BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0134.5
(510 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC76981 similar to PIR|T12198|T12198 sucrose transport protein -... 533 e-152
BG450712 similar to GP|17645762|em unnamed protein product {Glyc... 303 1e-82
TC89981 similar to GP|9957218|gb|AAG09270.1| sucrose transporter... 293 1e-79
TC76983 similar to PIR|T12198|T12198 sucrose transport protein -... 227 6e-76
AW773728 similar to GP|10998390|gb sucrose transporter SUT4 {Sol... 251 5e-67
TC79556 similar to GP|5882292|gb|AAD55269.1| sucrose transporter... 228 4e-60
TC76982 similar to PIR|T12198|T12198 sucrose transport protein -... 227 9e-60
BF520636 homologue to GP|5230818|gb|A sucrose transport protein ... 89 5e-18
CB894752 36 0.031
CA918296 GP|20385063|gb serine/threonine protein phosphatase 2A ... 31 1.3
AW684300 30 2.9
TC79129 similar to PIR|A96725|A96725 hypothetical protein F20P5.... 30 2.9
TC93881 similar to GP|9081779|dbj|BAA99518.1 hypothetical protei... 29 3.8
TC91200 weakly similar to GP|10177640|dbj|BAB10787. contains sim... 28 6.4
BQ147250 28 6.4
AJ496898 similar to GP|23171295|gb CG3731-PA {Drosophila melanog... 28 8.4
TC79272 similar to GP|15011484|gb|AAK77579.1 ymf63 {Tetrahymena ... 28 8.4
TC77026 homologue to GP|14036786|emb|CAC38728. RKS10 cDNA {Arabi... 28 8.4
>TC76981 similar to PIR|T12198|T12198 sucrose transport protein - fava bean,
partial (71%)
Length = 1224
Score = 533 bits (1372), Expect = e-152
Identities = 267/388 (68%), Positives = 308/388 (78%), Gaps = 14/388 (3%)
Frame = +2
Query: 3 SPTKSNHLDTKG---SSLQIEAHIPQQSSPLRKMIAVASIAAGIQFGWALQLSLLTPYVQ 59
S TK NH + SL +E+ P + SPLRK+I VASIAAG+QFGWALQLSLLTPYVQ
Sbjct: 50 SSTKQNHNNNNTLTKPSLHVESP-PLEPSPLRKIIVVASIAAGVQFGWALQLSLLTPYVQ 226
Query: 60 TLGVPHIWASFIWLCGPVSGLLVQPIVGYYSDRTTCRFGRRRPFILAGAVAVAISVFLIG 119
LG+PH WA++IWLCGP+SG+LVQPIVGY+SDR T RFGRRRPFI AG+ AVAI+VFLIG
Sbjct: 227 LLGIPHTWAAYIWLCGPISGMLVQPIVGYHSDRCTSRFGRRRPFIAAGSFAVAIAVFLIG 406
Query: 120 YAADIGHSMGDDLAKKTRPRAVAIFVFGFWILDVANNMLQGPCRAFLGDLAAGDQKKTRT 179
YAAD+GHS GDDL+KK RPRA+ IFV GFWILDVANNMLQGPCRA LGDL AG+ +KTR
Sbjct: 407 YAADLGHSFGDDLSKKVRPRAIGIFVVGFWILDVANNMLQGPCRALLGDLCAGNHQKTRN 586
Query: 180 AMGFFSFFMAVGNVLGYAAGAYSGLHKIFPFTVTDACPSFCANLKSCFFFSILLLLILSI 239
A FFSFFMAVGN+LGYAAGAYS L +FPFT T AC +CANLKSCFF SI LL ++
Sbjct: 587 ANAFFSFFMAVGNILGYAAGAYSKLFHVFPFTKTKACDIYCANLKSCFFLSIALLTAVAT 766
Query: 240 AALIYVEDTPL-----TKKPAADVDSPVS-----CFGDLFGAFKELKKPMWILMLVTAVN 289
AALIYV++ PL T D D V+ CFG+L GAF+ELK+PMWIL+LVT +N
Sbjct: 767 AALIYVKEIPLSPEKVTGNGVTDEDGNVTKSSNPCFGELSGAFRELKRPMWILLLVTCLN 946
Query: 290 WVAWFPFFLFDTDWMGREVYGGVVGE-KAYDDGVRAGSLGLMINAVVLAFMSLAVEPLGR 348
W+AWFPF LFDTDWMG+EVYGG VGE AYD GVRAG+LGLM+N+VVL SL V+ L R
Sbjct: 947 WIAWFPFLLFDTDWMGKEVYGGTVGEGHAYDKGVRAGALGLMLNSVVLGATSLXVDVLAR 1126
Query: 349 ILGGVKNLWGIVNFILAICMAMTVLITK 376
+GGVK LWGIVNF+LAIC+AMTV TK
Sbjct: 1127GVGGVKRLWGIVNFLLAICLAMTVXATK 1210
>BG450712 similar to GP|17645762|em unnamed protein product {Glycine max},
partial (35%)
Length = 555
Score = 303 bits (776), Expect = 1e-82
Identities = 144/184 (78%), Positives = 160/184 (86%)
Frame = +2
Query: 27 SSPLRKMIAVASIAAGIQFGWALQLSLLTPYVQTLGVPHIWASFIWLCGPVSGLLVQPIV 86
++PLRKM AVASIAAGIQFGWALQLSLLTPYVQ LGVPH WA+ IWLCGP+SG+++QP+V
Sbjct: 2 ATPLRKMAAVASIAAGIQFGWALQLSLLTPYVQLLGVPHQWAANIWLCGPISGMIIQPLV 181
Query: 87 GYYSDRTTCRFGRRRPFILAGAVAVAISVFLIGYAADIGHSMGDDLAKKTRPRAVAIFVF 146
GYYSDR+ RFGRRRPFI GA+AVAI+VFLIGYAAD+GHSMGDDL KKTRPRAV IFVF
Sbjct: 182 GYYSDRSHSRFGRRRPFIFFGAIAVAIAVFLIGYAADLGHSMGDDLTKKTRPRAVVIFVF 361
Query: 147 GFWILDVANNMLQGPCRAFLGDLAAGDQKKTRTAMGFFSFFMAVGNVLGYAAGAYSGLHK 206
GFWILDVANNMLQGPCRAF+GDLA GD + R G FSFFMA GNVLGYAAG+Y L+
Sbjct: 362 GFWILDVANNMLQGPCRAFIGDLAGGDHRXMRIGNGMFSFFMAXGNVLGYAAGSYXKLYT 541
Query: 207 IFPF 210
PF
Sbjct: 542 KXPF 553
>TC89981 similar to GP|9957218|gb|AAG09270.1| sucrose transporter
{Lycopersicon esculentum}, partial (39%)
Length = 717
Score = 293 bits (749), Expect = 1e-79
Identities = 141/232 (60%), Positives = 172/232 (73%), Gaps = 5/232 (2%)
Frame = +3
Query: 1 MESPTKSN-HLDTKGSSLQIEAHIP----QQSSPLRKMIAVASIAAGIQFGWALQLSLLT 55
M +PT +N H SS P Q +PLR+++ VAS+A+GIQFGWALQLSLLT
Sbjct: 21 MPNPTTTNPHRSRTRSSTSTSTSRPVQPVQPRTPLRQLLRVASVASGIQFGWALQLSLLT 200
Query: 56 PYVQTLGVPHIWASFIWLCGPVSGLLVQPIVGYYSDRTTCRFGRRRPFILAGAVAVAISV 115
PYVQ LG+PH WAS IWLCGPVSGL VQP+VG+ SDR + RFGRRRPFIL GA ++ ++V
Sbjct: 201 PYVQQLGIPHKWASIIWLCGPVSGLFVQPLVGHLSDRCSSRFGRRRPFILVGAASIVVAV 380
Query: 116 FLIGYAADIGHSMGDDLAKKTRPRAVAIFVFGFWILDVANNMLQGPCRAFLGDLAAGDQK 175
+IGYAADIG+ +GDD+ + RP A+ +FV GFWILDVANN+ QGPCRA L DL D +
Sbjct: 381 VIIGYAADIGYLIGDDITQNYRPFAIVVFVIGFWILDVANNVTQGPCRALLADLTCNDAR 560
Query: 176 KTRTAMGFFSFFMAVGNVLGYAAGAYSGLHKIFPFTVTDACPSFCANLKSCF 227
+TR A +FS FMAVGN+LGYA G+YSG +KIF FT+T AC CAN KS F
Sbjct: 561 RTRVANAYFSLFMAVGNILGYATGSYSGWYKIFTFTLTPACSIXCANXKSAF 716
>TC76983 similar to PIR|T12198|T12198 sucrose transport protein - fava bean,
partial (40%)
Length = 674
Score = 227 bits (579), Expect(2) = 6e-76
Identities = 109/140 (77%), Positives = 121/140 (85%)
Frame = +2
Query: 76 PVSGLLVQPIVGYYSDRTTCRFGRRRPFILAGAVAVAISVFLIGYAADIGHSMGDDLAKK 135
P+SG+LVQPIVGY+SDR T RFGRRRPFI AG+ AVAI+VFLIGYAAD+GHS GDDL+KK
Sbjct: 227 PISGMLVQPIVGYHSDRCTSRFGRRRPFIAAGSFAVAIAVFLIGYAADLGHSFGDDLSKK 406
Query: 136 TRPRAVAIFVFGFWILDVANNMLQGPCRAFLGDLAAGDQKKTRTAMGFFSFFMAVGNVLG 195
RPRA+ IFV GFWILDVANNMLQGPCRA LGDL AG+ +KTR A FFSFFMAVGN+LG
Sbjct: 407 VRPRAIGIFVVGFWILDVANNMLQGPCRALLGDLCAGNHQKTRNANAFFSFFMAVGNILG 586
Query: 196 YAAGAYSGLHKIFPFTVTDA 215
YAAGAYS L +FPFT T A
Sbjct: 587 YAAGAYSKLFHVFPFTKTKA 646
Score = 75.5 bits (184), Expect(2) = 6e-76
Identities = 41/65 (63%), Positives = 48/65 (73%), Gaps = 3/65 (4%)
Frame = +3
Query: 3 SPTKSNHLDTKG---SSLQIEAHIPQQSSPLRKMIAVASIAAGIQFGWALQLSLLTPYVQ 59
S TK NH + SL +E+ P + SPLRK+I VASIAAG+QFGWALQLSLLTPYVQ
Sbjct: 36 SSTKQNHNNNNTLTKPSLHVESP-PLEPSPLRKIIVVASIAAGVQFGWALQLSLLTPYVQ 212
Query: 60 TLGVP 64
LG+P
Sbjct: 213LLGIP 227
>AW773728 similar to GP|10998390|gb sucrose transporter SUT4 {Solanum
tuberosum}, partial (34%)
Length = 620
Score = 251 bits (641), Expect = 5e-67
Identities = 121/181 (66%), Positives = 141/181 (77%)
Frame = +2
Query: 24 PQQSSPLRKMIAVASIAAGIQFGWALQLSLLTPYVQTLGVPHIWASFIWLCGPVSGLLVQ 83
P+ L K++ VAS+A GIQFGWALQLSLLTPYVQ LG+PH WAS IWLCGP+SGLLVQ
Sbjct: 74 PKDRVLLTKLLRVASVAGGIQFGWALQLSLLTPYVQQLGIPHAWASIIWLCGPLSGLLVQ 253
Query: 84 PIVGYYSDRTTCRFGRRRPFILAGAVAVAISVFLIGYAADIGHSMGDDLAKKTRPRAVAI 143
P+VG+ SDR T RFGRRRPFIL GAV++ ISV +IG+AAD+G GD K R AVA
Sbjct: 254 PLVGHLSDRCTSRFGRRRPFILGGAVSIVISVLIIGHAADLGWKFGD--TKNHRHSAVAF 427
Query: 144 FVFGFWILDVANNMLQGPCRAFLGDLAAGDQKKTRTAMGFFSFFMAVGNVLGYAAGAYSG 203
FVFGFWILDVANN+ QGPCRA LGDL D + TR A +FS FMA+GN+LG+A G+YS
Sbjct: 428 FVFGFWILDVANNVTQGPCRALLGDLTGKDHRMTRVANAYFSLFMAIGNILGFATGSYSW 607
Query: 204 L 204
L
Sbjct: 608 L 610
>TC79556 similar to GP|5882292|gb|AAD55269.1| sucrose transporter {Vitis
vinifera}, partial (45%)
Length = 1209
Score = 228 bits (581), Expect = 4e-60
Identities = 119/232 (51%), Positives = 155/232 (66%), Gaps = 4/232 (1%)
Frame = +3
Query: 267 DLFGAFKELKKPMWILMLVTAVNWVAWFPFFLFDTDWMGREVYGG-VVGEKAYDDGVRAG 325
+LFG FK P+WI++ VTA+ W+ WFPF LFDTDWMGRE+YGG G YD GVR G
Sbjct: 54 ELFGTFKYFSMPVWIVLSVTALTWIGWFPFNLFDTDWMGREIYGGDPEGGLIYDTGVRMG 233
Query: 326 SLGLMINAVVLAFMSLAVEPLGRILGGVKNLWGIVNFILAICMAMTVLITKTAEHDRLIS 385
+LGL++N+VVLA SL +E L R G +WGI N +AIC +++T A +S
Sbjct: 234 ALGLLLNSVVLAVTSLLMERLCR-KRGAGFVWGISNIFMAICFIAMLVLTYAANSIGYVS 410
Query: 386 GGATVGAPSSGVKAGAIAFFGVMGIPLAINFSVPFALASIYSSAAGAGQGLSLGVLNLAI 445
G P +G+ A+A F ++G P+AI +SVP+AL S + G GQGLS+GVLNLAI
Sbjct: 411 KGQP---PPTGIVIAALAIFTILGFPMAITYSVPYALISTHIEPLGLGQGLSMGVLNLAI 581
Query: 446 VVPQMLVSTLSGPWDALFGGGNLPAFVVGAVMAAVSAIMAIVLLP---TPKP 494
VVPQ++VS SGPWD LFGGGN PAF V AV A +S ++A++ +P T KP
Sbjct: 582 VVPQIVVSLGSGPWDQLFGGGNSPAFAVAAVAALLSGLLALLAIPRTRTQKP 737
>TC76982 similar to PIR|T12198|T12198 sucrose transport protein - fava bean,
partial (40%)
Length = 1085
Score = 227 bits (578), Expect = 9e-60
Identities = 119/179 (66%), Positives = 142/179 (78%), Gaps = 2/179 (1%)
Frame = +2
Query: 334 VVLAFMSLAVEPLGRILGGVKNLWGIVNFILAICMAMTVLITKTAEHDRLISGGATVGA- 392
VVL SL V+ L R +GGVK LWGIVNF LAIC+AMTVL+TK A+H R+ + +
Sbjct: 140 VVLGATSLGVDVLARGVGGVKRLWGIVNFPLAICLAMTVLVTKLAQHSRVYADASHTDPL 319
Query: 393 -PSSGVKAGAIAFFGVMGIPLAINFSVPFALASIYSSAAGAGQGLSLGVLNLAIVVPQML 451
PS G+ AGA+A F V+GIPLAI +S+PFALASI+SS++GAGQGLSLGVLNLAIV+PQM+
Sbjct: 320 PPSGGITAGALALFSVLGIPLAITYSIPFALASIFSSSSGAGQGLSLGVLNLAIVIPQMI 499
Query: 452 VSTLSGPWDALFGGGNLPAFVVGAVMAAVSAIMAIVLLPTPKPADVAKASSLPPGGGFH 510
VS LSGPWDALFGGGNLPAFVVGAV A S I+++VLLP+P P D+AK S GGGFH
Sbjct: 500 VSVLSGPWDALFGGGNLPAFVVGAVAALASGILSVVLLPSP-PPDLAK-SVTATGGGFH 670
>BF520636 homologue to GP|5230818|gb|A sucrose transport protein SUT1 {Pisum
sativum}, partial (16%)
Length = 334
Score = 88.6 bits (218), Expect = 5e-18
Identities = 46/81 (56%), Positives = 57/81 (69%), Gaps = 2/81 (2%)
Frame = +1
Query: 334 VVLAFMSLAVEPLGRILGGVKNLWGIVNFILAICMAMTVLITKTAEHDRLISGGATVG-- 391
VVL SL V+ L R +GGVK LWGIVNF LAIC+AMTVL+TK A+H R+ + +
Sbjct: 67 VVLGATSLGVDVLARGVGGVKRLWGIVNFPLAICLAMTVLVTKLAQHSRVYADASHTDPF 246
Query: 392 APSSGVKAGAIAFFGVMGIPL 412
PS G+ AGA+A F V+GIPL
Sbjct: 247 PPSGGITAGALALFSVLGIPL 309
>CB894752
Length = 152
Score = 36.2 bits (82), Expect = 0.031
Identities = 20/49 (40%), Positives = 29/49 (58%)
Frame = +1
Query: 188 MAVGNVLGYAAGAYSGLHKIFPFTVTDACPSFCANLKSCFFFSILLLLI 236
MAVG ++GYA G++SG KI +T A AN KS FF + +++
Sbjct: 1 MAVGTIVGYALGSFSGWDKIRTPILTVASFISGANHKSAFFPDVAAIVV 147
>CA918296 GP|20385063|gb serine/threonine protein phosphatase 2A {Pisum
sativum}, partial (32%)
Length = 717
Score = 30.8 bits (68), Expect = 1.3
Identities = 18/58 (31%), Positives = 29/58 (49%)
Frame = +3
Query: 220 CANLKSCFFFSILLLLILSIAALIYVEDTPLTKKPAADVDSPVSCFGDLFGAFKELKK 277
C N F FS+ L L + + V++ + + V+SPV+ GD+ G F +L K
Sbjct: 414 CFNCV*LFLFSLSLKLK*LLL*MFQVKEILIEESNVQPVNSPVTVCGDIHGQFHDLMK 587
>AW684300
Length = 442
Score = 29.6 bits (65), Expect = 2.9
Identities = 15/39 (38%), Positives = 25/39 (63%)
Frame = +2
Query: 205 HKIFPFTVTDACPSFCANLKSCFFFSILLLLILSIAALI 243
HKI PF+ T S +N+ S FFF +L L+ +S++ ++
Sbjct: 35 HKITPFSFTKKKTSQISNVSS-FFFLLLTLITISLSLIL 148
>TC79129 similar to PIR|A96725|A96725 hypothetical protein F20P5.7
[imported] - Arabidopsis thaliana, partial (64%)
Length = 1328
Score = 29.6 bits (65), Expect = 2.9
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 134 KKTRPRAVAIFVFGFWILDVANNMLQGPCRAFL 166
++T P + I GFWILD+ N + G C +L
Sbjct: 423 RRTSPLTIWI---GFWILDLYRNQMDGHCNFYL 512
>TC93881 similar to GP|9081779|dbj|BAA99518.1 hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (27%)
Length = 826
Score = 29.3 bits (64), Expect = 3.8
Identities = 16/46 (34%), Positives = 23/46 (49%)
Frame = -2
Query: 219 FCANLKSCFFFSILLLLILSIAALIYVEDTPLTKKPAADVDSPVSC 264
F + SCFF IL L+L + + PLT AA + + +SC
Sbjct: 537 FALAIASCFFGIILPALLLPF--VFFFSFLPLTASTAASISASISC 406
>TC91200 weakly similar to GP|10177640|dbj|BAB10787. contains similarity to
heparanase~gene_id:MGG23.2 {Arabidopsis thaliana},
partial (15%)
Length = 805
Score = 28.5 bits (62), Expect = 6.4
Identities = 17/61 (27%), Positives = 33/61 (53%)
Frame = +3
Query: 275 LKKPMWILMLVTAVNWVAWFPFFLFDTDWMGREVYGGVVGEKAYDDGVRAGSLGLMINAV 334
L+ WI +L ++ ++AWF FF+ +T V E YD+ V+ G++ + ++V
Sbjct: 342 LEMGSWIQIL--SIYYLAWFSFFVVNT----------VSDESGYDEDVKKGNVVIDGSSV 485
Query: 335 V 335
+
Sbjct: 486 I 488
>BQ147250
Length = 628
Score = 28.5 bits (62), Expect = 6.4
Identities = 22/57 (38%), Positives = 27/57 (46%), Gaps = 3/57 (5%)
Frame = +2
Query: 225 SCFFFSILLLLILSIAALI-YVEDTP--LTKKPAADVDSPVSCFGDLFGAFKELKKP 278
S SIL + + IA Y+ + P LTK V + CFG L FK LKKP
Sbjct: 188 STILLSILAVAVFHIATTXXYIXNVPSFLTKSHXIIVHF-LXCFGKLCQXFKYLKKP 355
>AJ496898 similar to GP|23171295|gb CG3731-PA {Drosophila melanogaster},
partial (35%)
Length = 698
Score = 28.1 bits (61), Expect = 8.4
Identities = 13/32 (40%), Positives = 19/32 (58%)
Frame = +2
Query: 447 VPQMLVSTLSGPWDALFGGGNLPAFVVGAVMA 478
+P M+ +TL G WD GGG A + A++A
Sbjct: 62 IPLMVANTLLGAWDRSQGGGANNASGLAAIVA 157
>TC79272 similar to GP|15011484|gb|AAK77579.1 ymf63 {Tetrahymena
thermophila}, partial (6%)
Length = 687
Score = 28.1 bits (61), Expect = 8.4
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +2
Query: 211 TVTDACPSFCANLKSCFFFSILLLLILSIAALIYVEDT 248
++T + P+F A L S F +I LL+ + A IY+ T
Sbjct: 116 SLTSSSPTFWAGLYSWFTPTIFFLLLQLVIATIYITST 229
>TC77026 homologue to GP|14036786|emb|CAC38728. RKS10 cDNA {Arabidopsis
thaliana}, partial (61%)
Length = 1434
Score = 28.1 bits (61), Expect = 8.4
Identities = 14/29 (48%), Positives = 16/29 (54%)
Frame = +1
Query: 385 SGGATVGAPSSGVKAGAIAFFGVMGIPLA 413
SGG+ GA + GV AGA F I LA
Sbjct: 7 SGGSNTGAIAGGVAAGAALLFAAPAIALA 93
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.141 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,038,702
Number of Sequences: 36976
Number of extensions: 237540
Number of successful extensions: 1845
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1818
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1835
length of query: 510
length of database: 9,014,727
effective HSP length: 100
effective length of query: 410
effective length of database: 5,317,127
effective search space: 2180022070
effective search space used: 2180022070
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0134.5