Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0146.2
         (687 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BQ144407 weakly similar to GP|6523547|emb| hydroxyproline-rich g...    31  1.8
BG450209 similar to GP|10177604|db gb|AAD41995.1~gene_id:K21H1.1...    30  3.1
BG581317 homologue to OMNI|NTL01EC003 IS2 hypothetical protein {...    29  5.3
TC76418 similar to GP|16226228|gb|AAL16109.1 At1g20110/T20H2_10 ...    29  5.3
TC89322 similar to GP|15292799|gb|AAK92768.1 unknown protein {Ar...    29  6.9
BQ751369 homologue to GP|4336416|gb|A serum deprivation response...    29  6.9
TC77725 similar to PIR|T07370|T07370 ubiquinol--cytochrome-c red...    29  6.9
BQ139295 similar to GP|2832315|gb|A translation release factor e...    28  9.0

>BQ144407 weakly similar to GP|6523547|emb| hydroxyproline-rich glycoprotein
           DZ-HRGP {Volvox carteri f. nagariensis}, partial (23%)
          Length = 1217

 Score = 30.8 bits (68), Expect = 1.8
 Identities = 21/46 (45%), Positives = 26/46 (55%), Gaps = 2/46 (4%)
 Frame = -3

Query: 374 SSFSPYSKAIRGPFWLR--NSYPNASDSALPKEHHITLWRTLLSPR 417
           SSFSP+S++   P WLR  N +P  S+SA P      L R L  PR
Sbjct: 525 SSFSPHSRSPPRPPWLRPFNIHPLISNSAFPSSSPAEL-RFLPPPR 391


>BG450209 similar to GP|10177604|db gb|AAD41995.1~gene_id:K21H1.13~similar to
           unknown protein {Arabidopsis thaliana}, partial (40%)
          Length = 680

 Score = 30.0 bits (66), Expect = 3.1
 Identities = 10/31 (32%), Positives = 19/31 (61%)
 Frame = -3

Query: 618 PVLPKWVDHININVLANQALPPRKRVTWTKY 648
           P++ + +DH+N+ ++    +PPRK V    Y
Sbjct: 591 PIMIRQMDHVNLRIIEISYVPPRKHVPMDAY 499


>BG581317 homologue to OMNI|NTL01EC003 IS2 hypothetical protein {Escherichia
           coli K12-MG1655}, partial (55%)
          Length = 808

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 26/96 (27%), Positives = 37/96 (38%), Gaps = 2/96 (2%)
 Frame = +2

Query: 23  VPIPEPPNEAEKRAIWKSQVLIPFSVNGTLRAILGPLKVVKHDRPNSLPEEHESFHPSVR 82
           +P P PP        + S    PF+ N    +   P        P+ +P  + SF+P   
Sbjct: 140 IPNPTPPQSDPIPNHYASAP--PFTPNYDYSSTYSPYPP---HNPDHVPSSNPSFNPPPF 304

Query: 83  GEELILAFQPS--YRMPFLSDPKRAFRSAPPNPSAN 116
                L  QPS  Y  P+    +     APPNP+ N
Sbjct: 305 ESSNPLYQQPSQPYYPPYDQQHQTPLNYAPPNPNPN 412


>TC76418 similar to GP|16226228|gb|AAL16109.1 At1g20110/T20H2_10
           {Arabidopsis thaliana}, partial (54%)
          Length = 2020

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 26/96 (27%), Positives = 37/96 (38%), Gaps = 2/96 (2%)
 Frame = +3

Query: 23  VPIPEPPNEAEKRAIWKSQVLIPFSVNGTLRAILGPLKVVKHDRPNSLPEEHESFHPSVR 82
           +P P PP        + S    PF+ N    +   P        P+ +P  + SF+P   
Sbjct: 96  IPNPTPPQSDPIPNHYASAP--PFTPNYDYSSTYSPYPP---HNPDHVPSSNPSFNPPPF 260

Query: 83  GEELILAFQPS--YRMPFLSDPKRAFRSAPPNPSAN 116
                L  QPS  Y  P+    +     APPNP+ N
Sbjct: 261 ESSNPLYQQPSQPYYPPYDQQHQTPLNYAPPNPNPN 368


>TC89322 similar to GP|15292799|gb|AAK92768.1 unknown protein {Arabidopsis
           thaliana}, partial (30%)
          Length = 619

 Score = 28.9 bits (63), Expect = 6.9
 Identities = 21/87 (24%), Positives = 33/87 (37%), Gaps = 3/87 (3%)
 Frame = +1

Query: 327 QDNPLTASNTRIDAHRLETLTPAYDASSFEADFRKYFTMFLELKHYR---SSFSPYSKAI 383
           Q  P    N +    + E LTP  D S++    + Y  + +   HY    +  SP  + I
Sbjct: 151 QSEPEEPQNPQDFMIKPEKLTPTIDTSNWPILLKNYDRLNVRTGHYTPLPAGHSPLKRPI 330

Query: 384 RGPFWLRNSYPNASDSALPKEHHITLW 410
               +L+    N    A P  H +  W
Sbjct: 331 AD--YLKYGVINLDKPANPSSHEVVAW 405


>BQ751369 homologue to GP|4336416|gb|A serum deprivation response {Homo
           sapiens}, partial (2%)
          Length = 485

 Score = 28.9 bits (63), Expect = 6.9
 Identities = 11/48 (22%), Positives = 23/48 (47%)
 Frame = -2

Query: 502 WWSEYWAQISKPLVDCLQCMTDAFLLQKQDPKKTKGMHLAEIRSFQEF 549
           W++ +WA +  PL      +  A ++    P+ T+ +  A   +F +F
Sbjct: 205 WYASFWAMLLSPLSGDTLFVVSALIITSTFPEGTQALASAVFNTFSQF 62


>TC77725 similar to PIR|T07370|T07370 ubiquinol--cytochrome-c reductase (EC
           1.10.2.2) 8.2K chain - potato, complete
          Length = 603

 Score = 28.9 bits (63), Expect = 6.9
 Identities = 18/48 (37%), Positives = 24/48 (49%), Gaps = 1/48 (2%)
 Frame = +1

Query: 294 DIRNTKTVSLNAAGPLWLFQLWLNAVFESLLPV-QDNPLTASNTRIDA 340
           DI  T +  L     LWLFQLW   +F  +L V +DN    +N  + A
Sbjct: 334 DIGETSSCPLVEIFELWLFQLWF*MIFFFVLHVTKDNICPVNNVVMSA 477


>BQ139295 similar to GP|2832315|gb|A translation release factor eRF3
           {Podospora anserina}, partial (9%)
          Length = 668

 Score = 28.5 bits (62), Expect = 9.0
 Identities = 22/62 (35%), Positives = 26/62 (41%)
 Frame = +3

Query: 61  VVKHDRPNSLPEEHESFHPSVRGEELILAFQPSYRMPFLSDPKRAFRSAPPNPSANDKAY 120
           V  H  P S P    S  P  R     LA Q S    FLSDP+R  +S    PS   +  
Sbjct: 468 VAMHQSPRSSPLAQMSLRPRQRR----LAAQRS----FLSDPQRPLKSRXXRPSTRPRPX 623

Query: 121 LK 122
           L+
Sbjct: 624 LE 629


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.321    0.136    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,729,767
Number of Sequences: 36976
Number of extensions: 383366
Number of successful extensions: 1974
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1955
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1974
length of query: 687
length of database: 9,014,727
effective HSP length: 103
effective length of query: 584
effective length of database: 5,206,199
effective search space: 3040420216
effective search space used: 3040420216
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)


Lotus: description of TM0146.2