETPEAHVFKADLPGLKKEEVKVEIEDDRVLQISGERNVEKEDKNNQWHRVERSSG 425
Query: 359 PFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFEQ 393
F + LP Q A + +G L V VP E+
Sbjct: 426 KFMRRFRLPENAKMDQVKAAME-NGVLTVTVPKEE 527
>TC81511 similar to GP|20259627|gb|AAM14170.1 unknown protein {Arabidopsis
thaliana}, partial (41%)
Length = 725
Score = 28.1 bits (61), Expect = 6.2
Identities = 20/67 (29%), Positives = 29/67 (42%), Gaps = 19/67 (28%)
Frame = -1
Query: 199 TFRIFYEKALLEYEKHKREIGEL-------------------QLPVGVFPQPSSVEKETT 239
T+ I YE+ +L ++K K++ E LP+ V P PS EKE T
Sbjct: 665 TYYILYERNVLTHQK*KQKNPEKW*NYWNPVFAHIAFLSLLRSLPLRVLPTPSCTEKEAT 486
Query: 240 VYQAPGS 246
+ A S
Sbjct: 485 LVLAKNS 465
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.135 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,468,057
Number of Sequences: 36976
Number of extensions: 158463
Number of successful extensions: 531
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 528
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 530
length of query: 396
length of database: 9,014,727
effective HSP length: 98
effective length of query: 298
effective length of database: 5,391,079
effective search space: 1606541542
effective search space used: 1606541542
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0150.6