BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0121b.8
(368 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG452205 weakly similar to GP|1345502|emb TNP2 {Antirrhinum maju... 179 1e-45
BF650639 weakly similar to GP|22748425|g putative transposon pro... 169 1e-42
BF636730 40 0.001
TC91682 38 0.005
TC89338 37 0.012
BQ149799 33 0.13
TC85325 30 1.1
BG454038 similar to GP|21304447|emb HOBBIT protein {Arabidopsis ... 30 1.5
TC82466 weakly similar to GP|15623807|dbj|BAB67867. putative pM5... 30 1.9
BE324764 28 5.7
TC87305 homologue to SP|Q05006|CC22_MEDSA Cell division control ... 28 5.7
TC79284 similar to GP|10178077|dbj|BAB11496. gene_id:K19B1.7~unk... 28 5.7
TC83715 similar to GP|21304447|emb|CAD31951. HOBBIT protein {Ara... 28 7.4
BQ148411 similar to GP|18491247|gb| At2g44600/F16B22.9 {Arabidop... 27 9.7
BE202813 weakly similar to GP|18076679|em P70 protein {Nicotiana... 27 9.7
TC85407 SP|P28551|TBB3_SOYBN Tubulin beta chain (Fragment). [Soy... 27 9.7
BF648034 similar to GP|16604643|gb unknown protein {Arabidopsis ... 27 9.7
TC90663 weakly similar to GP|8777402|dbj|BAA96992.1 MtN3-like pr... 27 9.7
BI265351 27 9.7
>BG452205 weakly similar to GP|1345502|emb TNP2 {Antirrhinum majus}, partial
(16%)
Length = 619
Score = 179 bits (455), Expect = 1e-45
Identities = 89/168 (52%), Positives = 110/168 (64%), Gaps = 12/168 (7%)
Frame = +1
Query: 202 KLPSSHYETKKIVGRLGLKYEKIHACPNDCMLFRNELADKNVNKCIKCGASRW------- 254
K+P S+ +T+ ++ LGL Y KI ACPNDCML+ + KN C CGASRW
Sbjct: 118 KVPDSYNKTR*MIRDLGLDYSKIDACPNDCMLYWKD--HKNDISCHVCGASRWIESTTES 291
Query: 255 -----KNDEKKVPAKVLRYFPLKSRLQRLFMSSETSRLMRWHDKGRNKDGDLRHPADSEA 309
E KV K+LR+FPL RLQRLFM SET+ +RWH +KDG LRHPAD +A
Sbjct: 292 GQIEENKKEHKVSTKILRHFPLIPRLQRLFMCSETATSLRWHHDDCSKDGKLRHPADGQA 471
Query: 310 WKKFDDQHQEFAKDSRNVRLGLASDGFNPYSSMKVFDSTWPVILVPYN 357
WK F+ H EFA+DSRN+RLGLASDGFNP+ +M + WPV L+PYN
Sbjct: 472 WKDFNGHHPEFARDSRNIRLGLASDGFNPFRAMNLKYXAWPVXLIPYN 615
>BF650639 weakly similar to GP|22748425|g putative transposon protein {Oryza
sativa (japonica cultivar-group)}, partial (6%)
Length = 568
Score = 169 bits (429), Expect = 1e-42
Identities = 84/155 (54%), Positives = 99/155 (63%), Gaps = 12/155 (7%)
Frame = +2
Query: 223 KIHACPNDCMLFRNELADKNVNKCIKCGASRW------------KNDEKKVPAKVLRYFP 270
KI AC NDCML+ + KN C CGASR E KV K+LR+FP
Sbjct: 71 KIDACXNDCMLYWKD--HKNDISCHVCGASRXIESTTEXGQIEENKKEHKVSTKILRHFP 244
Query: 271 LKSRLQRLFMSSETSRLMRWHDKGRNKDGDLRHPADSEAWKKFDDQHQEFAKDSRNVRLG 330
L LQR FM SET+ +RWH +KDG LRHPAD +AWK F+ H EFA+DSRN+ LG
Sbjct: 245 LIPXLQRXFMCSETATSLRWHHDDXSKDGKLRHPADGQAWKDFNGHHPEFARDSRNIXLG 424
Query: 331 LASDGFNPYSSMKVFDSTWPVILVPYNLPPWLCMK 365
LASDGFNP+ +M + S WP IL+PYN PPWLCMK
Sbjct: 425 LASDGFNPFRAMNLKYSAWPXILIPYNFPPWLCMK 529
>BF636730
Length = 637
Score = 40.0 bits (92), Expect = 0.001
Identities = 24/76 (31%), Positives = 36/76 (46%), Gaps = 3/76 (3%)
Frame = +1
Query: 2 SRMWMDYDRVSKEYLDGVESFLDHAFRESNGETIP---CPCKKCVLHYVVNRAMAYEHLM 58
+RM D RV+ E+L GV F++ A + + + + CPCK C + +HL
Sbjct: 316 NRMRPDNSRVTDEFLAGVCEFVEFACAQDDFKKVGKLRCPCKICKCRKP*SVDDVMKHLC 495
Query: 59 VNGIIPSYDTWFCHGE 74
+ G Y W HGE
Sbjct: 496 MKGFKEDYYYWSSHGE 543
>TC91682
Length = 1093
Score = 38.1 bits (87), Expect = 0.005
Identities = 19/52 (36%), Positives = 30/52 (57%)
Frame = +2
Query: 147 QLMKDANEELYSGCKKFSKLSFVLHLYHIKRLFKWTNESFDALLGLLKDALP 198
+L++D+ LY GC KF++LS VL LY++K W+ L +K +P
Sbjct: 2 RLVRDSKIPLYDGCAKFTRLSTVLKLYNLKARNGWS**ELYRLANSIKRYMP 157
>TC89338
Length = 1088
Score = 37.0 bits (84), Expect = 0.012
Identities = 26/78 (33%), Positives = 39/78 (49%), Gaps = 3/78 (3%)
Frame = +3
Query: 2 SRMWMDYDRVSKEYLDGVESFLDHAFRE-SNGETI-PCPCKKC-VLHYVVNRAMAYEHLM 58
++ WM R+S EY +GV FL A + N E + PCPC C +++ HL+
Sbjct: 219 AKRWMKAYRLSAEYDNGVTEFLQFAEKNLPNTEGLFPCPCVSCGNRDPKLSKEEIKGHLV 398
Query: 59 VNGIIPSYDTWFCHGESI 76
GI +Y HGE++
Sbjct: 399 SVGICQNYTQ*IWHGETM 452
Score = 33.1 bits (74), Expect = 0.18
Identities = 22/66 (33%), Positives = 33/66 (49%), Gaps = 3/66 (4%)
Frame = +3
Query: 14 EYLDGVESFLDHAFRE-SNGETI-PCPCKKCV-LHYVVNRAMAYEHLMVNGIIPSYDTWF 70
EY +G+ FL A + N E + PCPC C +++ HL+ GI +Y W
Sbjct: 498 EYDNGMTEFLQFAEKNLPNTEGLFPCPCISCGNWDPKLSKEEIRGHLVSVGICQNYTEWI 677
Query: 71 CHGESI 76
HGE++
Sbjct: 678 WHGETM 695
>BQ149799
Length = 437
Score = 33.5 bits (75), Expect = 0.13
Identities = 19/42 (45%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = +1
Query: 3 RMWMDYDRVSKEYLDGVESFLDHAFRE-SNGETI-PCPCKKC 42
R WM R+S EY +GV FL A + N E + PCPC C
Sbjct: 265 RRWMKAYRLSVEYDNGVTEFLQFAEKNLPNTEGLFPCPCVSC 390
>TC85325
Length = 3584
Score = 30.4 bits (67), Expect = 1.1
Identities = 16/62 (25%), Positives = 29/62 (45%)
Frame = -2
Query: 127 DENLTHTSRNQSHPEVEKFEQLMKDANEELYSGCKKFSKLSFVLHLYHIKRLFKWTNESF 186
D LT T ++S + + + MK E++ SGC K + + ++H I R F
Sbjct: 1711 DFPLTLTENSKSRC*LSNYREYMKQPAEDVESGCAKINLYTLIIHRLVILRYIMLQKTGF 1532
Query: 187 DA 188
++
Sbjct: 1531 NS 1526
>BG454038 similar to GP|21304447|emb HOBBIT protein {Arabidopsis thaliana},
partial (7%)
Length = 641
Score = 30.0 bits (66), Expect = 1.5
Identities = 31/107 (28%), Positives = 48/107 (43%), Gaps = 5/107 (4%)
Frame = +1
Query: 120 GDIESNLDENLTH----TSRNQSHPEVEKFEQLMKDANEELYSGCKKFSKLSFVLHLYHI 175
G+ LD++L + TS + S E + +EQ + AN + G + S + I
Sbjct: 295 GNHNDILDDSLLNITSTTSTSSSTMEAKSYEQ--EAANIPV--GGQVLSSSKVITGASEI 462
Query: 176 KRLFKWTNESFD-ALLGLLKDALPEGEKLPSSHYETKKIVGRLGLKY 221
L + E+F A L +DAL KLP HY T ++ +G Y
Sbjct: 463 LTLLRVLGEAFKLACLYRCQDALETYXKLPHKHYNTGWVLXXVGKMY 603
>TC82466 weakly similar to GP|15623807|dbj|BAB67867. putative pM5 collagenase
{Oryza sativa (japonica cultivar-group)}, partial (29%)
Length = 1351
Score = 29.6 bits (65), Expect = 1.9
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +1
Query: 54 YEHLMVNGIIPSYDTWFCHGESINVLNRKEETNCREE 90
Y+H + + SYD C G I+ R EE N +E
Sbjct: 1159 YQHPQIERFVSSYDRHTCTGNHIDFKKRCEEANVAKE 1269
>BE324764
Length = 579
Score = 28.1 bits (61), Expect = 5.7
Identities = 14/45 (31%), Positives = 20/45 (44%)
Frame = -3
Query: 204 PSSHYETKKIVGRLGLKYEKIHACPNDCMLFRNELADKNVNKCIK 248
P+ Y +K+ L + Y K H N C + E D+N C K
Sbjct: 259 PTQSYNSKRK*SLLSISYTKFHNTINFC*IVIVEFGDENPRNCGK 125
>TC87305 homologue to SP|Q05006|CC22_MEDSA Cell division control protein 2
homolog 2 (EC 2.7.1.-). [Alfalfa] {Medicago sativa},
complete
Length = 1378
Score = 28.1 bits (61), Expect = 5.7
Identities = 27/115 (23%), Positives = 45/115 (38%), Gaps = 3/115 (2%)
Frame = +2
Query: 220 KYEKIHACPNDCMLFRNELADKNVNKCIKCGASRWKNDEKKVPAKVLRYFPLKSRLQR-- 277
+YEK+ + D+ N+ I R + +++ VP+ +R L +Q
Sbjct: 107 QYEKVEKIGEGTYGVVYKARDRATNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHRN 286
Query: 278 -LFMSSETSRLMRWHDKGRNKDGDLRHPADSEAWKKFDDQHQEFAKDSRNVRLGL 331
+ + R + D DL KKF D EFAKD R +++ L
Sbjct: 287 IVRLQDVVHSEKRLYLVFEYLDLDL---------KKFMDSSPEFAKDQRQIKMFL 424
>TC79284 similar to GP|10178077|dbj|BAB11496. gene_id:K19B1.7~unknown
protein {Arabidopsis thaliana}, partial (56%)
Length = 1009
Score = 28.1 bits (61), Expect = 5.7
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 5/50 (10%)
Frame = +1
Query: 246 CIKCGASRWKNDEKKVP-AKVLRYFPLKSRL----QRLFMSSETSRLMRW 290
C++C SRW N + ++ R F + L QR+ MS+ TSR RW
Sbjct: 106 CVRCLLSRWLNILLSLLLSRCRR*FNHRQGLRVLRQRMMMSARTSRCCRW 255
>TC83715 similar to GP|21304447|emb|CAD31951. HOBBIT protein {Arabidopsis
thaliana}, partial (22%)
Length = 728
Score = 27.7 bits (60), Expect = 7.4
Identities = 31/114 (27%), Positives = 49/114 (42%), Gaps = 5/114 (4%)
Frame = +2
Query: 110 QSMGNNISDGGDIESNLDENLTH----TSRNQSHPEVEKFEQLMKDANEELYSGCKKFSK 165
QS N DGG +D + + TS + + E + +EQ DA SG + S+
Sbjct: 32 QSWANENMDGGIHNDAVDVSRLNISSTTSCSSAAVEAKAYEQ---DATTLQVSG-QATSE 199
Query: 166 LSFVLHLYHIKRLFKWTNESFD-ALLGLLKDALPEGEKLPSSHYETKKIVGRLG 218
+ I + + E + A L K+AL KLP HY T ++ ++G
Sbjct: 200 SKVITGASEILTILRVLGEGYRLACLYRCKEALDTYLKLPQRHYNTGWVLSQVG 361
>BQ148411 similar to GP|18491247|gb| At2g44600/F16B22.9 {Arabidopsis
thaliana}, partial (7%)
Length = 659
Score = 27.3 bits (59), Expect = 9.7
Identities = 11/41 (26%), Positives = 25/41 (60%)
Frame = -1
Query: 108 FNQSMGNNISDGGDIESNLDENLTHTSRNQSHPEVEKFEQL 148
F + G ++DGGD +++ D+ ++H S ++ KF+++
Sbjct: 452 FFRRAGTVVADGGDADASTDDEISHDS*SEFVLVRNKFDKI 330
>BE202813 weakly similar to GP|18076679|em P70 protein {Nicotiana tabacum},
partial (20%)
Length = 549
Score = 27.3 bits (59), Expect = 9.7
Identities = 19/88 (21%), Positives = 43/88 (48%), Gaps = 2/88 (2%)
Frame = +3
Query: 74 ESINVLN--RKEETNCREEAFRGDDMVGLIHDAFEGFNQSMGNNISDGGDIESNLDENLT 131
E+I+V+ R E ++C+E R +++G + E N+++ SDG +++
Sbjct: 78 EAISVMEKLRNEASDCKESESRALEVIGKMQMQLETVNKTVETLRSDG--LKATEAYKSL 251
Query: 132 HTSRNQSHPEVEKFEQLMKDANEELYSG 159
QS + + E+L++ + + +G
Sbjct: 252 ALELEQSRSQAKSLEELVRKLDTDSTNG 335
>TC85407 SP|P28551|TBB3_SOYBN Tubulin beta chain (Fragment). [Soybean]
{Glycine max}, partial (54%)
Length = 766
Score = 27.3 bits (59), Expect = 9.7
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +3
Query: 35 IPCPCKKCVLHYVVNRAMAYEH 56
I C KCV+H+ V R A+EH
Sbjct: 465 IACKDFKCVIHWEVARVQAWEH 530
>BF648034 similar to GP|16604643|gb unknown protein {Arabidopsis thaliana},
partial (28%)
Length = 652
Score = 27.3 bits (59), Expect = 9.7
Identities = 14/39 (35%), Positives = 20/39 (50%)
Frame = -1
Query: 94 GDDMVGLIHDAFEGFNQSMGNNISDGGDIESNLDENLTH 132
GDD+ G+ F+GF +M N SD D+ + TH
Sbjct: 157 GDDIFGVCFFTFKGFRGAMTINSSDRMDLRR*IIGTTTH 41
>TC90663 weakly similar to GP|8777402|dbj|BAA96992.1 MtN3-like protein
{Arabidopsis thaliana}, partial (7%)
Length = 709
Score = 27.3 bits (59), Expect = 9.7
Identities = 14/32 (43%), Positives = 20/32 (61%)
Frame = +2
Query: 186 FDALLGLLKDALPEGEKLPSSHYETKKIVGRL 217
F LLG+ K+ P G K PSS ++ + I GR+
Sbjct: 176 FSILLGI-KNC*P*GYKAPSSDFKVRSIAGRI 268
>BI265351
Length = 662
Score = 27.3 bits (59), Expect = 9.7
Identities = 11/28 (39%), Positives = 16/28 (56%)
Frame = -2
Query: 242 NVNKCIKCGASRWKNDEKKVPAKVLRYF 269
NVN+C K WK++ KK K+ R +
Sbjct: 163 NVNQCFKFENQSWKHNHKKNTMKIQRNY 80
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.136 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,930,828
Number of Sequences: 36976
Number of extensions: 183151
Number of successful extensions: 1013
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 999
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1006
length of query: 368
length of database: 9,014,727
effective HSP length: 97
effective length of query: 271
effective length of database: 5,428,055
effective search space: 1471002905
effective search space used: 1471002905
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0121b.8