BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0136.17
(83 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC93652 Enod8.2 [Medicago truncatula] 111 6e-26
TC76859 Enod8.1 [Medicago truncatula]; early nodule-specific pro... 109 2e-25
TC81963 Enod8-like protein [Medicago truncatula] 101 6e-23
AW688035 similar to PIR|T48618|T486 early nodule-specific protei... 82 5e-17
TC84309 similar to GP|9294302|dbj|BAB02204.1 nodulin-like protei... 80 1e-16
TC83147 similar to PIR|T48618|T48618 early nodule-specific prote... 80 1e-16
AW692346 similar to GP|11072007|gb| F12A21.4 {Arabidopsis thalia... 77 1e-15
TC83609 similar to GP|5295941|dbj|BAA81842.1 ESTs AU075322(C1110... 76 2e-15
TC90006 similar to PIR|A96590|A96590 hypothetical protein T22H22... 69 4e-13
TC82515 similar to GP|3688284|emb|CAA09694.1 lanatoside 15'-O-ac... 68 7e-13
TC79343 weakly similar to PIR|E86411|E86411 protein F1K23.18 [im... 66 2e-12
BG587016 similar to PIR|T09416|T09 coil protein PO22 microspore... 60 2e-10
TC83671 similar to PIR|T09416|T09416 coil protein PO22 microspo... 56 3e-09
TC90156 similar to PIR|B86227|B86227 hypothetical protein [impor... 54 8e-09
TC79836 similar to GP|13430834|gb|AAK26039.1 unknown protein {Ar... 43 2e-05
AW691634 similar to GP|13430834|gb| unknown protein {Arabidopsis... 42 3e-05
TC83941 similar to PIR|E96579|E96579 hypothetical protein T18A20... 42 4e-05
TC86678 similar to PIR|E96579|E96579 hypothetical protein T18A20... 42 4e-05
TC91899 similar to GP|21358785|gb|AAM47031.1 lipase SIL1 {Brassi... 40 2e-04
TC89134 weakly similar to GP|13430834|gb|AAK26039.1 unknown prot... 37 0.001
>TC93652 Enod8.2 [Medicago truncatula]
Length = 1195
Score = 111 bits (277), Expect = 6e-26
Identities = 52/83 (62%), Positives = 61/83 (72%)
Frame = +3
Query: 1 MSLMEFHRHVSFVSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKS 60
M E RH+S VSL V++ P+FA + C+FPAIF+ GASN DTGGLAAAF P S
Sbjct: 33 MEKFELLRHMSLVSLIVLILCIITPPIFATRNCDFPAIFSFGASNVDTGGLAAAFQAPPS 212
Query: 61 PYGDTYFHRPAGRYSDGRIILDF 83
PYG+TYFHR GR+SDGRIILDF
Sbjct: 213 PYGETYFHRSTGRFSDGRIILDF 281
>TC76859 Enod8.1 [Medicago truncatula]; early nodule-specific protein
Length = 1350
Score = 109 bits (272), Expect = 2e-25
Identities = 52/83 (62%), Positives = 63/83 (75%)
Frame = +2
Query: 1 MSLMEFHRHVSFVSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKS 60
M+ +E RH+ V+L V++ T P+FA K C+FPAIF+ GASN DTGGLAAAF P S
Sbjct: 47 MAKIELSRHIPLVTLIVLVLCIT-PPIFATKNCDFPAIFSFGASNVDTGGLAAAFRAPPS 223
Query: 61 PYGDTYFHRPAGRYSDGRIILDF 83
PYG+TYFHR GR+SDGRIILDF
Sbjct: 224 PYGETYFHRSTGRFSDGRIILDF 292
>TC81963 Enod8-like protein [Medicago truncatula]
Length = 783
Score = 101 bits (251), Expect = 6e-23
Identities = 48/77 (62%), Positives = 59/77 (76%), Gaps = 2/77 (2%)
Frame = +3
Query: 9 HVSFVSLFVILF--TTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTY 66
H+S V+ FV+L TTT+ + CNF AIFN G SNSDTGGLAA+F+ PK PYG+TY
Sbjct: 99 HISSVTFFVVLSIATTTVIESSSNSECNFRAIFNFGDSNSDTGGLAASFVAPKPPYGETY 278
Query: 67 FHRPAGRYSDGRIILDF 83
FHRP GR+SDGR+I+DF
Sbjct: 279 FHRPNGRFSDGRLIVDF 329
>AW688035 similar to PIR|T48618|T486 early nodule-specific protein-like -
Arabidopsis thaliana, partial (13%)
Length = 596
Score = 81.6 bits (200), Expect = 5e-17
Identities = 35/51 (68%), Positives = 43/51 (83%)
Frame = +2
Query: 33 CNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAGRYSDGRIILDF 83
C+FPAI+N G SNSDTGG++AAF+P SPYG +FH+P GR SDGR+ILDF
Sbjct: 83 CSFPAIYNFGDSNSDTGGISAAFVPIPSPYGQGFFHKPFGRDSDGRVILDF 235
>TC84309 similar to GP|9294302|dbj|BAB02204.1 nodulin-like protein protein
{Arabidopsis thaliana}, partial (47%)
Length = 672
Score = 80.5 bits (197), Expect = 1e-16
Identities = 38/71 (53%), Positives = 50/71 (69%)
Frame = +2
Query: 13 VSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAG 72
+S F+++ +PV + C+FPAIFN G SNSDTGGL+AAF P G T+F PAG
Sbjct: 62 ISTFLVVVMVP-SPVIGARNCSFPAIFNFGDSNSDTGGLSAAFGQAPPPNGITFFQTPAG 238
Query: 73 RYSDGRIILDF 83
R+SDGR+I+DF
Sbjct: 239 RFSDGRLIIDF 271
>TC83147 similar to PIR|T48618|T48618 early nodule-specific protein-like -
Arabidopsis thaliana, partial (43%)
Length = 653
Score = 80.5 bits (197), Expect = 1e-16
Identities = 34/51 (66%), Positives = 43/51 (83%)
Frame = +1
Query: 33 CNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAGRYSDGRIILDF 83
C+FPAI+N G SNSDTGG++AAF+P SPYG +FH+P GR SDGR+ILD+
Sbjct: 82 CSFPAIYNFGDSNSDTGGISAAFVPIPSPYGQGFFHKPFGRDSDGRVILDY 234
>AW692346 similar to GP|11072007|gb| F12A21.4 {Arabidopsis thaliana}, partial
(12%)
Length = 356
Score = 77.0 bits (188), Expect = 1e-15
Identities = 34/55 (61%), Positives = 40/55 (71%)
Frame = +1
Query: 23 TLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAGRYSDG 77
T N V ++C FPAIFN G SNSDTGGL+AAF P PYG+++FH P GRY DG
Sbjct: 190 TDNSVSGSRKCEFPAIFNFGDSNSDTGGLSAAFGQPGYPYGESFFHHPVGRYCDG 354
>TC83609 similar to GP|5295941|dbj|BAA81842.1 ESTs AU075322(C11109)
D22430(C11109) correspond to a region of the predicted
gene.~Similar to, partial (22%)
Length = 437
Score = 76.3 bits (186), Expect = 2e-15
Identities = 35/71 (49%), Positives = 46/71 (64%)
Frame = +2
Query: 13 VSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAG 72
+ LF+ + ++ C+F AIFN G SNSDTGG AAF PYG TYF++PAG
Sbjct: 131 ILLFIFMLVLPCLVGLSQGECDFKAIFNFGDSNSDTGGFYAAFPAESGPYGMTYFNKPAG 310
Query: 73 RYSDGRIILDF 83
R SDGR+++DF
Sbjct: 311 RASDGRLVIDF 343
>TC90006 similar to PIR|A96590|A96590 hypothetical protein T22H22.20
[imported] - Arabidopsis thaliana, partial (63%)
Length = 858
Score = 68.6 bits (166), Expect = 4e-13
Identities = 36/70 (51%), Positives = 43/70 (61%), Gaps = 1/70 (1%)
Frame = +3
Query: 15 LFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAA-FLPPKSPYGDTYFHRPAGR 73
+ I+ T L P +FPA+FN G SNSDTG L A F P G TYFH P+GR
Sbjct: 57 IICIITTIFLLPCAKSIHLDFPAVFNFGDSNSDTGTLVTAGFESLYPPNGHTYFHLPSGR 236
Query: 74 YSDGRIILDF 83
YSDGR+I+DF
Sbjct: 237 YSDGRLIIDF 266
>TC82515 similar to GP|3688284|emb|CAA09694.1 lanatoside
15'-O-acetylesterase {Digitalis lanata}, partial (76%)
Length = 1230
Score = 67.8 bits (164), Expect = 7e-13
Identities = 31/52 (59%), Positives = 36/52 (68%)
Frame = +1
Query: 32 RCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAGRYSDGRIILDF 83
+C+F IFN G SNSDTGG +AF PYG TYF P GR SDGR+I+DF
Sbjct: 130 KCDFQGIFNFGDSNSDTGGFYSAFPAQPIPYGMTYFKTPVGRSSDGRLIVDF 285
>TC79343 weakly similar to PIR|E86411|E86411 protein F1K23.18 [imported] -
Arabidopsis thaliana, partial (52%)
Length = 1420
Score = 66.2 bits (160), Expect = 2e-12
Identities = 34/78 (43%), Positives = 47/78 (59%), Gaps = 5/78 (6%)
Frame = +2
Query: 11 SFVSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGGLAAAFLPPKS-----PYGDT 65
S V+L ++L T P+F ++ +IF+ G S +DTG L + PP PYG T
Sbjct: 119 STVTLQLLLVITVSAPLFTAACSSYSSIFSFGDSIADTGNLYLSSQPPSDHCFFPPYGQT 298
Query: 66 YFHRPAGRYSDGRIILDF 83
YFH P+GR SDGR+I+DF
Sbjct: 299 YFHHPSGRCSDGRLIIDF 352
>BG587016 similar to PIR|T09416|T09 coil protein PO22
microspore/pollen-specific - alfalfa, partial (55%)
Length = 696
Score = 59.7 bits (143), Expect = 2e-10
Identities = 27/53 (50%), Positives = 34/53 (63%)
Frame = +3
Query: 31 KRCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAGRYSDGRIILDF 83
K+C +PAI+N G SNSDTG A + P G +YF GR SDGR+I+DF
Sbjct: 114 KKCEYPAIYNFGDSNSDTGAANAIYTAVTPPNGISYFGSTTGRASDGRLIIDF 272
>TC83671 similar to PIR|T09416|T09416 coil protein PO22
microspore/pollen-specific - alfalfa, partial (60%)
Length = 671
Score = 55.8 bits (133), Expect = 3e-09
Identities = 25/51 (49%), Positives = 35/51 (68%)
Frame = +3
Query: 32 RCNFPAIFNLGASNSDTGGLAAAFLPPKSPYGDTYFHRPAGRYSDGRIILD 82
+C +PAI+N G SNSDTG + A + + P G T+F +GR SDGR+I+D
Sbjct: 48 KCVYPAIYNFGDSNSDTGTIYATYTSVQPPNGITFFGNISGRASDGRLIID 200
>TC90156 similar to PIR|B86227|B86227 hypothetical protein [imported] -
Arabidopsis thaliana, partial (84%)
Length = 1421
Score = 54.3 bits (129), Expect = 8e-09
Identities = 31/78 (39%), Positives = 48/78 (60%), Gaps = 4/78 (5%)
Frame = +3
Query: 10 VSFVSLFVILFTTTLNPVFA-KKRCNF-PAI-FNLGASNSDTGGLAAAF-LPPKSPYGDT 65
V ++S+ + +F ++++ + C+ PA+ F G SNSDTGGL + P P G T
Sbjct: 51 VLWISMTICIFFSSVSLALSVSSGCSSKPAVVFVFGDSNSDTGGLVSGLGFPVNLPNGRT 230
Query: 66 YFHRPAGRYSDGRIILDF 83
+FHR GR SDGR+++DF
Sbjct: 231 FFHRSTGRLSDGRLVIDF 284
>TC79836 similar to GP|13430834|gb|AAK26039.1 unknown protein {Arabidopsis
thaliana}, partial (57%)
Length = 1366
Score = 42.7 bits (99), Expect = 2e-05
Identities = 29/86 (33%), Positives = 48/86 (55%), Gaps = 5/86 (5%)
Frame = +3
Query: 3 LMEFHRHVSFVSLFVILFTTTLNPVFAK--KRCNFPA--IFNLGASNSDTGGLAAAFLPP 58
+M+ H+ + F+ L++ L L+ + A+ + +P +F G S DTG +
Sbjct: 141 IMDSHKQL-FILLWLSLVLVLLSGLQAEASRHLKYPTKELFVFGDSYVDTGNSQKSKPGS 317
Query: 59 -KSPYGDTYFHRPAGRYSDGRIILDF 83
K PYG T+ +PAGR+SDGR++ DF
Sbjct: 318 WKEPYGITFPGKPAGRFSDGRVLTDF 395
>AW691634 similar to GP|13430834|gb| unknown protein {Arabidopsis thaliana},
partial (16%)
Length = 387
Score = 42.4 bits (98), Expect = 3e-05
Identities = 23/50 (46%), Positives = 33/50 (66%), Gaps = 4/50 (8%)
Frame = +3
Query: 38 IFNLGASNSDTG----GLAAAFLPPKSPYGDTYFHRPAGRYSDGRIILDF 83
+F G S +DTG GLA ++ K PYG T+ +PAGR+SDGR++ D+
Sbjct: 213 LFVFGDSYADTGNIKRGLANSW---KHPYGITFPGKPAGRFSDGRVLTDY 353
>TC83941 similar to PIR|E96579|E96579 hypothetical protein T18A20.15
[imported] - Arabidopsis thaliana, partial (25%)
Length = 684
Score = 42.0 bits (97), Expect = 4e-05
Identities = 26/79 (32%), Positives = 38/79 (47%), Gaps = 5/79 (6%)
Frame = +3
Query: 10 VSFVSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGG-----LAAAFLPPKSPYGD 64
+ F+ +++ T L + K A+F G S D G L PYG+
Sbjct: 66 IFFLCYGILISTQCLGNICVPKE--HVALFVFGDSFFDVGNNNYINTTTDLLANYPPYGE 239
Query: 65 TYFHRPAGRYSDGRIILDF 83
T+F P+GR+SDGR+I DF
Sbjct: 240 TFFKYPSGRFSDGRVIPDF 296
>TC86678 similar to PIR|E96579|E96579 hypothetical protein T18A20.15
[imported] - Arabidopsis thaliana, partial (43%)
Length = 1341
Score = 42.0 bits (97), Expect = 4e-05
Identities = 32/85 (37%), Positives = 41/85 (47%), Gaps = 14/85 (16%)
Frame = +2
Query: 13 VSLF--VILFTTTLNPVFAKKRCNF---PAIFNLGASNSDTGGL---------AAAFLPP 58
VS+F V L ++ F K + A+F G S D G A FLP
Sbjct: 95 VSIFFKVFLIIAIISQTFGSKTDYYRSNKALFIFGDSFLDAGNNNYINTTTFDQANFLP- 271
Query: 59 KSPYGDTYFHRPAGRYSDGRIILDF 83
YG+TYF+ P GR+SDGR+I DF
Sbjct: 272 ---YGETYFNFPTGRFSDGRLISDF 337
>TC91899 similar to GP|21358785|gb|AAM47031.1 lipase SIL1 {Brassica rapa
subsp. pekinensis}, partial (29%)
Length = 699
Score = 39.7 bits (91), Expect = 2e-04
Identities = 26/79 (32%), Positives = 38/79 (47%), Gaps = 5/79 (6%)
Frame = +2
Query: 10 VSFVSLFVILFTTTLNPVFAKKRCNFPAIFNLGASNSDTGG-----LAAAFLPPKSPYGD 64
+ F+S +++ T L + K A+F G S D G + SPYG+
Sbjct: 62 IFFISYGMLIPTQCLGDICLPKE--HVALFIFGDSLFDVGNNNYINTTTDYQANFSPYGE 235
Query: 65 TYFHRPAGRYSDGRIILDF 83
T+F GR+SDGR+I DF
Sbjct: 236 TFFKFSTGRFSDGRVIPDF 292
>TC89134 weakly similar to GP|13430834|gb|AAK26039.1 unknown protein
{Arabidopsis thaliana}, partial (48%)
Length = 1122
Score = 37.0 bits (84), Expect = 0.001
Identities = 18/38 (47%), Positives = 24/38 (62%), Gaps = 1/38 (2%)
Frame = +2
Query: 47 DTGG-LAAAFLPPKSPYGDTYFHRPAGRYSDGRIILDF 83
DTG + F K PYG T+ +P GR+SDGR++ DF
Sbjct: 8 DTGNSIKTIFDSWKVPYGITFPGKPVGRFSDGRVLTDF 121
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.328 0.143 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,824,351
Number of Sequences: 36976
Number of extensions: 35205
Number of successful extensions: 324
Number of sequences better than 10.0: 82
Number of HSP's better than 10.0 without gapping: 321
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 321
length of query: 83
length of database: 9,014,727
effective HSP length: 59
effective length of query: 24
effective length of database: 6,833,143
effective search space: 163995432
effective search space used: 163995432
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0136.17