Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0142.1
         (142 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC93896 similar to GP|21553965|gb|AAM63046.1 unknown {Arabidopsi...    35  0.008
AJ499220 similar to SP|Q09863|YAF Hypothetical protein C29E6.10c...    35  0.013
TC86834 similar to PIR|T46158|T46158 hypothetical protein T4D2.5...    33  0.050
TC77947 homologue to GP|10334491|emb|CAC10207. putative splicing...    32  0.11
AL379805 similar to GP|5821153|dbj RNA binding protein {Homo sap...    31  0.14
BF646001 homologue to GP|20303561|gb| putative kinase {Oryza sat...    30  0.25
AW691010 similar to GP|8656003|gb| Contains similarity to formin...    30  0.25
CA989870 similar to GP|18201934|g kinesin-like protein heavy cha...    30  0.42
TC89483 similar to GP|18377662|gb|AAL66981.1 unknown protein {Ar...    30  0.42
BG456449 EGAD|146422|15 vitellogenin {Anolis pulchellus}, partia...    29  0.55
TC84943 similar to PIR|E86306|E86306 Similar to tuftelin-interac...    29  0.72
TC80239 similar to GP|7208779|emb|CAB76912.1 hypothetical protei...    29  0.72
AJ499968 weakly similar to GP|23495961|gb| hypothetical protein ...    28  0.94
TC85391 homologue to PIR|T06598|T06598 dnaK-type molecular chape...    28  0.94
BE998238 similar to PIR|T39202|T39 heat shock protein 90 homolog...    28  1.2
BQ137028 similar to GP|4914334|gb|A F14N23.20 {Arabidopsis thali...    28  1.2
TC91751 similar to GP|2739382|gb|AAC14505.1| unknown protein {Ar...    28  1.2
TC80245 similar to PIR|T48581|T48581 hypothetical protein T31B5....    28  1.2
TC82291 similar to PIR|D96519|D96519 mysoin-like protein  11013-...    28  1.2
TC85389 homologue to SP|Q03684|BIP4_TOBAC Luminal binding protei...    28  1.2

>TC93896 similar to GP|21553965|gb|AAM63046.1 unknown {Arabidopsis
           thaliana}, partial (81%)
          Length = 723

 Score = 35.4 bits (80), Expect = 0.008
 Identities = 27/105 (25%), Positives = 49/105 (45%), Gaps = 25/105 (23%)
 Frame = +2

Query: 60  PELERDLREFLAFVEEV------------------QELSRLELKLLKEKERLEEKLKTAE 101
           P L++  ++F +FV EV                  QEL+  E+ LLK K  +E  ++  +
Sbjct: 242 PPLKKLQKKFTSFVSEVDKDEDNYNDCEKLARAFLQELTTFEIPLLKSKAIVEANVREKD 421

Query: 102 EILE-RDELKDRLSNIQHALERLEKDRSE------QRQECRRMRR 139
              E +DE+  +++  Q  +E L+K   E       ++EC  +R+
Sbjct: 422 NFNELKDEMNRQIAQAQVDIEDLKKQLEESKVERRHKEECEAIRK 556


>AJ499220 similar to SP|Q09863|YAF Hypothetical protein C29E6.10c in
           chromosome I. [Fission yeast] {Schizosaccharomyces
           pombe}, partial (12%)
          Length = 518

 Score = 34.7 bits (78), Expect = 0.013
 Identities = 24/72 (33%), Positives = 43/72 (59%), Gaps = 4/72 (5%)
 Frame = -3

Query: 73  VEEVQELSRL----ELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDRS 128
           +EE++E +RL    ELK LKEKER + K +  ++  +++E + R    + A E+ ++   
Sbjct: 261 LEELEEENRLKEERELKKLKEKERKKAKNRALKQ--QKEEERARREAERLAEEKAQRAER 88

Query: 129 EQRQECRRMRRD 140
           E++ E  R RR+
Sbjct: 87  EKKLEEERKRRE 52


>TC86834 similar to PIR|T46158|T46158 hypothetical protein T4D2.50 -
           Arabidopsis thaliana, partial (77%)
          Length = 1222

 Score = 32.7 bits (73), Expect = 0.050
 Identities = 21/58 (36%), Positives = 34/58 (58%), Gaps = 4/58 (6%)
 Frame = +1

Query: 85  KLLKEKERLEEKLKTAEEILERDELKDRLS--NIQHALERLEKD--RSEQRQECRRMR 138
           KLL +K+  ++ L + E++  +  LKD L+  N Q A E L+K+    E R +CR +R
Sbjct: 382 KLLSDKDAYQQFLNSLEQVKIQTNLKDELAKENRQLAEENLQKEPRMMELRNQCRIIR 555


>TC77947 homologue to GP|10334491|emb|CAC10207. putative splicing factor
           {Cicer arietinum}, partial (17%)
          Length = 742

 Score = 31.6 bits (70), Expect = 0.11
 Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
 Frame = +3

Query: 88  KEKERLEEKLKTAEEILERDE--LKDRLSNIQHALERLEKDRSEQRQECRRMRR 139
           +EKER  E+ + ++   ERD    ++     + ++ R E+DR  +R+E  R RR
Sbjct: 15  REKERERERSRRSKSRSERDREIEREERERSRRSISRSERDREREREERDRSRR 176


>AL379805 similar to GP|5821153|dbj RNA binding protein {Homo sapiens},
           partial (0%)
          Length = 458

 Score = 31.2 bits (69), Expect = 0.14
 Identities = 25/82 (30%), Positives = 43/82 (51%), Gaps = 1/82 (1%)
 Frame = +3

Query: 53  LLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDR 112
           L E+   P+L  D R+  A+V  + ++ R   KLL E+E   E+     ++L     +DR
Sbjct: 81  LAEDGYGPDLIGDERD-RAYVNSLSKIERE--KLLLEREERREQELERRKLLRNTNERDR 251

Query: 113 LSNIQ-HALERLEKDRSEQRQE 133
            ++ Q  ALE ++ +R  +R E
Sbjct: 252 ENSRQAEALELMKTERERKRTE 317


>BF646001 homologue to GP|20303561|gb| putative kinase {Oryza sativa
           (japonica cultivar-group)}, partial (6%)
          Length = 667

 Score = 30.4 bits (67), Expect = 0.25
 Identities = 35/130 (26%), Positives = 67/130 (50%), Gaps = 4/130 (3%)
 Frame = +2

Query: 2   SHLEQFSVR-EKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRP 60
           + LE+ + R  +++    V +E+LRK   E++  + I   EG        + L +E++  
Sbjct: 269 AELEKEAARLRREVVESKVEIEKLRKIIDEKENKIEIVEKEG--------KELKQENVEM 424

Query: 61  ELE-RDLREFLAFVE--EVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQ 117
           E++ R+L   +  +E  EV+E S    K ++ +E ++EK+   E  +E +ELK  L   +
Sbjct: 425 EMKVRELERRIGVIEMKEVEENS----KRVRIEEEMKEKIYEKE--MEVEELKSMLMGKK 586

Query: 118 HALERLEKDR 127
              E+  KD+
Sbjct: 587 VEEEKWLKDK 616


>AW691010 similar to GP|8656003|gb| Contains similarity to formin binding
           protein 11 from Mus musculus gb|AF135439 and contains,
           partial (18%)
          Length = 655

 Score = 30.4 bits (67), Expect = 0.25
 Identities = 29/101 (28%), Positives = 49/101 (47%), Gaps = 8/101 (7%)
 Frame = +3

Query: 48  ELIRTLLEEDLRPEL-------ERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTA 100
           ++I + LEE L  E        +R+  E+  F+E   +  +   +  K ++RLE     A
Sbjct: 327 DMIESFLEELLNKERAKVLEERKRNTVEYRKFLESC-DFIKANTQYRKVQDRLE-----A 488

Query: 101 EEILERDELKDRLSNIQHALERLEKDRSEQRQ-ECRRMRRD 140
           +E   + E  DRL   Q  L  LEK+  EQ++ +   +R+D
Sbjct: 489 DERCSQLEKIDRLEIFQDYLRDLEKEEEEQKKIQKEELRKD 611


>CA989870 similar to GP|18201934|g kinesin-like protein heavy chain
           {Arabidopsis thaliana}, partial (9%)
          Length = 806

 Score = 29.6 bits (65), Expect = 0.42
 Identities = 22/76 (28%), Positives = 39/76 (50%)
 Frame = +2

Query: 51  RTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELK 110
           +TL+   + PE+   L E ++ ++  + ++ +EL   K  +          E  E  ELK
Sbjct: 62  KTLMFVHINPEINA-LGETISTLKFAERVASIELGAAKSNK----------ETGEIRELK 208

Query: 111 DRLSNIQHALERLEKD 126
           + +SNI+ ALER E +
Sbjct: 209 EEISNIKSALERKENE 256


>TC89483 similar to GP|18377662|gb|AAL66981.1 unknown protein {Arabidopsis
           thaliana}, partial (35%)
          Length = 1711

 Score = 29.6 bits (65), Expect = 0.42
 Identities = 26/79 (32%), Positives = 38/79 (47%), Gaps = 4/79 (5%)
 Frame = +1

Query: 61  ELERDL----REFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNI 116
           E ERD     RE    +EEV+    L+LK  + +ER +EK +  E+  E+D  + R   I
Sbjct: 205 EAERDRIRKEREQKRRIEEVERQFELQLKEWEYREREKEKERQYEKEKEKDRERKRRKEI 384

Query: 117 QHALERLEKDRSEQRQECR 135
              L   E D  + R+  R
Sbjct: 385 ---LYDEEDDDGDSRKRWR 432


>BG456449 EGAD|146422|15 vitellogenin {Anolis pulchellus}, partial (6%)
          Length = 678

 Score = 29.3 bits (64), Expect = 0.55
 Identities = 37/114 (32%), Positives = 50/114 (43%), Gaps = 15/114 (13%)
 Frame = +1

Query: 35  LNIRHPEGTTTIAELIRTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLE 94
           + ++ PE +TTI  L RTL   +   +L+  L EFLA + +   LS L L  L+    L 
Sbjct: 25  VTVQQPE-STTIESLKRTLYNVE---QLQSQLPEFLA-LSDPDHLSTLPL--LQRAHSLF 183

Query: 95  EKLKTAEEILE---------------RDELKDRLSNIQHALERLEKDRSEQRQE 133
              K    + E               + EL DRLS  Q  LERL     EQ Q+
Sbjct: 184 SLAKLTSTLFELKLKCRGINPNDHAFKSEL-DRLSVCQKRLERLPDLSEEQWQD 342


>TC84943 similar to PIR|E86306|E86306 Similar to tuftelin-interacting
           protein [imported] - Arabidopsis thaliana, partial (25%)
          Length = 1066

 Score = 28.9 bits (63), Expect = 0.72
 Identities = 23/88 (26%), Positives = 41/88 (46%), Gaps = 5/88 (5%)
 Frame = +2

Query: 60  PELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEIL-----ERDELKDRLS 114
           P L+ + +     V+     +R  LK  K+K + EEK  TAEE+L     E  E+  ++ 
Sbjct: 134 PALQTEKKTEPGVVQPTVGRTRSWLKRPKKKNKEEEKYVTAEELLASKQEEESEVVQKIY 313

Query: 115 NIQHALERLEKDRSEQRQECRRMRRDPP 142
           +++    R+  + S+   E +    D P
Sbjct: 314 DMRGPQVRVLTNLSDLNAEEKAKENDVP 397


>TC80239 similar to GP|7208779|emb|CAB76912.1 hypothetical protein {Cicer
           arietinum}, partial (43%)
          Length = 930

 Score = 28.9 bits (63), Expect = 0.72
 Identities = 27/84 (32%), Positives = 44/84 (52%)
 Frame = +3

Query: 55  EEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLS 114
           EE    E  R   E    ++  Q   + E   LKEK+RLEE ++ A+E L+R +     +
Sbjct: 51  EEKEPNEPVRTKEEDEQILKAEQARKKEEEAKLKEKKRLEE-IEKAKEALQRKKRNAEKA 227

Query: 115 NIQHALERLEKDRSEQRQECRRMR 138
             Q AL + +K+ +EQ+++ R  R
Sbjct: 228 Q-QRALYKAQKE-AEQKEKEREKR 293


>AJ499968 weakly similar to GP|23495961|gb| hypothetical protein {Plasmodium
           falciparum 3D7}, partial (19%)
          Length = 342

 Score = 28.5 bits (62), Expect = 0.94
 Identities = 25/80 (31%), Positives = 39/80 (48%)
 Frame = -3

Query: 51  RTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELK 110
           R  LE   + + ER+ +E     EE +E  RLE  +  EKER E + K  +E  E+  LK
Sbjct: 235 RLELERIAKEKEERERKE----KEEREERERLEAVVRIEKERKEAEEKALKEQEEKALLK 68

Query: 111 DRLSNIQHALERLEKDRSEQ 130
           + + + +   E   K   E+
Sbjct: 67  EGVIDKKEEEEPKSKATPEE 8


>TC85391 homologue to PIR|T06598|T06598 dnaK-type molecular chaperone BiP-A
           - soybean, partial (52%)
          Length = 1231

 Score = 28.5 bits (62), Expect = 0.94
 Identities = 23/99 (23%), Positives = 44/99 (44%)
 Frame = +1

Query: 5   EQFSVREKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRPELER 64
           E+F+  +KK+   I A   L    +         + +   +  + +   LE D + ++E 
Sbjct: 703 EEFAEEDKKVKERIDARNALETYVY---------NMKNQVSDKDKLADKLESDEKEKIET 855

Query: 65  DLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEI 103
            ++E L ++++ Q +         EKE  EEKLK  E +
Sbjct: 856 AVKEALEWLDDNQSV---------EKEEYEEKLKEVEAV 945


>BE998238 similar to PIR|T39202|T39 heat shock protein 90 homolog - fission
           yeast (Schizosaccharomyces pombe), partial (30%)
          Length = 654

 Score = 28.1 bits (61), Expect = 1.2
 Identities = 19/70 (27%), Positives = 38/70 (54%), Gaps = 9/70 (12%)
 Frame = -2

Query: 46  IAELIRTLLEEDLRPELERDLREFLAFVEEVQ------ELSRLELKLLKEKERLEEKL-- 97
           + + I  ++ ++   ++E D+   L  +EE++      E  RLELKL  ++E L+ K+  
Sbjct: 524 LGDEILAVVHDEHTADIELDIVALLLGLEEIERRTLRDEQDRLELKLTLDREVLDSKVLF 345

Query: 98  -KTAEEILER 106
              A+ ++ER
Sbjct: 344 PVVAQALVER 315


>BQ137028 similar to GP|4914334|gb|A F14N23.20 {Arabidopsis thaliana},
           partial (7%)
          Length = 687

 Score = 28.1 bits (61), Expect = 1.2
 Identities = 19/53 (35%), Positives = 32/53 (59%), Gaps = 1/53 (1%)
 Frame = +2

Query: 76  VQELSRLEL-KLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDR 127
           +++L R E  K + EKERLEE+ +    + + +E + R+   Q   ER+E+DR
Sbjct: 74  LKKLKRKERRKEIAEKERLEEEAR----LNDPEEERKRILLEQQEAERIERDR 220


>TC91751 similar to GP|2739382|gb|AAC14505.1| unknown protein {Arabidopsis
           thaliana}, partial (38%)
          Length = 1200

 Score = 28.1 bits (61), Expect = 1.2
 Identities = 21/80 (26%), Positives = 40/80 (49%)
 Frame = +2

Query: 51  RTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELK 110
           R L+E++L  + + ++ E+    E  ++     LK L   +RL E+LK     LER + +
Sbjct: 272 RNLVEQEL-DKADEEIPEYRKQAETAEQTKNQVLKELDSTKRLIEELKLN---LERAQTE 439

Query: 111 DRLSNIQHALERLEKDRSEQ 130
           ++ +     L +L  +  EQ
Sbjct: 440 EQQARQDSELAKLRVEEMEQ 499


>TC80245 similar to PIR|T48581|T48581 hypothetical protein T31B5.160 -
           Arabidopsis thaliana, partial (60%)
          Length = 895

 Score = 28.1 bits (61), Expect = 1.2
 Identities = 23/56 (41%), Positives = 30/56 (53%), Gaps = 3/56 (5%)
 Frame = +2

Query: 59  RPELERDLREFLAFVEEVQELSRLELKLLKEKER---LEEKLKTAEEILERDELKD 111
           R EL++ L E    VEE Q    LEL+  KE+ER   LE   +  EE   R +L+D
Sbjct: 521 REELDKMLEENRRRVEESQRREALELQ-RKEEERQRELEMIRRQKEEAARRKKLED 685


>TC82291 similar to PIR|D96519|D96519 mysoin-like protein  11013-7318
           [imported] - Arabidopsis thaliana, partial (3%)
          Length = 1566

 Score = 28.1 bits (61), Expect = 1.2
 Identities = 22/82 (26%), Positives = 36/82 (43%), Gaps = 4/82 (4%)
 Frame = +3

Query: 61  ELERDLREFLAFVEEVQELSRLELKLLKE-KERLEEKL---KTAEEILERDELKDRLSNI 116
           +L+ D   F      +  L RLEL+ LKE   RLE+++   K+    L+    +  L   
Sbjct: 579 KLQADTSRFQELENTIANL-RLELQTLKESNRRLEDQIQNEKSLSRYLDTQLTETELKEA 755

Query: 117 QHALERLEKDRSEQRQECRRMR 138
            H +  LE +   +   C  +R
Sbjct: 756 YHKILALEVELESKNHFCEEIR 821


>TC85389 homologue to SP|Q03684|BIP4_TOBAC Luminal binding protein 4 precursor
            (BiP 4) (78 kDa glucose-regulated protein homolog 4) (GRP
            78-4)., partial (94%)
          Length = 2265

 Score = 28.1 bits (61), Expect = 1.2
 Identities = 23/99 (23%), Positives = 44/99 (44%)
 Frame = +2

Query: 5    EQFSVREKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRPELER 64
            E+F+  +KK+   I A   L    +         + +   +  + +   LE D + ++E 
Sbjct: 1712 EEFAEEDKKVKERIDARNALETYVY---------NMKNQISDKDKLADKLESDEKEKIEA 1864

Query: 65   DLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEI 103
             ++E L ++++ Q +         EKE  EEKLK  E +
Sbjct: 1865 AVKEALEWLDDNQTV---------EKEEFEEKLKEVEAV 1954


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.316    0.135    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,647,035
Number of Sequences: 36976
Number of extensions: 21873
Number of successful extensions: 222
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 219
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of query: 142
length of database: 9,014,727
effective HSP length: 87
effective length of query: 55
effective length of database: 5,797,815
effective search space: 318879825
effective search space used: 318879825
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)


Lotus: description of TM0142.1