BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0134.24
(366 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-li... 148 3e-36
TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imp... 138 4e-33
BE248682 similar to GP|18568269|gb putative gag-pol polyprotein ... 127 7e-30
BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgari... 77 8e-15
BG647708 weakly similar to GP|13786450|gb| putative reverse tran... 63 2e-10
BQ148771 62 5e-10
TC91765 weakly similar to GP|19881779|gb|AAM01180.1 Putative ret... 45 2e-07
CB891696 47 2e-05
TC83624 homologue to PIR|G84581|G84581 copia-like retroelement p... 42 5e-04
TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarot... 42 5e-04
BF006686 41 8e-04
BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [impo... 35 0.035
TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non... 35 0.060
TC82948 33 0.13
AW774658 similar to GP|2808681|emb| Hcr9-4B {Lycopersicon hirsut... 26 0.69
TC90516 30 1.5
TC87263 similar to GP|9759298|dbj|BAB09804.1 emb|CAB82953.1~gene... 30 1.5
TC87567 similar to GP|17065112|gb|AAL32710.1 Unknown protein {Ar... 29 3.3
TC77553 homologue to GP|1066499|gb|AAB41904.1| NADH-dependent gl... 29 3.3
TC82555 similar to PIR|T46017|T46017 hypothetical protein T10K17... 29 3.3
>BG586266 similar to GP|7267666|em RNA-directed DNA polymerase-like protein
{Arabidopsis thaliana}, partial (18%)
Length = 789
Score = 148 bits (374), Expect = 3e-36
Identities = 88/237 (37%), Positives = 134/237 (56%), Gaps = 7/237 (2%)
Frame = -3
Query: 1 KLLSKRMGRVLGEVIDESQSAFIGGRHMLDSVLVANEVVDEARRQGKE---CMIFKVDYK 57
K+LSKRM +L +I SQSAF+ GR + D+VL+ ++++ R+ G + M K D
Sbjct: 724 KILSKRMQPLLRSIISPSQSAFVPGRAISDNVLITHKILHYLRQSGAKKHVSMAVKTDMT 545
Query: 58 KAYDSVRSDFLYYMMRRLGFCEKWIGWIKACIQSATVSVLVNGSPTEESVMQRGLRQGDP 117
KAYD + +FL ++ RLGF WI WI C+ + + S L+NG P + RGLRQGDP
Sbjct: 544 KAYDRIAWNFLREVLTRLGFHGIWISWIMECVSTVSYSFLINGGPQGRVLPSRGLRQGDP 365
Query: 118 LAPFLFLVVAEWLSGLMRQARRLNLFKGYKVGMDGMEISLLQFLDDTLFIGEASTQNVLV 177
L+P+LF++ E LSGL +QA R G KV + I+ L F DDT+F G+++ + +
Sbjct: 364 LSPYLFILCTEVLSGLCQQALRKGTLPGVKVARNCPPINHLLFADDTMFFGKSNASSCAI 185
Query: 178 LKGMLRYFELLSGLKVNFSKNRFPGISLGEGTTQCFARML--NCRVMKEPFT--YLG 230
L ++ + SG +N +K+ I+ T+Q + ++ KE T YLG
Sbjct: 184 LLSIMDKYRAASGRCIN*TKS---AITFSSKTSQAIIDRVKGELKIAKEGGTGKYLG 23
>TC83437 weakly similar to PIR|D86384|D86384 unknown protein [imported] -
Arabidopsis thaliana, partial (6%)
Length = 951
Score = 138 bits (347), Expect = 4e-33
Identities = 92/285 (32%), Positives = 144/285 (50%), Gaps = 2/285 (0%)
Frame = +2
Query: 1 KLLSKRMGRVLGEVIDESQSAFIGGRHMLDSVLVANEVVDEARRQGKECMIFKVDYKKAY 60
KLL+ R+ V+G VI ++QSAF+ R +L+ V + + R ++ K
Sbjct: 155 KLLANRLRVVIGSVISDAQSAFVKNRQILEMVFL*QMRLWMRLRN*RKIFCCLRWILKRL 334
Query: 61 DSVRSDFLYYMM-RRLGFCEKWIGWIKACIQSATVSVLVNGSPTEESVMQRGLRQGDPLA 119
++ ++ + + F W WIK C+ +AT SVLVNGSPT
Sbjct: 335 ITLSIGLIWILF*VGMSFLVLWRKWIKECVSTATTSVLVNGSPTNV-------------- 472
Query: 120 PFLFLVVAEWLSGLMRQARRLNLFKGYKVGM-DGMEISLLQFLDDTLFIGEASTQNVLVL 178
LM+ + LF Y G+ + + +S LQF +DTL + + N+ L
Sbjct: 473 -------------LMKSLVQTQLFTRYSFGVVNPVVVSHLQFANDTLLLETKNWANIRAL 613
Query: 179 KGMLRYFELLSGLKVNFSKNRFPGISLGEGTTQCFARMLNCRVMKEPFTYLGIFVGANPR 238
+ L F +SGLKVNF K+ +++ A +L+ +V K PF YLG+ + N R
Sbjct: 614 RAALVIF*AMSGLKVNFHKSGLVCVNIAPSWLSEAASVLSWKVGKVPFLYLGMPIEGNSR 793
Query: 239 RRSMWEPLVCKMKKRLQSWKQKMLSFGGKVCLIKSVLSSIPLFYL 283
R S WEP+V ++K RL W + LSFGG++ L+KSVL+S+ ++ L
Sbjct: 794 RLSFWEPIVNRIKARLTGWNSRFLSFGGRLVLLKSVLTSLSVYAL 928
>BE248682 similar to GP|18568269|gb putative gag-pol polyprotein {Zea mays},
partial (1%)
Length = 441
Score = 127 bits (319), Expect = 7e-30
Identities = 66/128 (51%), Positives = 85/128 (65%)
Frame = +3
Query: 104 EESVMQRGLRQGDPLAPFLFLVVAEWLSGLMRQARRLNLFKGYKVGMDGMEISLLQFLDD 163
EE +QRGL+QGDPLAPFLFL+VAE +SGLM+ A NLF+G+ V G +S LQ+ DD
Sbjct: 6 EEISVQRGLKQGDPLAPFLFLLVAEGISGLMKNAVNRNLFQGFDVKRGGTRVSHLQYADD 185
Query: 164 TLFIGEASTQNVLVLKGMLRYFELLSGLKVNFSKNRFPGISLGEGTTQCFARMLNCRVMK 223
TL IG + N+ LK +L+ FE+ SGLKVNF K+ GI++ + R LNCR
Sbjct: 186 TLCIGMPTVDNLWTLKALLQGFEMASGLKVNFHKSSLIGINVPRDFMEAACRFLNCREES 365
Query: 224 EPFTYLGI 231
PF YLG+
Sbjct: 366 IPFIYLGL 389
>BF650289 weakly similar to GP|9049283|dbj| orf129a {Beta vulgaris}, partial
(69%)
Length = 616
Score = 77.4 bits (189), Expect = 8e-15
Identities = 40/105 (38%), Positives = 66/105 (62%), Gaps = 2/105 (1%)
Frame = +3
Query: 1 KLLSKRMGRVLGEVIDESQSAFIGGRHMLDSVLVANEVVDEARRQG--KECMIFKVDYKK 58
K+L+ RM VL V+ E+QSAF+ GR + D++++++E+V R+G CM+ K+D K
Sbjct: 297 KILTSRMQGVLNSVVSENQSAFVKGRVIFDNIILSHELVKSYSRKGISPRCMV-KIDLXK 473
Query: 59 AYDSVRSDFLYYMMRRLGFCEKWIGWIKACIQSATVSVLVNGSPT 103
AYDS F+ ++M LGF K++ W+ A + +A+ + NG T
Sbjct: 474 AYDSXEWPFIKHLMLELGFPYKFVNWVMAXLTTASYTFNXNGDLT 608
>BG647708 weakly similar to GP|13786450|gb| putative reverse transcriptase
{Oryza sativa}, partial (9%)
Length = 708
Score = 63.2 bits (152), Expect = 2e-10
Identities = 32/90 (35%), Positives = 51/90 (56%)
Frame = +1
Query: 109 QRGLRQGDPLAPFLFLVVAEWLSGLMRQARRLNLFKGYKVGMDGMEISLLQFLDDTLFIG 168
++GLRQGDPL+P+LF++ A LSGL+++ G +V +I+ L F DD+L
Sbjct: 7 EKGLRQGDPLSPYLFILCANVLSGLLKREGNKQNLHGIQVARSDPKITHLLFADDSLLFA 186
Query: 169 EASTQNVLVLKGMLRYFELLSGLKVNFSKN 198
A+ + +L ++ SG VNF K+
Sbjct: 187 RANLTEAATIMQVLHSYQSASGQLVNFEKS 276
>BQ148771
Length = 680
Score = 61.6 bits (148), Expect = 5e-10
Identities = 33/113 (29%), Positives = 57/113 (50%)
Frame = -3
Query: 254 LQSWKQKMLSFGGKVCLIKSVLSSIPLFYLSFFKFPRGIAKECKRIQMDFLWGREVGAGK 313
L +WK LS +V L KSV+ ++PL+ + P+ +E +++Q F+WG + +
Sbjct: 573 LANWKANHLSLARRVTLAKSVIEAVPLYPMMTTIIPKACIEEIQKLQRKFVWGDTEVSRR 394
Query: 314 ICWAKWSTVCKIKEEEGLGVKDLERFNRALMSKWRWRNLGEEDRLWGEVLSSR 366
W T+ K K GLG++ L+ N+A + K W + L EV+ +
Sbjct: 393 YHAVGWETMSKPKTIYGLGLRRLDVMNKACIMKLGWSIYSGSNSLCTEVMRGK 235
>TC91765 weakly similar to GP|19881779|gb|AAM01180.1 Putative retroelement
{Oryza sativa (japonica cultivar-group)}, partial (1%)
Length = 625
Score = 44.7 bits (104), Expect(2) = 2e-07
Identities = 20/51 (39%), Positives = 32/51 (62%)
Frame = +2
Query: 88 CIQSATVSVLVNGSPTEESVMQRGLRQGDPLAPFLFLVVAEWLSGLMRQAR 138
C++S VLVN + + RGL+QGD L+P++F++ E LS L+ A+
Sbjct: 104 CVESNDYYVLVNNDAVDPIIPSRGLQQGDHLSPYIFIICVEGLSFLIPHAK 256
Score = 28.1 bits (61), Expect(2) = 2e-07
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +1
Query: 58 KAYDSVRSDFLYYMMRRLGFCEKWIGWI 85
K Y+ V D+L +M ++GF +WI W+
Sbjct: 13 KVYNRVD*DYLKEIMIKMGFNNRWIYWM 96
>CB891696
Length = 638
Score = 46.6 bits (109), Expect = 2e-05
Identities = 31/85 (36%), Positives = 45/85 (52%)
Frame = +1
Query: 173 QNVLVLKGMLRYFELLSGLKVNFSKNRFPGISLGEGTTQCFARMLNCRVMKEPFTYLGIF 232
+N+L +K ++ YFEL S L VNF K+ +++ + C+V F YLGI
Sbjct: 7 ENILTMKTIVSYFELASSLWVNFLKSGLINLNVIGHF*GW*NIYIKCKVH*VIFKYLGIL 186
Query: 233 VGANPRRRSMWEPLVCKMKKRLQSW 257
VG NP R +M E L+ + L SW
Sbjct: 187 VGENPCRVNM*ELLLKLLTN*LGSW 261
>TC83624 homologue to PIR|G84581|G84581 copia-like retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(1%)
Length = 831
Score = 41.6 bits (96), Expect = 5e-04
Identities = 19/53 (35%), Positives = 31/53 (57%)
Frame = +1
Query: 195 FSKNRFPGISLGEGTTQCFARMLNCRVMKEPFTYLGIFVGANPRRRSMWEPLV 247
FS+ F ++L E + L C V + PF +LG+ +GANP+R S +P++
Sbjct: 256 FSRVNFMALNLEESFVEASPNFLLCNVNEVPFCFLGLPIGANPKRSSTRKPVL 414
>TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarotenoid
dioxygenase1 {Pisum sativum}, partial (43%)
Length = 1865
Score = 41.6 bits (96), Expect = 5e-04
Identities = 18/41 (43%), Positives = 27/41 (64%)
Frame = -3
Query: 323 CKIKEEEGLGVKDLERFNRALMSKWRWRNLGEEDRLWGEVL 363
C + + GLGV+D+ N +L++KW WR L ++ LW EVL
Sbjct: 969 CLPRCKGGLGVRDIRLVNVSLLAKWWWRLLQDQSSLWKEVL 847
>BF006686
Length = 325
Score = 40.8 bits (94), Expect = 8e-04
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = +3
Query: 242 MWEPLVCKMKKRLQSWKQKMLSFGGKVCLI 271
MWEPL+ + K L+SW K+LSFGG++ L+
Sbjct: 234 MWEPLLEHVNKMLKSWGNKLLSFGGRIVLL 323
>BG447966 weakly similar to PIR|G96509|G96 protein F27F5.21 [imported] -
Arabidopsis thaliana, partial (7%)
Length = 687
Score = 35.4 bits (80), Expect = 0.035
Identities = 20/49 (40%), Positives = 31/49 (62%), Gaps = 2/49 (4%)
Frame = +2
Query: 1 KLLSKRMGRVLGEVIDESQSAFIGGRHMLDSVLVANEVVD--EARRQGK 47
K+++ R+ + L +VID QSAF+ GR + D+ L+A V RR+GK
Sbjct: 530 KVIANRVKQTLPDVIDVEQSAFVQGRLITDNALIAWSVSIG*RXRRKGK 676
>TC80819 weakly similar to GP|10140689|gb|AAG13524.1 putative non-LTR
retroelement reverse transcriptase {Oryza sativa
(japonica cultivar-group)}, partial (2%)
Length = 1262
Score = 34.7 bits (78), Expect = 0.060
Identities = 17/38 (44%), Positives = 24/38 (62%)
Frame = +1
Query: 329 EGLGVKDLERFNRALMSKWRWRNLGEEDRLWGEVLSSR 366
+GLGV FN +L+ KW WR L +++ LW VL +R
Sbjct: 28 KGLGVG---AFNLSLLGKWCWRLLVDKEGLWHRVLKAR 132
>TC82948
Length = 705
Score = 33.5 bits (75), Expect = 0.13
Identities = 14/38 (36%), Positives = 20/38 (51%)
Frame = +3
Query: 312 GKICWAKWSTVCKIKEEEGLGVKDLERFNRALMSKWRW 349
GK+ W VC+ +E LG++ L + N AL K W
Sbjct: 252 GKVVKVSWEKVCRPIKEGSLGIRSLSKLNEALNLKLCW 365
>AW774658 similar to GP|2808681|emb| Hcr9-4B {Lycopersicon hirsutum}, partial
(4%)
Length = 665
Score = 26.2 bits (56), Expect(2) = 0.69
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = -1
Query: 131 SGLMRQARRLNLFKGYKVGMDGMEI-SLLQFLDDTLFIGEASTQN 174
S L+ + LNL +GM + + S LQF DDTL +G S N
Sbjct: 449 SNLLSHSICLNL----SIGMHSLTVFSHLQFADDTLLLGVKSWAN 327
Score = 23.5 bits (49), Expect(2) = 0.69
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = -3
Query: 167 IGEASTQNVLVLKGMLRYFELLSGLKVN 194
+GE + +++LV+ FE +SGLKVN
Sbjct: 336 LGERALRSILVI------FENMSGLKVN 271
>TC90516
Length = 983
Score = 30.0 bits (66), Expect = 1.5
Identities = 29/118 (24%), Positives = 49/118 (40%), Gaps = 2/118 (1%)
Frame = -2
Query: 1 KLLSKRMGRVLGEVIDESQSAFIGGRHMLDSVLVANEVVDEARRQGKECMIFKVDYKKAY 60
K+L+ R+ ++ ++I +QS F+ GR +D V+ N E ++ D
Sbjct: 247 KVLALRLAHIMEKIIFVNQSTFVRGRQHVDGVVAIN-----------EIIVLMGD----- 116
Query: 61 DSVRSDFLYYMMRRLGFCEKWIGWI--KACIQSATVSVLVNGSPTEESVMQRGLRQGD 116
G + C+ ++LVN SPT E +Q+GL+QGD
Sbjct: 115 ----------------------GVVD*SVCVYK*P-AILVNCSPT*EIDIQKGLKQGD 11
>TC87263 similar to GP|9759298|dbj|BAB09804.1
emb|CAB82953.1~gene_id:MPH15.5~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (65%)
Length = 2229
Score = 30.0 bits (66), Expect = 1.5
Identities = 42/149 (28%), Positives = 61/149 (40%), Gaps = 30/149 (20%)
Frame = +2
Query: 231 IFVGANPRRRSMWEPLVCKMKKRLQSWKQKMLSFGGKV---------CLIKSVLSSIPLF 281
+FVG + R+MWE L+C +K ++ K+K+ G+V + K S+ LF
Sbjct: 1031 VFVG-DSLNRNMWESLICILKNSVKD-KKKVYEANGRVHFRGEASYSFVFKDYKFSVELF 1204
Query: 282 YLSF----FKFP--RGIAKECKRIQMDFLWGREVGAGKIC---------WAKWSTVCKIK 326
F ++ P G KE R+ L GR K W K K
Sbjct: 1205 VSPFLVQEWEMPDKNGTKKETLRLD---LVGRSSDQYKDADIIVFNTGHWWTHDKTSKGK 1375
Query: 327 E--EEGLGVKD----LERFNRALMSKWRW 349
+ +EG V D LE F RA+ + RW
Sbjct: 1376 DYYQEGSHVYDELNVLEAFRRAITTWGRW 1462
>TC87567 similar to GP|17065112|gb|AAL32710.1 Unknown protein {Arabidopsis
thaliana}, partial (82%)
Length = 2117
Score = 28.9 bits (63), Expect = 3.3
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +1
Query: 310 GAGKICWAKWSTVC 323
G G +CW KW T+C
Sbjct: 1141 GLGSLCWRKWKTLC 1182
>TC77553 homologue to GP|1066499|gb|AAB41904.1| NADH-dependent glutamate
synthase {Medicago sativa}, partial (37%)
Length = 3067
Score = 28.9 bits (63), Expect = 3.3
Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
Frame = -2
Query: 265 GGKVCLIKSVLSSIPLFYLS-FFKFPRGIAKECKRIQMDFLWGREVGA-GKICWAKWSTV 322
G VC K + +P + KF + K RI + W V A G++C+ KW+ +
Sbjct: 1215 GKVVCFKKPL*CFLPPILIH*LIKFRYFVPKRTSRIFLMTKWSTTVHATGRLCFQKWTRL 1036
Query: 323 C 323
C
Sbjct: 1035 C 1033
>TC82555 similar to PIR|T46017|T46017 hypothetical protein T10K17.160 -
Arabidopsis thaliana, partial (32%)
Length = 670
Score = 28.9 bits (63), Expect = 3.3
Identities = 21/63 (33%), Positives = 29/63 (45%), Gaps = 8/63 (12%)
Frame = +2
Query: 232 FVGANPRRRSMWEPLVCKMK------KRLQSWKQKMLSFGGKVCLIKSV--LSSIPLFYL 283
F R+R + L+C +K R SWK K L K CL+++ L + LFYL
Sbjct: 470 FKRVQKRKRLLMRILIC*LKCLLLIVMRSSSWKNKNLIGSIKKCLLEAFKQLRDVILFYL 649
Query: 284 SFF 286
F
Sbjct: 650 CNF 658
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.140 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,611,303
Number of Sequences: 36976
Number of extensions: 194317
Number of successful extensions: 1271
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 1259
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1267
length of query: 366
length of database: 9,014,727
effective HSP length: 97
effective length of query: 269
effective length of database: 5,428,055
effective search space: 1460146795
effective search space used: 1460146795
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0134.24