BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0125.16
(229 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77681 similar to GP|22597156|gb|AAN03465.1 nucleolar histone d... 33 0.12
TC77148 ENOD20 32 0.20
TC80891 similar to GP|21622328|emb|CAD36969. probable isoamyl al... 31 0.34
BQ141734 homologue to PIR|T49109|T491 glycine-rich protein - Ara... 31 0.45
TC89100 similar to GP|21553849|gb|AAM62942.1 putative RING zinc ... 30 0.77
AW257087 homologue to GP|6683126|dbj KIAA0339 protein {Homo sapi... 30 1.0
TC79555 homologue to GP|10567859|gb|AAG18593.1 L8019.5 {Leishman... 29 1.3
TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - ... 29 1.7
CB891518 similar to GP|12643044|gb putative AT-Hook DNA-binding ... 28 2.2
TC89939 similar to GP|15289911|dbj|BAB63606. hypothetical protei... 28 2.2
TC79235 weakly similar to GP|7297741|gb|AAF52992.1| CG17108 gene... 28 2.2
CB894389 similar to SP|P27879|HS11_ 18.1 kDa class I heat shock ... 28 2.2
TC79359 weakly similar to PIR|S49915|S49915 extensin-like protei... 28 2.2
TC84806 similar to GP|13676415|dbj|BAB41198. hypothetical protei... 28 2.2
BQ750979 similar to GP|13883467|gb PE_PGRS family protein {Mycob... 28 2.9
TC90148 similar to GP|6403503|gb|AAF07843.1| putative DnaJ prote... 28 2.9
BG456045 28 2.9
TC88788 weakly similar to SP|P05790|FBOH_BOMMO Fibroin heavy cha... 28 2.9
BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xy... 28 2.9
TC90933 similar to SP|P27484|GRP2_NICSY Glycine-rich protein 2. ... 28 2.9
>TC77681 similar to GP|22597156|gb|AAN03465.1 nucleolar histone deacetylase
HD2-P39 {Glycine max}, partial (47%)
Length = 1233
Score = 32.7 bits (73), Expect = 0.12
Identities = 33/130 (25%), Positives = 56/130 (42%), Gaps = 4/130 (3%)
Frame = +2
Query: 103 DEEDPSEIEADVDAPVAPLPATAVDLTDV----ASSSRLVEPKREPVVPSASRRFSFTPS 158
++ D SE E + D+ +P + A ++ EPK+ +A + + T
Sbjct: 365 EDNDDSEEEGETDSEDEDIPLITTEQNGKPEPKAEELKVSEPKKADAKNAAPAKNAATAK 544
Query: 159 RGYRVELLVRVVEEDVLSEEVDVEMGSTRVVRRIVRKEVMDLDTEMITVDSDSDTDVDNY 218
V+++ E+ +E D E+GS+ D EM DSDS+ + D
Sbjct: 545 H---VKVVDPKDEDSDDDDESDDEIGSS--------------DDEMENADSDSEDEDD-- 667
Query: 219 SPSDEEEEEE 228
SDE++EEE
Sbjct: 668 --SDEDDEEE 691
>TC77148 ENOD20
Length = 1108
Score = 32.0 bits (71), Expect = 0.20
Identities = 22/68 (32%), Positives = 32/68 (46%), Gaps = 3/68 (4%)
Frame = +3
Query: 94 PPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVAS--SSRLVEP-KREPVVPSAS 150
PPSPP+ S P + +P +P P+ + + S S+ + P KR P PS S
Sbjct: 435 PPSPPTPRSSTPIPHPPRRSLPSPPSPSPSPSPSPSPSPSPRSTPIPHPRKRSPASPSPS 614
Query: 151 RRFSFTPS 158
S +PS
Sbjct: 615 PSLSKSPS 638
>TC80891 similar to GP|21622328|emb|CAD36969. probable isoamyl alcohol
oxidase {Neurospora crassa}, partial (12%)
Length = 766
Score = 31.2 bits (69), Expect = 0.34
Identities = 24/76 (31%), Positives = 31/76 (40%)
Frame = +1
Query: 82 PSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPK 141
P+ GP GPPS P S++ PS P AP TA +A +R P+
Sbjct: 352 PATPAGPPRPSGPPSTPPSAAVSSPPS--------PSAPSATTA----PLAPLTRQSAPR 495
Query: 142 REPVVPSASRRFSFTP 157
P P+ R S P
Sbjct: 496 SRPRGPAPRRTSSRRP 543
>BQ141734 homologue to PIR|T49109|T491 glycine-rich protein - Arabidopsis
thaliana, partial (4%)
Length = 1181
Score = 30.8 bits (68), Expect = 0.45
Identities = 12/31 (38%), Positives = 16/31 (50%)
Frame = -3
Query: 81 PPSVRCGPGTGRGPPSPPSSSSDEEDPSEIE 111
P +C TGRGP PP ++ + P IE
Sbjct: 240 PSDNKCSDATGRGPSHPPRPAASDRGPHGIE 148
>TC89100 similar to GP|21553849|gb|AAM62942.1 putative RING zinc finger
protein {Arabidopsis thaliana}, partial (44%)
Length = 1047
Score = 30.0 bits (66), Expect = 0.77
Identities = 12/39 (30%), Positives = 22/39 (55%), Gaps = 1/39 (2%)
Frame = -2
Query: 91 GRGPPSPPSSSSDEEDPS-EIEADVDAPVAPLPATAVDL 128
G PP+PP +E+ P+ + ++ P++P+PA L
Sbjct: 752 GINPPNPPKPGENEKFPNLAVAVGINPPISPIPAVKFPL 636
>AW257087 homologue to GP|6683126|dbj KIAA0339 protein {Homo sapiens},
partial (2%)
Length = 466
Score = 29.6 bits (65), Expect = 1.0
Identities = 33/133 (24%), Positives = 48/133 (35%)
Frame = +1
Query: 96 SPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPSASRRFSF 155
S SSSSD + SE EA V + TA ASS V+ + S+S +
Sbjct: 28 SSSSSSSDSDSDSESEAPAKKAVPVVTKTAPTHAKSASSKMEVDSDSDSDSDSSSSSSTS 207
Query: 156 TPSRGYRVELLVRVVEEDVLSEEVDVEMGSTRVVRRIVRKEVMDLDTEMITVDSDSDTDV 215
+ + + EE D + S + + S S +
Sbjct: 208 SSVSSVKKPAVSAAKATPAKKEESDSDSDSDS------SSSSSSSSSSSSSSSSSSSSSS 369
Query: 216 DNYSPSDEEEEEE 228
+ S SD+EEEEE
Sbjct: 370 SSSSNSDDEEEEE 408
>TC79555 homologue to GP|10567859|gb|AAG18593.1 L8019.5 {Leishmania major},
partial (1%)
Length = 1410
Score = 29.3 bits (64), Expect = 1.3
Identities = 25/95 (26%), Positives = 36/95 (37%)
Frame = +2
Query: 64 GRLTRGVLRRMELSFNLPPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPA 123
GRL + N PP P + P PP+S S P+ + + P + P
Sbjct: 1061 GRLPAATTSSPATNANSPPFPPASPSSSVPPAKPPTSKS-ARSPAPV*SRPSPPPSSSPG 1237
Query: 124 TAVDLTDVASSSRLVEPKREPVVPSASRRFSFTPS 158
+A ++SSR R + SRR T S
Sbjct: 1238 SA-----ASASSRCSASSRARPTSAGSRRTRTTAS 1327
>TC76523 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - alfalfa,
partial (69%)
Length = 1615
Score = 28.9 bits (63), Expect = 1.7
Identities = 16/41 (39%), Positives = 19/41 (46%)
Frame = +3
Query: 94 PPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASS 134
P P+SSSDEE E + D DA PA + SS
Sbjct: 144 PQRKPASSSDEESDEESDEDEDAKPVSKPAAVAKKSKKDSS 266
>CB891518 similar to GP|12643044|gb putative AT-Hook DNA-binding protein
{Oryza sativa}, partial (27%)
Length = 691
Score = 28.5 bits (62), Expect = 2.2
Identities = 20/90 (22%), Positives = 39/90 (43%), Gaps = 3/90 (3%)
Frame = -3
Query: 62 ERGRLTRGVLRRMELSFNL---PPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPV 118
ER + RG + ++F++ P S + G PP+P +S+ +P +
Sbjct: 494 ERIQTVRGPV*EKAITFSVMSSPDSTKITCGVNPVPPAPSASNCFFPEPGGLPLCFLGGA 315
Query: 119 APLPATAVDLTDVASSSRLVEPKREPVVPS 148
+ P + + DVA++ K ++PS
Sbjct: 314 SAFPESPAEAADVAATGEETGAKPRAMLPS 225
>TC89939 similar to GP|15289911|dbj|BAB63606. hypothetical protein~similar
to Arabidopsis thaliana chromosome 1 F18O14.3, partial
(23%)
Length = 978
Score = 28.5 bits (62), Expect = 2.2
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +2
Query: 84 VRCGPGTGRGPPSPP-SSSSDEEDPSEIEADVDAPVAPLP 122
V G T R PPSPP SS+++ D E ++ +A P
Sbjct: 59 VGLGESTSRSPPSPPYSSNNNNSDAGNFECNICFDLAQDP 178
>TC79235 weakly similar to GP|7297741|gb|AAF52992.1| CG17108 gene product
{Drosophila melanogaster}, partial (21%)
Length = 648
Score = 28.5 bits (62), Expect = 2.2
Identities = 16/41 (39%), Positives = 18/41 (43%)
Frame = -2
Query: 81 PPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPL 121
PP + GP G GPP PP S P D AP P+
Sbjct: 494 PPIMGRGPPLGPGPPPPPMPHSHHPPPPH---DPFAPPPPV 381
>CB894389 similar to SP|P27879|HS11_ 18.1 kDa class I heat shock protein
(Fragment). [Alfalfa] {Medicago sativa}, partial (41%)
Length = 189
Score = 28.5 bits (62), Expect = 2.2
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -3
Query: 87 GPGTGRGPPSPPSSSSDEEDP 107
GP R PPSPP SS E+ P
Sbjct: 169 GPYHRRSPPSPPPSSVQEDQP 107
>TC79359 weakly similar to PIR|S49915|S49915 extensin-like protein - maize,
partial (3%)
Length = 1170
Score = 28.5 bits (62), Expect = 2.2
Identities = 22/60 (36%), Positives = 26/60 (42%), Gaps = 5/60 (8%)
Frame = +3
Query: 81 PPSVRCGPGTGRGPPSPPSSSSDEED-----PSEIEADVDAPVAPLPATAVDLTDVASSS 135
PP+ P + P PS SS E PS DAP A LP++ T ASSS
Sbjct: 507 PPTAAVSPVSS--PVEAPSVSSPPEASSAGIPSSSATPADAPAATLPSSKSPGTSPASSS 680
>TC84806 similar to GP|13676415|dbj|BAB41198. hypothetical protein {Glycine
max}, partial (10%)
Length = 803
Score = 28.5 bits (62), Expect = 2.2
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +3
Query: 199 DLDTEMITVDSDSDTDVDNYSPSDEE 224
DLD M++ SD+ +VD SPSD E
Sbjct: 402 DLDKNMVSFTSDNQQEVDVKSPSDLE 479
>BQ750979 similar to GP|13883467|gb PE_PGRS family protein {Mycobacterium
tuberculosis CDC1551}, partial (1%)
Length = 728
Score = 28.1 bits (61), Expect = 2.9
Identities = 20/67 (29%), Positives = 24/67 (34%), Gaps = 8/67 (11%)
Frame = +3
Query: 94 PPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLV--------EPKREPV 145
PPSPPS P A AP PA A + ++ E R V
Sbjct: 66 PPSPPSPPQQPTMPPRRSGRTAAAAAPAPAPAPAAPSLPLKGCIICHQWHVRPEAHRHRV 245
Query: 146 VPSASRR 152
VP +RR
Sbjct: 246 VPGLTRR 266
>TC90148 similar to GP|6403503|gb|AAF07843.1| putative DnaJ protein
{Arabidopsis thaliana}, partial (36%)
Length = 894
Score = 28.1 bits (61), Expect = 2.9
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = -1
Query: 95 PSPPSSSSDEEDPSEIEADVDAPVAPLP 122
P+P S E P E E D++ P +PLP
Sbjct: 519 PNPKEFESPEPPPKEKENDLEPPWSPLP 436
>BG456045
Length = 656
Score = 28.1 bits (61), Expect = 2.9
Identities = 19/65 (29%), Positives = 30/65 (45%), Gaps = 2/65 (3%)
Frame = +1
Query: 67 TRGVLRRMELSFNLPPSVRCG--PGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPAT 124
T +RRM + ++R PG G P SPP+S + PS +A + + P+
Sbjct: 118 TTPFVRRMSFGTSNMRNIRPNGSPGNGNSPSSPPTSQNGRPVPSGRKASLVGSPSQGPSL 297
Query: 125 AVDLT 129
A L+
Sbjct: 298 AAVLS 312
>TC88788 weakly similar to SP|P05790|FBOH_BOMMO Fibroin heavy chain
precursor (Fib-H) (H-fibroin). [Silk moth] {Bombyx
mori}, partial (2%)
Length = 782
Score = 28.1 bits (61), Expect = 2.9
Identities = 22/76 (28%), Positives = 28/76 (35%), Gaps = 5/76 (6%)
Frame = -2
Query: 82 PSVRCGPGTGRGPPSPPSSSSDEEDPS-----EIEADVDAPVAPLPATAVDLTDVASSSR 136
P+ P R P P+S EDP E+EA+ D P PA S S
Sbjct: 373 PAPDPAPDPARDPAWEPASEPAPEDPDPDPDPEVEAEPDPEPDPAPAPEPAPPPPPSPSP 194
Query: 137 LVEPKREPVVPSASRR 152
P P P ++ R
Sbjct: 193 KPAPNPIPTPPKSNPR 146
>BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xylostella
granulovirus}, partial (42%)
Length = 909
Score = 28.1 bits (61), Expect = 2.9
Identities = 19/71 (26%), Positives = 24/71 (33%), Gaps = 1/71 (1%)
Frame = +1
Query: 81 PPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAP-LPATAVDLTDVASSSRLVE 139
PP P + PP PP E+ P E P P P A T +R
Sbjct: 229 PPHTPPDPTPPQPPPQPPKPPHHEKRPRTPEPPGGRPPPPTTPKQARPTTQERGGARGAR 408
Query: 140 PKREPVVPSAS 150
P+ P P +
Sbjct: 409 PRPPPAPPQGT 441
Score = 27.3 bits (59), Expect = 5.0
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +1
Query: 81 PPSVRCGPGTGRGPPSPPSSSSDEE 105
PP+ R G G RG P+PP E
Sbjct: 607 PPTPRPGGGGARGAPAPPPQKKQRE 681
>TC90933 similar to SP|P27484|GRP2_NICSY Glycine-rich protein 2. [Wood
tobacco] {Nicotiana sylvestris}, partial (44%)
Length = 368
Score = 28.1 bits (61), Expect = 2.9
Identities = 13/40 (32%), Positives = 20/40 (49%)
Frame = -2
Query: 95 PSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASS 134
P PP S P ++ +AP P+ TA+DL ++ S
Sbjct: 343 PPPPRS*PPPPPPPRLDP*TEAPSGPVTLTALDLPSLSDS 224
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,030,771
Number of Sequences: 36976
Number of extensions: 86094
Number of successful extensions: 802
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 712
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 776
length of query: 229
length of database: 9,014,727
effective HSP length: 93
effective length of query: 136
effective length of database: 5,575,959
effective search space: 758330424
effective search space used: 758330424
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0125.16