
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0142.1
(142 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC93896 similar to GP|21553965|gb|AAM63046.1 unknown {Arabidopsi... 35 0.008
AJ499220 similar to SP|Q09863|YAF Hypothetical protein C29E6.10c... 35 0.013
TC86834 similar to PIR|T46158|T46158 hypothetical protein T4D2.5... 33 0.050
TC77947 homologue to GP|10334491|emb|CAC10207. putative splicing... 32 0.11
AL379805 similar to GP|5821153|dbj RNA binding protein {Homo sap... 31 0.14
BF646001 homologue to GP|20303561|gb| putative kinase {Oryza sat... 30 0.25
AW691010 similar to GP|8656003|gb| Contains similarity to formin... 30 0.25
CA989870 similar to GP|18201934|g kinesin-like protein heavy cha... 30 0.42
TC89483 similar to GP|18377662|gb|AAL66981.1 unknown protein {Ar... 30 0.42
BG456449 EGAD|146422|15 vitellogenin {Anolis pulchellus}, partia... 29 0.55
TC84943 similar to PIR|E86306|E86306 Similar to tuftelin-interac... 29 0.72
TC80239 similar to GP|7208779|emb|CAB76912.1 hypothetical protei... 29 0.72
AJ499968 weakly similar to GP|23495961|gb| hypothetical protein ... 28 0.94
TC85391 homologue to PIR|T06598|T06598 dnaK-type molecular chape... 28 0.94
BE998238 similar to PIR|T39202|T39 heat shock protein 90 homolog... 28 1.2
BQ137028 similar to GP|4914334|gb|A F14N23.20 {Arabidopsis thali... 28 1.2
TC91751 similar to GP|2739382|gb|AAC14505.1| unknown protein {Ar... 28 1.2
TC80245 similar to PIR|T48581|T48581 hypothetical protein T31B5.... 28 1.2
TC82291 similar to PIR|D96519|D96519 mysoin-like protein 11013-... 28 1.2
TC85389 homologue to SP|Q03684|BIP4_TOBAC Luminal binding protei... 28 1.2
>TC93896 similar to GP|21553965|gb|AAM63046.1 unknown {Arabidopsis
thaliana}, partial (81%)
Length = 723
Score = 35.4 bits (80), Expect = 0.008
Identities = 27/105 (25%), Positives = 49/105 (45%), Gaps = 25/105 (23%)
Frame = +2
Query: 60 PELERDLREFLAFVEEV------------------QELSRLELKLLKEKERLEEKLKTAE 101
P L++ ++F +FV EV QEL+ E+ LLK K +E ++ +
Sbjct: 242 PPLKKLQKKFTSFVSEVDKDEDNYNDCEKLARAFLQELTTFEIPLLKSKAIVEANVREKD 421
Query: 102 EILE-RDELKDRLSNIQHALERLEKDRSE------QRQECRRMRR 139
E +DE+ +++ Q +E L+K E ++EC +R+
Sbjct: 422 NFNELKDEMNRQIAQAQVDIEDLKKQLEESKVERRHKEECEAIRK 556
>AJ499220 similar to SP|Q09863|YAF Hypothetical protein C29E6.10c in
chromosome I. [Fission yeast] {Schizosaccharomyces
pombe}, partial (12%)
Length = 518
Score = 34.7 bits (78), Expect = 0.013
Identities = 24/72 (33%), Positives = 43/72 (59%), Gaps = 4/72 (5%)
Frame = -3
Query: 73 VEEVQELSRL----ELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDRS 128
+EE++E +RL ELK LKEKER + K + ++ +++E + R + A E+ ++
Sbjct: 261 LEELEEENRLKEERELKKLKEKERKKAKNRALKQ--QKEEERARREAERLAEEKAQRAER 88
Query: 129 EQRQECRRMRRD 140
E++ E R RR+
Sbjct: 87 EKKLEEERKRRE 52
>TC86834 similar to PIR|T46158|T46158 hypothetical protein T4D2.50 -
Arabidopsis thaliana, partial (77%)
Length = 1222
Score = 32.7 bits (73), Expect = 0.050
Identities = 21/58 (36%), Positives = 34/58 (58%), Gaps = 4/58 (6%)
Frame = +1
Query: 85 KLLKEKERLEEKLKTAEEILERDELKDRLS--NIQHALERLEKD--RSEQRQECRRMR 138
KLL +K+ ++ L + E++ + LKD L+ N Q A E L+K+ E R +CR +R
Sbjct: 382 KLLSDKDAYQQFLNSLEQVKIQTNLKDELAKENRQLAEENLQKEPRMMELRNQCRIIR 555
>TC77947 homologue to GP|10334491|emb|CAC10207. putative splicing factor
{Cicer arietinum}, partial (17%)
Length = 742
Score = 31.6 bits (70), Expect = 0.11
Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = +3
Query: 88 KEKERLEEKLKTAEEILERDE--LKDRLSNIQHALERLEKDRSEQRQECRRMRR 139
+EKER E+ + ++ ERD ++ + ++ R E+DR +R+E R RR
Sbjct: 15 REKERERERSRRSKSRSERDREIEREERERSRRSISRSERDREREREERDRSRR 176
>AL379805 similar to GP|5821153|dbj RNA binding protein {Homo sapiens},
partial (0%)
Length = 458
Score = 31.2 bits (69), Expect = 0.14
Identities = 25/82 (30%), Positives = 43/82 (51%), Gaps = 1/82 (1%)
Frame = +3
Query: 53 LLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDR 112
L E+ P+L D R+ A+V + ++ R KLL E+E E+ ++L +DR
Sbjct: 81 LAEDGYGPDLIGDERD-RAYVNSLSKIERE--KLLLEREERREQELERRKLLRNTNERDR 251
Query: 113 LSNIQ-HALERLEKDRSEQRQE 133
++ Q ALE ++ +R +R E
Sbjct: 252 ENSRQAEALELMKTERERKRTE 317
>BF646001 homologue to GP|20303561|gb| putative kinase {Oryza sativa
(japonica cultivar-group)}, partial (6%)
Length = 667
Score = 30.4 bits (67), Expect = 0.25
Identities = 35/130 (26%), Positives = 67/130 (50%), Gaps = 4/130 (3%)
Frame = +2
Query: 2 SHLEQFSVR-EKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRP 60
+ LE+ + R +++ V +E+LRK E++ + I EG + L +E++
Sbjct: 269 AELEKEAARLRREVVESKVEIEKLRKIIDEKENKIEIVEKEG--------KELKQENVEM 424
Query: 61 ELE-RDLREFLAFVE--EVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQ 117
E++ R+L + +E EV+E S K ++ +E ++EK+ E +E +ELK L +
Sbjct: 425 EMKVRELERRIGVIEMKEVEENS----KRVRIEEEMKEKIYEKE--MEVEELKSMLMGKK 586
Query: 118 HALERLEKDR 127
E+ KD+
Sbjct: 587 VEEEKWLKDK 616
>AW691010 similar to GP|8656003|gb| Contains similarity to formin binding
protein 11 from Mus musculus gb|AF135439 and contains,
partial (18%)
Length = 655
Score = 30.4 bits (67), Expect = 0.25
Identities = 29/101 (28%), Positives = 49/101 (47%), Gaps = 8/101 (7%)
Frame = +3
Query: 48 ELIRTLLEEDLRPEL-------ERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTA 100
++I + LEE L E +R+ E+ F+E + + + K ++RLE A
Sbjct: 327 DMIESFLEELLNKERAKVLEERKRNTVEYRKFLESC-DFIKANTQYRKVQDRLE-----A 488
Query: 101 EEILERDELKDRLSNIQHALERLEKDRSEQRQ-ECRRMRRD 140
+E + E DRL Q L LEK+ EQ++ + +R+D
Sbjct: 489 DERCSQLEKIDRLEIFQDYLRDLEKEEEEQKKIQKEELRKD 611
>CA989870 similar to GP|18201934|g kinesin-like protein heavy chain
{Arabidopsis thaliana}, partial (9%)
Length = 806
Score = 29.6 bits (65), Expect = 0.42
Identities = 22/76 (28%), Positives = 39/76 (50%)
Frame = +2
Query: 51 RTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELK 110
+TL+ + PE+ L E ++ ++ + ++ +EL K + E E ELK
Sbjct: 62 KTLMFVHINPEINA-LGETISTLKFAERVASIELGAAKSNK----------ETGEIRELK 208
Query: 111 DRLSNIQHALERLEKD 126
+ +SNI+ ALER E +
Sbjct: 209 EEISNIKSALERKENE 256
>TC89483 similar to GP|18377662|gb|AAL66981.1 unknown protein {Arabidopsis
thaliana}, partial (35%)
Length = 1711
Score = 29.6 bits (65), Expect = 0.42
Identities = 26/79 (32%), Positives = 38/79 (47%), Gaps = 4/79 (5%)
Frame = +1
Query: 61 ELERDL----REFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNI 116
E ERD RE +EEV+ L+LK + +ER +EK + E+ E+D + R I
Sbjct: 205 EAERDRIRKEREQKRRIEEVERQFELQLKEWEYREREKEKERQYEKEKEKDRERKRRKEI 384
Query: 117 QHALERLEKDRSEQRQECR 135
L E D + R+ R
Sbjct: 385 ---LYDEEDDDGDSRKRWR 432
>BG456449 EGAD|146422|15 vitellogenin {Anolis pulchellus}, partial (6%)
Length = 678
Score = 29.3 bits (64), Expect = 0.55
Identities = 37/114 (32%), Positives = 50/114 (43%), Gaps = 15/114 (13%)
Frame = +1
Query: 35 LNIRHPEGTTTIAELIRTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLE 94
+ ++ PE +TTI L RTL + +L+ L EFLA + + LS L L L+ L
Sbjct: 25 VTVQQPE-STTIESLKRTLYNVE---QLQSQLPEFLA-LSDPDHLSTLPL--LQRAHSLF 183
Query: 95 EKLKTAEEILE---------------RDELKDRLSNIQHALERLEKDRSEQRQE 133
K + E + EL DRLS Q LERL EQ Q+
Sbjct: 184 SLAKLTSTLFELKLKCRGINPNDHAFKSEL-DRLSVCQKRLERLPDLSEEQWQD 342
>TC84943 similar to PIR|E86306|E86306 Similar to tuftelin-interacting
protein [imported] - Arabidopsis thaliana, partial (25%)
Length = 1066
Score = 28.9 bits (63), Expect = 0.72
Identities = 23/88 (26%), Positives = 41/88 (46%), Gaps = 5/88 (5%)
Frame = +2
Query: 60 PELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEIL-----ERDELKDRLS 114
P L+ + + V+ +R LK K+K + EEK TAEE+L E E+ ++
Sbjct: 134 PALQTEKKTEPGVVQPTVGRTRSWLKRPKKKNKEEEKYVTAEELLASKQEEESEVVQKIY 313
Query: 115 NIQHALERLEKDRSEQRQECRRMRRDPP 142
+++ R+ + S+ E + D P
Sbjct: 314 DMRGPQVRVLTNLSDLNAEEKAKENDVP 397
>TC80239 similar to GP|7208779|emb|CAB76912.1 hypothetical protein {Cicer
arietinum}, partial (43%)
Length = 930
Score = 28.9 bits (63), Expect = 0.72
Identities = 27/84 (32%), Positives = 44/84 (52%)
Frame = +3
Query: 55 EEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLS 114
EE E R E ++ Q + E LKEK+RLEE ++ A+E L+R + +
Sbjct: 51 EEKEPNEPVRTKEEDEQILKAEQARKKEEEAKLKEKKRLEE-IEKAKEALQRKKRNAEKA 227
Query: 115 NIQHALERLEKDRSEQRQECRRMR 138
Q AL + +K+ +EQ+++ R R
Sbjct: 228 Q-QRALYKAQKE-AEQKEKEREKR 293
>AJ499968 weakly similar to GP|23495961|gb| hypothetical protein {Plasmodium
falciparum 3D7}, partial (19%)
Length = 342
Score = 28.5 bits (62), Expect = 0.94
Identities = 25/80 (31%), Positives = 39/80 (48%)
Frame = -3
Query: 51 RTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELK 110
R LE + + ER+ +E EE +E RLE + EKER E + K +E E+ LK
Sbjct: 235 RLELERIAKEKEERERKE----KEEREERERLEAVVRIEKERKEAEEKALKEQEEKALLK 68
Query: 111 DRLSNIQHALERLEKDRSEQ 130
+ + + + E K E+
Sbjct: 67 EGVIDKKEEEEPKSKATPEE 8
>TC85391 homologue to PIR|T06598|T06598 dnaK-type molecular chaperone BiP-A
- soybean, partial (52%)
Length = 1231
Score = 28.5 bits (62), Expect = 0.94
Identities = 23/99 (23%), Positives = 44/99 (44%)
Frame = +1
Query: 5 EQFSVREKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRPELER 64
E+F+ +KK+ I A L + + + + + + LE D + ++E
Sbjct: 703 EEFAEEDKKVKERIDARNALETYVY---------NMKNQVSDKDKLADKLESDEKEKIET 855
Query: 65 DLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEI 103
++E L ++++ Q + EKE EEKLK E +
Sbjct: 856 AVKEALEWLDDNQSV---------EKEEYEEKLKEVEAV 945
>BE998238 similar to PIR|T39202|T39 heat shock protein 90 homolog - fission
yeast (Schizosaccharomyces pombe), partial (30%)
Length = 654
Score = 28.1 bits (61), Expect = 1.2
Identities = 19/70 (27%), Positives = 38/70 (54%), Gaps = 9/70 (12%)
Frame = -2
Query: 46 IAELIRTLLEEDLRPELERDLREFLAFVEEVQ------ELSRLELKLLKEKERLEEKL-- 97
+ + I ++ ++ ++E D+ L +EE++ E RLELKL ++E L+ K+
Sbjct: 524 LGDEILAVVHDEHTADIELDIVALLLGLEEIERRTLRDEQDRLELKLTLDREVLDSKVLF 345
Query: 98 -KTAEEILER 106
A+ ++ER
Sbjct: 344 PVVAQALVER 315
>BQ137028 similar to GP|4914334|gb|A F14N23.20 {Arabidopsis thaliana},
partial (7%)
Length = 687
Score = 28.1 bits (61), Expect = 1.2
Identities = 19/53 (35%), Positives = 32/53 (59%), Gaps = 1/53 (1%)
Frame = +2
Query: 76 VQELSRLEL-KLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDR 127
+++L R E K + EKERLEE+ + + + +E + R+ Q ER+E+DR
Sbjct: 74 LKKLKRKERRKEIAEKERLEEEAR----LNDPEEERKRILLEQQEAERIERDR 220
>TC91751 similar to GP|2739382|gb|AAC14505.1| unknown protein {Arabidopsis
thaliana}, partial (38%)
Length = 1200
Score = 28.1 bits (61), Expect = 1.2
Identities = 21/80 (26%), Positives = 40/80 (49%)
Frame = +2
Query: 51 RTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELK 110
R L+E++L + + ++ E+ E ++ LK L +RL E+LK LER + +
Sbjct: 272 RNLVEQEL-DKADEEIPEYRKQAETAEQTKNQVLKELDSTKRLIEELKLN---LERAQTE 439
Query: 111 DRLSNIQHALERLEKDRSEQ 130
++ + L +L + EQ
Sbjct: 440 EQQARQDSELAKLRVEEMEQ 499
>TC80245 similar to PIR|T48581|T48581 hypothetical protein T31B5.160 -
Arabidopsis thaliana, partial (60%)
Length = 895
Score = 28.1 bits (61), Expect = 1.2
Identities = 23/56 (41%), Positives = 30/56 (53%), Gaps = 3/56 (5%)
Frame = +2
Query: 59 RPELERDLREFLAFVEEVQELSRLELKLLKEKER---LEEKLKTAEEILERDELKD 111
R EL++ L E VEE Q LEL+ KE+ER LE + EE R +L+D
Sbjct: 521 REELDKMLEENRRRVEESQRREALELQ-RKEEERQRELEMIRRQKEEAARRKKLED 685
>TC82291 similar to PIR|D96519|D96519 mysoin-like protein 11013-7318
[imported] - Arabidopsis thaliana, partial (3%)
Length = 1566
Score = 28.1 bits (61), Expect = 1.2
Identities = 22/82 (26%), Positives = 36/82 (43%), Gaps = 4/82 (4%)
Frame = +3
Query: 61 ELERDLREFLAFVEEVQELSRLELKLLKE-KERLEEKL---KTAEEILERDELKDRLSNI 116
+L+ D F + L RLEL+ LKE RLE+++ K+ L+ + L
Sbjct: 579 KLQADTSRFQELENTIANL-RLELQTLKESNRRLEDQIQNEKSLSRYLDTQLTETELKEA 755
Query: 117 QHALERLEKDRSEQRQECRRMR 138
H + LE + + C +R
Sbjct: 756 YHKILALEVELESKNHFCEEIR 821
>TC85389 homologue to SP|Q03684|BIP4_TOBAC Luminal binding protein 4 precursor
(BiP 4) (78 kDa glucose-regulated protein homolog 4) (GRP
78-4)., partial (94%)
Length = 2265
Score = 28.1 bits (61), Expect = 1.2
Identities = 23/99 (23%), Positives = 44/99 (44%)
Frame = +2
Query: 5 EQFSVREKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRPELER 64
E+F+ +KK+ I A L + + + + + + LE D + ++E
Sbjct: 1712 EEFAEEDKKVKERIDARNALETYVY---------NMKNQISDKDKLADKLESDEKEKIEA 1864
Query: 65 DLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEI 103
++E L ++++ Q + EKE EEKLK E +
Sbjct: 1865 AVKEALEWLDDNQTV---------EKEEFEEKLKEVEAV 1954
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.135 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,647,035
Number of Sequences: 36976
Number of extensions: 21873
Number of successful extensions: 222
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 219
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of query: 142
length of database: 9,014,727
effective HSP length: 87
effective length of query: 55
effective length of database: 5,797,815
effective search space: 318879825
effective search space used: 318879825
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0142.1