BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0132.5
(458 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC90408 similar to SP|O49543|NFS1_ARATH Cysteine desulfurase mi... 298 1e-84
TC89653 similar to SP|O49543|NFS1_ARATH Cysteine desulfurase mi... 278 2e-75
TC88912 similar to GP|16152176|gb|AAL14994.1 NIFS-like protein C... 92 5e-19
TC85689 homologue to SP|P50346|RLA0_SOYBN 60S acidic ribosomal p... 29 4.3
TC86086 similar to GP|15155442|gb|AAK86324.1 AGR_C_899p {Agrobac... 29 4.3
TC86118 homologue to GP|10864384|gb|AAG24097.1 C. elegans SRH-24... 28 5.7
BI271623 28 7.4
BQ751974 weakly similar to GP|9800311|gb|A pr95.1 {rat cytomegal... 28 9.7
TC77821 similar to GP|21618197|gb|AAM67247.1 putative aminotrans... 28 9.7
>TC90408 similar to SP|O49543|NFS1_ARATH Cysteine desulfurase mitochondrial
precursor (EC 4.4.1.-). [Mouse-ear cress] {Arabidopsis
thaliana}, partial (29%)
Length = 715
Score = 298 bits (763), Expect(2) = 1e-84
Identities = 149/179 (83%), Positives = 162/179 (90%)
Frame = +1
Query: 1 MTSKLLASTLRHHLTKPTQLLRRHFLSTAAAAAAAAEPQQHHDESAGLTMKGVKIAGRPL 60
MTSKL AS LR+HLTK L RRH STAAAAA + H + +AG+TMKGVKI+GRPL
Sbjct: 115 MTSKLFASLLRNHLTKKPHL-RRHPFSTAAAAALT---EPHEETTAGITMKGVKISGRPL 282
Query: 61 YLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGASPK 120
YLDVQATSPVDPRVLDAMLPFY+SRYGNPHSRTHFYGWESD+A+E+AR+QVA+LI ASPK
Sbjct: 283 YLDVQATSPVDPRVLDAMLPFYLSRYGNPHSRTHFYGWESDDAIEHARSQVAALISASPK 462
Query: 121 EIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPV 179
EIVFTSGATESNNISIKGVLHFYKEKK+HVITTQTEHKCVLDSCRHLQQEGFDVTYLPV
Sbjct: 463 EIVFTSGATESNNISIKGVLHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDVTYLPV 639
Score = 33.5 bits (75), Expect(2) = 1e-84
Identities = 15/16 (93%), Positives = 16/16 (99%)
Frame = +3
Query: 185 VDLEKLRAAIRPDTGL 200
VDLEKLR+AIRPDTGL
Sbjct: 657 VDLEKLRSAIRPDTGL 704
>TC89653 similar to SP|O49543|NFS1_ARATH Cysteine desulfurase mitochondrial
precursor (EC 4.4.1.-). [Mouse-ear cress] {Arabidopsis
thaliana}, partial (33%)
Length = 858
Score = 278 bits (712), Expect = 2e-75
Identities = 140/151 (92%), Positives = 147/151 (96%)
Frame = +1
Query: 308 ACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNLSFAYVEGESL 367
ACEVA+KEMEYDEKRISALQ+RLLNGIR+KLDGVVVNGSMERRY GNLNLSFAYVEGESL
Sbjct: 1 ACEVALKEMEYDEKRISALQQRLLNGIRDKLDGVVVNGSMERRYVGNLNLSFAYVEGESL 180
Query: 368 LMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEAEIDRAIQLT 427
LMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTE EIDRA+QLT
Sbjct: 181 LMGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEEEIDRAVQLT 360
Query: 428 VHQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
V QVEKLR+MSPLYEM+KEGINIKDI+W QH
Sbjct: 361 VQQVEKLRDMSPLYEMMKEGINIKDIKWDQH 453
>TC88912 similar to GP|16152176|gb|AAL14994.1 NIFS-like protein CpNifsp
precursor {Arabidopsis thaliana}, partial (52%)
Length = 991
Score = 91.7 bits (226), Expect = 5e-19
Identities = 60/213 (28%), Positives = 104/213 (48%), Gaps = 2/213 (0%)
Frame = +2
Query: 60 LYLDVQATSPVDPRVLDAMLPFYISRYGNPHSRTHFYGWESDEAVENARAQVASLIGASP 119
+Y+D ATS V+ A+ +Y N H H+ ++ + E+AR +VA+ I AS
Sbjct: 338 VYIDNAATSQKPAAVVKALQDYYEGYNSNVHRGIHYLSAKATDEYESARRKVAAFINASD 517
Query: 120 -KEIVFTSGATESNN-ISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYL 177
+EIVFT ATE+ N ++ L K+ V+T H ++ Q+ G + ++
Sbjct: 518 SREIVFTRNATEAINLVAYSWGLSNLKQGDEIVLTVAEHHSAIVPWQLVAQKVGAVLKFV 697
Query: 178 PVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDAAQA 237
+ D + D++KL+ + T +V+V V+N + P+ +I DA Q+
Sbjct: 698 NLNQDEIPDIDKLKEVLSKKTKIVAVHHVSNVLASVLPIRDIAHWAHGVGAKVLVDACQS 877
Query: 238 LGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALY 270
+ + DV+ + + S HK+ GP G+G LY
Sbjct: 878 VPHMVGDVQNLDADFLVASSHKMCGPTGIGFLY 976
>TC85689 homologue to SP|P50346|RLA0_SOYBN 60S acidic ribosomal protein P0.
[Soybean] {Glycine max}, partial (92%)
Length = 1392
Score = 28.9 bits (63), Expect = 4.3
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +2
Query: 29 AAAAAAAAEPQQHHDESAGLTMKG 52
AAAAAAAAEP++ D+ G + G
Sbjct: 1088 AAAAAAAAEPEEESDDDIGFGLFG 1159
>TC86086 similar to GP|15155442|gb|AAK86324.1 AGR_C_899p {Agrobacterium
tumefaciens str. C58 (Cereon)}, partial (2%)
Length = 1329
Score = 28.9 bits (63), Expect = 4.3
Identities = 21/70 (30%), Positives = 28/70 (40%), Gaps = 6/70 (8%)
Frame = +3
Query: 242 PVDVEKWNVSLMSL------SGHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSG 295
P V+ WNV+L SL S G KGV R P + P G G +
Sbjct: 927 PQPVKPWNVTLKSLLSLTSSSSSS*RGAKGVNFTASPRVPSLLPRPTCKGHGPFLVTKME 1106
Query: 296 TIPTPLVVGM 305
P P++ G+
Sbjct: 1107PPPLPVIAGL 1136
>TC86118 homologue to GP|10864384|gb|AAG24097.1 C. elegans SRH-245 protein
(corresponding sequence F31F4.9) {Caenorhabditis
elegans}, partial (3%)
Length = 1022
Score = 28.5 bits (62), Expect = 5.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -1
Query: 124 FTSGATESNNISIKGVLHFYKEKKRHVITTQTEH 157
+T+ T N K HF + +K H I T+TEH
Sbjct: 716 YTARTTSDNQR*FKTDFHFCQNRKFHSIMTETEH 615
>BI271623
Length = 317
Score = 28.1 bits (61), Expect = 7.4
Identities = 17/42 (40%), Positives = 19/42 (44%)
Frame = -1
Query: 149 HVITTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKL 190
H Q EH CVL HLQ G YL + S V E+L
Sbjct: 284 HPQRIQHEHMCVLPVVSHLQLRG*APLYLELNSSSQVLKEQL 159
>BQ751974 weakly similar to GP|9800311|gb|A pr95.1 {rat cytomegalovirus
Maastricht}, partial (3%)
Length = 553
Score = 27.7 bits (60), Expect = 9.7
Identities = 22/84 (26%), Positives = 38/84 (45%)
Frame = -3
Query: 257 GHKIYGPKGVGALYLRRRPRIRVEPQMNGGGQERGIRSGTIPTPLVVGMGAACEVAMKEM 316
GH+ P G + R R+R +GG + G+ + +P P+ VG AA
Sbjct: 503 GHRRAVPSAGGRV---RAVRVRRACAPDGGARVAGVGAARLPVPVGVGELAA-------- 357
Query: 317 EYDEKRISALQERLLNGIREKLDG 340
++ +A +E +L +R + DG
Sbjct: 356 ----RQGAARREHVLRDVRAEQDG 297
>TC77821 similar to GP|21618197|gb|AAM67247.1 putative aminotransferase
{Arabidopsis thaliana}, partial (93%)
Length = 1631
Score = 27.7 bits (60), Expect = 9.7
Identities = 36/141 (25%), Positives = 65/141 (45%), Gaps = 3/141 (2%)
Frame = +3
Query: 120 KEIVFTSGATESNNISIKGVLHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPV 179
KEI TSG TE+ ++ G+++ E VI + + + +T P
Sbjct: 531 KEITVTSGCTEAIAATVLGLINPGDE----VIVFAPFYDSYEATLSMAGAKVKGITLRP- 695
Query: 180 ESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIG-VFQPME--EIGRICKEFNVPFHTDAAQ 236
D + +E+L++ I +T + + +N G +F P E I +C E +V +D +
Sbjct: 696 -PDFALPIEELKSTISKNTRAILLNTPHNPTGKMFTPEELNTIASLCIENDVLVFSD--E 866
Query: 237 ALGKVPVDVEKWNVSLMSLSG 257
K+ D+E ++S+ SL G
Sbjct: 867 VYDKLAFDME--HISIASLPG 923
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.134 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,384,959
Number of Sequences: 36976
Number of extensions: 156958
Number of successful extensions: 790
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 782
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 785
length of query: 458
length of database: 9,014,727
effective HSP length: 99
effective length of query: 359
effective length of database: 5,354,103
effective search space: 1922122977
effective search space used: 1922122977
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0132.5